LOCUS       sequence-220139-       10233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to 
            EHD1b-delta-EH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-EHD1b-dEH
FEATURES             Location/Qualifiers
     source          1..10233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2057..2077)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2085..2101)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2117..2251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2141..2160)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2195..2214
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2690..2877,2878..2889)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2923..2939)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2923..2939)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2976..2996)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2976..2994)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3015..3031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3028..3050)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3039..3055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3063..3069,3070..3087,3088..3093))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3108..3129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3246..3263)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3417..4005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3497..3516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4176..4967,4968..5036))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4799..4818
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5037..5141)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5304..5325)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5674..5685,5686..5848)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6221..6237
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6257..6277
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggctttcagt tggtctaaca aagatggaaa
      781 aaagaaagac ccggagttat ttcaaacggt ttcggacggg ctaaagagac tgtacaggac
      841 caaacttttc cccttagagg acgcataccg ctttcacgac ttccactctc cggctctgga
      901 agatgctgat ttcgataaca agcccatggt acttttggtg ggccaatact ctacagggaa
      961 aaccactttt attcggcatc tcatggagca ggatttccct ggtatgcgca tagggcccga
     1021 accgaccacc gattcattca tagcggttat gcacggggaa caagaaggtc tcatccccgg
     1081 caatgcttta gtggttgacc ccaagaaacc cttcagaaaa ctcaacgcct ttggaaacgc
     1141 ctttctgaac aggttcatat gtgctcagat gccaaaccca gtgctggaca gcatcagcat
     1201 catagacact cctggaattc tgtccggaga gaaacaacgg atcagcagag gttatgattt
     1261 tgctgcggtg cttgaatggt ttgccgaacg ggtggaccga atcattcttc tctttgacgc
     1321 acacaagttg gacatctcgg atgagttctc cgaggtgatc cgagctctga aaaaccatga
     1381 ggacaaaatg cgggtggtgc ttaataaagc tgatcagatc gggacccagc agcttatgag
     1441 ggtgtatggc gccctcatgt ggtcacttgg gaaaattgtc aacactccag aggtcatacg
     1501 tgtgtatatt ggctctttct gggctcagcc gctcctggtt gcagataaca ggaaattgtt
     1561 tgaggcagag gaacaagacc ttttccgtga catccaggga cttccacgta atgcagcact
     1621 gcgcaaactg aatgacctta tcaaacgggc ccgtctagcc aaagttcaag catacatcat
     1681 cagctctttg aagaaagaaa tgccaaatgt gtttggaaaa gagaacaaga aaaaggagct
     1741 cattgctaac ctgggtgaaa tctatgcaaa gattgaaaaa gaacatcaga tctcccctgg
     1801 tgattttcct aaactcagta aaatgcagga agtcctcgct ggacaggact tcactaagtt
     1861 tcaaggattg aagcccaagc ttttggaatc agtggaagac atgctggcca acgacattgc
     1921 caaactcatg accctcgtcc ggcaggagga ggcctccatg ccaagccagg ctgtccaagg
     1981 tggagccttt gaaggcacca tgaatggccc cttcgggcac ggctacggag aaggtgccgg
     2041 tgaaggcatt gattaacaac tttattatac atagttgata attcactggc cgtcgtttta
