LOCUS       sequence-22035-e         339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler 
            GR, Copeland TD, Kalkum M, Ge K
  TITLE     PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 
            methyltransferase complex.
  JOURNAL   J Biol Chem. 2007 Jul 13. 282(28):20395-406.
  PUBMED    17500065
REFERENCE   2  (bases 1 to 339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jul 13. 282(28):20395-406."
COMMENT     Sequence Label: pcDNA3 Flag PA1
FEATURES             Location/Qualifiers
     source          1..339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag
       61 ctcggatcct ggccaccatg gactacaagg acgacgatga caagatgtcc cttgctcggg
      121 gccatggaga cactgcggcc agtacggcgg cgcctctgtc tgaagaaggg gaagtgacct
      181 ccggcctcca ggctctggcc gtggaggata ccggaggccc ctctgcctcg gccggtaagg
      241 ccgaggacga gggggaagga ggccgagagg agaccgagcg tgaggggtcc gggggcgagg
      301 aggcgcaggg agaagtcccc agcgctgggg gagaagagc
//
