LOCUS       sequence-220019-       13133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to early 
            endosome associated 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13133)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 13133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-EEA1
FEATURES             Location/Qualifiers
     source          1..13133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4957..4977)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4985..5001)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5017..5151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5041..5060)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5095..5114
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5590..5777,5778..5789)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5823..5839)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5823..5839)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5876..5896)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5876..5894)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5915..5931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5928..5950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5939..5955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5963..5969,5970..5987,5988..5993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6008..6029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6146..6163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6317..6905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6397..6416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7076..7867,7868..7936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7699..7718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7937..8041)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8204..8225)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(8574..8585,8586..8748)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     9121..9137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9157..9177
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtcgactta agacgaatat tacagatgac
      781 tcctgggaaa ggaggctctc agagctcgga gtctgagcag ccgtctgctg atatgaataa
      841 tgagtcctca gagggcttta tttgtcccca gtgtatgaaa tcccataact ctgctgagga
      901 gctcttcaaa cattatgagg tctttcatga accaggagac cagccgtccc cctttagccc
      961 cggcagggag gatttaacat tacttcgaca ggaaatccaa gacttacagg cttcactgaa
     1021 ggaggaacgc tggttttctg aggaattgaa aaaagaatta gacaaagtac aaggacgaat
     1081 agcaactaat aaaggcttac agagcgatgg tacaagtgga gaagatgcag agctcgagat
     1141 gagactcaat gaatcggaga cagagaagtt caacatcaaa cagatgaagg acctgtttga
     1201 acaaaaagct gctcagcttg cgaccgagat agttgacatc aaatctcgct acgatgagga
     1261 gaaaagtatg agagaagctt tggagcagag actggccaac ttaaaccaag atatgcaaaa
     1321 agaaaaacag gagaaggaga aactgtcggc agagctgctc cagaggccgg gtgtggaaga
     1381 tgtggaggtg ttgaagaaag agctggtgcg ggtgcagacg ctcatggaca gcatgacccg
     1441 agagcgagag gaggagtcag agcgcctcaa gggccaatat gagcagctgc aggccaactt
     1501 cactacctca gagaaaacga ttgctcaatt aaaggctgaa ctggagaagg gtcctcaaga
     1561 agcagcggtt tacacacaac agatccacca attacagagc agcttgaaca acctccagca
     1621 gcaatcccag gctttgtcag agaaattatc tcgtaaagag aaggagaatc aggaactgga
     1681 ggagcgtctg ggtcacgaac aggcctctaa gaagagtctt caggctaatc tgcatcagaa
     1741 agagctggag ctccaggaga gtcgagcacg ggtgtcttca ggagaagcag ctctcagtcg
     1801 agcacaggct gaattaactg aacgtgggga agaggctgca cgactcagac gagagctggg
     1861 cgagctggag aagaaccagc aggagctgaa ggcagagcgg aaacaacttc aacagcagag
     1921 ggaggataaa gaaaaccagg gcctgcagca acagagtgag atcagccagc tgcatgcgaa
     1981 gctgctggaa gcggagcgtc aggtggggga gctgcagggt cgtctgaaag agcagagaca
     2041 gctgtctgga gagaagctca aagacagaga acagcaggca gcagatctcc agctcaaact
