LOCUS       sequence-220471-       15747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15747)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             5087..5110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      13836..13845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14540..14569
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14570..14593
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14683..14703)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15101..15112)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15337..15471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15374..15393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15428..15447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15500..15665
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggact acaaagacga
     5101 tgacgacaag atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt
     5161 ccaacagcag caacagcaac aacagcaaca acagcagcaa cagcagccgc caccgccgcc
     5221 gccgccgccg ccgcctcctc agcttcctca gccgccgccg caggcacagc cgctgctgcc
     5281 tcagccgcag ccgcccccgc cgccgccccc gccgccaccc ggcccggctg tggctgagga
     5341 gccgctgcac cgaccaaaga aagaactttc agctaccaag aaagaccgtg tgaatcattg
     5401 tctgacaata tgtgaaaaca tagtggcaca gtctgtcaga aattctccag aatttcagaa
     5461 acttctgggc atcgctatgg aactttttct gctgtgcagt gatgacgcag agtcagatgt
     5521 caggatggtg gctgacgaat gcctcaacaa agttatcaaa gctttgatgg attctaatct
     5581 tccaaggtta cagctcgagc tctataagga aattaaaaag aatggtgccc ctcggagttt
     5641 gcgtgctgcc ctgtggaggt ttgctgagct ggctcacctg gttcggcctc agaaatgcag
     5701 gccttacctg gtgaaccttc tgccgtgcct gactcgaaca agcaagagac ccgaagaatc
     5761 agtccaggag accttggctg cagctgttcc caaaattatg gcttcttttg gcaattttgc
     5821 aaatgacaat gaaattaagg ttttgttaaa ggccttcata gcgaacctga agtcaagctc
     5881 ccccaccatt cggcggacag cggctggatc agcagtgagc atctgccagc actcaagaag
     5941 gacacaatat ttctatagtt ggctactaaa tgtgctctta ggcttactcg ttcctgtcga
     6001 ggatgaacac tccactctgc tgattcttgg cgtgctgctc accctgaggt atttggtgcc
     6061 cttgctgcag cagcaggtca aggacacaag cctgaaaggc agcttcggag tgacaaggaa
     6121 agaaatggaa gtctctcctt ctgcagagca gcttgtccag gtttatgaac tgacgttaca
     6181 tcatacacag caccaagacc acaatgttgt gaccggagcc ctggagctgt tgcagcagct
     6241 cttcagaacg cctccacccg agcttctgca aaccctgacc gcagtcgggg gcattgggca
     6301 gctcaccgct gctaaggagg agtctggtgg ccgaagccgt agtgggagta ttgtggaact
     6361 tatagctgga gggggttcct catgcagccc tgtcctttca agaaaacaaa aaggcaaagt
     6421 gctcttagga gaagaagaag ccttggagga tgactctgaa tcgagatcgg atgtcagcag
     6481 ctctgcctta acagcctcag tgaaggatga gatcagtgga gagctggctg cttcttcagg
     6541 ggtttccact ccagggtcag caggtcatga catcatcaca gaacagccac ggtcacagca
     6601 cacactgcag gcggactcag tggatctggc cagctgtgac ttgacaagct ctgccactga
     6661 tggggatgag gaggatatct tgagccacag ctccagccag gtcagcgccg tcccatctga
     6721 ccctgccatg gacctgaatg atgggaccca ggcctcgtcg cccatcagcg acagctccca
     6781 gaccaccacc gaagggcctg attcagctgt taccccttca gacagttctg aaattgtgtt
     6841 agacggtacc gacaaccagt atttgggcct gcagattgga cagccccagg atgaagatga
     6901 ggaagccaca ggtattcttc ctgatgaagc ctcggaggcc ttcaggaact cttccatggc
     6961 ccttcaacag gcacatttat tgaaaaacat gagtcactgc aggcagcctt ctgacagcag
     7021 tgttgataaa tttgtgttga gagatgaagc tactgaaccg ggtgatcaag aaaacaagcc
     7081 ttgccgcatc aaaggtgaca ttggacagtc cactgatgat gactctgcac ctcttgtcca
     7141 ttgtgtccgc cttttatctg cttcgttttt gctaacaggg ggaaaaaatg tgctggttcc
     7201 ggacagggat gtgagggtca gcgtgaaggc cctggccctc agctgtgtgg gagcagctgt
