LOCUS       sequence-220331-       12393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of the S. epidermidis Cas10·Csm complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Hatoum-Aslan A, Samai P, Maniv I, Jiang W, Marraffini LA
  TITLE     A ruler protein in a complex for antiviral defense determines the 
            length of small interfering CRISPR RNAs.
  JOURNAL   J Biol Chem. 2013 Sep 27;288(39):27888-97. doi: 
            10.1074/jbc.M113.499244. Epub 2013 Aug 9.
  PUBMED    23935102
REFERENCE   2  (bases 1 to 12393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.499244"; journalName: "J Biol Chem"; date: 
            "2013-09-27- 27"; volume: "288"; issue: "39"; pages: "27888-97"
COMMENT     Sequence Label: pPS22 (pET23a-Cas10/Csm/csm2H6N)
FEATURES             Location/Qualifiers
     source          1..12393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        493..597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(598..666,667..1458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(816..835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1629..2217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2118..2137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2371..2388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2403..2542
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2468..2487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2628..2650
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2644..2835)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3344..3363
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3344..3362
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7238..7255
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(12307..12325)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12321..12368
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1141 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     1321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     1801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2401 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca
     2461 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     2521 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     2581 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     2701 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     2761 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     2821 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     2881 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     2941 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
     3001 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
     3061 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     3121 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     3181 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     3241 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
     3301 tggccaggac ccaacgctgc ccgagatctc gatcccgcga aattaatacg actcactata
     3361 gggagaccac aacggtttcc ctctagaagt gatggcattt gttaaagtat cggatcgata
     3421 cccaccccga agaaaagggg acgagaacac gtatgccgaa gtatataaat catcagtaca
     3481 aaggatcgat acccaccccg aagaaaaggg gacgagaact agtaataatt gtcatttgca
     3541 tacgttacat cgatgatcga tacccacccc gaagaaaagg ggacgagaac tagtacggtc
     3601 gtgaacattt tttcttgatt ctctgatcga tagccacccc gaagaaaagg gggcagagtg
     3661 taaatctaac aacactctaa aaaattgtag attttgaata aaatacgcat tgattaattt
     3721 ctaggggatg gaaaaatgaa agatgttatt tatgtagaaa atcattactt tgttactgcg
     3781 aaggaaaata gcattaaatt tagaaatgtc atagataaaa gtgaaaaatt ttatttgttt
