LOCUS       sequence-22008-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Liu JK, Ghattas I, Liu S, Chen S, Rubenstein JL
  TITLE     Dlx genes encode DNA-binding proteins that are expressed in an 
            overlapping and sequential pattern during basal ganglia 
            differentiation.
  JOURNAL   Dev Dyn. 1997 Dec . 210(4):498-512.
  PUBMED    9415433
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            1997 Dec . 210(4):498-512."
COMMENT     Sequence Label: Dlx6
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(255..271)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(256..272)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(300..318)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(339..355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(339..355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(352..374)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    363..379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(387..393,394..411,412..417))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    432..453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(570..587)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagagtcctg gatcctcctt gcctccttca gaagctccgt aggcgttcac tcagagaagt
       61 cacatcatct gtggtctctg catggtgtcc tggtgtggtg aggaatacca gtgtgaatac
      121 cccggcatgt agctgttggg aggcatactg acgcccttgg cagaggcaga aacgtcccac
      181 actggaggca gggctggtga tcgtggtgac agggctgctg aacccgggct gcaggaattc
      241 gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccag cttttgttcc
      301 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
      361 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      421 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      481 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      541 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      601 cggctgcggc gagcggtat
//
