LOCUS       sequence-22030-e         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler 
            GR, Copeland TD, Kalkum M, Ge K
  TITLE     PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 
            methyltransferase complex.
  JOURNAL   J Biol Chem. 2007 Jul 13. 282(28):20395-406.
  PUBMED    17500065
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jul 13. 282(28):20395-406."
COMMENT     Sequence Label: pcDNA3 Flag hDPY30
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(443..461)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(444..461)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(481..498)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    487..598
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 ttggtaccga gctcggatcc tggccaccat ggactacaag gacgacgatg acaagatgga
      121 gccagagcag atgctggagg gacaaacgca ggttgcagaa aatcctcact ctgagtacgg
      181 tctcacagac aacgttgaga gaatagtaga aaatgagaag attaatgcag aaaagtcatc
      241 aaagcagaag gtagatctcc agtctttgcc aactcgtgcc tacctggatc agacagttgt
      301 gcctatctta ttacagggac ttgctgtgct tgcaaaggaa agaccaccaa atcccattga
      361 atttctagca tcttatcttt taaaaaacaa ggcacagttt gaagatcgaa actgactcga
      421 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
      481 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      541 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      601 attgtctgag taggtgtcat t
//