     3841 gaagaaattg aagctattat ttttgatcat tataaaagct atttttcaca taaattggta
     3901 attaaatgta tagagaatga tattgcgatt atattttgtg acaaaaagca ttctccagtt
     3961 acacagctca tttcttcata tggcatggtt aatcgtctta aaagaattca aagtcaattt
     4021 cagttatcgg gaagaacaaa agatagaatt tggaaaaaga ttgttataaa taaaattttt
     4081 aaccaaacac gatgtcttga aaataattta cataatgaaa atgttaagct gatgttaggt
     4141 tttgcaaaag aagtgagttc aggagacaaa agtaataaag aagcacatgc tactcgtatt
     4201 tattttaaag atttatttgg caaacaattt aaacgtggac gctataatga tgttatcaat
     4261 tcaggattga attatggtta ttcaattctc agatctttta tcaacaaaga gttagctatt
     4321 catggatttg aaatgagcct aggtattaaa catcagtcaa aagaaaatcc atttaattta
     4381 gcagatgata tcattgaagt ttttcgtcct tttgtagata atatagtgta cgagatagtt
     4441 tttaagaaaa atattgatac atttgatata aacgaaaaga aattattatt aaatgttttg
     4501 tatgaaaggt gcattataga taaaaaagta gtgcgattac ttgatagtgt gaagatagtt
     4561 attcaatcgc taattagatg ttatgaagaa aatacaccta cttatttatt actacctaag
     4621 atgatagagg tgggaaacta atgtatttgt tagttagttt tgatttacct cgagatacta
     4681 aactagaacg tcgaattgct agtaaatatc gtcttagatt aatagaactt ggatttagta
     4741 tgaagcaatt tagtttatac gagagatatg tcagtaatgt tcaaaagaaa gataaaatct
     4801 tagaaatatt aaagcaggaa atacctgata ctggaagtat taccttatac atattgcctg
     4861 atgaagtgaa tagtaatcag attactatat taggaaaaga agtaaagctt gctgtcaaca
     4921 aagaaccaaa actaatcttt atataaatgg aggttaaaat gaataaaaaa aatatattaa
     4981 tgtatggctc tttattacat gatataggga aaattatata tcgaagtggt gatcatacat
     5041 tttcaagagg tacgcattca aaattaggtc atcaattttt gtcccaattt tcagaattta
     5101 aagacaacga agtgcttgat aacgttgctt atcatcatta caaagaactc gcaaaagcta
     5161 atttagataa tgataataca gcttatatta cctatattgc ggataatatt gcgagtggta
     5221 ttgatagaag agatattata gaagaaggcg atgaagaata cgaaaaacaa ctatttaatt
     5281 ttgataaata tacaccgcta tatagtgtgt ttaatattgt gaattctgaa aaattgaaac
     5341 aaacaaacgg gaagtttaaa ttttctaatg aaagtaatat tgaatatcct aaaactgaaa
     5401 acattcaata ttcaagtgga aattatacaa cattaatgaa agatatgagt catgatttag
     5461 agcacaaatt aagtattaaa gaaggtacat ttccttcatt attacaatgg acggaaagtc
     5521 tatggcaata tgtacctagt tcgacaaata aaaaccaatt aattgatatt tctctttatg
     5581 atcatagtcg tattacatgt gccatcgcca gttgtatatt tgattattta aatgaaaata
     5641 acatacataa ttacaaagat gaattgttct caaagtatga aaataccaaa tcattttatc
     5701 aaaaagaagc ttttttacta cttagtatgg atatgagtgg tattcaagat tttatttaca
     5761 atataagcgg ttctaaagca ttaaagagtc taagatctcg tagtttttat ttagaactca
     5821 tgcttgaagt aatcgttgat caattattag aaagattaga attagcacga gcaaatcttt
     5881 tgtatacagg tggtggccat gcttatttat tagtgtctaa tactgataaa gtgaagaaaa
     5941 aaataactca atttaataat gaattaaaaa aatggtttat gtcagaattt actacagatc
     6001 tttcattatc aatggctttt gaaaaatgta gtggcgatga tttaatgaat acaagtggta
     6061 attatagaac tatttggcgt aatgttagca gcaaactttc tgatattaaa gcgcataaat
     6121 attccgcgga agatatatta aaattaaatc attttcattc gtatggagat cgggaatgta
     6181 aagaatgttt aagaagtgac atagatatta atgatgatgg actatgtagt atatgtgaag
     6241 gaattattaa tatatcaaat gatttaagag ataaatcatt ctttgtactg tcagaaactg
     6301 gaaaattaaa aatgccattc aataaattta tatcggttat tgattatgaa gaggcagaaa
     6361 tgttagtaca aaataataat caagttcgta tttacagtaa aaataaacca tatataggca
     6421 taggaatatc aacaaattta tggatgtgtg attacgacta tgctagtcaa aatcaagata
     6481 tgagagaaaa aggtattgga agttatgtag atagagaaga aggggttaag cgtttaggcg
     6541 tggtacgtgc cgatatagat aatctcggtg ctacatttat atctggaatt ccagaaaaat
     6601 ataattcaat ttcaagaaca gctacattgt ctcgtcaatt atcattattt tttaaatacg
