LOCUS       sequence-220199-       11659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of N-terminally tagged 
            eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged
            chromatin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling 
            AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato
            S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, 
            Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY,
            Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, 
            Lord CJ, Rottenberg S, Durocher D
  TITLE     The shieldin complex mediates 53BP1-dependent DNA repair.
  JOURNAL   Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. 
            Epub 2018 Jul 18.
  PUBMED    30022168
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0340-7"; journalName: "Nature"; date: "2018-08";
            volume: "560"; issue: "7716"; pages: "117-121"
COMMENT     Sequence Label: pCW-eGFP-FHA-SHLD2
FEATURES             Location/Qualifiers
     source          1..11659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..47
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(113..115,116..118,119..829)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(113..115,116..118,119..829)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(158..179)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(419..438)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     766..787
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4056..4080)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4098..4115
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4125..4635
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4554..4572
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4645..5241
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4645..4664)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5141..5161
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5242..5295
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5296..5913,5914..5919,5920..5955,5956..5958,5959..5994,
                     5995..5997,5998..6033,6034..6042)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6082..6102)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6500..6511)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6716..6949
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7021..7142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7058..7077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7112..7131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7182..7317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7317..7388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7389..7457,7458..8249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7607..7626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8420..9008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8909..8928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9162..9179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9296..9317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9332..9337,9338..9355,9356..9362)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9370..9386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9375..9397
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9394..9410
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9394..9410
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9429..9449
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9431..9449
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9477..9703
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9704..9884
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9931..10056
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10549..10782
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10967..11011
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11224..11341
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(11395..11659,1..50)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    11403..11421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11439..11457
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11474..11492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11510..11528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11546..11564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11581..11599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11617..11635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagaattg
       61 gctagcatca acaagtttgt acaaaaaagc aggcttcgaa ggagatagaa ccatggtgag
      121 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      181 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      241 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      301 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      361 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      421 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      481 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      541 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      601 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      661 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      721 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      781 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcgcgccagc
      841 cggagccggg gagcccggct tcttcgtcac aggagaccgc gccggtggcc ggagctggtg
      901 cctgcggcgg gtggggatga gcgccgggtg gctgctgctg gaagatgggt gcgaggtgac
      961 tgtaggacga ggatttggtg tcacatacca actggtatca aaaatctgcc ccctgatgat
     1021 ttctcgaaac cactgtgttt tgaagcagaa tcctgagggc caatggacaa ttatggacaa
     1081 caagagtcta aatggtgttt ggctgaacag agcgcgtctg gaacctttaa gggtctattc
     1141 cattcatcag ggagactaca tccaacttgg agtgcctctg gaaaataagg agaatgcgga
     1201 gtatgaatat gaagttactg aagaagactg ggagacaata tatccttgtc tttccccaaa
     1261 gaatgaccaa atgatagaaa aaaataagga attgagaact aaaaggaaat tcagtttgga
     1321 tgaagccgga gccggcgcgc caagtggagg atctcaagtc cacatttttt ggggtgctcc
     1381 aattgctcca ctgaaaatca cagtatcaga agacacagct tctttaatgt ctgttgctga
     1441 cccctggaaa aaaattcagc ttttatacag tcaacattct ttatatctga aggatgaaaa
     1501 acagcacaaa aatcttgaaa actataaagt cccagaatct attggttctc cagatcttag
     1561 tggtcatttc ttagcaaact gtatgaatag acatgttcat gtgaaagatg actttgtacg
     1621 ttctgtttct gaaacacaga atatagaatc ccagaagatt cactcctcta gactgagtga
     1681 tataactagc tctaatatgc aaatatgtgg atttaaaagc acagttccgc atttcaccga
     1741 agaagaaaag tatcaaaagc ttctcagtga aaataaaatt agagatgaac agcctaaaca
     1801 tcagccagat atatgtggta agaactttaa cacaaatttg tttcagttgg gccataaatg
     1861 tgcagctgtg ttggatttgg tttgtagtac tgaaaaaatt aatatagggc ctgaagtggt
     1921 acaaagagag tgtgtgccaa cagaatatca tgaaatacaa aaccagtgtt tgggattatt
     1981 ttcctcgaac gcagtagata agtcaaggtc tgaagcagca gttaggaagg tctcagacct
     2041 taaaatatca actgatacag aatttctcag tataattacc tccagccagg ttgctttttt
     2101 agctcaaaag aaagataaaa ggcggagtcc tgtaaataaa gggaatgtaa acatggagac
     2161 tgaaccaaag gcaagttacg gggagataag aatacctgaa gagaattcga ttcagcttga
     2221 tggttttaca gaagcatatg aaagtggaca aaaccaagca tattcccttg aactttttag
     2281 tcctgtttgt cctaaaacag aaaatagccg cattcacata aactctgata aaggtcttga
     2341 agaacataca ggatctcaag aacttttcag ttctgaagat gaactgccac caaatgagat
     2401 acgtattgag ttgtgtagct caggaatact gtgttcccaa ctaaatacct tccacaaaag
     2461 tgctattaaa agaagctgta cctctgaaga taaagtgggc cagtctgaag ctctatctag
     2521 agtccttcaa gtagctaaga aaatgaagtt gatttctaat ggaggagatt ctgctgtaga
     2581 aatggatcgg agaaatgtgt ctgaatttaa gagtattaaa aaaacatcat taataaaaaa
     2641 ctgtgattct aaaagccaga agtataattg tttagtcatg gtgctatctc catgccatgt
     2701 gaaggaaata aacataaaat tcggaccaaa ttctggctct aaagtgcctt tagcaacagt
     2761 tacagtaatt gatcaatcag aaactaagaa gaaggttttt ctgtggagga ctgcagcatt
     2821 ttgggcattt acagtgtttc ttggagatat aattttactc acagatgttg ttattcatga
     2881 ggaccaatgg attggcgaga cagtactaca atcaacattt agcagtcagt tattaaatct
     2941 tgggagttat tcatctattc agcctgaaga atattccagt gtagttagtg aagttgtact
     3001 tcaagactta ctggcatatg tgtcctcaaa acattcctac ctcagagatc ttcctccgag
     3061 gcagcctcag agggtgaaca gtatagactt tgtagaattg gagcaccttc aacctgatgt
     3121 attagtccac gcagtactaa gagttgttga tttcactata ctgacagagg cagtatacag
     3181 ttatagagga cagaagcaga aaaaagttat gttaacagtg gaacaggccc aagatcaaca
