LOCUS       sequence-220140-       10569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to EHD2a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-EHD2a
FEATURES             Location/Qualifiers
     source          1..10569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2393..2413)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2421..2437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2453..2587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2477..2496)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2531..2550
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(3026..3213,3214..3225)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3259..3275)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3259..3275)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3312..3332)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3312..3330)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3351..3367)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3364..3386)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3375..3391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3399..3405,3406..3423,3424..3429))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3444..3465
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3582..3599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3753..4341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3833..3852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4512..5303,5304..5372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5135..5154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5373..5477)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5640..5661)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6010..6021,6022..6184)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6557..6573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6593..6613
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgtctgcc aaaggaatga ggaacagggg
      781 tggtaaatgt ctgaaggcgg ttcatacagt aaccgaggct ttgaaaatcc tctatcggca
      841 aaaactgctc ccactggaga agtactatgg tttctccgat ttccactcat caagccttga
      901 agatgcagac tttgacaaca aacccatgat actggtgttg ggccagtgct ctacaggcaa
      961 gaccactttt atcaggtatc ttcttgaaca ggagtatcct ggaagcagag ttggcccaga
     1021 accgacaacc gacagtttca cagcaataat gcatggagag atggagggaa ttattcatgg
     1081 caatgcttta actgttgatc ccaataagcc tttccgcaaa ctcagcccat ttggaacaac
     1141 tttcctaaat cgattccagt gtgtacatct gcccaatcgg gtcctagaga gcatcagcat
     1201 catcgacaca ccagggatcc tgtctgtggc caagcgcaaa atggacagag gttatgactt
     1261 tccttcagtt atccgctggt ttgcggagca cgtagaccgg atcatcctcc tgtgtgatgc
     1321 acacaagctg gagatttctg atgagttttc ccgcactata ctggcattac gaggcaatga
     1381 ggacaagctc cgagtggtgc tcaacaaggc cgacatggtg gactcgcagg agctgatgcg
     1441 ggtctacggc gccctcatgt ggtctctcgg gaaggtcttc tgcaccccgg aggtcctgag
     1501 ggtctacatc ggctccttct ggaccgctcc gatgcgaatc acagacaacc gcaaactgtt
     1561 tgagcgagag gagggagatc tttttgcaga ccttcagaac ctgccaagaa attctgccct
     1621 gagaaaactc aatgatctgg tgaaacgagc acgccttgta agggctcatg cttatatcat
     1681 cagctgtcta aaagacgaga tgcctgttgt tttctgcaga ggaaagaagc ggcgagatct
     1741 catctatgac cttcccacca tctattccag aatccaacag cgctatcaga tttcaccagg
     1801 agattttcca gactgtgcca agatgcagga gcgactcatg agccacgact tcaagtttaa
     1861 aggcctcaag cccaagctct tagctgggtt ggatgagctc ctaaacaccg acattgctaa
     1921 gctgatgcct cttctacgcc aagaggaaga agaagcagct gatcaacttt taatacaagg
     1981 aggaccttta gggtctcaca ggggcccgtt tattgtgggg aacccatttc atcactctgg
     2041 atccaatgga gaaatcgatc aggcgtcaaa tgatgaatgg gtggtgtcaa aagataaagc
     2101 aaagtacgac gagatctttt acaacctctc gcctcatgag ggaaagctga ctgggactaa
     2161 agtgaaggag tggatgttgc gcactcatct gcccagctcg gtcctgggac gaatctggaa
     2221 gctggcagat gtggattgtg atggtaagtt ggacgatgag gagtttgctt tggccggtca
     2281 tctcattgag gtaaagttgg aaggttttgg gcttcctcat gagctgccca cacacctgct
     2341 gccaccgtct aaaagacacc gcaagaactc tgagattacc gacgatgatt agcaacttta
     2401 ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga
     2461 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2521 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2581 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2641 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
     2701 tcagatctgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg
