LOCUS       sequence-220075-       12505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for dox-inducible expression of BE3 (not codon 
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-TRE3G-BE3-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..12505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(68..87)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        259..638
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        640..838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     793..813
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             856..1036
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1083..1208
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1705..1938
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2123..2167
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2465..2582
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2643..3021
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2651..2669
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2687..2705
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2723..2741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2759..2777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2795..2813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2831..2849
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2867..2885
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2930..2954
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3048..3734
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(3783..7181,7182..7184,7185..7781,7782,7783..7785,
                     7786..7787,7788..7790,7791..7883)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3783..3806,3807..3809,3810..7883)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7896..8144
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8157..8177
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8186..8203
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        8242..8742
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8277..8300)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8675..8694
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8763..9362
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8763..8782)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9259..9279
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9415..10003
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9468..9488)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9886..9897)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10006..10022)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10007..10023)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10532..10712
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10774..11362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10854..10873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11533..12324,12325..12393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12156..12175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12394..12498)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
       61 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
      121 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
      181 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      241 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      301 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      361 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      421 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      481 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      541 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      601 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      661 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      721 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      781 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
      841 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      901 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      961 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     1021 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     1081 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     1141 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     1201 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     1261 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     1321 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1381 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1441 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1501 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     1561 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1621 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1681 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1741 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1801 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1861 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1921 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1981 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     2041 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     2101 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     2161 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     2221 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     2281 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2341 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2401 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     2461 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2521 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2581 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc gattctagat
