LOCUS       sequence-220156-       11091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Hara T, Takamura A, Kishi C, Iemura S, Natsume T, Guan JL, Mizushima
            N
  TITLE     FIP200, a ULK-interacting protein, is required for autophagosome 
            formation in mammalian cells.
  JOURNAL   J Cell Biol. 2008 May 5. 181(3):497-510.
  PUBMED    18443221
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2008 May 5. 181(3):497-510."
COMMENT     Sequence Label: p3xFLAG-CMV10-hFIP200
FEATURES             Location/Qualifiers
     source          1..11091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     582..604
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     596..613
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     597..613
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        774..1153
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1154..1357
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1308..1328
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1387..1452
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4152..4163
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4395..4406
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6306..6928
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6505..6524)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6952..6972)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6970..7267
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7321..8115
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7375..7394)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7985..8004
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8767..8901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8804..8823)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8858..8877
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8906..8925)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8907..8925)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8939..8955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8939..8955)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8952..8974)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8963..8979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8987..8993,8994..9011,9012..9017))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9032..9053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9170..9187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9341..9929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9421..9440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10100..10891,10892..10960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10723..10742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10961..11065)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
      481 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      541 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      601 aacgacggcc agtgccaagc tgatctatac attgaatcaa tattggcaat tagccatatt
      661 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
      721 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
      781 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      841 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      901 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      961 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     1021 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     1081 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     1141 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     1201 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1261 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     1321 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagaatta
     1381 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     1441 gacgatgaca agcttgcggc cgcgaattct atgaagttat atgtatttct ggttaacact
     1501 ggaactactc taacatttga cactgaactt acagtgcaaa ctgtggcaga ccttaagcat
     1561 gccattcaaa gcaaatacaa gattgctatt caacaccagg tgctggtggt caatggagga
     1621 gaatgcatgg ctgcagatcg aagagtgtgt acctacagtg ctgggacgga tacaaatcca
     1681 atttttcttt ttaacaaaga aatgatctta tgtgatcgtc cacctgctat tcctaaaact
     1741 accttttcga cagaaaatga catggaaata aaagttgaag aatctcttat gatgcctgca
     1801 gtttttcata ctgttgcttc aaggacacag cttgcattgg aaatgtatga agttgccaag
     1861 aaactttgtt ctttttgtga aggtcttgta catgatgaac atcttcaaca ccaaggctgg