     3241 ttatgcgctt gtattatggg gtcctggagc agcctggtac cctcaacttc aaaggaaaaa
     3301 aggttatatt tgggaattta aatatctttt tgttcagtgc aattacacac tagaaaacct
     3361 agaattgcat acaacgcctt ggtcatcctg tgagtgcttg tttgatgatg atataagggc
     3421 aattacattt aaagcaaaat ttcaaaaaag tgcaccctcc tttgtgaaga tatcagactt
     3481 agcaacccac ctagaggata agtgttcagg agtggttctg attaaagccc agatttcaga
     3541 gctggcattt cctattacag catctcagaa gatagcgcta aatgctcaca gttctctgaa
     3601 gagtattttt tcttctcttc ccaacatcgt atatactggt tgtgcaaaat gtggattgga
     3661 actagaaaca gatgagaaca ggatctacaa acaatgtttt agctgcttgc catttactat
     3721 gaagaaaata tattataggc cagcgttaat gactgccatt gatggaagac atgatgtttg
     3781 tatccgtgta gaatcaaagc tgatagagaa gattcttctc aacatttctg cagactgcct
     3841 caacagagtg atagttcctt cctcagagat cacctatggg atggtcgtgg cagacctgtt
     3901 ccactccttg ttggcagtca gcgcagaacc ttgtgtatta aagattcaga gcctttttgt
     3961 gttagatgaa aacagctatc cattacaaca agatttctcc ctcctggatt tttatcctga
     4021 cattgtaaag catggagcca atgcccgtct ctaggaccca gctttcttgt acaaagtggt
     4081 ttagtaatga accggtccac caccaccacc accactaagg atccggggtt ggggttgcgc
     4141 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     4201 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     4261 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     4321 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     4381 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     4441 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     4501 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     4561 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     4621 gacctctctc cccagcaatt caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4681 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4741 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4801 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4861 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4921 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4981 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     5041 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5101 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5161 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5221 atgacccgca agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa
     5281 gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa
     5341 ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     5401 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     5461 ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
     5521 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     5581 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     5641 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     5701 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
     5761 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
     5821 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
     5881 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
     5941 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
     6001 gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc
     6061 aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6121 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6181 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6241 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6301 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6361 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6421 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6481 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6541 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6601 ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca
     6661 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6721 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6781 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6841 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6901 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6961 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7021 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7081 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7141 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     7201 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7261 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
     7321 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7381 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7441 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7561 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7621 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7681 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7741 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7801 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7861 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7921 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7981 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8041 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8101 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8161 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8221 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8581 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8641 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8761 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8821 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8881 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8941 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9061 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     9121 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     9181 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     9241 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9301 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9361 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     9421 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     9481 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     9541 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     9601 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     9661 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     9721 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9781 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9841 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     9901 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     9961 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
    10021 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
    10081 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
    10141 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
    10201 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
    10261 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
    10321 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
    10381 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
    10441 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
    10501 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
    10561 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
    10621 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
    10681 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
    10741 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    10801 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    10861 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    10921 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    10981 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    11041 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    11101 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    11161 ccacctccca accccgaggg gacaattctc gacctcgaga caaatggcag tattcatcca
    11221 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    11281 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    11341 tcgggtttat tacagggaca gcagagatcc actttggccg cgaatcgata tgtcgagttt
    11401 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
    11461 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
    11521 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
    11581 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
    11641 tgtcgaggta ggcgtgtac
//
