LOCUS       sequence-220204-       11659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of N-terminally tagged 
            eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment
            to damaged chromatin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Noordermeer SM, Adam S, Setiaputra D, Barazas M, Pettitt SJ, Ling 
            AK, Olivieri M, Alvarez-Quilon A, Moatti N, Zimmermann M, Annunziato
            S, Krastev DB, Song F, Brandsma I, Frankum J, Brough R, Sherker A, 
            Landry S, Szilard RK, Munro MM, McEwan A, Goullet de Rugy T, Lin ZY,
            Hart T, Moffat J, Gingras AC, Martin A, van Attikum H, Jonkers J, 
            Lord CJ, Rottenberg S, Durocher D
  TITLE     The shieldin complex mediates 53BP1-dependent DNA repair.
  JOURNAL   Nature. 2018 Aug;560(7716):117-121. doi: 10.1038/s41586-018-0340-7. 
            Epub 2018 Jul 18.
  PUBMED    30022168
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0340-7"; journalName: "Nature"; date: "2018-08";
            volume: "560"; issue: "7716"; pages: "117-121"
COMMENT     Sequence Label: pCW-eGFP-FHA-SHLD2-m1
FEATURES             Location/Qualifiers
     source          1..11659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(80..99)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     427..448
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3717..3741)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3759..3776
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3786..4296
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4215..4233
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4306..4902
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4306..4325)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4802..4822
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4903..4956
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4957..5574,5575..5580,5581..5616,5617..5619,5620..5655,
                     5656..5658,5659..5694,5695..5703)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5743..5763)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6161..6172)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6377..6610
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6682..6803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6719..6738)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6773..6792
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6843..6978
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6978..7049
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7050..7118,7119..7910)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7268..7287)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8081..8669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8570..8589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8823..8840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8957..8978
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8993..8998,8999..9016,9017..9023)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9031..9047
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9036..9058
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9055..9071
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9055..9071
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9090..9110
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9092..9110
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9138..9364
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9365..9545
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9592..9717
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10210..10443
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10628..10672
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10885..11002
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        11056..11370
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    11064..11082
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11100..11118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11135..11153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11171..11189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11207..11225
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11242..11260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11278..11296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11343..11367
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    11391..11415
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(11433..11435,11436..11438,11439..11659,1..490)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11433..11435,11436..11438,11439..11659,1..490)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11478..11499)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
       61 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      121 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      181 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      241 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      301 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      361 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      421 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      481 gctgtacaag ggcgcgccag ccggagccgg ggagcccggc ttcttcgtca caggagaccg
      541 cgccggtggc cggagctggt gcctgcggcg ggtggggatg agcgccgggt ggctgctgct
      601 ggaagatggg tgcgaggtga ctgtaggacg aggatttggt gtcacatacc aactggtatc
      661 aaaaatctgc cccctgatga tttctcgaaa ccactgtgtt ttgaagcaga atcctgaggg
      721 ccaatggaca attatggaca acaagagtct aaatggtgtt tggctgaaca gagcgcgtct
      781 ggaaccttta agggtctatt ccattcatca gggagactac atccaacttg gagtgcctct
      841 ggaaaataag gagaatgcgg agtatgaata tgaagttact gaagaagact gggagacaat
      901 atatccttgt ctttccccaa agaatgacca aatgatagaa aaaaataagg aattgagaac
      961 taaaaggaaa ttcagtttgg atgaagccgg agccggcgcg ccaagtggag gatctcaagt
     1021 ccacattttt tggggtgctc caattgctcc actgaaaatc acagtatcag aagacacagc
     1081 ttctttaatg tctgttgctg acccctggaa aaaaattcag cttttataca gtcaacattc
     1141 tttatatctg aaggatgaaa aacagcacaa aaatcttgaa aactataaag tcccagaatc
     1201 tattggttct ccagatctta gtggtcattt cttagcaaac tgtatgaata gacatgttca
     1261 tgtgaaagat gactttgtac gttctgtttc tgaaacacag aatatagaat cccagaagat
     1321 tcactcctct agactgagtg atataactag ctctaatatg caaatatgtg gatttaaaag
     1381 cacagttccg catttcaccg aagaagaaaa gtatcaaaag cttctcagtg aaaataaaat
     1441 tagagatgaa cagcctaaac atcagccaga tatatgtggt aagaacttta acacaaattt
     1501 gtttcagttg ggccataaat gtgcagctgt gttggatttg gtttgtagta ctgaaaaaat
     1561 taatataggg cctgaagtgg tacaaagaga gtgtgtgcca acagaatatc atgaaataca
     1621 aaaccagtgt ttgggattat tttcctcgaa cgcagtagat aagtcaaggt ctgaagcagc
     1681 agttaggaag gtctcagacc ttaaaatatc aactgataca gaatttctca gtataattac
     1741 ctccagccag gttgcttttt tagctcaaaa gaaagataaa aggcggagtc ctgtaaataa
     1801 agggaatgta aacatggaga ctgaaccaaa ggcaagttac ggggagataa gaatacctga
     1861 agagaattcg attcagcttg atggttttac agaagcatat gaaagtggac aaaaccaagc
     1921 atattccctt gaacttttta gtcctgtttg tcctaaaaca gaaaatagcc gcattcacat
     1981 aaactctgat aaaggtcttg aagaacatac aggatctcaa gaacttttca gttctgaaga
     2041 tgaactgcca ccaaatgaga tacgtattga gttgtgtagc tcaggaatac tgtgttccca
     2101 actaaatacc ttccacaaaa gtgctattaa aagaagctgt acctctgaag ataaagtggg
     2161 ccagtctgaa gctctatcta gagtccttca agtagctaag aaaatgaagt tgatttctaa
     2221 tggaggagat tctgctgtag aaatggatcg gagaaatgtg tctgaattta agagtattaa
     2281 aaaaacatca ttaataaaaa actgtgattc taaaagccag aagtataatt gtttagtcat
     2341 ggtgctatct ccatgccatg tgaaggaaat aaacataaaa ttcggaccaa attctggctc
     2401 taaagtgcct ttagcaacag ttacagtaat tgatcaatca gaaactaaga agaaggtttt
     2461 tctggcgagg actgcagcag ctgcggcatt tacagtgttt cttggagata taattttact
     2521 cacagatgtt gttattcatg aggaccaatg gattggcgag acagtactac aatcaacatt
     2581 tagcagtcag ttattaaatc ttgggagtta ttcatctatt cagcctgaag aatattccag
     2641 tgtagttagt gaagttgtac ttcaagactt actggcatat gtgtcctcaa aacattccta
     2701 cctcagagat cttcctccga ggcagcctca gagggtgaac agtatagact ttgtagaatt
     2761 ggagcacctt caacctgatg tattagtcca cgcagtacta agagttgttg atttcactat
     2821 actgacagag gcagtataca gttatagagg acagaagcag aaaaaagtta tgttaacagt
     2881 ggaacaggcc caagatcaac attatgcgct tgtattatgg ggtcctggag cagcctggta
     2941 ccctcaactt caaaggaaaa aaggttatat ttgggaattt aaatatcttt ttgttcagtg
     3001 caattacaca ctagaaaacc tagaattgca tacaacgcct tggtcatcct gtgagtgctt
