LOCUS       sequence-220173-       13477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13477)
  AUTHORS   Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, 
            Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van 
            Oudenaarden A, Weinberg RA
  TITLE     Slug and Sox9 cooperatively determine the mammary stem cell state.
  JOURNAL   Cell. 2012 Mar 2;148(5):1015-28.
  PUBMED    22385965
REFERENCE   2  (bases 1 to 13477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-03-2"; volume: "148(5)"; pages: "1015-28"
COMMENT     Sequence Label: pLV-beta-catenin deltaN90
FEATURES             Location/Qualifiers
     source          1..13477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     385..404
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        694..712
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     694..711
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             737..917
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    937..970
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    1114..1702
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1220..1231
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1629..1649
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1717..2511)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDRLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1828..1847)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2438..2457
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    2562..3113
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2564..3130
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2723..2742)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2952..2969
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5375..5404)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    5522..5639
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(5687..6865)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5696..6634
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5719..5739)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(7248..7292)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7477..7710
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8203..8328
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8375..9008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9176..9310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9200..9219)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9254..9273
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9735..9755)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9885..9950
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(11084..11413)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11098..11233
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11398..11418
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(11488..11505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11659..12247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11739..11758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12418..13209,13210..13278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13041..13060
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13279..13383)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13451..13469
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccattcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
      241 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
      301 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
      361 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
      421 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctggc tagcgatgac
      481 cctgctgatt ggttcgctga ccatttccgg gtgcgggacg gcgttaccag aaactcagaa
      541 ggttcgtcca accaaaccga ctctgacggc agtttacgag agagatgata gggtctgctt
      601 cagtaagcca gatgctacac aattaggctt gtacatattg tcgttagaac gcggctacaa
      661 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaatacacgg
      721 aattaattct agatgctgct agagattttc cacactgact aaaagggtct gagggatctc
      781 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
      841 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
      901 tctaaccaga gagacccagt acaagcaaaa agcagcataa cttcgtataa tgtatgctat
      961 acgaagttat tcccatggtg aattgatccc atcttgtctt cgttgggagt gaattagccc
     1021 ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     1081 tggtcttaaa ggtacctggc catacgtacc gatgcgggga ggcggcccaa agggagatcc
     1141 gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc
     1201 gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca
     1261 gaatccaggt ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca
     1321 ccacggaatt gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg
     1381 cgatgagttc cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac
     1441 aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc
     1501 cacgttgcct gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca
     1561 aggaggagaa aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag
     1621 cgtatccaca tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa
     1681 ttttgtaatc cagaggttga ttatcatatg actagttcag aagaactcgt caagaaggcg
     1741 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     1801 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     1861 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     1921 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     1981 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     2041 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     2101 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     2161 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     2221 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     2281 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     2341 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     2401 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     2461 ctccacccaa gcggccggag aacctgcgtg caatctatct tgttcaatca tcagggttat
     2521 tgtctcatga gcggatacat atttgaagaa ttcggccatg gtattatcat cgtgtttttc
     2581 aaaggaaaac cacgtccccg tggttcgggg ggcctagacg ttttttaacc tcgactaaac
     2641 acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg
