LOCUS       sequence-220038-        8239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pCAG-Brainbow3.0
FEATURES             Location/Qualifiers
     source          1..8239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1745..1769
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1818..1851)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(1860..1893)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      1902..1911
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1908..2615
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    2797..2918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2931..2964)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2973..2982
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2979..2981,2982..2984,2985..3695)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2979..2981,2982..2984,2985..3695)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3024..3045)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3285..3304)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3632..3653
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3877..3998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4015..4048)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      4057..4066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4063..4758
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    4940..5061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5074..5107
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(5146..5170)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5261..5280)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5326..5381
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5380..5399)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5742..5758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5742..5758)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5755..5777)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5766..5782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5790..5796,5797..5814,5815..5820))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5835..5856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5915..6110
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5961..6096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6116..6250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6318..6335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6489..7077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6569..6588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7248..8039,8040..8108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7871..7890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8109..8213)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccagggggg gggggggggg gggggggggg
      541 gggggggggg gggggggggg gggcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg ggggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgagga
     1741 tatcacaagt ttgtacaaaa aagcaggctt taaaggaacg cacgtaacta taacggtcct
     1801 aaggtagcga acctaggata acttcgtata gcatacatta tacgaagtta tcggcgcgca
     1861 taacttcgta taggatactt tatacgaagt tatcaagcgc tgccaccatg gtgagcaagg
     1921 gcgaggagaa taacatggcc atcatcaagg agttcatgcg cttcaaggtg cgcatggagg
     1981 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     2041 gctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     2101 tcctgtcccc tcatttcacc tacggctcca aggcctacgt gaagcacccc gccgacatcc
     2161 ccgactactt caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaactacg
     2221 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     2281 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtgatg cagaagaaga
     2341 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggtgcc ctgaagggca
     2401 agatcaagat gaggctgaag ctgaaggacg gcggccacta cacctccgag gtcaagacca
     2461 cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcaagttgg
     2521 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     2581 gccactccac cggcggcatg gacgagctgt acaagaagct gaaccctcct gatgagagtg
     2641 gccccggctg catgagctgc tgtgtgctct cctgagcggc cgcgactcta gatcataatc
     2701 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     2761 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     2821 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2881 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtgctagc ataacttcgt
     2941 atagcataca ttatacgaag ttatctaacg ttgccaccat ggtgagcaag ggcgaggagc
     3001 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3061 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3121 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     3181 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3241 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3301 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3361 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3421 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     3481 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3541 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     3601 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3661 ccgggatcac tctcggcatg gacgagctgt acaagaagct gaaccctcct gatgagagtg
     3721 gccccggctg catgagctgc tgtgtgctct cctgagcggc cgcgactcta gatcataatc
     3781 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3841 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3901 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3961 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtgtttaa acatataact
     4021 tcgtatagga tactttatac gaagttatcg cgtacggcca ccatggtgag cgagctgatt
     4081 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
     4141 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg
     4201 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     4261 agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttcccc
     4321 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     4381 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     4441 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag
     4501 accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg
     4561 ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa acccgctaag
     4621 aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc
     4681 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     4741 agcaaactgg ggcacagaaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc
     4801 tgctgtgtgc tctcctgagc ggccgcgact ctagatcata atcagccata ccacatttgt
     4861 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     4921 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     4981 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5041 caaactcatc aatgtatctt aaggcgtact agtgaagttc ctattctcta gaaagtatag
     5101 gaacttccgt aactataacg gtcctaaggt agcgaatgca tctagaccca gctttcttgt
     5161 acaaagtggt gattcgcggc cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     5221 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     5281 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     5341 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     5401 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     5461 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     5521 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     5581 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     5641 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     5701 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5761 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5821 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5881 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     5941 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6001 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6061 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     6121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6241 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6301 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6901 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8221 ccccgaaaag tgccacctg
//
