LOCUS       sequence-220044-        9755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial vector for expression of Snap-tagged Staphylococcus aureus
            dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Savic N, Ringnalda FC, Lindsay H, Berk C, Bargsten K, Li Y, Neri D, 
            Robinson MD, Ciaudo C, Hall J, Jinek M, Schwank G
  TITLE     Covalent linkage of the DNA repair template to the CRISPR-Cas9 
            nuclease enhances homology-directed repair.
  JOURNAL   Elife. 2018 May 29;7. pii: 33761. doi: 10.7554/eLife.33761.
  PUBMED    29809142
REFERENCE   2  (bases 1 to 9755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-29"; volume: "7. pii: 33761. doi"; pages: " 
            10.7554/eLife.33761"
COMMENT     Sequence Label: pNS38-SadCas9-SNAP
FEATURES             Location/Qualifiers
     source          1..9755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..36)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        104..208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(209..277,278..1069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(427..446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1729..1748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1982..1999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2014..2153
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2079..2098)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2239..2261
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2255..2446)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3904..3923
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4007..4026
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4007..4025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4114..4136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4156..4173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4183..5283
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5257..5280
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5296..5316
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5320..8478
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MKRNYILGLAIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNE
                     GRRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEE
                     FSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGE
                     VRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFG
                     WKDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKF
                     QIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKE
                     IIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKA
                     INLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSI
                     KVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENA
                     KYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVK
                     QEEASKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRF
                     SVQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKER
                     NKGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEI
                     FITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKD
                     NDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKK
                     DNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKN
                     LDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLL
                     NRIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQ
                     IIKKG"
     CDS             8512..8538
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8518..8544
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             8557..8577
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8584..8604
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8638..9180
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for bacterial expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             9196..9216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9291..9309)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9305..9352
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(9718..9723,9724..9740,9741..9746))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca
     2101 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     2161 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2221 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2281 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
     2341 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     2401 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     2461 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
     2521 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     2581 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     2641 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
     2701 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     2761 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
     2821 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     2881 tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc
     2941 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     3001 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     3061 tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg
     3121 caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca
     3181 tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga tcgaagttag
     3241 gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct
     3301 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     3361 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
     3421 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     3481 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     3541 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     3601 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     3661 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt
     3721 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     3781 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     3841 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     3901 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     3961 cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact
     4021 atagggagac cacaacggtt tccctctagt gccggctccg gagagctctt taattaagcg
     4081 gccgccctgc aggactcgag ttctagaaat aattttgttt aactttaaga aggagatata
     4141 catatgaaat cttctcacca tcaccatcac catggttctt ctatgaaaat cgaagaaggt
     4201 aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
     4261 aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