     1921 gctgcaatca tggccaacct ggaggactgt tcaaattcat accaaaagct acttttcaag
     1981 tttgaaagta tttattcaaa ttatctgcag tccatagaag acatcaagtt aaaacttact
     2041 catttaggaa ctgcagtttc agtaatggcc aagattccac tgttggagtg cctaaccaga
     2101 catagttaca gagaatgttt gggaagactg gattctttac ctgaacatga agactcagaa
     2161 aaagctgaga cgaaaagatc cactgaactg gtgctctctc ctgatatgcc tagaacaact
     2221 aacgaatctt tgttaacctc atttcccaag tcagtggaac atgtgtcccc agataccgca
     2281 gatgctgaaa gtggcaaaga aattagggaa tcttgtcaaa gtactgttca tcagcaagat
     2341 gaaactacga ttgacactaa agatggtgat ctgccctttt ttaatgtctc tttgttagac
     2401 tggataaatg ttcaagatag acctaatgat gtggaatctt tggtcaggaa gtgctttgat
     2461 tctatgagca ggcttgatcc aaggattatt cgaccattta tagcagaatg ccgtcaaact
     2521 attgccaaac ttgataatca gaatatgaaa gccattaaag gacttgaaga tcggctctac
     2581 gccctggacc agatgattgc tagctgtggc cgactggtga atgaacagaa agagcttgct
     2641 cagggatttt tagctaatca gaagagagct gaaaacttaa aggatgcatc tgtattacct
     2701 gatttatgcc tgagtcacgc aaatcagttg atgattatgt tgcaaaatca tagaaaactg
     2761 ttagatatta agcagaagtg taccactgcc aaacaagaac tagcaaataa cctacatgtc
     2821 agactgaagt ggtgttgctt tgtaatgctt catgctgatc aagatggaga gaagttacaa
     2881 gctttgctcc gcctcgtaat agagctgtta gaaagagtca aaattgttga agctcttagt
     2941 acagttcctc agatgtactg cttagctgtt gttgaggttg taagaagaaa aatgttcata
     3001 aaacactaca gggagtgggc tggtgcttta gtcaaagatg gaaagagatt atatgaagca
     3061 gaaaaatcaa aaagggaatc ctttgggaaa ttatttagga agtctttttt aagaaatcgt
     3121 ctgtttaggg gactggactc ctggccccct tccttttgta ctcaaaagcc tcgaaagttt
     3181 gactgtgaac ttccagatat ttcattaaaa gatttacagt ttctgcaatc attttgtcct
     3241 tcggaagttc agccattcct cagggttccc ttactttgtg actttgaacc tctacaccag
     3301 catgtacttg ctctacataa tttggtaaaa gcagcacaaa gtttggatga aatgtcacag
     3361 accattacag atctactgag tgaacaaaag gcatctgtga gtcagacatc cccacagtct
     3421 gcttcttcac caaggatgga aagtacagca ggaattacaa ctactacctc accgagaact
     3481 cctccaccac tgactgttca ggatccctta tgtcctgcag tttgtccctt agaagaatta
     3541 tctccagata gtattgatgc acatacgttt gattttgaaa ctattcccca tccaaacata
     3601 gaacagacta ttcaccaagt ttctttagac ttggattcat tagcagaaag tcctgaatca
     3661 gattttatgt ctgctgtgaa tgagtttgta atagaagaaa atttgtcgtc tcctaatcct
     3721 ataagtgatc cacaaagccc agaaatgatg gtggaatcac tttattcatc agttatcaat
     3781 gcgatagaca gtagacgaat gcaggataca aatgtatgtg gtaaggagga ttttggagat
     3841 catacttctc tgaatgtcca gttggaaaga tgtagagttg ttgcccaaga ctctcacttc
     3901 agtatacaaa ccattaagga agacctttgc cactttagaa catttgtaca aaaagaacag
     3961 tgtgacttct caaattcatt aaaatgtaca gcagtagaaa taagaaacat tattgaaaaa
     4021 gtaaaatgtt ctctggaaat aacactaaaa gaaaaacatc aaaaagaact actgtcttta
     4081 aaaaatgaat atgaaggtaa acttgacgga ctaataaagg aaactgaaga gaatgaaaac
     4141 aaaattaaaa aattgaaggg agagttagta tgccttgagg aggttttaca aaataaagat
     4201 aatgaatttg ctttggttaa acatgaaaaa gaagctgtaa tctgcctgca gaatgaaaag
     4261 gatcagaagt tgttagagat ggaaaatata atgcactctc aaaattgtga aattaaagaa
     4321 ctgaagcagt cacgagaaat agtgttagaa gacttaaaaa agctccatgt tgaaaatgat
     4381 gagaagttac agttattgag ggcagaactt cagtccttgg agcaaagtca tctaaaggaa
     4441 ttagaggaca cacttcaggt taggcacata caagagtttg agaaggttat gacagaccac
     4501 agagtttctt tggaggaatt aaaaaaggaa aaccaacaaa taattaatca aatacaagaa
     4561 tctcatgctg aaattatcca ggaaaaagaa aaacagttac aggaattaaa actcaaggtt
     4621 tctgatttgt cagacacgag atgcaagtta gaggttgaac ttgcgttgaa ggaagcagaa
     4681 actgatgaaa taaaaatttt gctggaagaa agcagagccc agcagaagga gaccttgaaa
     4741 tctcttcttg aacaagagac agaaaatttg agaacagaaa ttagtaaact caaccaaaag
     4801 attcaggata ataatgaaaa ttatcaggtg ggcttagcag agctaagaac tttaatgaca
     4861 attgaaaaag atcagtgtat ttccgagtta attagtagac atgaagaaga atctaatata
     4921 cttaaagctg aattaaacaa agtaacatct ttgcataacc aagcatttga aatagaaaaa
     4981 aacctaaaag aacaaataat tgaactgcag agtaaattgg attcagaatt gagtgctctt
     5041 gaaagacaaa aagatgaaaa aattacccaa caagaagaga aatacgaagc tattatccag
     5101 aaccttgaga aagacagaca aaaattggtc agcagccagg agcaagacag agaacagtta
     5161 attcagaagc ttaattgtga aaaagatgaa gctattcaga ctgccctaaa agaatttaaa
     5221 ttggagagag aagttgttga gaaagagtta ttagaaaaag ttaaacatct tgagaatcaa
     5281 atagcaaaaa gtcctgccat tgactctacc agaggagatt cttcaagctt agttgctgaa