     7261 ggccctccac ccggaatctt tcttcagcaa actctataaa gttcctcttg acaccacgga
     7321 ataccctgag gaacagtatg tctcagacat cttgaactac atcgatcatg gagacccaca
     7381 ggttcgagga gccactgcca ttctctgtgg gaccctcatc tgctccatcc tcagcaggtc
     7441 ccgcttccac gtgggagatt ggatgggcac cattagaacc ctcacaggaa atacattttc
     7501 tttggcggat tgcattcctt tgctgcggaa aacactgaag gatgagtctt ctgttacttg
     7561 caagttagct tgtacagctg tgaggaactg tgtcatgagt ctctgcagca gcagctacag
     7621 tgagttagga ctgcagctga tcatcgatgt gctgactctg aggaacagtt cctattggct
     7681 ggtgaggaca gagcttctgg aaacccttgc agagattgac ttcaggctgg tgagcttttt
     7741 ggaggcaaaa gcagaaaact tacacagagg ggctcatcat tatacagggc ttttaaaact
     7801 gcaagaacga gtgctcaata atgttgtcat ccatttgctt ggagatgaag accccagggt
     7861 gcgacatgtt gccgcagcat cactaattag gcttgtccca aagctgtttt ataaatgtga
     7921 ccaaggacaa gctgatccag tagtggccgt ggcaagagat caaagcagtg tttacctgaa
     7981 acttctcatg catgagacgc agcctccatc tcatttctcc gtcagcacaa taaccagaat
     8041 atatagaggc tataacctac taccaagcat aacagacgtc actatggaaa ataacctttc
     8101 aagagttatt gcagcagttt ctcatgaact aatcacatca accaccagag cactcacatt
     8161 tggatgctgt gaagctttgt gtcttctttc cactgccttc ccagtttgca tttggagttt
     8221 aggttggcac tgtggagtgc ctccactgag tgcctcagat gagtctagga agagctgtac
     8281 cgttgggatg gccacaatga ttctgaccct gctctcgtca gcttggttcc cattggatct
     8341 ctcagcccat caagatgctt tgattttggc cggaaacttg cttgcagcca gtgctcccaa
     8401 atctctgaga agttcatggg cctctgaaga agaagccaac ccagcagcca ccaagcaaga
     8461 ggaggtctgg ccagccctgg gggaccgggc cctggtgccc atggtggagc agctcttctc
     8521 tcacctgctg aaggtgatta acatttgtgc ccacgtcctg gatgacgtgg ctcctggacc
     8581 cgcaataaag gcagccttgc cttctctaac aaacccccct tctctaagtc ccatccgacg
     8641 aaaggggaag gagaaagaac caggagaaca agcatctgta ccgttgagtc ccaagaaagg
     8701 cagtgaggcc agtgcagctt ctagacaatc tgatacctca ggtcctgtta caacaagtaa
     8761 atcctcatca ctggggagtt tctatcatct tccttcatac ctcaaactgc atgatgtcct
     8821 gaaagctaca cacgctaact acaaggtcac gctggatctt cagaacagca cggaaaagtt
     8881 tggagggttt cttcgctcag ccttggatgt tctttctcag atactagagc tggccacact
     8941 gcaggacatt gggaagtgtg ttgaagagat cctaggatac ctgaaatcct gctttagtcg
     9001 agaaccaatg atggcaactg tttgtgttca acaattgttg aagactctct ttggcacaaa
     9061 cttggcctcc cagtttgatg gcttatcttc caaccccagc aagtcacaag gccgagcaca
     9121 gcgccttggc tcctccagtg tgaggccagg cttgtaccac tactgcttca tggccccgta
     9181 cacccacttc acccaggccc tcgctgacgc cagcctgagg aacatggtgc aggcggagca
     9241 ggagaacgac acctcgggat ggtttgatgt cctccagaaa gtgtctaccc agttgaagac
     9301 aaacctcacg agtgtcacaa agaaccgtgc agataagaat gctattcata atcacattcg
     9361 tttgtttgaa cctcttgtta taaaagcttt aaaacagtac acgactacaa catgtgtgca
     9421 gttacagaag caggttttag atttgctggc gcagctggtt cagttacggg ttaattactg
     9481 tcttctggat tcagatcagg tgtttattgg ctttgtattg aaacagtttg aatacattga
     9541 agtgggccag ttcagggaat cagaggcaat cattccaaac atctttttct tcttggtatt
     9601 actatcttat gaacgctatc attcaaaaca gatcattgga attcctaaaa tcattcagct
     9661 ctgtgatggc atcatggcca gtggaaggaa ggctgtgaca catgccatac cggctctgca
     9721 gcccatagtc cacgacctct ttgtattaag aggaacaaat aaagctgatg caggaaaaga
     9781 gcttgaaacc caaaaagagg tggtggtgtc aatgttactg agactcatcc agtaccatca
     9841 ggtgttggag atgttcattc ttgtcctgca gcagtgccac aaggagaatg aagacaagtg
     9901 gaagcgactg tctcgacaga tagctgacat catcctccca atgttagcca aacagcagat
     9961 gcacattgac tctcatgaag cccttggagt gttaaataca ttatttgaga ttttggcccc