     2761 ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt
     2821 ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac
     2881 gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt
     2941 tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt
     3001 ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt
     3061 aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa
     3121 gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt
     3181 aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta
     3241 ccttcagcaa gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc
     3301 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     3361 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3421 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3481 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3541 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3601 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3661 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4021 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4081 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4141 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4201 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4261 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4321 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4381 tggaacgaaa actcacgtta aggcattttg gtcatgagat tatcaaaaag gatcttcacc
     4441 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4501 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4561 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4621 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4681 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4741 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4801 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4861 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4921 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4981 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5041 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5101 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5161 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5221 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5281 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5341 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5401 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5461 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     5521 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     5581 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     5641 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     5701 ccgtctatca gggcgatggc ccacgctggc taagaactca tcagcctccc cggtccatct
     5761 acccacgtac caatgcacca attggccaca atgacggcta ctacatggtg ccattccttc
     5821 ctctttatag gaatggagac tacctcctgt ccaacaatgc tcttggatac gagtacgcct
     5881 acctgttgga cccaggtcat tgcacaacac cagaaatgcc ctctgatctg caaaagacgt
     5941 gaatatctgt tcagacaccc atatccactc tgttccacac aggtcagagg tttgtccagg
     6001 agttcttgac agaggtgtaa aaagtactca aaaattttac tcaagtgaaa gtacaagtac
     6061 ttagggaaaa ttttactcaa ttaaaagtaa aagtatctgg ctagaatctt acttgagtaa
     6121 aagtaaaaaa gtactccatt aaaattgtac ttgagtatta aggaagtaaa agtaaaagca
     6181 agaaagaaaa ctagagattc ttgtttaagc ttttaatctc aaaaaacatt aaatgaaatg
     6241 catacaaggt tttatcctgc tttagaactg tttgtattta attatcaaac tataagacag
     6301 acaatctaat gccagtacac gctactcaaa gttgtaaaac ctcagattta acttcagtag
     6361 aagctgattc tcaaaattgt tagtgtcaag cctagctctt ttggggctga aaagcaatcc
     6421 tgcagtgctg aaaagcctct cacaggcagc cgatgcggga agaggtgtat tagtcttgat
     6481 agagaggctg caaatagcag gaaacgtgag cagagactcc ctggtgtctg aaacacaggc
     6541 cagatgggcc ctcgagcagg aaacagctat gaccatgatt acgccaagct atcaactttg
     6601 tatagaaaag ttggcacgcg cagattccgt gtgggcctgg taatatctta ccattttaag
     6661 ctattttaag attagggaaa aaaattaaat aaaactactt taaaattact ttccttaaaa
     6721 agcaacatat ttatttagtt tttgtttaac tggctcaaga ttgtaatatt ttacttttac
     6781 atgttttttt aagtacctaa atgtgttcaa aagtaaagta tgttattgtt tttttttttt
     6841 aatgttagct aagctttaaa ttgtaatata ttacttttaa aagtgacttt ttaaaaatgt
     6901 aaataaaaaa gtaactataa atgtgtaatg catgttattt ctgtgggttt ttaattttca
     6961 tctcaactgg gcttacaatt ttaacataat aaaaatcaaa tcaaatcaaa tcaggccctt
     7021 atttgtcaca tacactcttg tatactgaaa taaactaaat gttgagaaaa atttaaatac
