LOCUS       sequence-220398-        3912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-233
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     174..191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1970..1988)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2056..2160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2382..3173
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3344..3912,1..20)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3833..3852
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
       61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      181 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      241 cgattcatta atcactctgt ggtctcaaat ggcgggtatc aatagagcgg gaccatcggg
      301 cgcttatttt gttggccaca cagaccccga gccagtatcg gggcaagcac acggatccgg
      361 cagcggcgcc agctcctcga acagtccgca ggttcagccg cgaccctcga atactccccc
      421 gtcgaacgcg cccgcaccgc cgccaaccgg acgtgagagg ctttcacgat ccacggcgct
      481 gtcgcgccaa accagggagt ggctggagca gggtatgcct acagcggagg atgccagcgt
      541 gcgtcgtagg ccacaggtga ctgccgatgc cgcaacgccg cgtgcagagg caagacgcac
      601 gccggaggca actgccgatg ccagcgcacc gcgtagaggg gcggttgcac acgccaacag
      661 tatcgttcag caattggtca gtgagggcgc tgatatttcg catactcgta acatgctccg
      721 caatgcaatg aatggcgacg cagtcgcttt ttctcgagta gaacagaaca tatttcgcca
      781 gcatttcccg aacatgccca tgcatggaat cagccgagat tcggaactcg ctatcgagct
      841 ccgtggagcg cttcgtcgag cggttcacca acaggcggcg tcagcgccag tgaggtcgcc
      901 cacgccaaca ccggccagcc ctgcggcatc atcatcgggc agcagtcagc gttctttatt
      961 tggacggttt gcccgtttga tggcgccaaa ccagggacgg tcgtcgaaca ctgccgcctc
     1021 tcagacgccg gtcgacagga gcccgccacg cgtcaaccaa agacccatac gcgtcgacag
     1081 ggctgcgatg cgtaatcgtg gcaatgacga ggcggacgcc gcgctgcggg ggttagtaca
     1141 acagggggtc aatttagagc acctgcgcac ggcccttgaa agacatgtaa tgcagcgcct
     1201 ccctatcccc ctcgatatag gcagcgcgtt gcagaatgtg ggaattaacc caagtatcga
     1261 cttgggggaa agccttgtgc aacatcccct gctgaatttg aatgtagcgt tgaatcgcat
     1321 gctggggctg cgtcccagcg ctgaaagagc gcctcgtcca gccgtccccg tggctcccgc
     1381 gaccgcctcc aggcgaccgg atggtacgcg tgcaacacga ttgcgggtga tgccggagcg
     1441 ggaggattac gaaaataatg tggcctatgg agtgcgcttg cttaacctga acccgggggt
     1501 gggggtaagg caggctgttg cggcctttgt aaccgaccgg gctgagcggc cagcagtggt
     1561 ggctaatatc cgggcagccc tggaccctat cgcgtcacaa ttcagtcagc tgcgcacaat
     1621 ttcgaaggcc gatgctgaat ctgaagagct gggttttaag gatgcggcag atcatcacac
     1681 ggatgacgtg acgcactgtc tttttggcgg agaattgtcg ctgagtaatc cggatcagca
     1741 ggtgatcggt ttggcgggta atccgacgga cacgtcgcag ccttacagcc aagagggaaa
     1801 taaggacctg gcgttcatgg atatgaaaaa acttgcccaa ttcctcgcag gcaagcctga
     1861 gcatccgatg accagagaaa cgcttaacgc cgaaaatatc gccaagtatg cttttagaat
     1921 agtcccctga gctttgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata
     1981 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2101 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2161 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     2221 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat
     2281 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2341 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     2401 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2461 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg
     2521 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2581 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2641 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2701 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2761 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2821 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2881 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2941 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3001 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3061 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3121 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag
     3181 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3241 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3301 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3361 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3421 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3481 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3541 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3601 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3661 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3721 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3781 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3841 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3901 gatgctcgtc ag
//