     2101 cggtaccatc gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     2161 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     2221 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     2281 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     2341 tatggctgat tatgatcctc tagatcagat ctgcgaagat acggccacgg gtgctcttga
     2401 tcctgtggct gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc
     2461 attttcttcc actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca
     2521 ttgtggtaat agatgtgaca tgacgtcact tccaaaggac caatgaacat gtctgaccaa
     2581 tttcatataa tgtgaaaacg attttcatag gcagaataaa taacatttaa attaaactgg
     2641 gcatcagcgc aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc
     2701 aaaaaataag tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt
     2761 ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag
     2821 taaaaatccc caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt
     2881 acacctctgg ttcttgaccc cctaccttca gcaagcccag cagatccact agttctagag
     2941 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
     3001 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3061 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3841 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaaggcat tttggtcatg
     4081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5161 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     5221 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     5281 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     5341 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
     5401 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
     5461 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
     5521 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
     5581 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
     5641 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
     5701 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
     5761 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
     5821 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
     5881 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
     5941 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
     6001 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
     6061 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
     6121 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     6181 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     6241 gattacgcca agctatcaac tttgtataga aaagttggca cgcgcagatt ccgtgtgggc
     6301 ctggtaatat cttaccattt taagctattt taagattagg gaaaaaaatt aaataaaact
     6361 actttaaaat tactttcctt aaaaagcaac atatttattt agtttttgtt taactggctc
     6421 aagattgtaa tattttactt ttacatgttt ttttaagtac ctaaatgtgt tcaaaagtaa
     6481 agtatgttat tgtttttttt ttttaatgtt agctaagctt taaattgtaa tatattactt
     6541 ttaaaagtga ctttttaaaa atgtaaataa aaaagtaact ataaatgtgt aatgcatgtt
     6601 atttctgtgg gtttttaatt ttcatctcaa ctgggcttac aattttaaca taataaaaat
     6661 caaatcaaat caaatcaggc ccttatttgt cacatacact cttgtatact gaaataaact
     6721 aaatgttgag aaaaatttaa atacaccaaa ataacgcata ccttttttgt aattacctaa
     6781 gtaaaacatt tagttccttt tttaagtgac tcaagattgt aatctattaa ttgtaaaaga
     6841 taaaactagt gatcaatctg acgtgcttct atgcagcctg tgagattaat tgtgatgttt
     6901 aattattaaa caatttaaat ggatcaaaca tttcacatgt gcttattgtg tccaacaaaa
     6961 ccagcagaag cacttaaaaa tattgcatga caacatgagt ttttaatgta aattatatat
     7021 acttcctaaa cgaagttaat catatttcta aaataataat gtcattgaaa tatttaaata
     7081 aatgataagc tttagttgat tcaacataaa attgaaagca ataaggactt ttttttttta
     7141 cagcggtgaa gcaaaagcat gtaataatca tataatataa taaataaata aataaataat
     7201 ggctcttggt gcaaatatga acactttaaa taaatataga ataaatatga tcaataagtc
