LOCUS       sequence-220131-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of firefly luciferase in T. gondii using the
            alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT
            locus flanking sequence to direct integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  TITLE     T. gondii pTUB:FLUC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTUB:FLuc
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..38)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(21..37)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(30..52)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(425..443)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        511..615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(616..684,685..1476)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(834..853)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1647..2235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2136..2155
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2362..2384
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2381..2397
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2381..2397
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2417..2437
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(3211..3244)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6076..6109)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7821..7839)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(join(10783..10790,1..13))
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
       61 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      121 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      181 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
      241 ctcagtgcaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
      301 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
      361 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
      421 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      481 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      541 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      601 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      661 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      721 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      781 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      841 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      901 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      961 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1021 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1081 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1141 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1201 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     1261 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     1321 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggt cagatggtaa gccctcccgt
     1381 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     1441 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1501 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1561 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1621 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1681 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1741 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1801 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1861 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1921 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1981 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2041 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2101 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2161 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2221 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     2281 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2341 gcctttgagt gagctgatac cagcggataa caatttcaca caggaaacag ctatgaccat
     2401 gattacgcca agctatcaac tttgtataga aaagttgagc tactcctgtg tctgagtggg
     2461 aattaggtct cccgtatgtt ttccatctct gccccgtcta tatcccctgt ttgtctcctt
     2521 cagtcttcta cgccgaccgc ctgattcgcc tcctcatcga agaagctttg aacggtgagt
     2581 ctcaagtctc aaaagcagat ccgcttctct tgtactgcta gcatgagtct gaaaatgtag
     2641 gcaacgccta aaaagcggtg catgacccac ttcagtctac gtcaaaaaca cgccgctttt
     2701 ttggagagat ggagttaatc ggtttgctga gaaactgtac aatttattta attcgagact
     2761 tggattgttt gacatggcag gaagggtctg agcgtgcagc tgatgcaggc agactctagt
     2821 gagtgtccca acgtcgcaag taactgctgg aagcatgcag ccatatacga aactccgagg
     2881 agtaaattgt gttcttctgt cggtgtctca catgtgtttt ctgtttcgag ggtcaacatg
     2941 tttacatatg tagatatgag tttgaacgcg tgtatcccgc ttcatacgta cgtctgtctc
     3001 tgattgcctt cttcacttct tcatgtctat caacatgtta ctgcgtttgc ggatgcggag
     3061 ttctagactt tcaactgacg tcgggtgcct acgttcttct acattttact gcagaactgc
     3121 cgttcgaaaa gaaggaagtg acaacccctc tggatgtgtc ataccatgga gtttccttct
     3181 attccaagat cgaattcgag ctcggtaccc ataacttcgt atagcataca ttatacgaag
     3241 ttataagctt cgccaggctg taaatcccgt gagtcgtcct cacaaatcat caagcaggtg
     3301 tcctcaggga gactgcctga ctgagttatg ctaattcctt tctactttgg cgtggtcacg
     3361 ggcgcgccgg atccttaatt aagtctagca tgtcattcga ttttcacccc ccgcgtagtt
     3421 cctgtgtgtc attcgttgtc gagacaactc tgtcccgccc cggtgctgtt ccatatgcgt
     3481 gactttcccg caattttttc agactttcag gaaagacagg ctccggaacg atctcgtcca
     3541 tgactggtaa atccacgaca ccgcaatggc ccccagcacc tctatctctc gtgccagggg
     3601 actaacgttg tatgcgtctg cgtcttgtct ttttgcattc gctttccaaa aaagagagcc
     3661 atccgttccc ccgcacattc aacgccgcga gtgcggtttt tgtctttttt gagtggtagg
     3721 acgcttttca tgcgcgaact acgtggacat taagttccat tctctttttc gacagcacga
     3781 aaccttgcat tcaaacccgc ccgcggaaga tccgatcttg ctgctgttcg cagtcccagt
     3841 agcgtcctgt cggccgcgcc gtctctgttg gtgggcagcc gctacacctg ttatctgact
     3901 gccgtgcgcg aaaatgacgc catttttggg aaaatcgggg aacttcattc tttaaaagta
     3961 tgcggaggtt tcctttttct tctgttcgtt tctttttctc gggtttgata accgtgttcg
     4021 atgtaagcac tttccgtctc tcctccgtgc tttgttcgac atcgagacca ggtgtgcaga
     4081 tccttcgctt gtcgatccgg agacgcgtgt ctcgtagaac cttttcattt taccacacgg
     4141 cagtgcggag cactgctctg agtgcagcag ggacgggtga agtttcgctt tagtagtgcg
     4201 tttctgctct acggggcgtt gtcgtgtctg ggaagatgca gaaaccggtg tgtctggtcg
     4261 tcgcgatgac ccccaagagg ggcatcggca tcaacaacgg cctcccgtgg ccccacttga
     4321 ccacagattt caaacacttt cgtcgtgtga caaaaacgac gcccgaagaa gccagtcgcc
     4381 tgaacgggtg gcttcccagg aaatttgcaa agacgggcga ctctggactt ccctctccat
     4441 cagtcggcaa gagattcaac gccgttgtca tgggacggaa aaactgggaa agcatgcctc
     4501 gaaagtttag acccctcgtg gacagattga acatcgtcgt ttcctcttcc ctcaaagaag
     4561 aagacattgc ggcggagaag cctcaagctg aaggccagca gcgcgtccga gtctgtgctt
     4621 cactcccagc agctctcagc cttctggagg aagagtacaa ggattctgtc gaccagattt
     4681 ttgtcgtggg aggagcggga ctgtacgagg cagcgctgtc tctgggcgtt gcctctcacc
     4741 tgtacatcac gcgtgtagcc cgcgagtttc cgtgcgacgt tttcttccct gcgttccccg
     4801 gagatgacat tctttcaaac aaatcaactg ctgcgcaggc tgcagctcct gccgagtctg
     4861 tgttcgttcc cttttgtccg gagctcggaa gagagaagga caatgaagcg acgtatcgac
     4921 ccatcttcat ttccaagacc ttctcagaca acggggtacc ctacgacttt gtggttctcg