     2101 ctcccgctta gaggaagagt taaaggagag ctccaccaag tccactgacc tgcagcatca
     2161 gctggacaaa tccaaacagc agcatcaaga actacaaaca cttcaacaga gcactaatgg
     2221 caaacttaga gaagcacaga atgacctgga gcaggtgttg cgtcagatcg gcgacaaaga
     2281 ccagaagatc cagaatcttg aagctctgct ccagaagagt aaggaaagtg tgagccagct
     2341 ggagaccgag agagaggatc tgtgtgccaa gattcaggct ggagaaggag aagctgcttt
     2401 gctgaaccaa ttacaagaga aaaatcactc gctacaagaa cagatcacgc agcttacaga
     2461 caagcttaag aaccagtcgg agagccacaa gcaggctcag gacaaccttc atgaacaggt
     2521 gcaggagcag aagacacacc tgcgttcagc tcaagaccgc tgtcagggtc tggagaccac
     2581 cgtcactgaa ctcaacacac agctcacaga gagcagagag aagatcgctc agctagacac
     2641 tcagttgaaa gccaagactg agatgctgtt gtctgctgaa gcagcaaaaa atgcccagag
     2701 agctgacttg gagagccacc tagagactgc tcagaatgca ttgcaggata agcagcaaga
     2761 gctcagtaaa gtgcaggctc agctggagga gcaggcccgc aggctgacgg agaagcagga
     2821 gcagtgctct cagctggaga acggcctgaa ggacagcaga gacaaactga tgactgcaga
     2881 acagaggata gagacgctcc agacccagac caagaaagcc gaggttgagt tgactgagct
     2941 ccgctccgga agagaacagg ctcagaaaga gcagtcttct cttaagaagc agatcagtga
     3001 gctggagatg aagaccaaag agttgaaccg acttttagat tccgagaaac aaggagcttc
     3061 cacccagcaa gaggagctaa agaagaaaag ctcagctctc accgaaacaa gacaaaagct
     3121 ggagaaagca gagcaggagc gtggagccct gcaggccaac ctggataaac tgtctcagga
     3181 gggtcagaag cagcaggcag agttggacaa gaaggtgcag ggtctgagtt cagatctcca
     3241 gaaaactcag ggggagaaag aagcgctggt aaaagaagtg gcgacagtga aagaggctct
     3301 gagtaaagct tctaaagccc tgaaggagag ccagactcaa ttagacaagg agaagaaggg
     3361 cagcaagacc actttggagg agaaggaaaa ggtttttcaa aaagtacaac aagagcttca
     3421 gaagaactca gaggccactt ctaaagagct cagcaatgtc aaaggtctgc tggagaaatc
     3481 gatagaggca gaaaagagtc ttcaatctca gctgactgca ctgaacgctc agctgaagca
     3541 gtgtcaggat acactgaagg agaaagaaaa gagtgaacag cagctgcagg cagagctgaa
     3601 gaccaggcaa ggctccttca gtcaggaagt gaagaaactg aagacacagg tgtctgagct
     3661 gcaggcttcc cttgctaaaa agactgaaga ggaagccaag caaaaggagc aaatcacagc
     3721 actgtctaaa gatctgagct cagagaagaa tcgctgtgca gagctgcaga aaaccagtga
     3781 cggcaccaaa gagagtctca ccgcccttca gtctgattac tatggcaaag agtctgagct
     3841 ctctgctgta cgccaggacc ttaaggtgtg cgaggagaag ttggttcttg ctcaggagga
     3901 gctggtgacc aacaggaacc agctcagtgc ttacgaaacc caaatccagg agctgaagac
     3961 gggccacaca gcgatggaga tagacctgag caagagagac gagaagatca aacagcaagt
     4021 ggagactctg cagaagctcc agaaacaaca gggacaaaca gaggagcagc tgaagaagga
     4081 gaaagctcaa tgcgaggagc tgagagagag tcaaagcgcc ctggaaaagg acaaaaacaa
     4141 actgtctgct gatctcaaaa cactggctga gaagaatgag aaggagctga aggacttgca
     4201 ggcagcaaag cagttgttga tccagcaaaa ggtggagatg caggggaagg tagaggcggc
     4261 tcaagcatct gtggagaaag aacagagaga gcatcagaag accagagact ccatcaaaca
     4321 gaatgagcag cagctcaagg ctgagaccaa caaaataaag cagcagctgg catcagaagt
     4381 gaaggctaaa gaggagatgg tgcggcagag ggaagaggct gaggtgaaga tgtccatgca
     4441 ggtgacggca ctgaatgaga acgtggccac gctgaagcga gagtggcaga gcagccagag
     4501 gcgggtgggt gagctggaga aacagacgga tgagctgaga ggagagattg ccgtgctgga
     4561 agcaacagtc cagaacaacc aagacgaaag acgtgcacta cttgagaggt gcgtccaggg
     4621 tgaaggggag atggagaagc ttcaggctaa gatgttggag atgcgcagga agttggacga
     4681 cactactgcc gccatgcagg agcttggtcg agagaaccag tccttacaga taaaacagtc
     4741 acaatccctc acacggaagt ggacagaaga ccatgaggtg cagaactgca tggcttgtgg
     4801 gaaaggcttc tccgtcactg tcagaaagca ccactgcaga cactgcggga acatcttctg
     4861 tgcagagtgc tctgcccgca acgccctcac accctcctcc aagaagcccg ttcgagtgtg
     4921 tgacaactgc tttgatgagc tccagggctg actagtcaac tttattatac atagttgata
     4981 attcactggc cgtcgtttta cggtaccatc gatgatgatc cagacatgat aagatacatt
