LOCUS       sequence-220191-        8306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene constitutive expression from a Pc promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Xu Y, Tao F, Ma C, Xu P
  TITLE     New constitutive vectors: useful genetic engineering tools for 
            biocatalysis.
  JOURNAL   Appl Environ Microbiol. 2013 Apr;79(8):2836-40. doi: 
            10.1128/AEM.03746-12. Epub 2013 Feb 15.
  PUBMED    23416993
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03746-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-04"; volume: "79"; issue: "8"; pages: "2836-40"
COMMENT     Sequence Label: pMMPc
FEATURES             Location/Qualifiers
     source          1..8306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..83)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      236..322
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      414..441
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        460..551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(552..620,621..1412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(770..789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1849..2243
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            2585..2672
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             3911..4882
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5393..6232
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             6219..7070
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
ORIGIN
        1 ttcccgggga tccgtcgacc tgcagccaag cttggctgtt ttggcggatg agagaagatt
       61 ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
      121 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
      181 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
      241 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      301 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
      361 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
      421 catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac
      481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      781 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1441 tttagattga tttctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg
     1501 tagaaagcca tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata
     1561 gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc
     1621 aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg
     1681 atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg
     1741 tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg
     1801 ttgctagaca ttgccagcca gtgcccgtca ctcccgtact aactgtcacg aacccctgca
     1861 ataactgtca cgcccccctg caataactgt cacgaacccc tgcaataact gtcacgcccc
     1921 caaacctgca aacccagcag gggcgggggc tggcggggtg ttggaaaaat ccatccatga
     1981 ttatctaaga ataatccact aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct
     2041 tgcccaatat gcccggccag aggccggata gctggtctat tcgctgcgct aggctacaca
     2101 ccgccccacc gctgcgcggc agggggaaag gcgggcaaag cccgctaaac cccacaccaa
     2161 accccgcaga aatacgctgg agcgctttta gccgctttag cggcctttcc ccctacccga
     2221 agggtggggg cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct
     2281 catccttctg gcgtggcggc agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg
     2341 catccattgc cgccatgagc cgatcctccg gccactcgct gctgttcacc ttggccaaaa
     2401 tcatggcccc caccagcacc ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc
     2461 ttgcccgctc ccgctgaatt tcggcattga ttcgcgctcg ttgttcttcg agcttggcca
     2521 gccgatccgc cgccttgttg ctccccttaa ccatcttgac accccattgt taatgtgctg
