LOCUS       sequence-220136-       10530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to EHD1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-EHD1a
FEATURES             Location/Qualifiers
     source          1..10530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2354..2374)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2382..2398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2414..2548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2438..2457)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2492..2511
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2987..3174,3175..3186)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3220..3236)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3220..3236)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3273..3293)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3273..3291)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3312..3328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3325..3347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3336..3352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3360..3366,3367..3384,3385..3390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3405..3426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3543..3560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3714..4302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3794..3813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4473..5264,5265..5333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5096..5115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5334..5438)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5601..5622)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5971..5982,5983..6145)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6518..6534
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6554..6574
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggctttcagc tggtcgaata gagatggtaa
      781 gaaagaccca gaacttttcc aaaacgtgtc cgatggactg aagcgccttt accgcaccaa
      841 actcttccct ctggaggaca cctatcattt ccacgacttc cattcaccgg cgctcgaaga
      901 tgcggatttt gacaacaagc ccatggtgct gctggtggga cagtactcga ccgggaagac
      961 caccttcatc aggcatttaa tggagcagga tttccccggg atgaggatag gcccagagcc
     1021 gacgacggat tccttcattg cggttatgca cggtgagcaa gaaggcgtca tacctggtaa
     1081 tgcgctggtg gtggatccga aaaagccttt ccgaaaactc aacgcatttg gcaacgcttt
     1141 cctcaacagg tttatgtgtg cacagttaaa caacccggta cttgagagca tcagtatcat
     1201 cgatactcca ggcatcctgt caggggagaa acagaggatc agcagaggct atgactttgc
     1261 tgctgttctg gagtggtttg cggagcgcgt ggaccgaatc atcctgctct tcgacgccca
     1321 caagctggac atttcggatg agttttcaga ggtgatcaag gccctgaaga accacgagga
     1381 caagatgcgc gtggtgctga ataaagcaga tcagatcagc acgcagcagc tcatgagggt
     1441 ttatggagcc ctcatgtggt cattgggcaa gatcataaac acccctgagg tggtgcgggt
     1501 ttatatcggc tccttctggg ctcagccgct gctcatcgct gaaaaccgga agctgtttga
     1561 agcggaggag caggatcttt tccaggatat tcagagtctg ccgagaaacg cagctctgag
     1621 gaaactcaac gatctgatca aaagagcacg tcttgctaag gtacatgctt acattatcag
     1681 tgctctgaag aaagagatgc cgagcgtgtt tggcaaagac tcaaagaaga aagagcttat
     1741 taacaacttg ggcgctatat atgaaaagat ccagaaagag cacaacattt caccaggaga
     1801 ctttccaaac cttaagaaaa tgcaggagtt gctatctgtt caggacttca ccaaattccc
     1861 tgccatgaag cccaaacttc tagagtcggt ggaagacatg ctaggtaacg acatcgctaa
     1921 gctaatggca ctggtgcgta aagaggaggc caccatgccc agccaatcag tgaagggcgg
     1981 tgcttttgag ggaatctcaa acggcccgtt cggtcatggg tacggagaag gcgccggcga
     2041 aggcatcgac gagctggagt ggatcgtggg ccgtgacaaa ccatcctacg atgaaatttt
     2101 ctacaccctc tctccgatca acgggaagat ctccggagcc acagctaaga aggagatgac
     2161 gaagtccaag ctgccgaaca cagtgctggg gaaaatctgg actctggcag atgtggataa
     2221 agatggatac ctggatgacg aagagtttgc tctggccaat cacctgatca aggtaaagct
     2281 tgagggcttc gagctcccgg ctaaacttcc caatcatctt gtgcctccgt ccaaacggca
