LOCUS       sequence-220072-       11155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of N-terminally tagged 
            eGFP-SHLD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11155)
  AUTHORS   Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling 
            AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato
            S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, 
            Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY,
            Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, 
            Lord CJ, Rottenberg S, Durocher D
  TITLE     The shieldin complex mediates 53BP1-dependent DNA repair.
  JOURNAL   Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. 
            Epub 2018 Jul 18.
  PUBMED    30022168
REFERENCE   2  (bases 1 to 11155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0340-7"; journalName: "Nature"; date: "2018-08";
            volume: "560"; issue: "7716"; pages: "117-121"
COMMENT     Sequence Label: pCW-eGFP-SHLD2
FEATURES             Location/Qualifiers
     source          1..11155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        214..528
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    258..276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    293..311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    329..347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    365..383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    400..418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    436..454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     501..525
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    549..573
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(591..593,594..596,597..1307)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(591..593,594..596,597..1307)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(636..657)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(897..916)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1244..1265
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4030..4054)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4072..4089
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4099..4609
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4528..4546
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4619..5215
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4619..4638)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5115..5135
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5216..5269
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5270..5887,5888..5893,5894..5929,5930..5932,5933..5968,
                     5969..5971,5972..6007,6008..6016)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6056..6076)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6474..6485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6690..6923
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6995..7116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7032..7051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7086..7105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7156..7291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7291..7362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7363..7431,7432..8223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7581..7600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8394..8982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8883..8902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9136..9153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9270..9291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9306..9311,9312..9329,9330..9336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9344..9360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9349..9371
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9368..9384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9368..9384
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9403..9423
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9405..9423
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9451..9677
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9678..9858
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9905..10030
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10523..10756
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10941..10985
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca ctttggccgc gaatcgatat gtcgagttta ctccctatca gtgatagaga
      241 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
      301 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      361 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
      421 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
      481 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattggc
      541 tagcatcaac aagtttgtac aaaaaagcag gcttcgaagg agatagaacc atggtgagca
      601 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      661 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      721 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      781 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      841 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      901 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      961 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1021 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1081 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1141 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1201 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1261 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc gcgccaagtg
     1321 gaggatctca agtccacatt ttttggggtg ctccaattgc tccactgaaa atcacagtat
     1381 cagaagacac agcttcttta atgtctgttg ctgacccctg gaaaaaaatt cagcttttat
     1441 acagtcaaca ttctttatat ctgaaggatg aaaaacagca caaaaatctt gaaaactata
     1501 aagtcccaga atctattggt tctccagatc ttagtggtca tttcttagca aactgtatga
     1561 atagacatgt tcatgtgaaa gatgactttg tacgttctgt ttctgaaaca cagaatatag
     1621 aatcccagaa gattcactcc tctagactga gtgatataac tagctctaat atgcaaatat
     1681 gtggatttaa aagcacagtt ccgcatttca ccgaagaaga aaagtatcaa aagcttctca
     1741 gtgaaaataa aattagagat gaacagccta aacatcagcc agatatatgt ggtaagaact
     1801 ttaacacaaa tttgtttcag ttgggccata aatgtgcagc tgtgttggat ttggtttgta
     1861 gtactgaaaa aattaatata gggcctgaag tggtacaaag agagtgtgtg ccaacagaat
     1921 atcatgaaat acaaaaccag tgtttgggat tattttcctc gaacgcagta gataagtcaa
     1981 ggtctgaagc agcagttagg aaggtctcag accttaaaat atcaactgat acagaatttc
     2041 tcagtataat tacctccagc caggttgctt ttttagctca aaagaaagat aaaaggcgga
     2101 gtcctgtaaa taaagggaat gtaaacatgg agactgaacc aaaggcaagt tacggggaga
     2161 taagaatacc tgaagagaat tcgattcagc ttgatggttt tacagaagca tatgaaagtg
     2221 gacaaaacca agcatattcc cttgaacttt ttagtcctgt ttgtcctaaa acagaaaata
     2281 gccgcattca cataaactct gataaaggtc ttgaagaaca tacaggatct caagaacttt
     2341 tcagttctga agatgaactg ccaccaaatg agatacgtat tgagttgtgt agctcaggaa
     2401 tactgtgttc ccaactaaat accttccaca aaagtgctat taaaagaagc tgtacctctg
     2461 aagataaagt gggccagtct gaagctctat ctagagtcct tcaagtagct aagaaaatga
     2521 agttgatttc taatggagga gattctgctg tagaaatgga tcggagaaat gtgtctgaat
     2581 ttaagagtat taaaaaaaca tcattaataa aaaactgtga ttctaaaagc cagaagtata
     2641 attgtttagt catggtgcta tctccatgcc atgtgaagga aataaacata aaattcggac
     2701 caaattctgg ctctaaagtg cctttagcaa cagttacagt aattgatcaa tcagaaacta
     2761 agaagaaggt ttttctgtgg aggactgcag cattttgggc atttacagtg tttcttggag
     2821 atataatttt actcacagat gttgttattc atgaggacca atggattggc gagacagtac
     2881 tacaatcaac atttagcagt cagttattaa atcttgggag ttattcatct attcagcctg
     2941 aagaatattc cagtgtagtt agtgaagttg tacttcaaga cttactggca tatgtgtcct
     3001 caaaacattc ctacctcaga gatcttcctc cgaggcagcc tcagagggtg aacagtatag
     3061 actttgtaga attggagcac cttcaacctg atgtattagt ccacgcagta ctaagagttg