     2701 ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc tttccacaac
     2761 tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat cttatacacg
     2821 tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt
     2881 cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct tcacgacatt
     2941 caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa
     3001 gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg tggaaaataa
     3061 catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa cgttaggggg
     3121 ggggggcgga atttacccgg tgtcggcaga atgcttaatg aattacaact cgagcggccg
     3181 ccagtgtgat ggattcgagc cctctcagca actctacagg ccaatcacaa tgcaagttca
     3241 gacaatacag ctaaaggatg atttacaggt cagtatcaaa ccaggccagc tgattgctgt
     3301 cacctggagg cagcccatcc atgaggtcct gggcatgccc cagatctggc agcccatcaa
     3361 ctggatagtc agcaccaggg tggtggccac ccatctcatg ttccatcatg gggtccatac
     3421 ccaaggcatc ctggccatat ccaccagagt gaaaagaacg atagctagga tcatcctggc
     3481 gatatccaag gggttctccc tgggcaccaa tatcaagtcc aagatcagca gtctcattcc
     3541 aagccattgg ctctgttctg aagagagagc tggtcagctc aactgaaagc cgtttcttgt
     3601 aatcttgtgg cttgtcctca gacattcgga acaaaacagc agctgcatat gtcgccacac
     3661 cttcattcct agagtgaagt aactctgtca gaggagctgt ggctccctca gcttcaatag
     3721 cttctgcagc ttccttgtcc tgagcaagtt cacagaggac ccctgcagct actctttgga
     3781 tgttttcaat gggagaataa agcagctgca caaacaatgg aatggtattt agtcctctga
     3841 taacaattcg gttgtgaaca tcccgagcta ggatgtgaag ggctccggta caaccttcaa
     3901 ctatttcttc catgcggacc ccctccacaa attgctgctg tgtcccaccc atggacgtac
     3961 ggcgctgggt atcctgatgt gcacgaacaa gcaactgaac tagtcgtgga atggcaccct
     4021 gctcacgcaa aggtgcatga tttgcgggac aaagggcaag atttcgaatc aatccaacag
     4081 tagcctttat cagaggccag tgggatggtg ggtgtaagag cttaaccaca actggtagtc
     4141 catagtgaag gcgaactgca ttctgggcca tctctgcttc ttggtgtcgg ctggtcagat
     4201 gacgaagagc acagatggca ggctcagtga tgtcttccct gtcaccagcc cgaaggacag
     4261 tacgcacaag agcctctata ccacccactt ggcagaccat catcttgttc ttataattat
     4321 tgcaagtgag gttagaaaga attccagctg cacaggtgac cacatttata tcatctgaac
     4381 ccagaagctg aacaagagtc ccaaggagac cttccatccc ttcctgttta gttgcagcat
     4441 ctgaaagatt cctgagagtc caaagacagt tctgaacaag acgttgacct ggatctgtca
     4501 ggtgaagtcc taaagcttgc attccaccag cttctacaat agccggctta ttactagagc
     4561 agacagatag caccttcagc actctgcttg tggtccacag tagtttttcg taagtatagg
     4621 tcctcattat atttactaaa gcttggggtc caccactagc cagtatgatg agcttgcttt
     4681 cttggttgcc ataagctaaa atttgaaggc agtctgtcgt aatagccaag aatttaacat
     4741 ttgttttgtt gagcaaggca accattttct gcagcccacc agctaaacgc actgccattt
     4801 tagctccttc ttgatgtaat aaaaggttgt ggagagttgt aatggcataa aacaacacag
     4861 aatccactgg tgaaccaagc attttcacca gggcaggaat gcctccagac ttaaagatgg
     4921 ccagtaagcc ctcacgatga tgggaaaggt tatgcaaggt cccagcggta caacgagctg
     4981 tttctacatc atttgtattc tgcatggtac gtacaatagc agacaccatc tgaggagaac
     5041 gcatgatagc gtgtctggaa gcttcctttt tagaaagctg atggaccata actgcagcct
     5101 tattaaccac cacctggtcc tcgtcattta gcagttttgt cagttcaggg attgcacgtg
     5161 tggcaagttc tgcatcatct tgatagttaa tcaagtttac aactgcatgt ttcagcatct
     5221 gtgatggttc agccaaacgc tggacattag tgggatgagc agcatcaaac tgtgtagatg
     5281 ggatctgcat gccctcatct aatgtctcag ggaacatagc agctcgtacc ctctgagctc
     5341 gaatctgcag aattcgtcga catcgatgga tcctcaggtc ctcctcggag atcagcttct
     5401 gctccatggg attgacgcgt gtcttctatg gaggtcaaaa cagcgtggat tcaactatct
     5461 atcgcgtaag cttaggcctg gatcccgtag tttaaacctc gaggctagtc tcgtgatcga
     5521 taaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     5581 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaag
     5641 ttaaccgata ccgtcgagat taattaaatt taaatgtcga aattcctcac gacacctgaa
     5701 atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact
     5761 caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct
     5821 ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg
     5881 acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg
     5941 agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac
     6001 ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa
     6061 gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg
     6121 gggcgataca cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc
     6181 cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg
     6241 acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt
     6301 ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat
     6361 tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc
     6421 gcacgcggcg gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc
     6481 cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa
     6541 tcacgtactg cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga
     6601 ggccaggccc gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc
     6661 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
     6721 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
     6781 caccggttca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
     6841 ctgcccactg acgggcaccg gagccaattc ccactccttt caagacctag aaggtccatt
     6901 agctgcaaag attcctctct gtttaaaact ttatccatct ttgcaaagct tatcgtcgac
     6961 atcgatggtc gaggctagtc tcgtgatcga taccgtcgag atccgttcac taatcgaatg
     7021 gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc
     7081 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
     7141 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
     7201 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
     7261 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
     7321 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
     7381 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
     7441 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
     7501 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
     7561 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac
     7621 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct
     7681 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc
     7741 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat
     7801 ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc
     7861 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc
     7921 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt
