LOCUS       sequence-22032-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler 
            GR, Copeland TD, Kalkum M, Ge K
  TITLE     PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 
            methyltransferase complex.
  JOURNAL   J Biol Chem. 2007 Jul 13. 282(28):20395-406.
  PUBMED    17500065
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jul 13. 282(28):20395-406."
COMMENT     Sequence Label: pGEX-5X1 Flag hDPY30
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..23
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(431..453)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(598..616)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatcggatct gatcgaaggt cgtgggatcc tggccaccat ggactacaag gacgacgatg
       61 acaagatgga gccagagcag atgctggagg gacaaacgca ggttgcagaa aatcctcact
      121 ctgagtacgg tctcacagac aacgttgaga gaatagtaga aaatgagaag attaatgcag
      181 aaaagtcatc aaagcagaag gtagatctcc agtctttgcc aactcgtgcc tacctggatc
      241 agacagttgt gcctatctta ttacagggac ttgctgtgct tgcaaaggaa agaccaccaa
      301 atcccattga atttctagca tcttatcttt taaaaaacaa ggcacagttt gaagatcgaa
      361 actgactcga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg
      421 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
      481 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
      541 cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc
      601 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      661 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaa
//