     3121 ttgatttcac tatactgaca gaggcagtat acagttatag aggacagaag cagaaaaaag
     3181 ttatgttaac agtggaacag gcccaagatc aacattatgc gcttgtatta tggggtcctg
     3241 gagcagcctg gtaccctcaa cttcaaagga aaaaaggtta tatttgggaa tttaaatatc
     3301 tttttgttca gtgcaattac acactagaaa acctagaatt gcatacaacg ccttggtcat
     3361 cctgtgagtg cttgtttgat gatgatataa gggcaattac atttaaagca aaatttcaaa
     3421 aaagtgcacc ctcctttgtg aagatatcag acttagcaac ccacctagag gataagtgtt
     3481 caggagtggt tctgattaaa gcccagattt cagagctggc atttcctatt acagcatctc
     3541 agaagatagc gctaaatgct cacagttctc tgaagagtat tttttcttct cttcccaaca
     3601 tcgtatatac tggttgtgca aaatgtggat tggaactaga aacagatgag aacaggatct
     3661 acaaacaatg ttttagctgc ttgccattta ctatgaagaa aatatattat aggccagcgt
     3721 taatgactgc cattgatgga agacatgatg tttgtatccg tgtagaatca aagctgatag
     3781 agaagattct tctcaacatt tctgcagact gcctcaacag agtgatagtt ccttcctcag
     3841 agatcaccta tgggatggtc gtggcagacc tgttccactc cttgttggca gtcagcgcag
     3901 aaccttgtgt attaaagatt cagagccttt ttgtgttaga tgaaaacagc tatccattac
     3961 aacaagattt ctccctcctg gatttttatc ctgacattgt aaagcatgga gccaatgccc
     4021 gtctctagga cccagctttc ttgtacaaag tggtttagta atgaaccggt ccaccaccac
     4081 caccaccact aaggatccgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc
     4141 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
     4201 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
     4261 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
     4321 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
     4381 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
     4441 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
     4501 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
     4561 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagc aattcaccat
     4621 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4681 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4741 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4801 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4861 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4921 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4981 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5041 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5101 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5161 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccgaagg
     5221 tagaggttct ctcctcactt gtggtgatgt tgaagaaaac cctggtccaa tgtctagact
     5281 ggacaagagc aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg
     5341 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
     5401 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
     5461 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
     5521 gtcataccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
     5581 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
     5641 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
     5701 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
     5761 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
     5821 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
     5881 aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
     5941 ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga
     6001 catgctcccc gggtaactaa gtaaggatcg atccaagata tcgtattctt aactatgttg
     6061 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6121 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6181 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6241 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6301 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6361 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     6421 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6481 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6541 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgtttcgc
     6601 ctcggcgtcc ggactagagg tacctttaag accaatgact tacaaggcag ctgtagatct
     6661 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6721 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6781 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6841 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6901 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6961 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7021 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7081 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7141 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7201 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7261 cagaagtagt gaggaggctt ttttggaggc ctttcaaata tgtatccgct catgagacaa
     7321 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7381 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7441 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7501 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7561 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7621 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7681 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7741 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7801 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7861 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7921 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7981 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8041 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8101 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8161 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8221 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8281 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8341 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8521 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8761 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8821 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9001 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9061 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     9241 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     9301 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     9361 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     9421 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     9481 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     9541 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     9601 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     9661 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9721 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9781 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9841 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     9901 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     9961 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
    10021 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
    10081 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
    10141 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
    10201 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
    10261 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
    10321 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
    10381 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    10441 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    10501 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    10561 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    10621 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    10681 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    10741 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    10801 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    10861 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    10921 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    10981 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    11041 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    11101 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg agggg
//
