LOCUS       sequence-22026-e         605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler 
            GR, Copeland TD, Kalkum M, Ge K
  TITLE     PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 
            methyltransferase complex.
  JOURNAL   J Biol Chem. 2007 Jul 13. 282(28):20395-406.
  PUBMED    17500065
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jul 13. 282(28):20395-406."
COMMENT     Sequence Label: pcDNA3 Flag RbBP5
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggtaccg agctcggatc ctggccacca tggactacaa
      121 ggacgacgat gacaagatga acctcgagtt gctggagtcc tttgggcaga actatccaga
      181 ggaagctgat ggaactttgg attgtatcag catggctttg acttgcacct ttaacaggtg
      241 gggcacactg cttgcagttg gctgtaatga tggccgaatt gtcatctggg atttcttgac
      301 aagaggcatt gctaaaataa ttagtgcaca catccatcca gtgtgttctt tatgctggag
      361 ccgagatggt cataaactcg tgagtgcttc cactgataac atagtgtcac agtgggatgt
      421 tctttcaggc gactgtgacc agaggtttcg attcccttca cccatcttaa aagtccaata
      481 tcatccacga gatcagaaca aggttctcgt gtgtcccatg aaatctgctc ctgtcatgtt
      541 gaccctttca gattccaaac atgttgttct gccagtggac gatgactccg atttgaacgt
      601 tgtgg
//
