LOCUS       sequence-220242-       14704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, 
            notch-tdTomato; plus CMV-eGFP. Gateway expression vector for 
            lentivirus generation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14704)
  AUTHORS   Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
  TITLE     Luminescent and fluorescent triple reporter plasmid constructs for 
            Wnt, Hedgehog and Notch pathway.
  JOURNAL   PLoS One. 2019 Dec 20;14(12):e0226570. doi: 
            10.1371/journal.pone.0226570. eCollection 2019.
  PUBMED    31860685
REFERENCE   2  (bases 1 to 14704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-12-20"; volume: "14(12):e0226570. doi"; pages: " 
            10.1371/journal.pone.0226570. eCollection 2019"
COMMENT     Sequence Label: 
            pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato
FEATURES             Location/Qualifiers
     source          1..14704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    5..29
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        57..436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        437..640
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     591..611
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        685..703
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      739..748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(745..747,748..750,751..1464)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(745..747,748..750,751..1461)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    1505..1525
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1700..1731
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             1805..2755
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     protein_bind    complement(2841..2861)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             join(4261..4263,4264..4266,4267..4974)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     protein_bind    5157..5177
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        5382..5578
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5429..5564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5654..5663
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5660..7090
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(7111..7127)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7112..7128)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(7139..7163)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7194..7782
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7247..7267)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7665..7676)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7796..8295
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7831..7854)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8228..8247
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8337..9131
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8391..8410)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9001..9020
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9253..9486
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9558..9679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9595..9614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9649..9668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9719..9854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9875..9893)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9903..9920)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9903..9919)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9912..9934)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10061..10516
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10148..10167)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10358..10379
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10542..10646
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10647..10715,10716..11507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10865..10884)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11678..12266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12167..12186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12420..12437
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12554..12575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12590..12595,12596..12613,12614..12620)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12628..12644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12633..12655
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12652..12668
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12652..12668
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12687..12707
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12689..12707
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12735..12961
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12962..13142
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13189..13314
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13807..14040
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14225..14269
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14536..14652
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atcaacaagt ttgtacaaaa aagcaggctg cgatgtacgg gccagatata cgcgttgaca
       61 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      121 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      181 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      241 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      301 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      361 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      421 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      481 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      541 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      601 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      661 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta
      721 ccgagctcgg atcctaaagc caccatggtg agcaagggcg aggagctgtt caccggggtg
      781 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      841 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      901 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      961 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1021 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1081 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1141 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1201 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1261 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1321 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1381 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1441 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gagctcgagc atgcatctag
     1501 aggacaactt tgtatacaaa agttgggaga cccaagcttg gtaccgagct cttacgcgag
     1561 atcaaagggg gtaagatcaa agggggtaag atcaaagggg cgcgagatca aagggggtaa
     1621 gatcaaaggg ggtaagatca aagggggtaa gatcaaaggg gcgcgcccgc gtgctagccc
     1681 gggctcgaga tctagactct agagggtata taatggaagc tcgaattcca gcttggcatt
     1741 ccggtactgt tggtaaaaag cttggcattc cggtactgtt ggtaaagcca ccatcagctg
     1801 caccatggcg gaaggatccg tcgccaggca gcctgacctc ttgacctgcg acgatgagcc
     1861 gatccatatc cccggtgcca tccaaccgca tggactgctg ctcgccctcg ccgccgacat
     1921 gacgatcgtt gccggcagcg acaaccttcc cgaactcacc ggactggcga tcggcgccct
     1981 gatcggccgc tctgcggccg atgtcttcga ctcggagacg cacaaccgtc tgacgatcgc
     2041 cttggccgag cccggggcgg ccgtcggagc accgatcact gtcggcttca cgatgcgaaa
     2101 ggacgcaggc ttcatcggct cctggcatcg ccatgatcag ctcatcttcc tcgagctcga
     2161 gcctccccag cgggacgtcg ccgagccgca ggcgttcttc cgccgcacca acagcgccat
     2221 ccgccgcctg caggccgccg aaaccttgga aagcgcctgc gccgccgcgg cgcaagaggt
     2281 gcggaagatt accggcttcg atcgggtgat gatctatcgc ttcgcctccg acttcagcgg
     2341 cgaagtgatc gcagaggatc ggtgcgccga ggtcgagtca aaactaggcc tgcactatcc
     2401 tgcctcaacc gtgccggcgc aggcccgtcg gctctatacc atcaacccgg tacggatcat
     2461 tcccgatatc aattatcggc cggtgccggt caccccagac ctcaatccgg tcaccgggcg
     2521 gccgattgat cttagcttcg ccatcctgcg cagcgtctcg cccgtccatc tggaattcat
     2581 gcgcaacata ggcatgcacg gcacgatgtc gatctcgatt ttgcgcggcg agcgactgtg
     2641 gggattgatc gtttgccatc accgaacgcc gtactacgtc gatctcgatg gccgccaagc
     2701 ctgcgagcta gtcgcccagg ttctggcctg gcagatcggc gtgatggaag agtgagtcac
     2761 tagtaacggc cgccagtgtg ctggaattct gcagatatcc atcacactgg cggccgctcg
     2821 agcatgcatc tagaggcacc caacttttct atacaaagtt gggagaccca agcttggtac
     2881 cgagctctta cgcgtgctag agcttgcatg cctgcaggtc gacacactgg cgcactatcc
     2941 agcacgctgt cattattggt gcaggcctgg gaatgcgagc cggacctccc agtatttgct
     3001 gcagcttgag gccaaggtgc ctctgaaagg cttttctttg aggaatttca gaaattgttt
     3061 aagcgcttaa aaaaatatta catttccccc cgtgtctacc gcgaggcaaa tgttcgtgtg
     3121 tgtgcgtgcg cgcgcgtgtg tgcgcgcaaa ggcatcccac aaaaagaaga cactgttgaa
     3181 aagaaaagga ataagagaga aaggaggggg agcattcctt aatggaagta ttgcatgcga
     3241 gaaggttggc gctctcagag gctgccccga agccgaggat gcacacactg ggttgcctac
     3301 ctgggtggtc tctctacttt ggtgagctgt tgctaagcag ctaataatag tcatgtttgc
     3361 tgagtaattt ctgccctccg cgagccaatc gcgtcgcaga aacccaagcc atctgacagc
     3421 cccgggggcg ggagctccag cctttttctc tttcgctcgc tctctccccc tccaccccct
     3481 cccctcccct cctcctcttc tttctccaaa tggagagagt tctttttttt cttcttccat
     3541 ctcatctatt gaatcaagga gctgctgcgg ccgctgcccg ctgcacacac acagagcgga
     3601 gtccccaggt cccgggagcg agagaggcgc gctgcacggg gacagaatgg tccagcgggt
     3661 tgccgaggag cacaagaaag cagagtccgg gaccgagcag ccaccgcgaa cccagcagcc
     3721 agagcccgag cagcccgagc agcagctcct ggagccgcca ccgccagcag cagccaccgc
     3781 gggagcagcg gcagctgcgg ctgcccgggc ccggcagcgc tgagacccgc agggcaccct
     3841 cggaccccgc ggcggcggcg gctctgcgct tgccctccgc ggccgctcgg gcgacccggg
     3901 aggcgccgag agagccagcg cggccggcgg gagcagcggg gattcgctgg ctctttctgc
     3961 agtgaagggg tcgcggcgcg gggcgggggg cgggtagggg gagttgggga ccgcaaggag
     4021 tgccgcggaa gcgcccgaag gacaggctcg ctcggcgcgc cggctctcgc tcttccgcga
     4081 actggatgtg ggcagcggcg gccgcagaga cctcgggacc cccgcgcaat gtggcaatgg
     4141 aaggcgcagg gtctgactcc ccggcagcgg ccgcggccgc agcggcagca gcgcccgccg
     4201 tgtgagcagc agcagcggct ggtctgtcaa ccggagcgga tctgcgatct aagtaagctt
     4261 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4321 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4381 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4441 ctcgtgacca ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     4501 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4561 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4621 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4681 aagctggagt acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac
