LOCUS       sequence-220126-        8435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ARRET expression contains approximately 130 anti-sense telomere 
            hexanucleotide repeats of TTAGGG and TTGGGG variant sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8435)
  TITLE     telomeric repeat-containing RNA (TERRA and ARRET) plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV UbC6-ARRET
FEATURES             Location/Qualifiers
     source          1..8435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1803..3014
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2851..2870
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3074..3098
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3868..3892)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3945..3986
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4023..4043)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4028..4357
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4208..4343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4405..4452
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4471..4869
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4956..5189
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5261..5382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5298..5317)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5352..5371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5422..5557
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5577..5596)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5578..5596)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5606..5623)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5606..5622)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5615..5637)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5764..6219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5851..5870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6061..6082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6245..6349
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6350..6418,6419..7210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6568..6587)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7381..7969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7870..7889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8123..8140
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8257..8278
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8293..8298,8299..8316,8317..8323)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8331..8347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8336..8358
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8355..8371
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8355..8371
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8390..8410
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8392..8410
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggat
     1801 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     1861 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     1921 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     1981 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     2041 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     2101 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     2161 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     2221 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     2281 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     2341 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     2401 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     2461 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     2521 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     2581 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     2641 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     2701 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     2761 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     2821 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     2881 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     2941 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     3001 cttttttgtt agacgaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat
     3061 tctgcagata tcaacaagtt tgtacaaaaa agcaggcttt aaaggaacca attcagtcga
     3121 tcgactggat cgatgaattc cgaaccctaa ccctaaccct aaccctaacc ctaaccctaa
     3181 ccctaaccct aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa
     3241 ccctaaccct aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccca
     3301 ccccaacccc aaccccaacc ccacacccca ccccaaaccc ccaccccaac cccaacccca
     3361 accccaaccc caaccccaac cccaacccca accctaaccc acacccccac ccacacccca
     3421 accccaaccc ccacccccac ccccaccccc aaccacacca caacaccaac mcccacacca
     3481 ccccgacccc caccccaccc cctaccccag acctcaccca cacccacacc ctaaccccac
     3541 ccccaaccct aaccccaacc ctaaccccaa ccctaaccct aaccctaacc ctaaccctaa
     3601 ccctaacccc aaccccaacc ccaaccctaa ccccaacccc aaccccaacc ccaaccccaa
     3661 ccccaacccc aaccccaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccccaa
     3721 ccctaaccct aaccctaacc ctaaccctaa ccccaaccct aaccccaacc ccaaccccaa
     3781 ccccaacccc aaccggaatt ccgcggaatt cgagctcggt acccggggat cgacaattcc
     3841 acttttttta attctcgaga tatctagacc cagctttctt gtacaaagtg gttgatatcc
     3901 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
     3961 accctctcct cggtctcgat tctacgcgta ccggttagta atgagtttgg aattaattct
     4021 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4081 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4141 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4201 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4261 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4321 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4381 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     4441 gtataatacg acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc
     4501 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     4561 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     4621 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     4681 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     4741 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     4801 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     4861 gagggctaag cacaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     4921 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     4981 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5041 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5101 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5161 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     5221 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     5281 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5341 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     5401 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5461 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5521 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     5581 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5641 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5701 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5761 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5821 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5881 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5941 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6001 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6061 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6121 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6181 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     6241 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6301 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6361 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6421 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6481 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6541 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6601 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6661 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6721 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6781 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6841 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6901 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6961 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7021 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7081 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7141 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7201 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7261 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7321 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7381 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7441 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7501 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7561 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7621 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7681 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7741 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7801 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7861 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7921 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7981 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8041 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8101 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8161 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8221 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8281 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8341 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     8401 tcactaaagg gaacaaaagc tggagctgca agctt
//