     2641 tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     2701 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     2761 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     2821 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     2881 agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     2941 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt
     3001 accacttcct accctcgtaa agtcgagctt gcgttggatc cgccaccatg agctcagaga
     3061 ctggcccagt ggctgtggac cccacattga gacggcggat cgagccccat gagtttgagg
     3121 tattcttcga tccgagagag ctccgcaagg agacctgcct gctttacgaa attaattggg
     3181 ggggccggca ctccatttgg cgacatacat cacagaacac taacaagcac gtcgaagtca
     3241 acttcatcga gaagttcacg acagaaagat atttctgtcc gaacacaagg tgcagtatta
     3301 cctggtttct cagctggagc ccatgcggcg aatgtagtag ggccatcact gaattcctgt
     3361 caaggtatcc ccacgtcact ctgtttattt acatcgcaag gctgtaccac cacgctgacc
     3421 cccgcaatcg acaaggcctg cgggatttga tctcttcagg tgtgactatc caaattatga
     3481 ctgagcagga gtcaggatac tgctggagaa actttgtgaa ttatagcccg agtaatgaag
     3541 cccactggcc taggtatccc catctgtggg tacgactgta cgttcttgaa ctgtactgca
     3601 tcatactggg cctgcctcct tgtctcaaca ttctgagaag gaagcagcca cagctgacat
     3661 tctttaccat cgctcttcag tcttgtcatt accagcgact gcccccacac attctctggg
     3721 ccaccgggtt gaaaagcggc agcgagactc ccgggacctc agagtccgcc acacccgaaa
     3781 gtgataaaaa gtattctatt ggtttagcca tcggcactaa ttccgttgga tgggctgtca
     3841 taaccgatga atacaaagta ccttcaaaga aatttaaggt gttggggaac acagaccgtc
     3901 attcgattaa aaagaatctt atcggtgccc tcctattcga tagtggcgaa acggcagagg
     3961 cgactcgcct gaaacgaacc gctcggagaa ggtatacacg tcgcaagaac cgaatatgtt
     4021 acttacaaga aatttttagc aatgagatgg ccaaagttga cgattctttc tttcaccgtt
     4081 tggaagagtc cttccttgtc gaagaggaca agaaacatga acggcacccc atctttggaa
     4141 acatagtaga tgaggtggca tatcatgaaa agtacccaac gatttatcac ctcagaaaaa
     4201 agctagttga ctcaactgat aaagcggacc tgaggttaat ctacttggct cttgcccata
     4261 tgataaagtt ccgtgggcac tttctcattg agggtgatct aaatccggac aactcggatg
     4321 tcgacaaact gttcatccag ttagtacaaa cctataatca gttgtttgaa gagaacccta
     4381 taaatgcaag tggcgtggat gcgaaggcta ttcttagcgc ccgcctctct aaatcccgac
     4441 ggctagaaaa cctgatcgca caattacccg gagagaagaa aaatgggttg ttcggtaacc
     4501 ttatagcgct ctcactaggc ctgacaccaa attttaagtc gaacttcgac ttagctgaag
     4561 atgccaaatt gcagcttagt aaggacacgt acgatgacga tctcgacaat ctactggcac
     4621 aaattggaga tcagtatgcg gacttatttt tggctgccaa aaaccttagc gatgcaatcc
     4681 tcctatctga catactgaga gttaatactg agattaccaa ggcgccgtta tccgcttcaa
     4741 tgatcaaaag gtacgatgaa catcaccaag acttgacact tctcaaggcc ctagtccgtc
     4801 agcaactgcc tgagaaatat aaggaaatat tctttgatca gtcgaaaaac gggtacgcag
     4861 gttatattga cggcggagcg agtcaagagg aattctacaa gtttatcaaa cccatattag
     4921 agaagatgga tgggacggaa gagttgcttg taaaactcaa tcgcgaagat ctactgcgaa
     4981 agcagcggac tttcgacaac ggtagcattc cacatcaaat ccacttaggc gaattgcatg
     5041 ctatacttag aaggcaggag gatttttatc cgttcctcaa agacaatcgt gaaaagattg
     5101 agaaaatcct aacctttcgc ataccttact atgtgggacc cctggcccga gggaactctc
     5161 ggttcgcatg gatgacaaga aagtccgaag aaacgattac tccatggaat tttgaggaag
     5221 ttgtcgataa aggtgcgtca gctcaatcgt tcatcgagag gatgaccaac tttgacaaga
     5281 atttaccgaa cgaaaaagta ttgcctaagc acagtttact ttacgagtat ttcacagtgt
     5341 acaatgaact cacgaaagtt aagtatgtca ctgagggcat gcgtaaaccc gcctttctaa
     5401 gcggagaaca gaagaaagca atagtagatc tgttattcaa gaccaaccgc aaagtgacag
     5461 ttaagcaatt gaaagaggac tactttaaga aaattgaatg cttcgattct gtcgagatct
     5521 ccggggtaga agatcgattt aatgcgtcac ttggtacgta tcatgacctc ctaaagataa
     5581 ttaaagataa ggacttcctg gataacgaag agaatgaaga tatcttagaa gatatagtgt
     5641 tgactcttac cctctttgaa gatcgggaaa tgattgagga aagactaaaa acatacgctc
     5701 acctgttcga cgataaggtt atgaaacagt taaagaggcg tcgctatacg ggctggggac
     5761 gattgtcgcg gaaacttatc aacgggataa gagacaagca aagtggtaaa actattctcg
     5821 attttctaaa gagcgacggc ttcgccaata ggaactttat gcagctgatc catgatgact
     5881 ctttaacctt caaagaggat atacaaaagg cacaggtttc cggacaaggg gactcattgc
     5941 acgaacatat tgcgaatctt gctggttcgc cagccatcaa aaagggcata ctccagacag
     6001 tcaaagtagt ggatgagcta gttaaggtca tgggacgtca caaaccggaa aacattgtaa
     6061 tcgagatggc acgcgaaaat caaacgactc agaaggggca aaaaaacagt cgagagcgga
     6121 tgaagagaat agaagagggt attaaagaac tgggcagcca gatcttaaag gagcatcctg
     6181 tggaaaatac ccaattgcag aacgagaaac tttacctcta ttacctacaa aatggaaggg
     6241 acatgtatgt tgatcaggaa ctggacataa accgtttatc tgattacgac gtcgatcaca
     6301 ttgtacccca atcctttttg aaggacgatt caatcgacaa taaagtgctt acacgctcgg
     6361 ataagaaccg agggaaaagt gacaatgttc caagcgagga agtcgtaaag aaaatgaaga
     6421 actattggcg gcagctccta aatgcgaaac tgataacgca aagaaagttc gataacttaa
     6481 ctaaagctga gaggggtggc ttgtctgaac ttgacaaggc cggatttatt aaacgtcagc
     6541 tcgtggaaac ccgccaaatc acaaagcatg ttgcacagat actagattcc cgaatgaata
     6601 cgaaatacga cgagaacgat aagctgattc gggaagtcaa agtaatcact ttaaagtcaa
     6661 aattggtgtc ggacttcaga aaggattttc aattctataa agttagggag ataaataact
     6721 accaccatgc gcacgacgct tatcttaatg ccgtcgtagg gaccgcactc attaagaaat
     6781 acccgaagct agaaagtgag tttgtgtatg gtgattacaa agtttatgac gtccgtaaga
     6841 tgatcgcgaa aagcgaacag gagataggca aggctacagc caaatacttc ttttattcta
     6901 acattatgaa tttctttaag acggaaatca ctctggcaaa cggagagata cgcaaacgac
     6961 ctttaattga aaccaatggg gagacaggtg aaatcgtatg ggataagggc cgggacttcg
     7021 cgacggtgag aaaagttttg tccatgcccc aagtcaacat agtaaagaaa actgaggtgc
     7081 agaccggagg gttttcaaag gaatcgattc ttccaaaaag gaatagtgat aagctcatcg
     7141 ctcgtaaaaa ggactgggac ccgaaaaagt acggtggctt cgatagccct acagttgcct
     7201 attctgtcct agtagtggca aaagttgaga agggaaaatc caagaaactg aagtcagtca