     6661 aattaaatca tttattagaa aattatcaaa ttactgctat atattcaggc ggtgacgatt
     6721 tatttttaat cggtgcatgg gatgacatta tagaagcaag catttatata aatgacaaat
     6781 ttaaagagtt tactcttgat aaactaacat tgtctgccgg ggttggaatg tttagtggta
     6841 agtacccagt ttctaaaatg gcttttgaga caggacgact tgaagaagcg gctaagactg
     6901 gtgaaaaaaa tcagatatct ctttggttac aagaaaaagt atataactgg gatgagttta
     6961 aaaagaatat cttagaagaa aaacttctcg ttttacaaca ggggttttct caaacagatg
     7021 aacacgggaa agccttcatt tataaaatgc tcgctttact gagaaataat gaagctatta
     7081 atattgctcg tttagcttac ttattagcaa gaagcaagat gaatgaggat tttacgtcta
     7141 aaatttttaa ttgggctcaa aacgacaaag ataaaaatca attaattaca gcgttagagt
     7201 attatattta tcaaataagg gaggctgatt gagtatgcat caccatcacc atcacgctag
     7261 catattagct aaaactaaaa gtggtaaaac gatagatttg acttttgcac atgaggtcgt
     7321 aaaaagtaat gtaaaaaatg ttaaagatag aaaaggcaaa gaaaaacaag ttttatttaa
     7381 cgggcttaca acaagcaagt taagaaattt aatggagcag gtaaatagac tttatactat
     7441 tgcatttaat tcgaatgagg atcaattgaa tgaagaattc attgatgaat tagaatattt
     7501 aaaaattaaa ttttattatg aagcaggacg agaaaaaagc gttgatgaat ttttaaaaaa
     7561 aacattgatg tttccaatta ttgatagagt gataaaaaaa gaatcaaaaa aatttttctt
     7621 agattattgt aaatactttg aagctttagt tgcatacgct aaatattatc aaaaggagga
     7681 ttaatatgta ttcaaaaatt aaaatttcag gaacaattga agtagttact ggtttacaca
     7741 tcggcggagg cggtgaatct agtatgattg gagcaattga ttctcctgta gttagagatt
     7801 tgcaaaccaa attacctatc atacctggca gttcaatcaa aggaaaaatg agaaatttat
     7861 tagcaaaaca ttttggcttg aaaatgaaac aagagagtca taaccaagat gatgaacgtg
     7921 tattaagatt atttggctca agtgaaaaag gaaatatcca aagagctcgt ctacaaattt
     7981 ctgatgcatt cttttctgaa aagacaaaag agcattttgc gcaaaatgat attgcctata
     8041 cagaaacgaa atttgagaat acaattaatc gtttaactgc agttgcaaac ccaagacaaa
     8101 ttgaaagagt aacaagagga tctgagtttg actttgtatt tatttacaat gtcgatgaag
     8161 agtcgcaagt tgaggatgat tttgagaata ttgaaaaagc gattcactta ttagagaatg
     8221 actatcttgg tggcggcgga actagaggta acggacgtat tcaatttaaa gatacaaata
     8281 tcgagacagt tgttggagaa tacgatagta caaatcttaa aattaagtag gtgaatgaca
     8341 ttggcaacaa aagtatttaa actttctttt aagactcctg ttcattttgg aaaaaaacgg
     8401 ttgtcagatg gtgaaatgac aatcactgct gatactttgt ttagtgcttt atttattgaa
     8461 acacttcaat tgggtaaaga taccgattgg ttattaaatg atttaatcat tagtgataca
     8521 tttccttatg agaatgagct ttattatctt cctaaacctt tgataaaaat tgactctaaa
     8581 gaagaagata accataaagc atttaaaaag ttaaaatatg ttccggttca tcactataat
     8641 caatatttaa atggagagtt aagcgctgaa gatgcgacag atttaaatga tatttttaat
     8701 attgggtatt tttctctaca aacaaaggtt tcattaatag cacaagaaac tgattcaagt
     8761 gctgatagtg aaccttattc agtgggaaca tttacttttg aacctgaagc gggtttatat
     8821 tttattgcaa aaggatcaga agaaacgctt gaccatttaa ataatattat gactgcatta
     8881 cagtattcag gtttaggtgg taaacgtaat gcaggatacg gacaatttga atatgaaata
     8941 ataaataatc aacaactatc taagttactg aatcaaaatg gaaaacattc tattctttta
     9001 tcaacggcaa tggctaaaaa agaagagata gagagtgctt taaaagaggc gagatacatt
     9061 ttaactaaac gttctggttt cgtacaatca acgaattatt ctgaaatgct agttaaaaaa
     9121 agtgatttct atagcttttc ttcgggttca gtttttaaaa atatctttaa tggtgatatt
     9181 tttaatgttg gccataatgg taaacaccca gtctatcgct atgcaaaacc tttatggttg
     9241 gaggtataag tatgacaata aaaaattatg aagtcgttat taaaacttta ggtccaattc
     9301 atattggtag tggtcaagtt atgaagaagc aagattacat ttatgacttt tataattcta
     9361 aagtttatat gattaatgga aataaactag ttaaattttt aaaaagaaaa aatttacttt
     9421 atacatatca aaactttttg aggtacccac caaaaaatcc aagagaaaat ggacttaaag
     9481 actatttaga cgctcaaaat gttaagcaaa gtgaatggga agcatttgtg agttattctg
