LOCUS       sequence-22036-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Cho YW, Hong T, Hong S, Guo H, Yu H, Kim D, Guszczynski T, Dressler 
            GR, Copeland TD, Kalkum M, Ge K
  TITLE     PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 
            methyltransferase complex.
  JOURNAL   J Biol Chem. 2007 Jul 13. 282(28):20395-406.
  PUBMED    17500065
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Jul 13. 282(28):20395-406."
COMMENT     Sequence Label: pGEX-5X1 Flag PA1
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..14
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..56
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgatcgaagg tcgtgggatc ctggccacca tggactacaa ggacgacgat gacaagatgt
       61 cccttgctcg gggccatgga gacactgcgg ccagtacggc ggcgcctctg tctgaagaag
      121 gggaagtgac ctccggcctc caggctctgg ccgtggagga taccggaggc ccctctgcct
      181 cggccggtaa ggccgaggac gagggggaag gaggccgaga ggagaccgag cgtgaggggt
      241 ccgggggcga ggaggcgcag ggagaagtcc ccagcgctgg gggagaagag cctgccgagg
      301 aggactccga ggactggtgc gtgccctgca gcgacgagga ggtggagctg cctgcggatg
      361 ggcagccctg gatgcccccg ccctccgaaa tccagcggct ctatgaactg ctggctgccc
      421 acggtactct ggagctgcaa gccgagatcc tgccccgccg gcctcccacg ccggaggccc
      481 agagcgaaga ggagagatcc gatgaggagc cggaggccaa agaagaggaa gaggaaaaac
      541 cacacatgcc cacggaattt gattttgatg atgaaccagt gacaccaaag gactccctga
//