     2581 tctcgtaggc tatcatggag gcacagcggc ggcaatcccg accctacttt gtaggggagg
     2641 gcgcacttac cggtttctct tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc
     2701 tctccgaggg ccattgcatg gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat
     2761 ttatcacctt acggcgaaaa ccggcagcag gtcgggcggc caatcggcca gggccaaggc
     2821 cgactacatc cagcgcgaag gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga
     2881 atccgggcac atgccggagt tcgtcgagcg gcccgccgac tactgggatg ctgccgacct
     2941 gtatgaacgc gccaatgggc ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct
     3001 gaccctcgac cagcagaagg cgctggcgtc cgagttcgcc cagcacctga ccggtgccga
     3061 gcgcctgccg tatacgctgg ccatccatgc cggtggcggc gagaacccgc actgccacct
     3121 gatgatctcc gagcggatca atgacggcat cgagcggccc gccgctcagt ggttcaagcg
     3181 gtacaacggc aagaccccgg agaagggcgg ggcacagaag accgaagcgc tcaagcccaa
     3241 ggcatggctt gagcagaccc gcgaggcatg ggccgaccat gccaaccggg cattagagcg
     3301 ggctggccac gacgcccgca ttgaccacag aacacttgag gcgcagggca tcgagcgcct
     3361 gcccggtgtt cacctggggc cgaacgtggt ggagatggaa ggccggggca tccgcaccga
     3421 ccgggcagac gtggccctga acatcgacac cgccaacgcc cagatcatcg acttacagga
     3481 ataccgggag gcaatagacc atgaacgcaa tcgacagagt gaagaaatcc agaggcatca
     3541 acgagttagc ggagcagatc gaaccgctgg cccagagcat ggcgacactg gccgacgaag
     3601 cccggcaggt catgagccag accaagcagg ccagcgaggc gcaggcggcg gagtggctga
     3661 aagcccagcg ccagacaggg gcggcatggg tggagctggc caaagagttg cgggaggtag
     3721 ccgccgaggt gagcagcgcc gcgcagagcg cccggagcgc gtcgcggggg tggcactgga
     3781 agctatggct aaccgtgatg ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat
     3841 cgttgctctt gctcgacctg acgccactga caaccgagga cggctcgatc tggctgcgct
     3901 tggtggcccg atgaagaacg acaggacttt gcaggccata ggccgacagc tcaaggccat
     3961 gggctgtgag cgcttcgata tcggcgtcag ggacgcaccc accggccaga tgatgaaccg
     4021 ggaatggtca gccgccgaag tgctccagaa cacgccatgg ctcaagcgga tgaatgccca
     4081 gggcaatgac gtgtatatca ggcccgccga gcaggagcgg catggtctgg tgctggtgga
     4141 cgacctcagc gagtttgacc tggatgacat gaaagccgag ggccgggagc ctgccctggt
     4201 agtggaaacc agcccgaaga actatcaggc atgggtcaag gtggccgacg ccgcaggcgg
     4261 tgaacttcgg gggcagattg cccggacgct ggccagcgag tacgacgccg acccggccag
     4321 cgccgacagc cgccactatg gccgcttggc gggcttcacc aaccgcaagg acaagcacac
     4381 cacccgcgcc ggttatcagc cgtgggtgct gctgcgtgaa tccaagggca agaccgccac
     4441 cgctggcccg gcgctggtgc agcaggctgg ccagcagatc gagcaggccc agcggcagca
     4501 ggagaaggcc cgcaggctgg ccagcctcga actgcccgag cggcagctta gccgccaccg
     4561 gcgcacggcg ctggacgagt accgcagcga gatggccggg ctggtcaagc gcttcggtca
     4621 tgacctcagc aagtgcgact ttatcgccgc gcagaagctg gccagccggg gccgcagtgc
     4681 cgaggaaatc ggcaaggcca tggccgaggc cagcccagcg ctggcagagc gcaagcccgg
     4741 ccacgaagcg gattacatcg agcgcaccgt cagcaaggtc atgggtctgc ccagcgtcca
     4801 gcttgcgcgg gccgagctgg cacgggcacc ggcaccccgc cagcgaggca tggacagggg
     4861 cgggccagat ttcagcatgt agtgcttgcg ttggtactca cgcctgttat actatgagta
     4921 ctcacgcaca gaagggggtt ttatggaata cgaaaaaagc gcttcagggt cggtctacct
     4981 gatcaaaagt gacaagggct attggttgcc cggtggcttt ggttatacgt caaacaaggc
     5041 cgaggctggc cgcttttcag tcgctgatat ggccagcctt aaccttgacg gctgcacctt
     5101 gtccttgttc cgcgaagaca agcctttcgg ccccggcaag tttctcggtg actgatatga
     5161 aagaccaaaa ggacaagcag accggcgacc tgctggccag ccctgacgct gtacgccaag
     5221 cgcgatatgc cgagcgcatg aaggccaaag ggatgcgtca gcgcaagttc tggctgaccg
     5281 acgacgaata cgaggcgctg cgcgagtgcc tggaagaact cagagcggcg cagggcgggg