     7261 aagaattatt ggggataacg attatggagc gctcgtcttt tgaaaagaac cccatcgact
     7321 tccttgaggc gaaaggttac aaggaagtaa aaaaggatct cataattaaa ctaccaaagt
     7381 atagtctgtt tgagttagaa aatggccgaa aacggatgtt ggctagcgcc ggagagcttc
     7441 aaaaggggaa cgaactcgca ctaccgtcta aatacgtgaa tttcctgtat ttagcgtccc
     7501 attacgagaa gttgaaaggt tcacctgaag ataacgaaca gaagcaactt tttgttgagc
     7561 agcacaaaca ttatctcgac gaaatcatag agcaaatttc ggaattcagt aagagagtca
     7621 tcctagctga tgccaatctg gacaaagtat taagcgcata caacaagcac agggataaac
     7681 ccatacgtga gcaggcggaa aatattatcc atttgtttac tcttaccaac ctcggcgctc
     7741 cagccgcatt caagtatttt gacacaacga tagatcgcaa acgatacact tctaccaagg
     7801 aggtgctaga cgcgacactg attcaccaat ccatcacggg attatatgaa actcggatag
     7861 atttgtcaca gcttgggggt gactctggtg gttctactaa tctgtcagat attattgaaa
     7921 aggagaccgg taagcaactg gttatccagg aatccatcct catgctccca gaggaggtgg
     7981 aagaagtcat tgggaacaag ccggaaagcg atatactcgt gcacaccgcc tacgacgaga
     8041 gcaccgacga gaatgtcatg cttctgacta gcgacgcccc tgaatacaag ccttgggctc
     8101 tggtcataca ggatagcaac ggtgagaaca agattaagat gctctctggt ggttctccca
     8161 agaagaagag gaaagtctaa ccggtcatca tcaccatcac cattgaaaac cgctgttcct
     8221 aggaatcccg aggcctctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     8281 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     8341 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     8401 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     8461 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     8521 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     8581 gggctcagag gctgggaagg ggtgggtccg gggggcgggc tcaggggcgg gctcaggggc
     8641 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     8701 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
     8761 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     8821 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     8881 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     8941 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     9001 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     9061 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     9121 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     9181 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     9241 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     9301 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     9361 gaaaacgttg ttgtttgggg ttgaattact cttctagacc gcggcgcgcc cgataatcaa
     9421 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9481 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9541 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9601 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9661 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9721 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     9781 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     9841 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9901 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9961 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
    10021 cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
    10081 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
    10141 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
    10201 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
    10261 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
    10321 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
    10381 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
    10441 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
    10501 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
    10561 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    10621 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10681 acccttttag tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc
    10741 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10801 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10861 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10921 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10981 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    11041 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    11101 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    11161 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    11221 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    11281 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    11341 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11401 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    11461 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    11521 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    11581 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    11641 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    11701 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    11761 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    11821 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    11881 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    11941 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    12001 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    12061 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    12121 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    12181 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    12241 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    12301 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    12361 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    12421 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    12481 acaaataggg gttccgcgca cattt
//
