LOCUS       sequence-220132-       10023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to Bin1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-Bin1b
FEATURES             Location/Qualifiers
     source          1..10023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1847..1867)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1875..1891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1907..2041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1931..1950)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1985..2004
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2480..2667,2668..2679)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2713..2729)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2713..2729)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2766..2786)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2766..2784)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2805..2821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2818..2840)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2829..2845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2853..2859,2860..2877,2878..2883))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2898..2919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3036..3053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3207..3795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3287..3306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3966..4757,4758..4826))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4589..4608
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4827..4931)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5094..5115)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5464..5475,5476..5638)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6011..6027
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6047..6067
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggcagag gttgggaaag gagtcaacgc
      781 cgggaaactg gccagtaatg tacagaagag gttaacccga gcacaagaga aggtgatgca
      841 gaaactggga aaggcggatg aaaccaaaga cacagccttt gaggagcaag tagccaactt
      901 taacaaacag atgaatgagg gcaccaaact gcagaaagac ttgaaagcgt atttgctggc
      961 agtcaaaaca atgcacgagt gttccaagcg tctgcacgac tgtctggcag aaatgtatga
     1021 ccctgagtgg tttggcaaag aggaggtgga ctccatcgca gaggacactg acctcctgtg
     1081 gcaagatttc cacgagaagc ttttggacaa tgctgtaatt tccatggacg catacttggc
     1141 tcagtttcca gatataaagg ctcgcattgc aaagcgtgaa aggaagctgg tggactttga
     1201 cagtgccaga catcattttg cctctgtaca aaaaggcaag aaaaaggacg aggccaaaat
     1261 tgccaaggct gaggaacact tgggcagagc tcagaaagtg tttgaggaga tcaattatga
     1321 tctccaggag gaacttccca ccctgtggaa cagtcgtatt ggcctctatg ttaacacatt
     1381 ccggagtgtg tctggcctgg aggaaacatt tcacagggac atgggcaaac tgaaccagaa
     1441 tctgagtgat attatgacca aactggaaga gcagcgtgtt gccaaaaaag gtgtcacgac
     1501 agcaagcact gggaagagtg acgatgcagc aaaccacaat caggagacgt cagatgcacc
     1561 taaatctcca tcaaagaatg aagccccaac ggtgacaaat ggctcctcag atacagagct
     1621 tcctccatct gttctcttta aggtgaaaac aatgcacgac tacagtgcaa cagacagtga
     1681 tgaacttgac ctgaaggctg gtgacattgt gcttgtgctt ccctttgcca acccagatga
     1741 agcggatgat ggctggttaa tgggtgtaaa agagtcccac tggcttcaga acaaaaacct
     1801 cctagcaaaa ggagtcttcc ctgaaaactt cacccagaaa ctgtgacaac tttattatac
     1861 atagttgata attcactggc cgtcgtttta cggtaccatc gatgatgatc cagacatgat
     1921 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     1981 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2041 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2101 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat
     2161 ctgcgaagat acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc
     2221 gcagctgctg atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt
     2281 tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact
     2341 tccaaaggac caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag
     2401 gcagaataaa taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat
     2461 agcaagggaa aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa
     2521 aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa
     2581 gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac
     2641 agtaatcaag taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca
     2701 gcaagcccag cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt
     2761 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     2821 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2881 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2941 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3001 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3061 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3121 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3181 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3241 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3301 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3361 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3421 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3481 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3541 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3601 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3661 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3721 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3781 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3841 gaaaactcac gttaaggcat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3901 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3961 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4021 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4081 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4141 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4201 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4261 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4321 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4381 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4441 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4501 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4561 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4621 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4681 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4741 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4801 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4861 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4921 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     4981 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     5041 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     5101 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     5161 atcagggcga tggcccacgc tggctaagaa ctcatcagcc tccccggtcc atctacccac
     5221 gtaccaatgc accaattggc cacaatgacg gctactacat ggtgccattc cttcctcttt
     5281 ataggaatgg agactacctc ctgtccaaca atgctcttgg atacgagtac gcctacctgt
     5341 tggacccagg tcattgcaca acaccagaaa tgccctctga tctgcaaaag acgtgaatat
     5401 ctgttcagac acccatatcc actctgttcc acacaggtca gaggtttgtc caggagttct
     5461 tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa gtacttaggg
     5521 aaaattttac tcaattaaaa gtaaaagtat ctggctagaa tcttacttga gtaaaagtaa
     5581 aaaagtactc cattaaaatt gtacttgagt attaaggaag taaaagtaaa agcaagaaag
     5641 aaaactagag attcttgttt aagcttttaa tctcaaaaaa cattaaatga aatgcataca
     5701 aggttttatc ctgctttaga actgtttgta tttaattatc aaactataag acagacaatc
     5761 taatgccagt acacgctact caaagttgta aaacctcaga tttaacttca gtagaagctg
     5821 attctcaaaa ttgttagtgt caagcctagc tcttttgggg ctgaaaagca atcctgcagt
     5881 gctgaaaagc ctctcacagg cagccgatgc gggaagaggt gtattagtct tgatagagag
     5941 gctgcaaata gcaggaaacg tgagcagaga ctccctggtg tctgaaacac aggccagatg
     6001 ggccctcgag caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga
     6061 aaagttggca cgcgcagatt ccgtgtgggc ctggtaatat cttaccattt taagctattt
     6121 taagattagg gaaaaaaatt aaataaaact actttaaaat tactttcctt aaaaagcaac
     6181 atatttattt agtttttgtt taactggctc aagattgtaa tattttactt ttacatgttt
     6241 ttttaagtac ctaaatgtgt tcaaaagtaa agtatgttat tgtttttttt ttttaatgtt
     6301 agctaagctt taaattgtaa tatattactt ttaaaagtga ctttttaaaa atgtaaataa
     6361 aaaagtaact ataaatgtgt aatgcatgtt atttctgtgg gtttttaatt ttcatctcaa
     6421 ctgggcttac aattttaaca taataaaaat caaatcaaat caaatcaggc ccttatttgt
     6481 cacatacact cttgtatact gaaataaact aaatgttgag aaaaatttaa atacaccaaa
     6541 ataacgcata ccttttttgt aattacctaa gtaaaacatt tagttccttt tttaagtgac