     7081 accaaaataa cgcatacctt ttttgtaatt acctaagtaa aacatttagt tcctttttta
     7141 agtgactcaa gattgtaatc tattaattgt aaaagataaa actagtgatc aatctgacgt
     7201 gcttctatgc agcctgtgag attaattgtg atgtttaatt attaaacaat ttaaatggat
     7261 caaacatttc acatgtgctt attgtgtcca acaaaaccag cagaagcact taaaaatatt
     7321 gcatgacaac atgagttttt aatgtaaatt atatatactt cctaaacgaa gttaatcata
     7381 tttctaaaat aataatgtca ttgaaatatt taaataaatg ataagcttta gttgattcaa
     7441 cataaaattg aaagcaataa ggactttttt tttttacagc ggtgaagcaa aagcatgtaa
     7501 taatcatata atataataaa taaataaata aataatggct cttggtgcaa atatgaacac
     7561 tttaaataaa tatagaataa atatgatcaa taagtcataa cgatgtgttt tatgttgatt
     7621 ataatttaaa catgtttcta acgcaagttt taaaaactgt tttaaagtat tattttaagt
     7681 tataagatca atttgactca acattttgta gtgaaaatat gttgtgtaaa attaatataa
     7741 tatacactat aaaaatgcag ggttccacac aatacattaa tgttgtccca acacaaatcg
     7801 attaagttaa caaatatatg tggattgaac ataaaaaatt aagttgtccc aatgaaatct
     7861 caagaattgt gttgtttcag ctcattttaa ataagtagtt ttaacaagta aaaaataaca
     7921 tatttttgag tgtgtatata tatatattac aaaatataat aatataaaat gtaaaagata
     7981 taaaccatgc tacatatatt taagagctat tttgcacatt acttttttgc aagccatttt
     8041 agtgtttttt ttacaaatat atattagcta aatatacaat atcttaaata tacaattaat
     8101 cagacatgca attaatgcat ttaacctaca taaaaataca tgatcaacat atgttttatg
     8161 ctgattacaa ctaaatcaaa cgtaatgaaa ttaatttata aaagcgattt aaatatgtca
     8221 atattttaag ttaaaatcac ttgaaaccta ctataaatga acttaaatta aaaaaatctc
     8281 ctttaattcc aatgcaacag tgttgttttt tcagctgagg acccccggtg cagatggttg
     8341 gcccccggcc cgctattcta aggaagcctt tcttaatttt agggcggcgc tgcatgcgct
     8401 cagatcagat tcacgggtgg agagggttat tttctctttt ttattttttc ttgcctgctc
     8461 atatttgggc ctgtgtgaac cccaggctcc gcctcggccc ccaggagccg ctcttattta
     8521 aaggggatcc tcgccttcag tcttcgtccc agtggaagca tcagtcttgt gataacaact
     8581 tggtgagtcc tacagaccag cgctcgactc taggctttaa atgcattgat ttctctggat
     8641 tagctaccgg gagagtttta actttgctta ttagtcttgt gctcgaactg ctaggtttaa
     8701 agagtgtttt ctctaatgca tgcatgaagc gacagctgaa aaccagaaat agagcgtttg
     8761 tcctcctgtt aaactctagc ctttctttgg catttgtcag tccgagtctt cagctgccgg
     8821 caggagattg ggtttcagta acacttgctg agcacaaact acacgtgctt tcttcagatt
     8881 taattcatta ttaatcattc tagatttaaa tgatgttctt actcattgct gattatttta
     8941 gccttgcgga gcagattaaa tgagcacgtg tttcattaag attgatttaa atataattaa
     9001 tatttcacca ctgtagtttt aaaattgcct tctaagtcat ggttgatttt taataggtat
     9061 cggctctaat tagattacta ttttggtttt taaatgttat ttctatttca tttgtaggct
     9121 cgtttttaat gtgattttat tagattctag ttttaaaatc atctcttagc cttaatgagc
     9181 agactgaatg aacatgtttc attaagatta acttcattat aatgaattat ttaatcattc
     9241 tagtgggtta taaatcagct ctatttaaat taattgtcgt gtttatttct atttaatttt
     9301 tgattatttt ttcagggtcg tttttatgtg attttttccc gtttctaatt ttaaactaat
     9361 tgtttgattt ttatatgatt cgactctttt taaatgaatt gttttgcttt ttttattgag
     9421 catatatata ttatacaatt tcaatcaaca attgttgtgc tttttaaatg ttgtttctat
     9481 ttcatttcta gggtcgattt tattcgattc tagttttaaa atcgtgattt ttaacttcta
     9541 accttactga gcagactgaa tgaacatgtt tcatcaagat taacttcatt atagttaatt
     9601 attgaatcgt gctagtggtt tataaatcag ctctatttaa attaatcatc atcattattt
     9661 ctatttaatt tttattattt ctttaggatt atttttatgt gatttttttc acgttctagt
     9721 tttaaactca atgctcatta ctgctcaccc ttgctgagca gtctttcgtt aagattaaat
     9781 taattaataa ccattctagt tttaaaacag tgtcttaagg catagctgat attttacaag
     9841 tttactattt aatatatctc ctttcttttt ttagggtcga tttcatctga tttgattaat
     9901 ttttattatc ttcactaagc tttttgttct aggttttatt ctcagtatta ttactgttat
     9961 atttgaactg aatttctcct catagtttat tcattttatt tatttatttt ttgtttattc
    10021 gctattttgt attttagttt ttaattagaa attttattag aatcgttttg aactagtttt
    10081 tatgcactct aacattaata ttaaatcata actattattt ttattataat ttatttgtgt
    10141 ttatcttttt gtgtagcttt catgtgtttt tattttagtt ttattatgtt tgcattgaat
    10201 tttttggact agtttatata acgatataca gaaaagccat gacaacaaat gtttttataa
    10261 tacctattaa gtataagcta atcagatttc cactattctg ttcaaagtta acattattag
    10321 ccattctgat ggatcatttg acatttgtca tatgtcttta tgaacactgc tctttcagat
    10381 gccactttta aagctgtttt ttacagtaaa tgaacgttta ttattatgac attatttcct
    10441 aaggcctcta gagtacagca ggatgtggcg tgtgcattac cgtgtaatta tgctgactgt
    10501 aaatgtgcat ttatagactg tcagcatgtc tgactgtgcg ctgttgtgat tggtcctgca
    10561 cagaccaag
//