     5341 gtagtgaccc cgccagcgcc taaccaccaa ctgcctgcaa aggaggcaat caatggctac
     5401 ccataagcct atcaatattc tggaggcgtt cgcagcagcg ccgccaccgc tggactacgt
     5461 tttgcccaac atggtggccg gtacggtcgg ggcgctggtg tcgcccggtg gtgccggtaa
     5521 atccatgctg gccctgcaac tggccgcaca gattgcaggc gggccggatc tgctggaggt
     5581 gggcgaactg cccaccggcc cggtgatcta cctgcccgcc gaagacccgc ccaccgccat
     5641 tcatcaccgc ctgcacgccc ttggggcgca cctcagcgcc gaggaacggc aagccgtggc
     5701 tgacggcctg ctgatccagc cgctgatcgg cagcctgccc aacatcatgg ccccggagtg
     5761 gttcgacggc ctcaagcgcg ccgccgaggg ccgccgcctg atggtgctgg acacgctgcg
     5821 ccggttccac atcgaggaag aaaacgccag cggccccatg gcccaggtca tcggtcgcat
     5881 ggaggccatc gccgccgata ccgggtgctc tatcgtgttc ctgcaccatg ccagcaaggg
     5941 cgcggccatg atgggcgcag gcgaccagca gcaggccagc cggggcagct cggtactggt
     6001 cgataacatc cgctggcagt cctacctgtc gagcatgacc agcgccgagg ccgaggaatg
     6061 gggtgtggac gacgaccagc gccggttctt cgtccgcttc ggtgtgagca aggccaacta
     6121 tggcgcaccg ttcgctgatc ggtggttcag gcggcatgac ggcggggtgc tcaagcccgc
     6181 cgtgctggag aggcagcgca agagcaaggg ggtgccccgt ggtgaagcct aagaacaagc
     6241 acagcctcag ccacgtccgg cacgacccgg cgcactgtct ggcccccggc ctgttccgtg
     6301 ccctcaagcg gggcgagcgc aagcgcagca agctggacgt gacgtatgac tacggcgacg
     6361 gcaagcggat cgagttcagc ggcccggagc cgctgggcgc tgatgatctg cgcatcctgc
     6421 aagggctggt ggccatggct gggcctaatg gcctagtgct tggcccggaa cccaagaccg
     6481 aaggcggacg gcagctccgg ctgttcctgg aacccaagtg ggaggccgtc accgctgaat
     6541 gccatgtggt caaaggtagc tatcgggcgc tggcaaagga aatcggggca gaggtcgata
     6601 gtggtggggc gctcaagcac atacaggact gcatcgagcg cctttggaag gtatccatca
     6661 tcgcccagaa tggccgcaag cggcaggggt ttcggctgct gtcggagtac gccagcgacg
     6721 aggcggacgg gcgcctgtac gtggccctga accccttgat cgcgcaggcc gtcatgggtg
     6781 gcggccagca tgtgcgcatc agcatggacg aggtgcgggc gctggacagc gaaaccgccc
     6841 gcctgctgca ccagcggctg tgtggctgga tcgaccccgg caaaaccggc aaggcttcca
     6901 tagatacctt gtgcggctat gtctggccgt cagaggccag tggttcgacc atgcgcaagc
     6961 gccgcaagcg ggtgcgcgag gcgttgccgg agctggtcgc gctgggctgg acggtaaccg
     7021 agttcgcggc gggcaagtac gacatcaccc ggcccaaggc ggcaggctga ccccccccac
     7081 tctattgtaa acaagacatt tttatctttt atattcaatg gcttattttc ctgctaattg
     7141 gtaataccat gaaaaatacc atgctcagaa aaggcttaac aatattttga aaaattgcct
     7201 actgagcgct gccgcacagc tccataggcc gctttcctgg ctttgcttcc agatgtatgc
     7261 tcttctgctc ccgaacgcca gcaagacgta gcccagcgcg tcggccagct tgcaattcgc
     7321 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7381 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
     7441 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
     7501 ccctgagaga gttgcagcaa gcggtccacg tggtttgccc cagcaggcga aaatcctgtt
     7561 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
     7621 ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
     7681 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
     7741 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
     7801 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
     7861 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
     7921 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
     7981 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
     8041 catccagcgg atagttaatg atcagcccac tgacgcgttg ccgatacaag aacaacagcg
     8101 cgttgagcgc ctgccggtgg gtggccggcg ccacttgctt ctcggtggcg agcatggtca
     8161 gaaaaccctc gacttcagct tgccccattt cgcgcggatg tcgaaaccca ccatggctgc
     8221 gggccgtcca caacacaaat gccttggccc agtagacata agccttctcg gtctgtaggc
     8281 tgtaatgcag gtagcgaacc cgtgaa
//
