LOCUS       sequence-220066-       11146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of N-terminally tagged 
            eGFP-SHLD2, m1 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11146)
  AUTHORS   Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling 
            AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato
            S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, 
            Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY,
            Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, 
            Lord CJ, Rottenberg S, Durocher D
  TITLE     The shieldin complex mediates 53BP1-dependent DNA repair.
  JOURNAL   Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. 
            Epub 2018 Jul 18.
  PUBMED    30022168
REFERENCE   2  (bases 1 to 11146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0340-7"; journalName: "Nature"; date: "2018-08";
            volume: "560"; issue: "7716"; pages: "117-121"
COMMENT     Sequence Label: pCW-eGFP-SHLD2-m1
FEATURES             Location/Qualifiers
     source          1..11146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..49
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(58..60,61..63,64..774)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(58..60,61..63,64..774)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(103..124)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(364..383)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     711..732
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3497..3521)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3539..3556
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3566..4076
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3995..4013
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4086..4682
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4086..4105)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4582..4602
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4683..4736
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4737..5354,5355..5360,5361..5396,5397..5399,5400..5435,
                     5436..5438,5439..5474,5475..5483)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5523..5543)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5941..5952)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6157..6390
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6462..6583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6499..6518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6553..6572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6623..6758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6758..6829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6830..6898,6899..7690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7048..7067)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7861..8449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8350..8369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8603..8620
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8737..8758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8773..8778,8779..8796,8797..8803)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8811..8827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8816..8838
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8835..8851
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8835..8851
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8870..8890
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8872..8890
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8918..9144
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9145..9325
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9372..9497
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9990..10223
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10408..10452
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10665..10782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10836..11146,1..4)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    10844..10862
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10880..10898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10915..10933
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10951..10969
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10987..11005
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11022..11040
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11058..11076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     join(11123..11146,1)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgcctggaga attggctagc atcaacaagt ttgtacaaaa aagcaggctc cggtaccatg
       61 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      121 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      181 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      241 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      301 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      361 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      421 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      481 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
      541 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      601 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      661 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      721 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcgcg
      781 ccaagtggag gatctcaagt ccacattttt tggggtgctc caattgctcc actgaaaatc
      841 acagtatcag aagacacagc ttctttaatg tctgttgctg acccctggaa aaaaattcag
      901 cttttataca gtcaacattc tttatatctg aaggatgaaa aacagcacaa aaatcttgaa
      961 aactataaag tcccagaatc tattggttct ccagatctta gtggtcattt cttagcaaac
     1021 tgtatgaata gacatgttca tgtgaaagat gactttgtac gttctgtttc tgaaacacag
     1081 aatatagaat cccagaagat tcactcctct agactgagtg atataactag ctctaatatg
     1141 caaatatgtg gatttaaaag cacagttccg catttcaccg aagaagaaaa gtatcaaaag
     1201 cttctcagtg aaaataaaat tagagatgaa cagcctaaac atcagccaga tatatgtggt
     1261 aagaacttta acacaaattt gtttcagttg ggccataaat gtgcagctgt gttggatttg
     1321 gtttgtagta ctgaaaaaat taatataggg cctgaagtgg tacaaagaga gtgtgtgcca
     1381 acagaatatc atgaaataca aaaccagtgt ttgggattat tttcctcgaa cgcagtagat
     1441 aagtcaaggt ctgaagcagc agttaggaag gtctcagacc ttaaaatatc aactgataca
     1501 gaatttctca gtataattac ctccagccag gttgcttttt tagctcaaaa gaaagataaa
     1561 aggcggagtc ctgtaaataa agggaatgta aacatggaga ctgaaccaaa ggcaagttac
     1621 ggggagataa gaatacctga agagaattcg attcagcttg atggttttac agaagcatat
     1681 gaaagtggac aaaaccaagc atattccctt gaacttttta gtcctgtttg tcctaaaaca
     1741 gaaaatagcc gcattcacat aaactctgat aaaggtcttg aagaacatac aggatctcaa
     1801 gaacttttca gttctgaaga tgaactgcca ccaaatgaga tacgtattga gttgtgtagc
     1861 tcaggaatac tgtgttccca actaaatacc ttccacaaaa gtgctattaa aagaagctgt
     1921 acctctgaag ataaagtggg ccagtctgaa gctctatcta gagtccttca agtagctaag
     1981 aaaatgaagt tgatttctaa tggaggagat tctgctgtag aaatggatcg gagaaatgtg
     2041 tctgaattta agagtattaa aaaaacatca ttaataaaaa actgtgattc taaaagccag
     2101 aagtataatt gtttagtcat ggtgctatct ccatgccatg tgaaggaaat aaacataaaa
     2161 ttcggaccaa attctggctc taaagtgcct ttagcaacag ttacagtaat tgatcaatca
     2221 gaaactaaga agaaggtttt tctggcgagg actgcagcag ctgcggcatt tacagtgttt
     2281 cttggagata taattttact cacagatgtt gttattcatg aggaccaatg gattggcgag
     2341 acagtactac aatcaacatt tagcagtcag ttattaaatc ttgggagtta ttcatctatt
     2401 cagcctgaag aatattccag tgtagttagt gaagttgtac ttcaagactt actggcatat
     2461 gtgtcctcaa aacattccta cctcagagat cttcctccga ggcagcctca gagggtgaac
     2521 agtatagact ttgtagaatt ggagcacctt caacctgatg tattagtcca cgcagtacta
     2581 agagttgttg atttcactat actgacagag gcagtataca gttatagagg acagaagcag
     2641 aaaaaagtta tgttaacagt ggaacaggcc caagatcaac attatgcgct tgtattatgg
     2701 ggtcctggag cagcctggta ccctcaactt caaaggaaaa aaggttatat ttgggaattt
     2761 aaatatcttt ttgttcagtg caattacaca ctagaaaacc tagaattgca tacaacgcct
     2821 tggtcatcct gtgagtgctt gtttgatgat gatataaggg caattacatt taaagcaaaa
     2881 tttcaaaaaa gtgcaccctc ctttgtgaag atatcagact tagcaaccca cctagaggat
     2941 aagtgttcag gagtggttct gattaaagcc cagatttcag agctggcatt tcctattaca
     3001 gcatctcaga agatagcgct aaatgctcac agttctctga agagtatttt ttcttctctt
     3061 cccaacatcg tatatactgg ttgtgcaaaa tgtggattgg aactagaaac agatgagaac