    10021 ttcctccctc cgtccggtag acatgctttt acggagtatg ttcgtcactc caaacacaat
    10081 ggcgtccgtg agcactgttc aactgtggat atcgggaatt ctggccattt tgagggttct
    10141 gatttcccag tcaactgaag atattgttct ttctcgtatt caggagctct ccttctctcc
    10201 gtatttaatc tcctgtacag taattaatag gttaagagat ggggacagta cttcaacgct
    10261 agaagaacac agtgaaggga aacaaataaa gaatttgcca gaagaaacat tttcaaggtt
    10321 tctattacaa ctggttggta ttcttttaga agacattgtt acaaaacagc tgaaggtgga
    10381 aatgagtgag cagcaacata ctttctattg ccaggaacta ggcacactgc taatgtgtct
    10441 gatccacatc ttcaagtctg gaatgttccg gagaatcaca gcagctgcca ctaggctgtt
    10501 ccgcagtgat ggctgtggcg gcagtttcta caccctggac agcttgaact tgcgggctcg
    10561 ttccatgatc accacccacc cggccctggt gctgctctgg tgtcagatac tgctgcttgt
    10621 caaccacacc gactaccgct ggtgggcaga agtgcagcag accccgaaaa gacacagtct
    10681 gtccagcaca aagttactta gtccccagat gtctggagaa gaggaggatt ctgacttggc
    10741 agccaaactt ggaatgtgca atagagaaat agtacgaaga ggggctctca ttctcttctg
    10801 tgattatgtc tgtcagaacc tccatgactc cgagcactta acgtggctca ttgtaaatca
    10861 cattcaagat ctgatcagcc tttcccacga gcctccagta caggacttca tcagtgccgt
    10921 tcatcggaac tctgctgcca gcggcctgtt catccaggca attcagtctc gttgtgaaaa
    10981 cctttcaact ccaaccatgc tgaagaaaac tcttcagtgc ttggagggga tccatctcag
    11041 ccagtcggga gctgtgctca cgctgtatgt ggacaggctt ctgtgcaccc ctttccgtgt
    11101 gctggctcgc atggtcgaca tccttgcttg tcgccgggta gaaatgcttc tggctgcaaa
    11161 tttacagagc agcatggccc agttgccaat ggaagaactc aacagaatcc aggaatacct
    11221 tcagagcagc gggctcgctc agagacacca aaggctctat tccctgctgg acaggtttcg
    11281 tctctccacc atgcaagact cacttagtcc ctctcctcca gtctcttccc acccgctgga
    11341 cggggatggg cacgtgtcac tggaaacagt gagtccggac aaagactggt acgttcatct
    11401 tgtcaaatcc cagtgttgga ccaggtcaga ttctgcactg ctggaaggtg cagagctggt
    11461 gaatcggatt cctgctgaag atatgaatgc cttcatgatg aactcggagt tcaacctaag
    11521 cctgctagct ccatgcttaa gcctagggat gagtgaaatt tctggtggcc agaagagtgc
    11581 cctttttgaa gcagcccgtg aggtgactct ggcccgtgtg agcggcaccg tgcagcagct
    11641 ccctgctgtc catcatgtct tccagcccga gctgcctgca gaaccggcgg cctactggag
    11701 caagttgaat gatctgtttg gggatgctgc actgtatcag tccctgccca ctctggcccg
    11761 ggccctggca cagtacctgg tggtggtctc caaactgccc agtcatttgc accttcctcc
    11821 tgagaaagag aaggacattg tgaaattcgt ggtggcaacc cttgaggccc tgtcctggca
    11881 tttgatccat gagcagatcc cgctgagtct ggatctccag gcagggctgg actgctgctg
    11941 cctggccctg cagctgcctg gcctctggag cgtggtctcc tccacagagt ttgtgaccca
    12001 cgcctgctcc ctcatccact gtgtgcactt catcctggag gccgttgcag tgcagcctgg
    12061 agagcagctt cttagtccag aaagaaggac aaatacccca aaagccatca gcgaggagga
    12121 ggaggaagta gatccaaaca cacagaatcc taagtatatc actgcagcct gtgagatggt
    12181 ggcagaaatg gtggagtctc tgcagtcggt gttggccttg ggtcataaaa ggaatagcgg
    12241 cgtgccggcg tttctcacgc cattgctcag gaacatcatc atcagcctgg cccgcctgcc
    12301 ccttgtcaac agctacacac gtgtgccccc actggtgtgg aagcttggat ggtcacccaa
    12361 accgggaggg gattttggca cagcattccc tgagatcccc gtggagttcc tccaggaaaa
    12421 ggaagtcttt aaggagttca tctaccgcat caacacacta ggctggacca gtcgtactca
    12481 gtttgaagaa acttgggcca ccctccttgg tgtcctggtg acgcagcccc tcgtgatgga
    12541 gcaggaggag agcccaccag aagaagacac agagaggacc cagatcaacg tcctggccgt
    12601 gcaggccatc acctcactgg tgctcagtgc aatgactgtg cctgtggccg gcaacccagc
    12661 tgtaagctgc ttggagcagc agccccggaa caagcctctg aaagctctcg acaccaggtt
    12721 tgggaggaag ctgagcatta tcagagggat tgtggagcaa gagattcaag caatggtttc
    12781 aaagagagag aatattgcca cccatcattt atatcaggca tgggatcctg tcccttctct