     4741 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     4801 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4861 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4921 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacagtgag
     4981 ctgcagaaca agctggaaga gttggatctg gactcgtaca agtaaagcgg ccgcgactct
     5041 agatcataat cagccatacc acatttgtag aggatccact agtaacggcc gccagtgtgc
     5101 tggaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct agaggacaac
     5161 tttgtataat aaagttgctg tggaatgtgt gtccatatga taaccgtatt accgccatgc
     5221 attagttatt aatcgagatc tggtgtaaac acgccgtggg aaaaaattta tggatctgga
     5281 gtaacacgcc gtgggaaaaa atttatggat ctggagtaac acgccgtggg aaaaaattta
     5341 tggatctgga gtaacacgcc gtgggaaaaa atttatggat ctgcatctca attagtcagc
     5401 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5461 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5521 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5581 gctggctagc gctaccggac tcagatctcg agctcaagct tcgaattcgc caccatgcca
     5641 gagccaggat cccgccacca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg
     5701 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     5761 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     5821 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt
     5881 gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg
     5941 ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct
     6001 gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg
     6061 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     6121 cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta
     6181 cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta
     6241 ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     6301 gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg
     6361 cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt
     6421 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
     6481 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     6541 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
     6601 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
     6661 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     6721 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     6781 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     6841 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     6901 ccgctacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     6961 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     7021 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct
     7081 gtacaagtaa gaattcgata tcaagcttat cgataccgtc gacctcgagc ctctagatac
     7141 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gacaatcaac
     7201 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7261 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7321 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7381 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7441 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7501 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7561 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     7621 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7681 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7741 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctaccgggta
     7801 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     7861 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     7921 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     7981 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     8041 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     8101 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     8161 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     8221 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     8281 tctccgggcc tttcgacctg cagcccaagc ttaccacact cctgcaggcg tttcgcatga
     8341 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     8401 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     8461 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     8521 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     8581 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     8641 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     8701 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     8761 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     8821 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     8881 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     8941 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     9001 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     9061 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     9121 agttcttctg agcgggactc tggggttcga aatgaccgac gcgtatgcat ctcgagggcc
     9181 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     9241 aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt
     9301 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9361 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9421 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9481 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     9541 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9601 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9661 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     9721 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9781 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9841 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     9901 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     9961 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    10021 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    10081 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    10141 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    10201 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    10261 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    10321 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    10381 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10441 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10501 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10561 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10621 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10681 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10741 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10801 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10861 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10921 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10981 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    11041 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    11101 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    11161 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    11221 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    11281 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    11341 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    11401 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    11461 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11521 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    11581 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    11641 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11701 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11761 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11821 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11881 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11941 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    12001 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    12061 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    12121 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    12181 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    12241 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    12301 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    12361 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    12421 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    12481 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    12541 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    12601 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    12661 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    12721 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
    12781 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
    12841 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
    12901 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
    12961 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    13021 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    13081 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    13141 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    13201 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    13261 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    13321 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    13381 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    13441 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    13501 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    13561 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    13621 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    13681 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    13741 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    13801 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    13861 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    13921 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    13981 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    14041 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    14101 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    14161 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    14221 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    14281 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    14341 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    14401 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
    14461 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
    14521 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
    14581 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
    14641 attcaaaatt ttatcgtcca gtgtggtgga attgagtatt ccagtgtggt ggaattctgc
    14701 agat
//