     4981 agaagagaag gaagactgac gacgcagcca ctgcggaacc gagcaacgca atgagctcct
     5041 tgacgtccac gagggagaca actcccgtgc acgggttgca ggctccttct tcggccgcag
     5101 ccattgcccc ggtgttggcg tggatggacg aagaagaccg gaaaaaacgc gagcaaaagg
     5161 aactgattcg ggccgttccg catgttcact ttagaggcca tgaagaattc cagtaccttg
     5221 atctcattgc cgacattatt aacaatggaa ggacaatgga tgaccgaacg ggcgttggtg
     5281 tcatctccaa attcggctgc actatgcgct actcgctgga tcaggccttt ccacttctca
     5341 ccacaaagcg tgtgttctgg aaaggggtcc tcgaagagtt gctgtggttc attcgcggcg
     5401 acacgaacgc aaaccatctt tctgagaagg gcgtgaagat ctgggacaag aatgtgacac
     5461 gcgagttcct cgattcgcgc aatctccccc accgagaggt cggagacatc ggcccgggct
     5521 acggcttcca gtggagacac ttcggcgcgg catacaaaga catgcacaca gactacacag
     5581 ggcagggcgt cgaccagctg aagaatgtga tccagatgct gagaacgaat ccaacagatc
     5641 gtcgcatgct catgactgcc tggaatcctg cagcgctgga cgaaatggcg ctgccgcctt
     5701 gtcacttgtt gtgccagttc tacgtgaacg accagaagga gctgtcgtgc atcatgtatc
     5761 agcggtcgtg cgatgtcggc ctcggcgtcc ccttcaacat cgcttcctat tcgcttttga
     5821 cgctcatggt tgcacacgtc tgcaacctaa aacctaagga gttcattcac ttcatgggga
     5881 acacgcatgt ctacacgaac catgtcgagg ctttaaaaga gcagctgcgg agagaaccga
     5941 gaccgttccc cattgtgaac atcctcaaca aggaacgcat caaggaaatc gacgatttca
     6001 ccgccgagga ttttgaggtc gtgggctacg tcccgcacgg acgaatccag atggagatgg
     6061 ctgtctaagc tgagcataac ttcgtatagc atacattata cgaagttatc ggaaatacag
     6121 aagctgcccg tctctcgttt tcctctcttt tcggagggat cagggagagt gcctcgggtc
     6181 ggagagagct gacgaggggg tgccagagac ccctgtgtcc tttatcgaag aaaagggatg
     6241 actcttcatg tggcatttca cacagtctca cctcgccttg ttttcttttt gtcaatcaga
     6301 acgaaagcga gttgcgggtg acgcagatgt gcgtgtatcc actcgtgaat gcgttatcgt
     6361 tctgtatgcc gctagagtgc tggactgttg ctgtctgccc acgacagcag acaactttcc
     6421 ttctatgcac ttgcaggatg gtgcagcgca aacgacggag agaaaggagc accctctcag
     6481 tttccctacg atgtgctgtc agtttcgact cttcaccgcg aacgattggc gatacgtctc
     6541 tgttgacttg ttaggctccg accacgaagc tcccttaact aaataagccg cgacacctaa
     6601 gtgtacacca tttgcagatc gataatctgc gaccgctgaa tccgtccaga tcagtaaaac
     6661 cgcaccacct aagtgtaaac cttgtttagg tcgataaaat gctaccaacc cccacccaca
     6721 atcgagcctt gagcgtttct gcgcacgcgt tggcctacgt gacttgctga tgcctgcctc
     6781 tggccattca tgccagtcag tgcgcataaa aatgtggaca cagtcggttg acaagtgttc
     6841 tggcaggcta cagtgacacg gggaaatcgt ttctgagaga gcaccaaaac cttttagctg
     6901 tcaccatctt cttcgcttgc tacgttgtct gatttgtcgt ttttcctctc ggtgtgaagc
     6961 gtttttcgta acgtcaatcg acccaggaca ctgcttccag ctgtctgtgc cgtcctgtcg
     7021 tgaccagttt gtcgccgcga ctttatgagc tttttcctgt tgagaaagcg gttcggcggc
     7081 cgccacgtga catgcgggag aagaaaacgt gaccgaacaa atcaactggt ggagaagagt
     7141 gtgttttcgt ttaattcgtc acagaccacg aaaaacgaac catcgaagac gatcactgcg
     7201 tccgcgtgca tctggatgga tgacccacat ctgttgcagc cgtcgcagac atgcatgtcc
     7261 cgcgttcgtg aaattctctg catcagcgga gtgatcagga atcatcgtct cagcgggatg
     7321 acgttgcgga gcaggccggc tcgcggtggg cagtcagatg ccgaaggcgt aactcaggac
     7381 ggcttgcgct catcgcagaa caggggtggt gcctgcattg ggtgcggttg gtgatcctgg
     7441 ttggaccggt ggagatgcgc gcgcacgaag gggatgtgtc agaaacattt tgtttgttct
     7501 ctgtgaactt ttagatgtgt taaaggcggc gaatattagc agagagtcct ccttgttgga
     7561 ttctctcttg aatttcgccc tttccttctc tttgcgagtc tcgtagagaa caagcactcg
     7621 ttcgccgtcc ctgacgacgc aacccgcgca gaagacatcc accaaacggt gttacacaat
     7681 caccttgtgt gaagttcttg cggaaaacta ctcgttggca ttttttcttg aattcccttt
     7741 tagatctggc aaaaatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     7801 ctctagagga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     7861 ttcctggaac aattgctttt acagatgcac atatcgaggt gaacatcacg tacgcggaat