     5041 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     5101 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     5161 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     5221 taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat ctgcgaagat
     5281 acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg
     5341 atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt
     5401 ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac
     5461 caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa
     5521 taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa
     5581 aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg
     5641 agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt
     5701 ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag
     5761 taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag
     5821 cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     5881 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6721 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6961 gttaaggcat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8041 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     8101 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8161 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     8221 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     8281 tggcccacgc tggctaagaa ctcatcagcc tccccggtcc atctacccac gtaccaatgc
     8341 accaattggc cacaatgacg gctactacat ggtgccattc cttcctcttt ataggaatgg
     8401 agactacctc ctgtccaaca atgctcttgg atacgagtac gcctacctgt tggacccagg
     8461 tcattgcaca acaccagaaa tgccctctga tctgcaaaag acgtgaatat ctgttcagac
     8521 acccatatcc actctgttcc acacaggtca gaggtttgtc caggagttct tgacagaggt
     8581 gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa gtacttaggg aaaattttac
     8641 tcaattaaaa gtaaaagtat ctggctagaa tcttacttga gtaaaagtaa aaaagtactc
     8701 cattaaaatt gtacttgagt attaaggaag taaaagtaaa agcaagaaag aaaactagag
     8761 attcttgttt aagcttttaa tctcaaaaaa cattaaatga aatgcataca aggttttatc
     8821 ctgctttaga actgtttgta tttaattatc aaactataag acagacaatc taatgccagt
     8881 acacgctact caaagttgta aaacctcaga tttaacttca gtagaagctg attctcaaaa
     8941 ttgttagtgt caagcctagc tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc
     9001 ctctcacagg cagccgatgc gggaagaggt gtattagtct tgatagagag gctgcaaata
     9061 gcaggaaacg tgagcagaga ctccctggtg tctgaaacac aggccagatg ggccctcgag
     9121 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttggca
     9181 cgcgcagatt ccgtgtgggc ctggtaatat cttaccattt taagctattt taagattagg
     9241 gaaaaaaatt aaataaaact actttaaaat tactttcctt aaaaagcaac atatttattt
     9301 agtttttgtt taactggctc aagattgtaa tattttactt ttacatgttt ttttaagtac
     9361 ctaaatgtgt tcaaaagtaa agtatgttat tgtttttttt ttttaatgtt agctaagctt
     9421 taaattgtaa tatattactt ttaaaagtga ctttttaaaa atgtaaataa aaaagtaact
     9481 ataaatgtgt aatgcatgtt atttctgtgg gtttttaatt ttcatctcaa ctgggcttac
     9541 aattttaaca taataaaaat caaatcaaat caaatcaggc ccttatttgt cacatacact
     9601 cttgtatact gaaataaact aaatgttgag aaaaatttaa atacaccaaa ataacgcata
     9661 ccttttttgt aattacctaa gtaaaacatt tagttccttt tttaagtgac tcaagattgt
     9721 aatctattaa ttgtaaaaga taaaactagt gatcaatctg acgtgcttct atgcagcctg
     9781 tgagattaat tgtgatgttt aattattaaa caatttaaat ggatcaaaca tttcacatgt
     9841 gcttattgtg tccaacaaaa ccagcagaag cacttaaaaa tattgcatga caacatgagt
     9901 ttttaatgta aattatatat acttcctaaa cgaagttaat catatttcta aaataataat
     9961 gtcattgaaa tatttaaata aatgataagc tttagttgat tcaacataaa attgaaagca
    10021 ataaggactt ttttttttta cagcggtgaa gcaaaagcat gtaataatca tataatataa
    10081 taaataaata aataaataat ggctcttggt gcaaatatga acactttaaa taaatataga
