LOCUS       sequence-220451-       11622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway vector for generating red fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 11622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Rh
FEATURES             Location/Qualifiers
     source          1..11622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    49..173
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(198..203,204..221,222..228)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             282..941
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(348..367)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1283..1588
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1467..1486
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1629..1753)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1773..2447
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             1773..2447
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     2171..2190
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2365..2388
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      2602..3173
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     primer_bind     complement(3214..3230)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3214..3230)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3227..3249)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3238..3254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3262..3268,3269..3286,3287..3292))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3307..3328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             3808..4833
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    4864..5038
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5116..5140
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5565..6359
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6446..7034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6935..6954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7188..7205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7220..7360
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7286..7305)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7446..7468
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7704..7898
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7964..9037)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9466..10095)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11396..11420
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     join(11608..11622,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(11622,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagcttacga acgatagaca tctggatcac aagtttgtac
       61 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      121 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
      181 cattaggcac cccaggcttt acactttatg cttccggctc gtataacgtg tggattttga
      241 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      301 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      361 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
      421 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
      481 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
      541 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
      601 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
      661 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
      721 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
      781 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
      841 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
      901 aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac
      961 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     1021 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     1081 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     1141 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     1201 gaaaaccagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     1261 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     1321 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     1381 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     1441 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     1501 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     1561 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     1621 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     1681 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     1741 tgtacaaagt ggtgataggg cccgggatcc tgatggcctc ctccgaggac gtcatcaagg
     1801 agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg
     1861 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     1921 agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttccag tacggctcca
     1981 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     2041 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     2101 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     2161 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgga
     2221 tgtaccccga ggacggcgcc ctgaagggcg agatcaagat gaggctgaag ctgaaggacg
     2281 gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc gtgcagctgc
     2341 ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag gactacacca
     2401 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgccaga tccacttaac
     2461 gttactgaaa tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc
     2521 agctccggag ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa
     2581 gcagcaaaga gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt
     2641 tcgggtttac ctcttccaga tacagctcat ctgcaatgca ttaatgcatt ggacctcgca
     2701 accctagtac gcccttcagg ctccggcgaa gcagaagaat agcttagcag agtctatttt
     2761 cattttcggg agacgagatc aagcagatca acggtcgtca agagacctac gagactgagg
     2821 aatccgctct tggctccacg cgactatata tttgtctcta attgtacttt gacatgctcc
     2881 tcttctttac tctgatagct tgactatgaa aattccgtca ccagcccctg ggttcgcaaa
     2941 gataattgca ctgtttcttc cttgaactct caagcctaca ggacacacat tcatcgtagg
     3001 tataaacctc gaaaatcatt cctactaaga tgggtataca atagtaacca tgcatggttg
     3061 cctagtgaat gctccgtaac acccaatacg ccggccgaaa cttttttaca actctcctat
     3121 gagtcgttta cccagaatgc acaggtacac ttgtttagag gtaatccttc tttctagagg
     3181 atccccgggt accgagctcg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     3241 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     3301 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3361 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3421 gcgtattggc tagagcagct tgccaaaact gatattgaag gagcattttt tgggcttggc
     3481 tggagctagt ggaggtcaac aatgaatgcc tattttggtt tagtcgtcca ggcggtgagc
     3541 acaaaatttg tgtcgtttga caagatggtt catttaggca actggtcaga tcagccccac
     3601 ttgtagcagt agcggcggcg ctcgaagtgt gactcttatt agcagacagg aacgaggaca
     3661 ttattatcat ctgctgcttg gtgcacgata acttggtgcg tttgtcaagc aaggtaagtg
     3721 gacgacccgg tcataccttc ttaagttcgc ccttcctccc tttatttcag attcaatctg
     3781 acttacctat tctacccaag catccaaatg aaaaagcctg aactcaccgc gacgtctgtc
     3841 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     3901 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     3961 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     4021 ctcccgattc cggaagtgct tgacattggg gagttcagcg agagcctgac ctattgcatc
     4081 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     4141 ctccagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     4201 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     4261 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     4321 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     4381 cggcacctcg tgcatgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     4441 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     4501 atcctcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     4561 aggcatccgg agcttgcagg atcgccgcgc ctccgggcgt atatgctccg cattggtctt
     4621 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     4681 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     4741 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     4801 cgccccagca ctcgtccgag ggcaaaggaa tagagtagat gccgaccggg aaccagtttc
     4861 gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg
     4921 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     4981 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc
     5041 ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     5101 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     5161 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     5221 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     5281 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     5341 tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc
     5401 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
     5461 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
     5521 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
     5581 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     5641 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     5701 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     5761 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     5821 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     5881 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     5941 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     6001 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
     6061 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     6121 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     6181 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     6241 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     6301 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
     6361 acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7141 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7201 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7261 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7321 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     7381 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     7441 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     7501 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
     7561 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
     7621 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
     7681 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
     7741 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
     7801 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
     7861 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
     7921 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
     7981 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
     8041 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
     8101 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
     8161 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
     8221 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
     8281 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
     8341 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
     8401 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
     8461 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
     8521 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
     8581 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
     8641 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
     8701 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
     8761 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
     8821 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
     8881 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
     8941 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
     9001 cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt
     9061 tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt
     9121 tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc
     9181 gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta
     9241 gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca
     9301 tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg
     9361 tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac
     9421 ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga
     9481 tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc
     9541 aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga
     9601 ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt
     9661 gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat
     9721 gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg
     9781 caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg
     9841 gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat
     9901 cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc
     9961 gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag
    10021 ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac
    10081 agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac
    10141 gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg
    10201 cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa
    10261 ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac
    10321 ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg
    10381 tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt
    10441 cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca
    10501 cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag
    10561 ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg
    10621 ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc
    10681 cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg
    10741 tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca
    10801 ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg
    10861 ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc
    10921 tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc
    10981 agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc
    11041 gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct
    11101 agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg
    11161 gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg
    11221 gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac
    11281 tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac
    11341 cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta
    11401 cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct
    11461 gatccaagct caagctgctc tagcattcgc cattcaggct gcgcaactgt tgggaagggc
    11521 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    11581 gattaagttg ggtaacgcca gggttttccc agtcacgacg tt
//
