LOCUS       sequence-220087-        5653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conditional ablation of mature bone cells via ganciclovir exposure 
            of cells expressing a truncated thymidine kinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Visnjic D, Kalajzic I, Gronowicz G, Aguila HL, Clark SH, Lichtler 
            AC, Rowe DW
  TITLE     Conditional ablation of the osteoblast lineage in Col2.3deltatk 
            transgenic mice.
  JOURNAL   J Bone Miner Res. 2001 Dec;16(12):2222-31. doi: 
            10.1359/jbmr.2001.16.12.2222.
  PUBMED    11760835
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1359/jbmr.2001.16.12"; journalName: "J Bone Miner Res"; date: 
            "2001-12"; volume: "16"; issue: "12"; pages: "2222-31"
COMMENT     Sequence Label: Col2.3deltaTK
FEATURES             Location/Qualifiers
     source          1..5653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2434..3429
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(3427..3446)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3527..3751
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3813..3831)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3899..4003
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4004..4072,4073..4864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4222..4241)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5035..5623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5524..5543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcgataagc ttggaaccca ctgcccacaa ctcctggggc aaaaacctgg agtcagacat
       61 ggggtgaagg ctgtcacacg gcacagacac gtcaagcacc ccccccaatt ctagtagtct
      121 cctagcctcc accagaaccc cagacccttg atgtggcagt caccagtcca cacctgttag
      181 gctcttgtct cttcttccag atgagcctgg ggggcggggg ggtgctagat caggagcagg
      241 gaaaagtagc tttggataag tgcttttccc aatacaaaac ccaacaaaga gtgggcagat
      301 cacactgtgt agtgcttcgt ggaaccctac cctagacaac tgccttgaac acctattccc
      361 tctgatgtac accatccccg tccactgtta gggagtgggc atcctttgga actgaccact
      421 gtggaaggca ggactttact gagttccgga actaccatct cagcttctca gccccagcct
      481 taccctacag gcactggcat aggcgggggc agatcctggg ccacaagtca ctgccacatg
      541 gttgggataa ttgatgaagt cctgtccttc cattgctgtc tccagttctg cttctctgga
      601 aactctatat ttttcccttt aattatagcc tctgcagtct ccctctgcca ccccacccgc
      661 accgcttagc ctaactgccc acggccagcg acgtggctcc ctccccttct gctcccttgg
      721 tcttttttat ttttttttct ttgccttcgt tgcacaaaac tagctcaggg agggcgtgaa
      781 ggggggggga gcaatggaat cttggatggt ttggaggagg cgggactcct tgcttccacg
      841 tttacagctc tgaagacggc tgtgggggaa gtgatacagg acgtctatgg gccctgagag
      901 gagaccccta tgcttccctg ccacccacac agtttaacaa aatgaagttc ctaagtagag
      961 tgggggtcag gcagagcacc tttgcagggt tgatgggagc ccagggaaag aaaggacact
     1021 gtcttttagg gacacattta aatataagcc acttttcttg ggggacgaca aatgaccctt
     1081 tcctgattgc agaggtgggg aacaatggct gagattttca gcaaagaagc gaggacatga
     1141 ggagtagcct tcaaataaag tcactcagct accaaaaaca agtttctgcc acacaccgag
     1201 ttcacctagg tgtccccaga ccagatccaa gtacagtaag gaaagcaggt tctctacaga
     1261 gagaacacgg ctctatggcc aatgccttct acctgctctt tctggattga tactgctacc
     1321 taagagggcc tctaaccaat tcctggctgt agccacagct gacacaagac ctttttctaa
     1381 gacatccctg gtcacaggcc tcctgtagca aattccagcc ctgggatgga ggtggtcagg
     1441 aaagagttta tacaagaaga cccaggccac agctttaagg actcagaaac ccccctgccc
     1501 acacggctgc ccatcataac gcagaaggtt tcttctggaa ggacaaggat gtcaaacttc
     1561 tccccaagcc taatcctcag agatgtctcc ctctgttaca cctggggctg gagaaaggtg
     1621 ggtctttcat ggagccacat tcatggcaga acagatagcc accccactcc tttcaaacaa
     1681 ccacatatct gactcttagt atctgtgaag agatgtctaa tttgttccca aatattccta
     1741 ccctgcatac ctgggcccac accatgaggt attctcctcc ctctaacagt cacatctgct
     1801 tagctgcctg gttcttcgga tttggagaga tgcttgccta acttattctt ccttaggtct