    10141 ataaatatga tcaataagtc ataacgatgt gttttatgtt gattataatt taaacatgtt
    10201 tctaacgcaa gttttaaaaa ctgttttaaa gtattatttt aagttataag atcaatttga
    10261 ctcaacattt tgtagtgaaa atatgttgtg taaaattaat ataatataca ctataaaaat
    10321 gcagggttcc acacaataca ttaatgttgt cccaacacaa atcgattaag ttaacaaata
    10381 tatgtggatt gaacataaaa aattaagttg tcccaatgaa atctcaagaa ttgtgttgtt
    10441 tcagctcatt ttaaataagt agttttaaca agtaaaaaat aacatatttt tgagtgtgta
    10501 tatatatata ttacaaaata taataatata aaatgtaaaa gatataaacc atgctacata
    10561 tatttaagag ctattttgca cattactttt ttgcaagcca ttttagtgtt ttttttacaa
    10621 atatatatta gctaaatata caatatctta aatatacaat taatcagaca tgcaattaat
    10681 gcatttaacc tacataaaaa tacatgatca acatatgttt tatgctgatt acaactaaat
    10741 caaacgtaat gaaattaatt tataaaagcg atttaaatat gtcaatattt taagttaaaa
    10801 tcacttgaaa cctactataa atgaacttaa attaaaaaaa tctcctttaa ttccaatgca
    10861 acagtgttgt tttttcagct gaggaccccc ggtgcagatg gttggccccc ggcccgctat
    10921 tctaaggaag cctttcttaa ttttagggcg gcgctgcatg cgctcagatc agattcacgg
    10981 gtggagaggg ttattttctc ttttttattt tttcttgcct gctcatattt gggcctgtgt
    11041 gaaccccagg ctccgcctcg gcccccagga gccgctctta tttaaagggg atcctcgcct
    11101 tcagtcttcg tcccagtgga agcatcagtc ttgtgataac aacttggtga gtcctacaga
    11161 ccagcgctcg actctaggct ttaaatgcat tgatttctct ggattagcta ccgggagagt
    11221 tttaactttg cttattagtc ttgtgctcga actgctaggt ttaaagagtg ttttctctaa
    11281 tgcatgcatg aagcgacagc tgaaaaccag aaatagagcg tttgtcctcc tgttaaactc
    11341 tagcctttct ttggcatttg tcagtccgag tcttcagctg ccggcaggag attgggtttc
    11401 agtaacactt gctgagcaca aactacacgt gctttcttca gatttaattc attattaatc
    11461 attctagatt taaatgatgt tcttactcat tgctgattat tttagccttg cggagcagat
    11521 taaatgagca cgtgtttcat taagattgat ttaaatataa ttaatatttc accactgtag
    11581 ttttaaaatt gccttctaag tcatggttga tttttaatag gtatcggctc taattagatt
    11641 actattttgg tttttaaatg ttatttctat ttcatttgta ggctcgtttt taatgtgatt
    11701 ttattagatt ctagttttaa aatcatctct tagccttaat gagcagactg aatgaacatg
    11761 tttcattaag attaacttca ttataatgaa ttatttaatc attctagtgg gttataaatc
    11821 agctctattt aaattaattg tcgtgtttat ttctatttaa tttttgatta ttttttcagg
    11881 gtcgttttta tgtgattttt tcccgtttct aattttaaac taattgtttg atttttatat
    11941 gattcgactc tttttaaatg aattgttttg ctttttttat tgagcatata tatattatac
    12001 aatttcaatc aacaattgtt gtgcttttta aatgttgttt ctatttcatt tctagggtcg
    12061 attttattcg attctagttt taaaatcgtg atttttaact tctaacctta ctgagcagac
    12121 tgaatgaaca tgtttcatca agattaactt cattatagtt aattattgaa tcgtgctagt
    12181 ggtttataaa tcagctctat ttaaattaat catcatcatt atttctattt aatttttatt
    12241 atttctttag gattattttt atgtgatttt tttcacgttc tagttttaaa ctcaatgctc
    12301 attactgctc acccttgctg agcagtcttt cgttaagatt aaattaatta ataaccattc
    12361 tagttttaaa acagtgtctt aaggcatagc tgatatttta caagtttact atttaatata
    12421 tctcctttct ttttttaggg tcgatttcat ctgatttgat taatttttat tatcttcact
    12481 aagctttttg ttctaggttt tattctcagt attattactg ttatatttga actgaatttc
    12541 tcctcatagt ttattcattt tatttattta ttttttgttt attcgctatt ttgtatttta
    12601 gtttttaatt agaaatttta ttagaatcgt tttgaactag tttttatgca ctctaacatt
    12661 aatattaaat cataactatt atttttatta taatttattt gtgtttatct ttttgtgtag
    12721 ctttcatgtg tttttatttt agttttatta tgtttgcatt gaattttttg gactagttta
    12781 tataacgata tacagaaaag ccatgacaac aaatgttttt ataataccta ttaagtataa
    12841 gctaatcaga tttccactat tctgttcaaa gttaacatta ttagccattc tgatggatca
    12901 tttgacattt gtcatatgtc tttatgaaca ctgctctttc agatgccact tttaaagctg
    12961 ttttttacag taaatgaacg tttattatta tgacattatt tcctaaggcc tctagagtac
    13021 agcaggatgt ggcgtgtgca ttaccgtgta attatgctga ctgtaaatgt gcatttatag
    13081 actgtcagca tgtctgactg tgcgctgttg tgattggtcc tgcacagacc aag
//
