LOCUS       sequence-220254-        4469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFPnovo2_unc-54 utr vector for expression in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Hendi A, Mizumoto K
  TITLE     GFPnovo2, a brighter GFP variant for in vivo labeling in C. elegans.
  JOURNAL   MicroPubl Biol. 2018 Sep 6;2018:10.17912/49YB-7K39. doi: 
            10.17912/49YB-7K39.
  PUBMED    32550394
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2018-09-6"; volume: "2018:10.17912/49YB-7K39. doi"; 
            pages: " 10.17912/49YB-7K39"
COMMENT     Sequence Label: pSM-GFPnovo2
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(218..246)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(1988..2010)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2110..2129
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2211..2229)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2297..2401
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2402..2470,2471..3262)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2620..2639)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3433..4021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3922..3941
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4175..4192
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4309..4330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4345..4350,4351..4368,4369..4375)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4383..4399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4388..4410
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccactgact
       61 gggcgcgcct ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
      121 atactaacat aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt
      181 agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      241 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      301 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt
      361 aagtttaaac atatatatac taactaaccc tgattattta aattttcagc caacacttgt
      421 cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacggca
      481 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
      541 agatgacggg aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat
      601 atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      661 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      721 cttcaactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagctgt
      781 aagtttaaac atgattttac taactaacta atctgattta aattttcaga acttcaaaat
      841 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
      901 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgaccacac aatctgccct
      961 ttcgaaagat cccaacgaaa agagagacca catggtcctt gttgagtttg taacagctgc
     1021 tgggattaca catggcatgg atgaactata caaatagcat tcgtagaatt ccaactgagc
     1081 gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca
     1141 gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg
     1201 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     1261 cctgtgctcc caccccctat ttttgttatt atcaaaaaaa cttcttctta atttctttgt
     1321 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     1381 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     1441 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttttactt ctgataaatt
     1501 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     1561 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     1621 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     1681 aattaatttt cctgcttttg ctttttgggg gtttccccta ttgtttgtca agagtttcga
     1741 ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg
     1801 aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg
     1861 agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat
     1921 aactgtttcc tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg
     1981 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     2041 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     2101 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     2161 cgcacagatg cgtaaggaga aaataccgca tcaggcggcc ttaattaagg gcctcgtgat
     2221 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     2281 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2341 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2401 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2461 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2521 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2581 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2641 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     2701 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2761 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2821 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2881 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2941 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3001 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3061 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3121 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3181 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3241 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3301 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3361 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3481 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3541 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3601 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3661 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3721 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3781 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     3841 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3901 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3961 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4021 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4081 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4141 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4201 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     4261 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     4321 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     4381 aattgtgagc ggataacaat ttcacacagg aaacagctgg gggtgggggg ggggttgggg
     4441 gtggggtgtt gttggggtgt gttttgtgt
//
