LOCUS       sequence-220063-        8875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6351 MSCV-CMV-CMV-Flag-HA-NSD3
FEATURES             Location/Qualifiers
     source          1..8875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1418..1797
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1798..2001
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1952..1972
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2003..2021
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2024..2042
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2052..2076
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2149..2158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2158..2181
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2194..2220
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2236..2260
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4196..4220)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4239..4738
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4274..4297)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4671..4690
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4759..5358
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4759..4778)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5255..5275
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    5944..5960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5968..5974,5975..5992,5993..5998))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6013..6034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6151..6168)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6322..6910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6402..6421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7081..7872,7873..7941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7704..7723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7942..8046)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8114..8132
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8170..8192)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8292..8311
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8505..8527
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8655..8674
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(8874..8875,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatccttgac attgattatt gactagttat
     1441 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     1501 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     1561 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     1621 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1681 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1741 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     1801 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     1861 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     1921 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     1981 gaggtctata taagcagagc tctccctatc agtgatagag atctccctat cagtgataga
     2041 gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
     2101 tttgacctcc atagaagaca ccgggaccga tccagcctcc ggggatctgc caccatggac
     2161 tacaaggatg acgatgacaa gctcgatgga ggatacccat acgatgttcc agattacgct
     2221 gctcgaggtt atcaaacaag tttgtacaaa aaagttggca tggatttctc tttctctttc
     2281 atgcaaggga tcatgggaaa cacaattcag caaccacctc aactcattga ctccgccaac
     2341 atccgtcagg aggatgcctt tgataacaac agtgacattg ctgaagatgg tggccagaca
     2401 ccatatgaag ctactttgca gcaaggcttt cagtacccag ctacaacaga agatcttcct
     2461 ccactcacaa atgggtatcc atcatcaatc agtgtgtatg aaactcaaac caaataccag
     2521 tcatataatc agtatcctaa tgggtcagcc aatggctttg gtgcagttag aaactttagc
     2581 cccactgact attatcattc agaaattcca aacacaagac cacatgaaat tctggaaaaa
     2641 ccttcccctc cacagccacc acctcctcct tcggtaccac aaactgtgat tccaaagaag
     2701 actggctcac ctgaaattaa actaaaaata accaaaacta tccagaatgg cagggaattg
     2761 tttgagtctt ccctttgtgg agacctttta aatgaagtac aggcaagtga gcacacgaaa
     2821 tcaaagcatg aaagcagaaa agaaaagagg aaaaaaagca acaagcatga ctcatcaaga
     2881 tctgaagagc gcaagtcaca caaaatcccc aaattagaac cagaggaaca aaatagacca
     2941 aatgagaggg ttgacactgt atcagaaaaa ccaagggaag aaccagtact aaaagaggaa
     3001 gccccagttc agccaatact atcttctgtt ccaacaacgg aagtgtccac tggtgttaag
     3061 tttcaggttg gcgatcttgt gtggtccaag gtgggaacct atccttggtg gccttgtatg
     3121 gtttcaagtg atccccagct tgaggttcat actaaaatta acacaagagg tgcccgagaa
     3181 tatcatgtcc agttttttag caaccagcca gagagggcgt gggttcatga aaaacgggta
     3241 cgagagtata aaggtcataa acagtatgaa gaattactgg ctgaggcaac caaacaagcc
     3301 agcaatcact ctgagaaaca aaagattcgg aaaccccgac ctcagagaga acgtgctcag
     3361 tgggatattg gcattgccca tgcagagaaa gcattgaaaa tgactcgaga agaaagaata
     3421 gaacagtata cttttattta cattgataaa cagcctgaag aggctttatc ccaagcaaaa
     3481 aagagtgttg cctccaaaac cgaagttaaa aaaacccgac gaccaagatc tgtgctgaat
     3541 actcagccag aacagaccaa tgcaggggag gtggcctcct cactctcaag tactgaaatt
     3601 cggagacata gccagaggcg gcacacaagt gcggaagagg aagagccacc gcctgttaaa
     3661 atagcctgga aaactgcggc agcaaggaaa tccttaccag cttccattac gatgcacaaa
     3721 gggagcctgg atttgcagaa gtgtaacatg tctccagttg tgaaaattga acaagtgttt
     3781 gctcttcaga atgctacagg ggatgggaaa tttatcgatc aatttgttta ttcaacaaag
     3841 ggaattggta acaaaacaga aataagtgtc agggggcaag acaggcttat aatttctaca
     3901 ccaaaccaga gaaatgaaaa gccaacgcag agtgtatcat ctcctgaagc aacatctggt
     3961 tctacaggct cagtagaaaa gaagcaacag agaagatcaa ttagaactcg ttctgaatca
     4021 gagaaatcca ctgaggttgt gccaaagaag aagatcaaaa aggagcaggt tgaaacagtt
     4081 cctcaggcta cagtgaagac tggattacag aaagggtcgg cggaccgggg agtgcagggc
     4141 tctgtcagat tcagtgacag ctccgtctcc gcagcgattg aggaaactgt ggactgccca
     4201 actttcttgt acaaagtggt tcgataacga attctaccgg gtaggggagg cgcttttccc
     4261 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     4321 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     4381 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     4441 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     4501 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     4561 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     4621 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     4681 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     4741 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     4801 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     4861 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     4921 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     4981 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5041 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5101 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5161 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5221 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     5281 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     5341 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     5401 cgaccccatg catcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     5461 aaagacccca cctgtaggtt tggcaagcta gagaaccatc agatgtttcc agggtgcccc
     5521 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     5581 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc
     5641 gccagtcctc cgatagactg cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     5701 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     5761 cccgtcagcg ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga
     5821 gggtaggagt cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata
     5881 ctccaatact cctgaaatag ttcattatgg acagcgcaga aagagctggg gagaattgtg
     5941 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6001 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6061 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6121 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6181 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6241 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6301 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6361 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6421 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6481 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6541 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6601 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6661 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6721 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6781 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6841 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6901 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6961 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7021 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7081 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7141 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7201 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7261 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7321 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7381 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7441 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7501 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7561 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7621 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7681 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7741 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7801 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7861 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7921 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7981 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8041 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     8101 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     8161 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     8221 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     8281 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     8341 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     8401 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     8461 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     8521 taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     8581 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     8641 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     8701 gtgatgccgg ccacgatgcg tccggcgtag aggcgattag tccaatttgt taaagacagg
     8761 atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa gcctatagag
     8821 tacgagccat agataaaata aaagatttta tttagtctcc agaaaaaggg gggaa
//