     1861 tcccaaggat gccagaaaga ctatgagaca tggccaagag gaccttttcc caattgtgcc
     1921 tgacactgaa ccctttgtaa tgttccccaa ctcagattcc caattctaca tccttctgat
     1981 ttgaggtccc agaaggaaag tgcaaggggc atcccctacc cacaatcagt atatcgaggg
     2041 cccagccaca ctcagtgata gcacctctgg cccatgtaga tctgggggac aagggtggca
     2101 gaattgcaaa ggggggaggg ggctgggtgg actcctttcc cttcctttcc ctcctccccc
     2161 ctcttcgttc caaattgggg gccgggccag gcagttctga ttggctgggg gccgggctgc
     2221 tggctccccc tctccaagag gcagggttcc tcccagccct cctccatcag gatggtataa
     2281 aaggggccca ggccagtcgt cggagcagac gggagtttca cctccggacg gagcaggagg
     2341 cacacggagt gaggccacgc atgagccgaa gctaaccccc caccccagcc gcaaagagtc
     2401 tacatgtcta gggtctagag gatccgcgag cttatgccca cgctactgcg cgtttatata
     2461 gacggtcctc acgggatggg gaaaaccacc accacgcaac tgctggtggc cctgggttcg
     2521 cgcgacgata tcgtctacgt acccgagccg atgacttact ggcaggtgct gggggcttcc
     2581 gagacaatcg cgaacatcta caccacacaa caccgcctcg accagggtga gatatcggcc
     2641 ggggacgcgg cggtggtaat gacaagcgcc cagataacaa tgggcatgcc ttatgccgtg
     2701 accgacgccg ttctggctcc tcatatcggg ggggaggctg ggagctcaca tgccccgccc
     2761 ccggccctca ccctcatctt cgaccgccat cccatcgccg ccctcctgtg ctacccggcc
     2821 gcgcgatacc ttatgggcag catgaccccc caggccgtgc tggcgttcgt ggccctcatc
     2881 ccgccgacct tgcccggcac aaacatcgtg ttgggggccc ttccggagga cagacacatc
     2941 gaccgcctgg ccaaacgcca gcgccccggc gagcggcttg acctggctat gctggccgcg
     3001 attcgccgcg tttacgggct gcttgccaat acggtgcggt atctgcaggg cggcgggtcg
     3061 tggcgggagg attggggaca gctttcgggg acggccgtgc cgccccaggg tgccgagccc
     3121 cagagcaacg cgggcccacg accccatatc ggggacacgt tatttaccct gtttcgggcc
     3181 cccgagttgc tggcccccaa cggcgacctg tacaacgtgt ttgcctgggc cttggacgtc
     3241 ttggccaaac gcctccgtcc catgcacgtc tttatcctgg attacgacca atcgcccgcc
     3301 ggctgccggg acgccctgct gcaacttacc tccgggatgg tccagaccca cgtcaccacc
     3361 cccggctcca taccgacgat ctgcgacctg gcgcgcacgt ttgcccggga gatgggggag
     3421 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     3481 aggacagaat aaaacgcacg ggtggatccc cgggcgagct cgaattctgt gccttctagt
     3541 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3601 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3661 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3721 aggcatgctg gggatgcggt gggctctatg gaattccgag cttatcgatg ataagctgtc
     3781 aaacatgaga attaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     3841 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3901 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3961 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4021 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4081 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4141 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4201 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     4261 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4321 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4381 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4441 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4501 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     4561 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4621 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4681 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4741 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4801 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4861 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4921 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4981 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5161 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     5221 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5281 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5341 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5401 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5641 ccgaagctag ctt
//