     3121 aggatctaca aacaatgttt tagctgcttg ccatttacta tgaagaaaat atattatagg
     3181 ccagcgttaa tgactgccat tgatggaaga catgatgttt gtatccgtgt agaatcaaag
     3241 ctgatagaga agattcttct caacatttct gcagactgcc tcaacagagt gatagttcct
     3301 tcctcagaga tcacctatgg gatggtcgtg gcagacctgt tccactcctt gttggcagtc
     3361 agcgcagaac cttgtgtatt aaagattcag agcctttttg tgttagatga aaacagctat
     3421 ccattacaac aagatttctc cctcctggat ttttatcctg acattgtaaa gcatggagcc
     3481 aatgcccgtc tctgagaccc agctttcttg tacaaagtgg tttagtaatg aaccggtcca
     3541 ccaccaccac caccactaag gatccggggt tggggttgcg ccttttccaa ggcagccctg
     3601 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     3661 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     3721 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     3781 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     3841 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     3901 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     3961 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     4021 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccagcaat
     4081 tcaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     4141 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     4201 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     4261 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     4321 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     4381 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     4441 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     4501 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     4561 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     4621 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     4681 ccgaaggtag aggttctctc ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt
     4741 ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta
     4801 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     4861 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     4921 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     4981 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     5041 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     5101 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     5161 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     5221 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     5281 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     5341 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     5401 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     5461 accttgacat gctccccggg taactaagta aggatcgatc caagatatcg tattcttaac
     5521 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5581 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5641 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5701 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5761 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5821 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     5881 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5941 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6001 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6061 gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac aaggcagctg
     6121 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6181 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6241 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6301 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6361 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6421 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6481 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6541 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     6601 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6721 gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt atccgctcat
     6781 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6841 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6901 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6961 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7021 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7081 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7141 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7201 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7261 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7321 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7381 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7441 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7501 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7561 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7621 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7681 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7741 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7801 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7861 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7921 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7981 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8041 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8101 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8161 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8221 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8281 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8341 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8401 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8461 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8521 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8581 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8641 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8701 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8761 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8821 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     8881 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     8941 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     9001 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     9061 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     9121 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     9181 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     9241 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     9301 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     9361 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     9421 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     9481 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     9541 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     9601 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     9661 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     9721 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     9781 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     9841 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     9901 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     9961 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    10021 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    10081 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    10141 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    10201 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    10261 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    10321 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    10381 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    10441 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    10501 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    10561 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    10621 ggacaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
    10681 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
    10741 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
    10801 agcagagatc cactttggcc gcgaatcgat atgtcgagtt tactccctat cagtgataga
    10861 gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt ttactcccta
    10921 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
    10981 gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca gtgatagaga
    11041 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac
    11101 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagat
//