     6601 tcaagattgt aatctattaa ttgtaaaaga taaaactagt gatcaatctg acgtgcttct
     6661 atgcagcctg tgagattaat tgtgatgttt aattattaaa caatttaaat ggatcaaaca
     6721 tttcacatgt gcttattgtg tccaacaaaa ccagcagaag cacttaaaaa tattgcatga
     6781 caacatgagt ttttaatgta aattatatat acttcctaaa cgaagttaat catatttcta
     6841 aaataataat gtcattgaaa tatttaaata aatgataagc tttagttgat tcaacataaa
     6901 attgaaagca ataaggactt ttttttttta cagcggtgaa gcaaaagcat gtaataatca
     6961 tataatataa taaataaata aataaataat ggctcttggt gcaaatatga acactttaaa
     7021 taaatataga ataaatatga tcaataagtc ataacgatgt gttttatgtt gattataatt
     7081 taaacatgtt tctaacgcaa gttttaaaaa ctgttttaaa gtattatttt aagttataag
     7141 atcaatttga ctcaacattt tgtagtgaaa atatgttgtg taaaattaat ataatataca
     7201 ctataaaaat gcagggttcc acacaataca ttaatgttgt cccaacacaa atcgattaag
     7261 ttaacaaata tatgtggatt gaacataaaa aattaagttg tcccaatgaa atctcaagaa
     7321 ttgtgttgtt tcagctcatt ttaaataagt agttttaaca agtaaaaaat aacatatttt
     7381 tgagtgtgta tatatatata ttacaaaata taataatata aaatgtaaaa gatataaacc
     7441 atgctacata tatttaagag ctattttgca cattactttt ttgcaagcca ttttagtgtt
     7501 ttttttacaa atatatatta gctaaatata caatatctta aatatacaat taatcagaca
     7561 tgcaattaat gcatttaacc tacataaaaa tacatgatca acatatgttt tatgctgatt
     7621 acaactaaat caaacgtaat gaaattaatt tataaaagcg atttaaatat gtcaatattt
     7681 taagttaaaa tcacttgaaa cctactataa atgaacttaa attaaaaaaa tctcctttaa
     7741 ttccaatgca acagtgttgt tttttcagct gaggaccccc ggtgcagatg gttggccccc
     7801 ggcccgctat tctaaggaag cctttcttaa ttttagggcg gcgctgcatg cgctcagatc
     7861 agattcacgg gtggagaggg ttattttctc ttttttattt tttcttgcct gctcatattt
     7921 gggcctgtgt gaaccccagg ctccgcctcg gcccccagga gccgctctta tttaaagggg
     7981 atcctcgcct tcagtcttcg tcccagtgga agcatcagtc ttgtgataac aacttggtga
     8041 gtcctacaga ccagcgctcg actctaggct ttaaatgcat tgatttctct ggattagcta
     8101 ccgggagagt tttaactttg cttattagtc ttgtgctcga actgctaggt ttaaagagtg
     8161 ttttctctaa tgcatgcatg aagcgacagc tgaaaaccag aaatagagcg tttgtcctcc
     8221 tgttaaactc tagcctttct ttggcatttg tcagtccgag tcttcagctg ccggcaggag
     8281 attgggtttc agtaacactt gctgagcaca aactacacgt gctttcttca gatttaattc
     8341 attattaatc attctagatt taaatgatgt tcttactcat tgctgattat tttagccttg
     8401 cggagcagat taaatgagca cgtgtttcat taagattgat ttaaatataa ttaatatttc
     8461 accactgtag ttttaaaatt gccttctaag tcatggttga tttttaatag gtatcggctc
     8521 taattagatt actattttgg tttttaaatg ttatttctat ttcatttgta ggctcgtttt
     8581 taatgtgatt ttattagatt ctagttttaa aatcatctct tagccttaat gagcagactg
     8641 aatgaacatg tttcattaag attaacttca ttataatgaa ttatttaatc attctagtgg
     8701 gttataaatc agctctattt aaattaattg tcgtgtttat ttctatttaa tttttgatta
     8761 ttttttcagg gtcgttttta tgtgattttt tcccgtttct aattttaaac taattgtttg
     8821 atttttatat gattcgactc tttttaaatg aattgttttg ctttttttat tgagcatata
     8881 tatattatac aatttcaatc aacaattgtt gtgcttttta aatgttgttt ctatttcatt
     8941 tctagggtcg attttattcg attctagttt taaaatcgtg atttttaact tctaacctta
     9001 ctgagcagac tgaatgaaca tgtttcatca agattaactt cattatagtt aattattgaa
     9061 tcgtgctagt ggtttataaa tcagctctat ttaaattaat catcatcatt atttctattt
     9121 aatttttatt atttctttag gattattttt atgtgatttt tttcacgttc tagttttaaa
     9181 ctcaatgctc attactgctc acccttgctg agcagtcttt cgttaagatt aaattaatta
     9241 ataaccattc tagttttaaa acagtgtctt aaggcatagc tgatatttta caagtttact
     9301 atttaatata tctcctttct ttttttaggg tcgatttcat ctgatttgat taatttttat
     9361 tatcttcact aagctttttg ttctaggttt tattctcagt attattactg ttatatttga
     9421 actgaatttc tcctcatagt ttattcattt tatttattta ttttttgttt attcgctatt
     9481 ttgtatttta gtttttaatt agaaatttta ttagaatcgt tttgaactag tttttatgca
     9541 ctctaacatt aatattaaat cataactatt atttttatta taatttattt gtgtttatct
     9601 ttttgtgtag ctttcatgtg tttttatttt agttttatta tgtttgcatt gaattttttg
     9661 gactagttta tataacgata tacagaaaag ccatgacaac aaatgttttt ataataccta
     9721 ttaagtataa gctaatcaga tttccactat tctgttcaaa gttaacatta ttagccattc
     9781 tgatggatca tttgacattt gtcatatgtc tttatgaaca ctgctctttc agatgccact
     9841 tttaaagctg ttttttacag taaatgaacg tttattatta tgacattatt tcctaaggcc
     9901 tctagagtac agcaggatgt ggcgtgtgca ttaccgtgta attatgctga ctgtaaatgt
     9961 gcatttatag actgtcagca tgtctgactg tgcgctgttg tgattggtcc tgcacagacc
    10021 aag
//