     2341 agagcacaat tgacaacttt attatacata gttgataatt cactggccgt cgttttacgg
     2401 taccatcgat gatgatccag acatgataag atacattgat gagtttggac aaaccacaac
     2461 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2521 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2581 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     2641 ggctgattat gatcctctag atcagatctg cgaagatacg gccacgggtg ctcttgatcc
     2701 tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt
     2761 ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg
     2821 tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt
     2881 catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca
     2941 tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa
     3001 aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta
     3061 aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa
     3121 aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca
     3181 cctctggttc ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg
     3241 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     3301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     3481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     3541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4141 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aaggcatttt ggtcatgaga
     4381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5461 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     5521 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     5581 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     5641 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccacgctgg ctaagaactc
     5701 atcagcctcc ccggtccatc tacccacgta ccaatgcacc aattggccac aatgacggct
     5761 actacatggt gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaatg
     5821 ctcttggata cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc
     5881 cctctgatct gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca
     5941 caggtcagag gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta
     6001 ctcaagtgaa agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg
     6061 gctagaatct tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt
     6121 aaggaagtaa aagtaaaagc aagaaagaaa actagagatt cttgtttaag cttttaatct
     6181 caaaaaacat taaatgaaat gcatacaagg ttttatcctg ctttagaact gtttgtattt
     6241 aattatcaaa ctataagaca gacaatctaa tgccagtaca cgctactcaa agttgtaaaa
     6301 cctcagattt aacttcagta gaagctgatt ctcaaaattg ttagtgtcaa gcctagctct
     6361 tttggggctg aaaagcaatc ctgcagtgct gaaaagcctc tcacaggcag ccgatgcggg
     6421 aagaggtgta ttagtcttga tagagaggct gcaaatagca ggaaacgtga gcagagactc
     6481 cctggtgtct gaaacacagg ccagatgggc cctcgagcag gaaacagcta tgaccatgat
     6541 tacgccaagc tatcaacttt gtatagaaaa gttggcacgc gcagattccg tgtgggcctg
     6601 gtaatatctt accattttaa gctattttaa gattagggaa aaaaattaaa taaaactact
     6661 ttaaaattac tttccttaaa aagcaacata tttatttagt ttttgtttaa ctggctcaag
     6721 attgtaatat tttactttta catgtttttt taagtaccta aatgtgttca aaagtaaagt
     6781 atgttattgt tttttttttt taatgttagc taagctttaa attgtaatat attactttta
     6841 aaagtgactt tttaaaaatg taaataaaaa agtaactata aatgtgtaat gcatgttatt
     6901 tctgtgggtt tttaattttc atctcaactg ggcttacaat tttaacataa taaaaatcaa
     6961 atcaaatcaa atcaggccct tatttgtcac atacactctt gtatactgaa ataaactaaa
     7021 tgttgagaaa aatttaaata caccaaaata acgcatacct tttttgtaat tacctaagta
     7081 aaacatttag ttcctttttt aagtgactca agattgtaat ctattaattg taaaagataa
     7141 aactagtgat caatctgacg tgcttctatg cagcctgtga gattaattgt gatgtttaat
     7201 tattaaacaa tttaaatgga tcaaacattt cacatgtgct tattgtgtcc aacaaaacca
     7261 gcagaagcac ttaaaaatat tgcatgacaa catgagtttt taatgtaaat tatatatact
     7321 tcctaaacga agttaatcat atttctaaaa taataatgtc attgaaatat ttaaataaat
     7381 gataagcttt agttgattca acataaaatt gaaagcaata aggacttttt ttttttacag