     9541 aaaaggtcaa tcaaggtaag aaatatggta acacacgtcc taaaccgcta aatgatttac
     9601 acttaatggt aagagacggt caaaataaag tgtatcttcc aggtagttca atcaaaggtg
     9661 ctatcaaaac aactctcgtg tcaaaatata ataatgaaaa aaacaaagac atttatagca
     9721 aaattaaagt cagcgattca aaacctattg atgaaagtaa tttagcgatt tatcaaaaaa
     9781 tagacattaa taaaagtgaa aaatcaatgc ctttatatag agagtgcata gatgtaaata
     9841 ccgaaataaa atttaagtta acaattgaag atgaaattta ttctattaat gaaattgaac
     9901 aaagtatcca agatttttac aaaaactatt atgataaatg gttagtcgga ttcaaagaaa
     9961 caaaaggtgg aagacgattt gcattagagg gcggtatacc agatgtccta aatcagaata
    10021 ttttgttctt aggtgctggg acaggatttg ttagtaaaac aacacactat caattaaaaa
    10081 atcgaaaaca agctaaacaa gattcttttg agattttaac taaaaaattc cgaggaactt
    10141 atgggaaaat gaaggaaata ccttctaacg taccagttgc tttaaaagga acaactaatc
    10201 aaagtcgtca tacttcatat cagcaaggaa tgtgtaaagt gagttttcaa gagttaaata
    10261 atgaggtgct ataatgaaaa tattatttag tccaataggt aattcagatc catggagaaa
    10321 tgatagagat ggtgcgatgc ttcatatcgt gcgtcattat aatttagata aggttgtatt
    10381 atattttact agaactattt gggaaggaaa tgaaaataga aaaggccata aaatttacga
    10441 atgggaaaaa attatccaaa ctgtttctcc aaatactgaa gtagaaatca ttattgaaaa
    10501 tgtagacaat gctcaagatt atgatgtctt caaggagaaa tttcataagt atttaaaaat
    10561 aattgaagat agctatgaag attgtgaaat aattttgaat gtcactagtg gtacacccca
    10621 aatggaatca acattatgtt tagaatatat cgtctatcct gaaaataaga agtgtgtaca
    10681 agtgagcacg ccaactaagg atagtaatgc aggtatcgaa tattcaaatc ctaaagataa
    10741 agtagaagaa tttgaaatag tcaatgaagt agaaaagaaa tctgaaaaac gttgtaaaga
    10801 aataaacatt ttaagcttta gagaagcaat gattagatct caaattctcg gtttaataga
    10861 taattatgat tatgaaggtg ctcttaattt agtaagtaat caaaaatctt ttcgcaatgg
    10921 gaaattatta agaaaaaaac tactatcatt aacaaaacaa attaaaacac atgaagtttt
    10981 tccagaaatt aatgagaagt acagagatga tgctttaaaa aaatcactat ttcattattt
    11041 actgttaaat atgagatata atcgtcttga tgtagctgaa acgttaatta gagtaaaatc
    11101 tattgctgag tttatactta aaacatacat tgagattcat tggcctactt taataattga
    11161 aaaagatggt aaaccttatc taaatgatga agataattta tcttttgttt ataaatataa
    11221 tctgttatta gaaaaaagaa aacaaaattt tgatgtttca agaattttgg gacttcctgc
    11281 attcattgat atactcacaa ttttagaacc taattctcaa ctattaaaag aagtcaatgc
    11341 agtaaacgat ataaatggtt taagaaattc catagcccat aatttagata cattaaattt
    11401 ggataaaaat aaaaattata agaaaataat gttatctgtt gaagcgataa agaatatgtt
    11461 acacatctca tttcctgaga tagaggaaga agactataat tattttgaag aaaaaaataa
    11521 ggaatttaaa gagctattat gataaataaa attacagtag agttagactt gccagaaagt
    11581 attcggtttc aatatttagg aagtgtttta catggtgtgt taatggatta tctatctgat
    11641 gatattgctg accaattaca tcatgaattt gcttatagcc cattgaaaca aagaatatat
    11701 cataaaaata aaaaaatcat ttgggaaatt gtatgtatgt cagataattt atttaaagag
    11761 gttgttaaac tatttagttc taaaaatagt ttgcttttga aatattatca aacaaatatt
    11821 gacattcaat catttcaaat tgagaagata aatgttcaga acatgatgaa ccaactgtta
    11881 caagtagaag atctaagtcg ttatgtacgt cttaatatac aaacacctat gtcttttaaa
    11941 tatcagaaca gttacatgat ttttcctgat gttaaacgtt tttttagaag tattatgata
    12001 caatttgacg cgttttttga agaatataga atgtacgaca aagaaacatt aaattttcta
    12061 gaaaagaatg ttaatattgt tgactacaaa ttgaaaagta cacgttttaa cttggaaaaa
    12121 gttaaaattc cttcatttac aggagaaata gtatttaaaa ttaaaggacc cttacctttt
    12181 ctacagttaa ctcatttttt attaaagttt ggcgaatttt caggttcagg tataaaaaca
    12241 agcttaggta tgggaaaata tagtataatt taattaagac aaaggaagct gagttggctg
    12301 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
    12361 gttttttgct gaaaggagga actatatccg gat
//
