LOCUS       sequence-220474-       15855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15855)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             5087..5110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      13944..13953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14648..14677
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14678..14701
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14791..14811)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15209..15220)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15445..15579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15482..15501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15536..15555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15608..15773
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggact acaaagacga
     5101 tgacgacaag atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt
     5161 ccaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca
     5221 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcaaca
     5281 acagcagcaa cagcaacaac agcagcaaca gcaaccgcca ccgccgccgc cgccgccgcc
     5341 gcctcctcag cttcctcagc cgccgccgca ggcacagccg ctgctgcctc agccgcagcc
     5401 gcccccgccg ccgcccccgc cgccacccgg cccggctgtg gctgaggagc cgctgcaccg
     5461 accaaagaaa gaactttcag ctaccaagaa agaccgtgtg aatcattgtc tgacaatatg
     5521 tgaaaacata gtggcacagt ctgtcagaaa ttctccagaa tttcagaaac ttctgggcat
     5581 cgctatggaa ctttttctgc tgtgcagtga tgacgcagag tcagatgtca ggatggtggc
     5641 tgacgaatgc ctcaacaaag ttatcaaagc tttgatggat tctaatcttc caaggttaca
     5701 gctcgagctc tataaggaaa ttaaaaagaa tggtgcccct cggagtttgc gtgctgccct
     5761 gtggaggttt gctgagctgg ctcacctggt tcggcctcag aaatgcaggc cttacctggt
     5821 gaaccttctg ccgtgcctga ctcgaacaag caagagaccc gaagaatcag tccaggagac
     5881 cttggctgca gctgttccca aaattatggc ttcttttggc aattttgcaa atgacaatga
     5941 aattaaggtt ttgttaaagg ccttcatagc gaacctgaag tcaagctccc ccaccattcg
     6001 gcggacagcg gctggatcag cagtgagcat ctgccagcac tcaagaagga cacaatattt
     6061 ctatagttgg ctactaaatg tgctcttagg cttactcgtt cctgtcgagg atgaacactc
     6121 cactctgctg attcttggcg tgctgctcac cctgaggtat ttggtgccct tgctgcagca
     6181 gcaggtcaag gacacaagcc tgaaaggcag cttcggagtg acaaggaaag aaatggaagt
     6241 ctctccttct gcagagcagc ttgtccaggt ttatgaactg acgttacatc atacacagca
     6301 ccaagaccac aatgttgtga ccggagccct ggagctgttg cagcagctct tcagaacgcc
     6361 tccacccgag cttctgcaaa ccctgaccgc agtcgggggc attgggcagc tcaccgctgc
     6421 taaggaggag tctggtggcc gaagccgtag tgggagtatt gtggaactta tagctggagg
     6481 gggttcctca tgcagccctg tcctttcaag aaaacaaaaa ggcaaagtgc tcttaggaga
     6541 agaagaagcc ttggaggatg actctgaatc gagatcggat gtcagcagct ctgccttaac
     6601 agcctcagtg aaggatgaga tcagtggaga gctggctgct tcttcagggg tttccactcc
     6661 agggtcagca ggtcatgaca tcatcacaga acagccacgg tcacagcaca cactgcaggc
     6721 ggactcagtg gatctggcca gctgtgactt gacaagctct gccactgatg gggatgagga
     6781 ggatatcttg agccacagct ccagccaggt cagcgccgtc ccatctgacc ctgccatgga
     6841 cctgaatgat gggacccagg cctcgtcgcc catcagcgac agctcccaga ccaccaccga
     6901 agggcctgat tcagctgtta ccccttcaga cagttctgaa attgtgttag acggtaccga
     6961 caaccagtat ttgggcctgc agattggaca gccccaggat gaagatgagg aagccacagg
     7021 tattcttcct gatgaagcct cggaggcctt caggaactct tccatggccc ttcaacaggc
     7081 acatttattg aaaaacatga gtcactgcag gcagccttct gacagcagtg ttgataaatt
     7141 tgtgttgaga gatgaagcta ctgaaccggg tgatcaagaa aacaagcctt gccgcatcaa
     7201 aggtgacatt ggacagtcca ctgatgatga ctctgcacct cttgtccatt gtgtccgcct
     7261 tttatctgct tcgtttttgc taacaggggg aaaaaatgtg ctggttccgg acagggatgt
     7321 gagggtcagc gtgaaggccc tggccctcag ctgtgtggga gcagctgtgg ccctccaccc
     7381 ggaatctttc ttcagcaaac tctataaagt tcctcttgac accacggaat accctgagga
     7441 acagtatgtc tcagacatct tgaactacat cgatcatgga gacccacagg ttcgaggagc
     7501 cactgccatt ctctgtggga ccctcatctg ctccatcctc agcaggtccc gcttccacgt
     7561 gggagattgg atgggcacca ttagaaccct cacaggaaat acattttctt tggcggattg
     7621 cattcctttg ctgcggaaaa cactgaagga tgagtcttct gttacttgca agttagcttg
     7681 tacagctgtg aggaactgtg tcatgagtct ctgcagcagc agctacagtg agttaggact