    12841 gtctccggct actacaggtg ccctcatcag ccacgagaag ctgctgctgc agatcaaccc
    12901 cgagcgggag ctggggagca tgagctacaa actcggccag gtgtccatac actccgtgtg
    12961 gctagggaac agcatcacac ccctgaggga ggaggaatgg gacgaggaag aggaggagga
    13021 ggccgacgcc cctgcacctt cgtcaccacc cacgtctcca gtcaactcca ggaaacaccg
    13081 ggctggagtt gacatccact cctgttcgca gtttttgctt gagttgtaca gccgctggat
    13141 cctgccgtcc agctcagcca ggaggacccc ggccatcctg atcagtgagg tggtcagatc
    13201 ccttctagtg gtctcagact tgttcaccga gcgcaaccag tttgagctga tgtatgtgac
    13261 gctgacagaa ctgcgaaggg tgcacccttc agaagacgag atcctcgctc agtacctggt
    13321 gcctgccacc tgcaaggcag ctgccgtcct tgggatggac aaggccgtgg cggagcctgt
    13381 cagccgcctg ctggagagca cgctcaggag cagccacctg cccagcaggg ttggagccct
    13441 gcacggcatc ctctatgtgc tggagtgcga cctgctggac gacactgcca agcagctcat
    13501 cccggtcatc agcgactatc tcctctccaa cctgaaaggg atcgcccact gcgtgaacat
    13561 tcacagccag cagcacgtac tggtcatgtg tgccactgcg ttttacctca ttgagaacta
    13621 tcctctggac gtagggccgg aattttcagc atcaataata cagatgtgtg gggtgatgct
    13681 gtctggaagt gaggagtcca ccccctccat catttaccac tgtgccctca gaggcctgga
    13741 gcgcctcctg ctctctgagc agctctcccg cctggatgca gaatcgctgg tcaagctgag
    13801 tgtggacaga gtgaacgtgc acagcccgca ccgggccatg gcggctctgg gcctgatgct
    13861 cacctgcatg tacacaggaa aggagaaagt cagtccgggt agaacttcag accctaatcc
    13921 tgcagccccc gacagcgagt cagtgattgt tgctatggag cgggtatctg ttctttttga
    13981 taggatcagg aaaggctttc cttgtgaagc cagagtggtg gccaggatcc tgccccagtt
    14041 tctagacgac ttcttcccac cccaggacat catgaacaaa gtcatcggag agtttctgtc
    14101 caaccagcag ccataccccc agttcatggc caccgtggtg tataaggtgt ttcagactct
    14161 gcacagcacc gggcagtcgt ccatggtccg ggactgggtc atgctgtccc tctccaactt
    14221 cacgcagagg gccccggtcg ccatggccac gtggagcctc tcctgcttct ttgtcagcgc
    14281 gtccaccagc ccgtgggtcg cggcgatcct cccacatgtc atcagcagga tgggcaagct
    14341 ggagcaggtg gacgtgaacc ttttctgcct ggtcgccaca gacttctaca gacaccagat
    14401 agaggaggag ctcgaccgca gggccttcca gtctgtgctt gaggtggttg cagccccagg
    14461 aagcccatat caccggctgc tgacttgttt acgaaatgtc cacaaggtca ccacctgcta
    14521 aaacctctac ttccaatcgc accatcatca ccatcaccat caccaccatg attacaagga
    14581 tgacgacgat aagtgaggcg gccgccaact tgatatcaag cttatcgata atcaacctct
    14641 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
    14701 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
    14761 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
    14821 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
    14881 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
    14941 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
    15001 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
    15061 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
    15121 ccttccttcc cgcgggcgcc cccctcctca cggcgagcga agcttgtcga gaagtactag
    15181 aggatctcta gagcctgcag tctcgacaag cttgtcgaga agtactagag gatcataatc
    15241 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    15301 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    15361 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    15421 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg
    15481 cttgagccta ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt
    15541 tttaattttc gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc
    15601 cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc
    15661 ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg
    15721 tgacaaaccg tcatcttcgg ctacttt
//
