LOCUS       sequence-22012-e         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Bulfone A, Smiga SM, Shimamura K, Peterson A, Puelles L, Rubenstein 
            JL
  TITLE     T-brain-1: a homolog of Brachyury whose expression defines 
            molecularly distinct domains within the cerebral cortex.
  JOURNAL   Neuron. 1995 Jul . 15(1):63-78.
  PUBMED    7619531
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            1995 Jul . 15(1):63-78."
COMMENT     Sequence Label: Tbr1
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(320..338)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(359..375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(359..375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(372..394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    383..399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(407..413,414..431,432..437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    452..473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(590..607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tggtgaaata tgcatcatac acaatattca atctagattc cagtctatgg ggggatttgt
       61 cctaatagga attcagggtc taaacgtggg tatactttgg ctctcctgta aatcaaatgt
      121 tgtgattttt tatatttgtt ttgttttgtc tgtgaattga ataatttata caagtacaca
      181 ctccactgag aatcgttttg ttttctgctc gtttgtatcg tctgtgtata acaagtaaaa
      241 taaatctggt aaaatgctaa aaaaaaaaaa aaagcggccg cggaggcctc tagacctcga
      301 ggagctccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt
      361 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      421 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      481 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      541 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      601 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      661 ta
//
