LOCUS       sequence-22025-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Choi KM, McMahon LP, Lawrence JC
  TITLE     Two motifs in the translational repressor PHAS-I required for 
            efficient phosphorylation by mammalian target of rapamycin and for 
            recognition by raptor.
  JOURNAL   J Biol Chem. 2003 May 30. 278(22):19667-73.
  PUBMED    12665511
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 May 30. 278(22):19667-73."
COMMENT     Sequence Label: pET14b PHAS-I F113A
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(508..526)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      522..569
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgtttaactt taagaaggag atataccatg gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gtgccgcgcg gcagccatat gctcgaggat cctatgtcgg cgggcagcag
      121 ttgcagccag actcccagcc gggctatccc cactcgccgc gtagccctcg gcgacggcgt
      181 gcagctcccg cccggggact acagcaccac ccccggcggc acgctcttca gcaccacccc
      241 gggaggaacc agaatcatct atgaccggaa attcctgatg gagtgtcgga actcgcctgt
      301 ggccaaaaca cccccaaagg acctgccaac cattccaggg gtcactagcc ctaccagcga
      361 tgagcctccc atgcaggcca gccagagcca tctgcacagc agcccggaag ataagcgggc
      421 aggtggtgaa gagtcacagg ctgagatgga catttaagga tccggctgct aacaaagccc
      481 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      541 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac
      601 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
      661 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
      721 tgcatcaacg catatagcgc tagcagcacg ccatagtgac
//