     7261 ataacgatgt gttttatgtt gattataatt taaacatgtt tctaacgcaa gttttaaaaa
     7321 ctgttttaaa gtattatttt aagttataag atcaatttga ctcaacattt tgtagtgaaa
     7381 atatgttgtg taaaattaat ataatataca ctataaaaat gcagggttcc acacaataca
     7441 ttaatgttgt cccaacacaa atcgattaag ttaacaaata tatgtggatt gaacataaaa
     7501 aattaagttg tcccaatgaa atctcaagaa ttgtgttgtt tcagctcatt ttaaataagt
     7561 agttttaaca agtaaaaaat aacatatttt tgagtgtgta tatatatata ttacaaaata
     7621 taataatata aaatgtaaaa gatataaacc atgctacata tatttaagag ctattttgca
     7681 cattactttt ttgcaagcca ttttagtgtt ttttttacaa atatatatta gctaaatata
     7741 caatatctta aatatacaat taatcagaca tgcaattaat gcatttaacc tacataaaaa
     7801 tacatgatca acatatgttt tatgctgatt acaactaaat caaacgtaat gaaattaatt
     7861 tataaaagcg atttaaatat gtcaatattt taagttaaaa tcacttgaaa cctactataa
     7921 atgaacttaa attaaaaaaa tctcctttaa ttccaatgca acagtgttgt tttttcagct
     7981 gaggaccccc ggtgcagatg gttggccccc ggcccgctat tctaaggaag cctttcttaa
     8041 ttttagggcg gcgctgcatg cgctcagatc agattcacgg gtggagaggg ttattttctc
     8101 ttttttattt tttcttgcct gctcatattt gggcctgtgt gaaccccagg ctccgcctcg
     8161 gcccccagga gccgctctta tttaaagggg atcctcgcct tcagtcttcg tcccagtgga
     8221 agcatcagtc ttgtgataac aacttggtga gtcctacaga ccagcgctcg actctaggct
     8281 ttaaatgcat tgatttctct ggattagcta ccgggagagt tttaactttg cttattagtc
     8341 ttgtgctcga actgctaggt ttaaagagtg ttttctctaa tgcatgcatg aagcgacagc
     8401 tgaaaaccag aaatagagcg tttgtcctcc tgttaaactc tagcctttct ttggcatttg
     8461 tcagtccgag tcttcagctg ccggcaggag attgggtttc agtaacactt gctgagcaca
     8521 aactacacgt gctttcttca gatttaattc attattaatc attctagatt taaatgatgt
     8581 tcttactcat tgctgattat tttagccttg cggagcagat taaatgagca cgtgtttcat
     8641 taagattgat ttaaatataa ttaatatttc accactgtag ttttaaaatt gccttctaag
     8701 tcatggttga tttttaatag gtatcggctc taattagatt actattttgg tttttaaatg
     8761 ttatttctat ttcatttgta ggctcgtttt taatgtgatt ttattagatt ctagttttaa
     8821 aatcatctct tagccttaat gagcagactg aatgaacatg tttcattaag attaacttca
     8881 ttataatgaa ttatttaatc attctagtgg gttataaatc agctctattt aaattaattg
     8941 tcgtgtttat ttctatttaa tttttgatta ttttttcagg gtcgttttta tgtgattttt
     9001 tcccgtttct aattttaaac taattgtttg atttttatat gattcgactc tttttaaatg
     9061 aattgttttg ctttttttat tgagcatata tatattatac aatttcaatc aacaattgtt
     9121 gtgcttttta aatgttgttt ctatttcatt tctagggtcg attttattcg attctagttt
     9181 taaaatcgtg atttttaact tctaacctta ctgagcagac tgaatgaaca tgtttcatca
     9241 agattaactt cattatagtt aattattgaa tcgtgctagt ggtttataaa tcagctctat
     9301 ttaaattaat catcatcatt atttctattt aatttttatt atttctttag gattattttt
     9361 atgtgatttt tttcacgttc tagttttaaa ctcaatgctc attactgctc acccttgctg
     9421 agcagtcttt cgttaagatt aaattaatta ataaccattc tagttttaaa acagtgtctt
     9481 aaggcatagc tgatatttta caagtttact atttaatata tctcctttct ttttttaggg
     9541 tcgatttcat ctgatttgat taatttttat tatcttcact aagctttttg ttctaggttt
     9601 tattctcagt attattactg ttatatttga actgaatttc tcctcatagt ttattcattt
     9661 tatttattta ttttttgttt attcgctatt ttgtatttta gtttttaatt agaaatttta
     9721 ttagaatcgt tttgaactag tttttatgca ctctaacatt aatattaaat cataactatt
     9781 atttttatta taatttattt gtgtttatct ttttgtgtag ctttcatgtg tttttatttt
     9841 agttttatta tgtttgcatt gaattttttg gactagttta tataacgata tacagaaaag
     9901 ccatgacaac aaatgttttt ataataccta ttaagtataa gctaatcaga tttccactat
     9961 tctgttcaaa gttaacatta ttagccattc tgatggatca tttgacattt gtcatatgtc
    10021 tttatgaaca ctgctctttc agatgccact tttaaagctg ttttttacag taaatgaacg
    10081 tttattatta tgacattatt tcctaaggcc tctagagtac agcaggatgt ggcgtgtgca
    10141 ttaccgtgta attatgctga ctgtaaatgt gcatttatag actgtcagca tgtctgactg
    10201 tgcgctgttg tgattggtcc tgcacagacc aag
//
