LOCUS       sequence-220102-        8171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Iwamoto M, Bjoerklund T, Lundberg C, Kirik D, Wandless TJ
  TITLE     A general chemical method to regulate protein stability in the 
            mammalian central nervous system.
  JOURNAL   Chem Biol. 2010 Sep 24. 17(9):981-8.
  PUBMED    20851347
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem Biol. 
            2010 Sep 24. 17(9):981-8."
COMMENT     Sequence Label: pBMN YFP-DHFR(DD)
FEATURES             Location/Qualifiers
     source          1..8171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..892
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             957..1373
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1303..1325
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1341..1357
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1383..1757
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1695..1711
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             1775..1801
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1802..1804,1805..1807,1808..2518)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1847..1868)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2108..2127)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2455..2476
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2525..3001
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     misc_feature    join(3033..3607,3608..3610,3611..3619)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3200..3217)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3427..3446
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3620..4303
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     CDS             4316..5002
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     LTR             5173..5642
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5772..5789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5943..6531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6023..6042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6695..7486,7487..7555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7318..7337
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      721 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      781 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      841 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
      901 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
      961 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1021 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1081 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1141 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1201 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1261 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1321 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1381 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1441 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     1501 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
     1561 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
     1621 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
     1681 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
     1741 gaccatcctc tagactgccg gatccgccac catgtatccg tacgacgtac cggattatgc
     1801 gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1861 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1921 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1981 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
     2041 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     2101 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2161 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2221 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2281 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2341 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2401 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2461 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagct
     2521 cgagatcagt ctgattgcgg cgttagcggt agatcacgtt atcggcatgg aaaccgtcat
     2581 gccgtggaac ctgcctgccg atctcgcctg gtttaaacgc aacaccttaa ataaacccgt
     2641 gattatgggc cgccatacct gggaatcaat cggtcgtccg ttgccaggac gcaaaaatat
     2701 tatcctcagc agtcaaccga gtacggacga tcgcgtaacg tgggtgaagt cggtggatga
     2761 agccatcgcg gcgtgtggtg acgtaccaga aatcatggtt attggcggcg gtcgcgttta
     2821 tgaacagttc ttgccaaaag cgcaaaaact gtatctgacg catatcgacg cagaagtgga
     2881 aggcgacacc catttcccgg attacgagcc ggatgactgg gaatcggtat tcagcgaatt
     2941 ccacgatgct gatgcgcaga actctcacag ctattgcttt gagattctgg agcggcgatg
     3001 accgcggccg ctacgtaaat tccgcccccc cccccctctc cctccccccc ccctaacgtt
     3061 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     3121 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     3181 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     3241 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     3301 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     3361 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     3421 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     3481 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     3541 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     3601 tgataatatg gccacaacca tggtgagcgg cctgctgaag gagagtatgc gcatcaagat
     3661 gtacatggag ggcaccgtga acggccacta cttcaagtgc gagggcgagg gcgacggcaa
     3721 ccccttcgcc ggcacccaga gcatgagaat ccacgtgacc gagggcgccc ccctgccctt
     3781 cgccttcgac atcctggccc cctgctgcga gtacggcagc aggaccttcg tgcaccacac
     3841 cgccgagatc cccgacttct tcaagcagag cttccccgag ggcttcacct gggagagaac
     3901 caccacctac gaggacggcg gcatcctgac cgcccaccag gacaccagcc tggagggcaa
     3961 ctgcctgatc tacaaggtga aggtgcacgg caccaacttc cccgccgacg gccccgtgat
     4021 gaagaacaag agcggcggct gggagcccag caccgaggtg gtgtaccccg agaacggcgt
     4081 gctgtgcggc cggaacgtga tggccctgaa ggtgggcgac cggcacctga tctgccacca
     4141 ctacaccagc taccggagca agaaggccgt gcgcgccctg accatgcccg gcttccactt
     4201 caccgacatc cggctccaga tgctgcggaa gaagaaggac gagtacttcg agctgtacga
     4261 ggccagcgtg gcccggtaca gcgacctgcc cgagaaggcc aacagatctc ccgggatggt
     4321 gagcggcctg ctgaaggaga gtatgcgcat caagatgtac atggagggca ccgtgaacgg
     4381 ccactacttc aagtgcgagg gcgagggcga cggcaacccc ttcgccggca cccagagcat
     4441 gagaatccac gtgaccgagg gcgcccccct gcccttcgcc ttcgacatcc tggccccctg
     4501 ctgcgagtac ggcagcagga ccttcgtgca ccacaccgcc gagatccccg acttcttcaa
     4561 gcagagcttc cccgagggct tcacctggga gagaaccacc acctacgagg acggcggcat
     4621 cctgaccgcc caccaggaca ccagcctgga gggcaactgc ctgatctaca aggtgaaggt
     4681 gcacggcacc aacttccccg ccgacggccc cgtgatgaag aacaagagcg gcggctggga
     4741 gcccagcacc gaggtggtgt accccgagaa cggcgtgctg tgcggccgga acgtgatggc
     4801 cctgaaggtg ggcgaccggc acctgatctg ccaccactac accagctacc ggagcaagaa
     4861 ggccgtgcgc gccctgacca tgcccggctt ccacttcacc gacatccggc tccagatgct
     4921 gcggaagaag aaggacgagt acttcgagct gtacgaggcc agcgtggccc ggtacagcga
     4981 cctgcccgag aaggccaact gagtcgacga taaaataaaa gattttattt agtctccaga
     5041 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat
     5101 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     5161 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     5221 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     5281 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc
     5341 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     5401 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     5461 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     5521 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     5581 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     5641 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg
     5701 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5761 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5821 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5881 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5941 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6001 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6061 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6121 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6181 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6241 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6301 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6361 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6421 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6481 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6541 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6601 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccggc cgcaaatcaa
     6661 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6721 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6781 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6841 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6901 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6961 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7021 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7081 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7141 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7201 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7261 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7321 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7381 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7441 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7501 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7561 tcctttttca atattattga agcatttatc agggttattg tctcatgacc aaaatccctt
     7621 aacgtgagtt ttcgttccat ttccccgaaa agtgccacca gctttgctct taggagtttc
     7681 ctaatacatc ccaaactcaa atatataaag catttgactt gttctatgcc ctagggggcg
     7741 gggggaagct aagccagctt tttttaacat ttaaaatgtt aattccattt taaatgcaca
     7801 gatgttttta tttcataagg gtttcaatgt gcatgaatgc tgcaatattc ctgttaccaa
     7861 agctagtata aataaaaata gataaacgtg gaaattactt agagtttctg tcattaacgt
     7921 ttccttcctc agttgacaac ataaatgcgc tgctgagaag ccagtttgca tctgtcagga
     7981 tcaatttccc attatgccag tcatattaat tactagtcaa ttagttgatt tttatttttg
     8041 acatatacat gtgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
     8101 ttgcaaggca tggaaaaata cataactgag aatagaaaag ttcagatcaa ggtcaggaac
     8161 agatggaaca g
//