     7981 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat
     8041 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc
     8101 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg
     8161 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc
     8221 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc
     8281 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt
     8341 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa
     8401 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     8461 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     8521 tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta
     8581 tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa
     8641 gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg
     8701 atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac
     8761 ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc
     8821 tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc
     8881 aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta
     8941 gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg agtgaattag
     9001 cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt taaaatgaaa
     9061 ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg gcagtggtaa
     9121 tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc cagttgatcc
     9181 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     9241 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     9301 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     9361 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta
     9421 gtcaaggcac tatacatcaa atattcctta ttaacccctt tacaaattaa aaagctaaag
     9481 gtacacaatt tttgagcata gttattaata gcagacactc tatgcctgtg tggagtaaga
     9541 aaaaacagta tgttatgatt ataactgtta tgcctactta taaaggttac agaatatttt
     9601 tccataattt tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa
     9661 gcaagagttc tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag
     9721 tccttggggt cttctacctt tctcttcttt tttggaggag tagaatgttg agagtcagca
     9781 gtagcctcat catcactaga tggcatttct tctgagcaaa acaggttttc ctcattaaag
     9841 gcattccacc actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca
     9901 attagaatca gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt
     9961 taaatctctg taggtagttt gtccaattat gtcacaccac agaagtaagg ttccttcaca
    10021 aagatccggg gcccactcat aaatccagtt gccgccacgg tagccaatca ccgtatcgta
    10081 taaatcatcg tcggtacgtt cggcatcgct catcacaata cgtgcctgga cgtcgaggat
    10141 ttcgcgtggg tcaatgccgc gccagatcca catcagacgg ttaatcatgc gataccagtg
    10201 agggatggtt ttaccatcaa gggccgactg cacaggcggt tgtgcgccgt gattaaagcg
    10261 gcggactagc gtcgaggttt caggatgttt aaagcggggt ttgaacaggg tttcgctcag
    10321 gtttgcctgt gtcatggatg cagcctccag aatacttact ggaaactatt gtaacccgcc
    10381 tgaagttaaa aagaacaacg cccggcagtg ccaggcgttg aaaagattag cgaccggaga
    10441 ttggcgggac gaatacgacg cccatatccc acggctgttc aatccaggta tcttgcggga
    10501 tatcaacaac atagtcatca accagcggac gaccagccgg ttttgcgaag atggtgacaa
    10561 agtgcgcttt tggatacatt tcacgaatcg caaccgcagt accaccggta tccaccaggt
    10621 catcaataac gatgaagcct tcgccatcgc cttctgcgcg tttcagcact ttaagctcgc
    10681 gctggttgtc gtgatcgtag ctggaaatac aaacggtatc gacatgacga atacccagtt
    10741 cacgcgccag taacgcaccc ggtacagacc gccacggctt acggcaataa tgcctttcca
    10801 ttgttcagaa ggcatcagtc ggcttgcgag tttacgtgca tggatctgca acatgtccca
    10861 ggtgacgatg tatttttcgc tcatgtgaag tgtcccagcc tgtttatcta cggcttaaaa
    10921 agtgttcgag gggaaaatag gttgcgcgag attatagaga tctggcgcac taaaaaccag
    10981 tatttcacat gagtccgcgt ctttttacgc actgcctctc cctgacgcgg gataaagtgg
    11041 tattctcaaa catatctcgc aagcctgtct tgtgtccaag ctttttgcaa aagcctaggc
    11101 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
    11161 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
    11221 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
    11281 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
    11341 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
    11401 ctaactgaca cacattccac agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    11461 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    11521 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    11581 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    11641 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    11701 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    11761 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    11821 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    11881 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    11941 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    12001 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    12061 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    12121 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    12181 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    12241 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    12301 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    12361 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    12421 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    12481 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    12541 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    12601 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    12661 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    12721 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    12781 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    12841 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    12901 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    12961 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    13021 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    13081 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    13141 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    13201 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    13261 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    13321 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    13381 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    13441 aacctataaa aataggcgta tcacgaggcc ctttcgt
//