     7921 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     7981 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     8041 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     8101 acagtatgaa catttcgcag cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa
     8161 ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat tattatcatg gattctaaaa
     8221 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     8281 ttaatgaata cgattttgta ccagagtcct ttgatcgtga caaaacaatt gcactgataa
     8341 tgaattcctc tggatctact gggttaccta agggtgtggc ccttccgcat agaactgcct
     8401 gcgtcagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     8461 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     8521 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttttacgat
     8581 cccttcagga ttacaaaatt caaagtgcgt tgctagtacc aaccctattt tcattcttcg
     8641 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctgggg
     8701 gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa acgcttccat cttccaggga
     8761 tacgacaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     8821 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     8881 tggataccgg gaaaacgctg ggcgttaatc agagaggcga attatgtgtc agaggaccta
     8941 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     9001 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatagttg
     9061 accgcttgaa gtctttaatt aaatacaaag gatatcaggt ggcccccgct gaattggaat
     9121 cgatattgtt acaacacccc aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg
     9181 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     9241 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     9301 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     9361 gagagatcct cataaaggcc aagaagggcg gaaagtaact gcagcccaca gaagctgccc
     9421 gtctctcgtt ttcctctctt ttcggaggga tcagggagag tgcctcgggt cggagagagc
     9481 tgacgagggg gtgccagaga cccctgtgtc ctttatcgaa gaaaagggat gactcttcat
     9541 gtggcatttc acacagtctc acctcgcctt gttttctttt tgtcaatcag aacgaaagcg
     9601 agttgcgggt gacgcagatg tgcgtgtatc cactcgtgaa tgcgttatcg ttctgtatgc
     9661 cgctagagtg ctggactgtt gctgtctgcc cacgacagca gacaactttc cttctatgca
     9721 cttgcaggat ggtgcagcgc aaacgacgga gagaaaggag caccctctca gtttccctac
     9781 gatgtgctgt cagtttcgac tcttcaccgc gaacgattgg cgatacgtct ctgttgactt
     9841 gttaggctcc gaccacgaag ctcccttaac tagataagcc gcgtctagag tcgacctgca
     9901 ggcgcgagtc gatggaaagc ggcttgcggg cagtttgccg cggctgccgc atcgggaaaa
     9961 tcctcatcca gagagacgaa acaactgcgg agcctaaggt acggacttgt ttgcgttctc
    10021 gctacctggg gaccacagac atcaggtgtc ttcgagtgca gctgtcgctg gaatttcccc
    10081 tcctgtcgtt caccgcgaga aaaagctaag gaagagggag aaggtgaaga gcgataggca
    10141 acccaagagg ctgctgtcca ctttgttaca ctaaaacaga ttggagtata agaagcacaa
    10201 cttggcctga aacacagaac agagcggaga gactacgcag ttgtgtctgt ggaggaagag
    10261 cacaattctg ttgtgacggg aaggaagaag agagagggac tcgtaggtca gccggaaggg
    10321 aaggcaggtc gtggaaacta tggaggcaag aggaaagaat tgctgcgctc tgactcactg
    10381 cacgtgtttt ccttacccat tgcctcccat tttctgacac ggatgatatt catcgctgaa
    10441 cttgacagtt ggttgtggct gtttgttgct ggtttgcgtg gccaagaccg ccgtgggcgc
    10501 ccctctgctc ttgtccgctg cacctgcttc ctccgccctt gatgtttgct ctgttccagc
    10561 tgatctacga gaagctgcct gccgacatta gagatcgctg ggtgatgctg ctcgatccga
    10621 tgtgcgcgac ggcgggcagt gtgtgcaaag cgatcgaggt cctcctgagg ctcggcgtga
    10681 aggaagagag aatcattttc gtcaacattt tggctgctcc ccaaggcatt gaacgtgttt
    10741 tcaaggaata cccgaaagtc cgcatggtca ctgctgcgta agcaacttta
//