     7441 cggtgaagca aaagcatgta ataatcatat aatataataa ataaataaat aaataatggc
     7501 tcttggtgca aatatgaaca ctttaaataa atatagaata aatatgatca ataagtcata
     7561 acgatgtgtt ttatgttgat tataatttaa acatgtttct aacgcaagtt ttaaaaactg
     7621 ttttaaagta ttattttaag ttataagatc aatttgactc aacattttgt agtgaaaata
     7681 tgttgtgtaa aattaatata atatacacta taaaaatgca gggttccaca caatacatta
     7741 atgttgtccc aacacaaatc gattaagtta acaaatatat gtggattgaa cataaaaaat
     7801 taagttgtcc caatgaaatc tcaagaattg tgttgtttca gctcatttta aataagtagt
     7861 tttaacaagt aaaaaataac atatttttga gtgtgtatat atatatatta caaaatataa
     7921 taatataaaa tgtaaaagat ataaaccatg ctacatatat ttaagagcta ttttgcacat
     7981 tacttttttg caagccattt tagtgttttt tttacaaata tatattagct aaatatacaa
     8041 tatcttaaat atacaattaa tcagacatgc aattaatgca tttaacctac ataaaaatac
     8101 atgatcaaca tatgttttat gctgattaca actaaatcaa acgtaatgaa attaatttat
     8161 aaaagcgatt taaatatgtc aatattttaa gttaaaatca cttgaaacct actataaatg
     8221 aacttaaatt aaaaaaatct cctttaattc caatgcaaca gtgttgtttt ttcagctgag
     8281 gacccccggt gcagatggtt ggcccccggc ccgctattct aaggaagcct ttcttaattt
     8341 tagggcggcg ctgcatgcgc tcagatcaga ttcacgggtg gagagggtta ttttctcttt
     8401 tttatttttt cttgcctgct catatttggg cctgtgtgaa ccccaggctc cgcctcggcc
     8461 cccaggagcc gctcttattt aaaggggatc ctcgccttca gtcttcgtcc cagtggaagc
     8521 atcagtcttg tgataacaac ttggtgagtc ctacagacca gcgctcgact ctaggcttta
     8581 aatgcattga tttctctgga ttagctaccg ggagagtttt aactttgctt attagtcttg
     8641 tgctcgaact gctaggttta aagagtgttt tctctaatgc atgcatgaag cgacagctga
     8701 aaaccagaaa tagagcgttt gtcctcctgt taaactctag cctttctttg gcatttgtca
     8761 gtccgagtct tcagctgccg gcaggagatt gggtttcagt aacacttgct gagcacaaac
     8821 tacacgtgct ttcttcagat ttaattcatt attaatcatt ctagatttaa atgatgttct
     8881 tactcattgc tgattatttt agccttgcgg agcagattaa atgagcacgt gtttcattaa
     8941 gattgattta aatataatta atatttcacc actgtagttt taaaattgcc ttctaagtca
     9001 tggttgattt ttaataggta tcggctctaa ttagattact attttggttt ttaaatgtta
     9061 tttctatttc atttgtaggc tcgtttttaa tgtgatttta ttagattcta gttttaaaat
     9121 catctcttag ccttaatgag cagactgaat gaacatgttt cattaagatt aacttcatta
     9181 taatgaatta tttaatcatt ctagtgggtt ataaatcagc tctatttaaa ttaattgtcg
     9241 tgtttatttc tatttaattt ttgattattt tttcagggtc gtttttatgt gattttttcc
     9301 cgtttctaat tttaaactaa ttgtttgatt tttatatgat tcgactcttt ttaaatgaat
     9361 tgttttgctt tttttattga gcatatatat attatacaat ttcaatcaac aattgttgtg
     9421 ctttttaaat gttgtttcta tttcatttct agggtcgatt ttattcgatt ctagttttaa
     9481 aatcgtgatt tttaacttct aaccttactg agcagactga atgaacatgt ttcatcaaga
     9541 ttaacttcat tatagttaat tattgaatcg tgctagtggt ttataaatca gctctattta
     9601 aattaatcat catcattatt tctatttaat ttttattatt tctttaggat tatttttatg
     9661 tgattttttt cacgttctag ttttaaactc aatgctcatt actgctcacc cttgctgagc
     9721 agtctttcgt taagattaaa ttaattaata accattctag ttttaaaaca gtgtcttaag
     9781 gcatagctga tattttacaa gtttactatt taatatatct cctttctttt tttagggtcg
     9841 atttcatctg atttgattaa tttttattat cttcactaag ctttttgttc taggttttat
     9901 tctcagtatt attactgtta tatttgaact gaatttctcc tcatagttta ttcattttat
     9961 ttatttattt tttgtttatt cgctattttg tattttagtt tttaattaga aattttatta
    10021 gaatcgtttt gaactagttt ttatgcactc taacattaat attaaatcat aactattatt
    10081 tttattataa tttatttgtg tttatctttt tgtgtagctt tcatgtgttt ttattttagt
    10141 tttattatgt ttgcattgaa ttttttggac tagtttatat aacgatatac agaaaagcca
    10201 tgacaacaaa tgtttttata atacctatta agtataagct aatcagattt ccactattct
    10261 gttcaaagtt aacattatta gccattctga tggatcattt gacatttgtc atatgtcttt
    10321 atgaacactg ctctttcaga tgccactttt aaagctgttt tttacagtaa atgaacgttt
    10381 attattatga cattatttcc taaggcctct agagtacagc aggatgtggc gtgtgcatta
    10441 ccgtgtaatt atgctgactg taaatgtgca tttatagact gtcagcatgt ctgactgtgc
    10501 gctgttgtga ttggtcctgc acagaccaag
//