     5341 cttcaagaaa agcttcagga agaaaaagct aagtttctag aacaacttga agagcaagaa
     5401 aaaagaaaga atgaagaaat gcaaaatgtt cgaacatctt tgattgcgga acaacagacc
     5461 aattttaaca ctgttttaac aagagagaaa atgagaaaag aaaacataat aaatgatctt
     5521 agtgataagt tgaaaagtac aatgcagcaa caagaacggg ataaagattt gatagagtca
     5581 ctttctgaag atcgagctcg tttgcttgag gaaaagaaaa agcttgaaga agaagtcagt
     5641 aagttgcgca gtagcagttt tgttccttca ccatatgtag ctacagcccc agaactttat
     5701 ggagcttgtg cacctgaact cccaggtgaa tcagatagat ccgctgtgga aacagcagat
     5761 gaaggaagag tggattcagc aatggagaca agcatgatgt ctgtacaaga aaatattcat
     5821 atgttgtctg aagaaaaaca gcggataatg ctgttagaac gaacattgca attgaaagaa
     5881 gaagaaaata aacggttaaa tcaaagactg atgtctcaga gcatgtcttc agtatcttca
     5941 aggcattctg aaaagatagc tattagagat tttcaggtgg gagatttggt actcatcatc
     6001 ctagacgaac gccatgacaa ttatgtgtta tttactgtta gtcctacttt atattttcta
     6061 cattcagagt ctctacctgc cctggatctc aaaccaggtg agggtgcttc aggtgcatct
     6121 agaagaccct gggtactcgg aaaagtaatg gaaaaagaat actgtcaagc caaaaaggca
     6181 caaaacagat ttaaagttcc tttggggaca aagttttaca gagtgaaagc cgtatcatgg
     6241 aataagaaag tataagaatt catcgataga tctgatatcg gtaccagtcg actctagagg
     6301 atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     6361 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     6421 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     6481 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     6541 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     6601 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     6661 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     6721 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     6781 ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg
     6841 gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg
     6901 ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt
     6961 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7021 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7081 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7141 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7201 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     7261 tttttggagg aattgatcag cttgggatct gatcaagaga caggatgagg atcgtttcgc
     7321 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     7381 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     7441 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     7501 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     7561 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     7621 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     7681 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     7741 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     7801 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     7861 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     7921 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     7981 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     8041 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     8101 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     8161 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     8221 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8281 cccaccccgg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc
     8341 tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc
     8401 cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc
     8461 cggacgggac gctcctgcgc ctgatacaga acgaattgct tgcaggcatc tcatgagtgt
     8521 gtcttcccgt tttccgcctg aggtcactgc gtggatggag cgctggcgcc tgctgcgcga
     8581 cggcgagctg ctcaccaccc actcgccaag ctggaaccgt aaaaaggccg cgttgctggc
     8641 gtttttccat aggctccgcc gatcataatc agccatacca catttgtaga ggttttactt
     8701 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     8761 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8821 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8881 gtatcttatc atgtctggat caattcccta tagtgagtcg tattaaattc gtaatcatgt
     8941 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9001 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9061 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     9181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    10261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    10321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