     3061 gtttgatgat gatataaggg caattacatt taaagcaaaa tttcaaaaaa gtgcaccctc
     3121 ctttgtgaag atatcagact tagcaaccca cctagaggat aagtgttcag gagtggttct
     3181 gattaaagcc cagatttcag agctggcatt tcctattaca gcatctcaga agatagcgct
     3241 aaatgctcac agttctctga agagtatttt ttcttctctt cccaacatcg tatatactgg
     3301 ttgtgcaaaa tgtggattgg aactagaaac agatgagaac aggatctaca aacaatgttt
     3361 tagctgcttg ccatttacta tgaagaaaat atattatagg ccagcgttaa tgactgccat
     3421 tgatggaaga catgatgttt gtatccgtgt agaatcaaag ctgatagaga agattcttct
     3481 caacatttct gcagactgcc tcaacagagt gatagttcct tcctcagaga tcacctatgg
     3541 gatggtcgtg gcagacctgt tccactcctt gttggcagtc agcgcagaac cttgtgtatt
     3601 aaagattcag agcctttttg tgttagatga aaacagctat ccattacaac aagatttctc
     3661 cctcctggat ttttatcctg acattgtaaa gcatggagcc aatgcccgtc tctgagaccc
     3721 agctttcttg tacaaagtgg tttagtaatg aaccggtcca ccaccaccac caccactaag
     3781 gatccggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     3841 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     3901 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     3961 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     4021 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     4081 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     4141 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     4201 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     4261 tccctcgttg accgaatcac cgacctctct ccccagcaat tcaccatgac cgagtacaag
     4321 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     4381 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     4441 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     4501 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     4561 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     4621 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     4681 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     4741 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     4801 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     4861 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccgaaggtag aggttctctc
     4921 ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt ctagactgga caagagcaaa
     4981 gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg
     5041 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
     5101 cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc
     5161 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt
     5221 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
     5281 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
     5341 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa
     5401 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
     5461 cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc
     5521 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg
     5581 accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
     5641 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
     5701 taactaagta aggatcgatc caagatatcg tattcttaac tatgttgctc cttttacgct
     5761 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5821 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     5881 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     5941 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6001 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6061 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6121 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6181 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6241 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga
     6301 ctagaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6361 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     6421 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6481 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6541 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6601 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6661 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     6721 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6781 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     6841 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6901 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6961 gaggcttttt tggaggcctt tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7021 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7081 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7141 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7201 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7261 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7321 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7381 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7441 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7501 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7561 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7621 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7681 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7741 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7801 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7861 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7921 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7981 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8221 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8761 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8821 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8881 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8941 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9001 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9061 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     9121 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     9181 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     9241 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     9301 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     9361 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9421 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9481 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9541 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     9601 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     9661 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     9721 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9781 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9841 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9901 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9961 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    10021 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
    10081 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    10141 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    10201 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    10261 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    10321 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    10381 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    10441 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    10501 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    10561 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    10621 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    10681 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    10741 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    10801 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacaattct cgacctcgag
    10861 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
    10921 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
    10981 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
    11041 gcgaatcgat atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
    11101 ccctatcagt gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg
    11161 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
    11221 agagaacgta tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc
    11281 ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc
    11341 agagctcgtt tagtgaaccg tcagatcgcc tggagaattg gctagcatca acaagtttgt
    11401 acaaaaaagc aggcttcgaa ggagatagaa ccatggtgag caagggcgag gagctgttca
    11461 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
    11521 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
    11581 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
    11641 agtgcttcag ccgctaccc
//