     4321 aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
     4381 cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
     4441 caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
     4501 tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
     4561 ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
     4621 gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
     4681 ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
     4741 gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat
     4801 gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc
     4861 atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg
     4921 gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt
     4981 attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg
     5041 actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag
     5101 tcttacgagg aagagttggc gaaagatcca cgtattgccg ccactatgga aaacgcccag
     5161 aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact
     5221 gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag
     5281 actaatggga tcgaggaaaa cctgtacttc caatccaata tgaaaaggaa ttatatctta
     5341 ggattagcta tcggaattac atcagtgggt tatggaatta ttgattatga aactagagat
     5401 gtcatagatg cgggcgtacg tttatttaaa gaggctaatg ttgaaaataa tgaaggacga
     5461 cgatcaaaaa gaggtgccag aaggcttaag aggcgtcgta gacatagaat acaaagagta
     5521 aagaaacttt tatttgatta caatttgttg acagatcata gtgagctaag tggaatcaat
     5581 ccttacgagg cgcgcgtaaa gggattaagt caaaaattaa gtgaagagga attttctgcg
     5641 gcattgctac atttagcaaa gcgtagaggt gtacataatg ttaatgaagt ggaagaagat
     5701 acaggtaatg aattatccac taaagaacaa atttcaagaa atagtaaagc gttagaagag
     5761 aagtatgttg cagaattaca gttggaacgt ttgaaaaaag acggtgaagt gagaggttcg
     5821 attaaccgtt tcaaaacatc tgactatgta aaagaagcaa agcagttatt aaaagtacaa
     5881 aaagcatatc atcaacttga tcaatcattt atagacactt atattgattt attggaaaca
     5941 agaagaacat attatgaggg accaggtgaa ggtagcccat ttggatggaa agatattaaa
     6001 gaatggtatg aaatgttaat gggacattgt acgtatttcc cagaagaatt acgtagtgtg
     6061 aaatatgcct ataatgctga tttatataat gcgctgaatg atttgaacaa cttggttatt
     6121 acacgagatg agaatgagaa gctagagtat tatgaaaaat tccaaattat cgagaatgtc
     6181 tttaaacaaa agaaaaagcc gacgcttaaa caaattgcga aggaaatctt ggtgaatgaa
     6241 gaagacatca aaggctatcg tgtcacaagt acaggtaaac cagaatttac aaacttgaaa
     6301 gtttatcacg atatcaaaga tattacagca agaaaagaaa ttatcgagaa tgcagagcta
     6361 ctcgatcaaa tagctaaaat attaactatt taccagtcat cagaagatat acaagaagaa
     6421 ttaacaaacc taaattcaga attgacacaa gaagagattg aacaaatttc aaacttgaaa
     6481 ggttatacag gaactcataa cctttcacta aaggcaataa atttaatatt agacgaattg
     6541 tggcatacga acgataatca aatagctatt ttcaatcgtt tgaaacttgt acctaaaaag
     6601 gtagatttaa gccaacaaaa agaaattcct actactttag ttgatgattt tatactgtct
     6661 ccagtagtga aacgttcatt tatacaatct attaaagtta ttaacgctat tattaaaaaa
     6721 tacggtttgc caaatgatat tattattgaa cttgcgagag aaaagaattc taaagatgca
     6781 caaaaaatga ttaatgaaat gcagaagaga aatcgtcaaa cgaatgaacg tattgaggaa
     6841 attataagaa cgacaggtaa agaaaatgct aaatatttaa ttgaaaaaat taagctgcac
     6901 gatatgcaag aagggaaatg tttatactcg ttagaagcaa tccctctaga agatttactt
     6961 aataatccat tcaattacga agtagaccat atcattccac gttctgtttc tttcgataac
     7021 tctttcaata ataaagtgtt ggtgaaacaa gaagaagcta gtaaaaaagg taatagaacg
     7081 ccatttcaat atttaagttc ttcagattct aaaataagtt atgagacatt caaaaagcat
     7141 attttaaatc ttgctaaagg caaaggtaga atctctaaga cgaaaaaaga atatttgtta
     7201 gaagaacgag atatcaatcg cttcagtgtc caaaaagatt ttattaaccg taacttagta
     7261 gatacacgct atgcgacaag agggttaatg aacttattaa gatcttattt tagagtgaat
     7321 aacttagatg tcaaagtgaa atcgattaat ggcggattca caagtttctt aagaaggaaa
     7381 tggaagttca aaaaagaaag aaataagggc tacaaacacc atgctgaaga tgcactgatt
     7441 attgcgaacg ctgattttat tttcaaagaa tggaaaaaac tagataaagc taaaaaagtg
     7501 atggaaaatc aaatgtttga agaaaagcaa gctgaaagta tgcctgaaat tgagactgag
     7561 caagagtata aagaaatttt tataacgcct catcaaatta aacatattaa ggattttaaa
     7621 gattataaat attcacatag agttgataaa aagccgaata gagagttaat aaatgataca
     7681 ttatattcta cgagaaaaga tgacaagggt aatacattaa tcgttaataa cttaaatggt
     7741 ttatacgata aagataatga taaattgaaa aaattaatta ataaatcacc tgaaaaatta
     7801 ttgatgtatc atcatgatcc acaaacatat caaaaattaa aattgatcat ggaacaatat
     7861 ggcgatgaga aaaatccgct ttataaatat tatgaagaaa caggcaatta cttaacaaaa
     7921 tatagtaaaa aagataacgg accagtcatc aaaaaaatta aatattatgg taacaagcta
     7981 aatgcgcatt tagatattac ggatgattat ccaaatagca gaaataaagt agtaaaactt
     8041 tcattaaaac catatcgctt tgatgtttat ttagataatg gggtatataa atttgtgaca
     8101 gttaaaaatt tagatgttat caaaaaagaa aactactatg aagttaattc aaagtgttat
     8161 gaagaagcaa aaaaactgaa gaaaattagt aatcaagcag aatttatcgc aagtttttac
     8221 aataatgact tgattaagat taacggagaa ttatatagag tcataggtgt aaataatgat
     8281 ctacttaaca gaattgaagt aaatatgata gacatcacat atagagaata tttagagaac
     8341 atgaatgata aaagaccacc tagaataatt aaaacaatag caagcaaaac acaatctatt
     8401 aaaaagtatt ctacagatat tctaggcaat ctttatgaag tgaagagtaa aaagcatcct
     8461 caaatcataa agaaaggata cgaacattgg aagtggataa cggatccagc ctatccctat
     8521 gacgtgcccg attatgccag cctgggcagc ggctccccca agaaaaaacg caaggtggaa
     8581 gatcctaaga aaaagcggaa agtggacggc attggtagtg ggagcaacgg cagcagcgac
     8641 aaagattgcg aaatgaaacg taccaccctg gatagcccgc tgggcaaact ggaactgagc
     8701 ggctgcgaac agggcctgca tgaaattaaa ctgctgggta aaggcaccag cgcggccgat
     8761 gcggttgaag ttccggcccc ggccgccgtg ctgggtggtc cggaaccgct gatgcaggcg
     8821 accgcgtggc tgaacgcgta ttttcatcag ccggaagcga ttgaagaatt tccggttccg
     8881 gcgctgcatc atccggtgtt tcagcaggag agctttaccc gtcaggtgct gtggaaactg
     8941 ctgaaagtgg ttaaatttgg cgaagtgatt agctatcagc agctggcggc cctggcgggt
     9001 aatccggcgg ccaccgccgc cgttaaaacc gcgctgagcg gtaacccggt gccgattctg
     9061 attccgtgcc atcgtgtggt tagctctagc ggtgcggttg gcggttatga aggtggtctg
     9121 gcggtgaaag agtggctgct ggcccatgaa ggtcatcgtc tgggtaaacc gggtctggga
     9181 cctgcaggcg ggtcacctaa gaaaaaacga aaagttgagt aaggtaccac gcgtgcgcgc
     9241 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     9301 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     9361 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
     9421 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
     9481 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
     9541 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
     9601 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     9661 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
     9721 aaagcttatc gatgataagc tgtcaaacat gagaa
//