     7741 gcagctgatc atcgatgtgc tgactctgag gaacagttcc tattggctgg tgaggacaga
     7801 gcttctggaa acccttgcag agattgactt caggctggtg agctttttgg aggcaaaagc
     7861 agaaaactta cacagagggg ctcatcatta tacagggctt ttaaaactgc aagaacgagt
     7921 gctcaataat gttgtcatcc atttgcttgg agatgaagac cccagggtgc gacatgttgc
     7981 cgcagcatca ctaattaggc ttgtcccaaa gctgttttat aaatgtgacc aaggacaagc
     8041 tgatccagta gtggccgtgg caagagatca aagcagtgtt tacctgaaac ttctcatgca
     8101 tgagacgcag cctccatctc atttctccgt cagcacaata accagaatat atagaggcta
     8161 taacctacta ccaagcataa cagacgtcac tatggaaaat aacctttcaa gagttattgc
     8221 agcagtttct catgaactaa tcacatcaac caccagagca ctcacatttg gatgctgtga
     8281 agctttgtgt cttctttcca ctgccttccc agtttgcatt tggagtttag gttggcactg
     8341 tggagtgcct ccactgagtg cctcagatga gtctaggaag agctgtaccg ttgggatggc
     8401 cacaatgatt ctgaccctgc tctcgtcagc ttggttccca ttggatctct cagcccatca
     8461 agatgctttg attttggccg gaaacttgct tgcagccagt gctcccaaat ctctgagaag
     8521 ttcatgggcc tctgaagaag aagccaaccc agcagccacc aagcaagagg aggtctggcc
     8581 agccctgggg gaccgggccc tggtgcccat ggtggagcag ctcttctctc acctgctgaa
     8641 ggtgattaac atttgtgccc acgtcctgga tgacgtggct cctggacccg caataaaggc
     8701 agccttgcct tctctaacaa accccccttc tctaagtccc atccgacgaa aggggaagga
     8761 gaaagaacca ggagaacaag catctgtacc gttgagtccc aagaaaggca gtgaggccag
     8821 tgcagcttct agacaatctg atacctcagg tcctgttaca acaagtaaat cctcatcact
     8881 ggggagtttc tatcatcttc cttcatacct caaactgcat gatgtcctga aagctacaca
     8941 cgctaactac aaggtcacgc tggatcttca gaacagcacg gaaaagtttg gagggtttct
     9001 tcgctcagcc ttggatgttc tttctcagat actagagctg gccacactgc aggacattgg
     9061 gaagtgtgtt gaagagatcc taggatacct gaaatcctgc tttagtcgag aaccaatgat
     9121 ggcaactgtt tgtgttcaac aattgttgaa gactctcttt ggcacaaact tggcctccca
     9181 gtttgatggc ttatcttcca accccagcaa gtcacaaggc cgagcacagc gccttggctc
     9241 ctccagtgtg aggccaggct tgtaccacta ctgcttcatg gccccgtaca cccacttcac
     9301 ccaggccctc gctgacgcca gcctgaggaa catggtgcag gcggagcagg agaacgacac
     9361 ctcgggatgg tttgatgtcc tccagaaagt gtctacccag ttgaagacaa acctcacgag
     9421 tgtcacaaag aaccgtgcag ataagaatgc tattcataat cacattcgtt tgtttgaacc
     9481 tcttgttata aaagctttaa aacagtacac gactacaaca tgtgtgcagt tacagaagca
     9541 ggttttagat ttgctggcgc agctggttca gttacgggtt aattactgtc ttctggattc
     9601 agatcaggtg tttattggct ttgtattgaa acagtttgaa tacattgaag tgggccagtt
     9661 cagggaatca gaggcaatca ttccaaacat ctttttcttc ttggtattac tatcttatga
     9721 acgctatcat tcaaaacaga tcattggaat tcctaaaatc attcagctct gtgatggcat
     9781 catggccagt ggaaggaagg ctgtgacaca tgccataccg gctctgcagc ccatagtcca
     9841 cgacctcttt gtattaagag gaacaaataa agctgatgca ggaaaagagc ttgaaaccca
     9901 aaaagaggtg gtggtgtcaa tgttactgag actcatccag taccatcagg tgttggagat
     9961 gttcattctt gtcctgcagc agtgccacaa ggagaatgaa gacaagtgga agcgactgtc
    10021 tcgacagata gctgacatca tcctcccaat gttagccaaa cagcagatgc acattgactc
    10081 tcatgaagcc cttggagtgt taaatacatt atttgagatt ttggcccctt cctccctccg
    10141 tccggtagac atgcttttac ggagtatgtt cgtcactcca aacacaatgg cgtccgtgag
    10201 cactgttcaa ctgtggatat cgggaattct ggccattttg agggttctga tttcccagtc
    10261 aactgaagat attgttcttt ctcgtattca ggagctctcc ttctctccgt atttaatctc
    10321 ctgtacagta attaataggt taagagatgg ggacagtact tcaacgctag aagaacacag
    10381 tgaagggaaa caaataaaga atttgccaga agaaacattt tcaaggtttc tattacaact
    10441 ggttggtatt cttttagaag acattgttac aaaacagctg aaggtggaaa tgagtgagca
    10501 gcaacatact ttctattgcc aggaactagg cacactgcta atgtgtctga tccacatctt
    10561 caagtctgga atgttccgga gaatcacagc agctgccact aggctgttcc gcagtgatgg
    10621 ctgtggcggc agtttctaca ccctggacag cttgaacttg cgggctcgtt ccatgatcac
    10681 cacccacccg gccctggtgc tgctctggtg tcagatactg ctgcttgtca accacaccga
    10741 ctaccgctgg tgggcagaag tgcagcagac cccgaaaaga cacagtctgt ccagcacaaa
    10801 gttacttagt ccccagatgt ctggagaaga ggaggattct gacttggcag ccaaacttgg
    10861 aatgtgcaat agagaaatag tacgaagagg ggctctcatt ctcttctgtg attatgtctg
    10921 tcagaacctc catgactccg agcacttaac gtggctcatt gtaaatcaca ttcaagatct
    10981 gatcagcctt tcccacgagc ctccagtaca ggacttcatc agtgccgttc atcggaactc
    11041 tgctgccagc ggcctgttca tccaggcaat tcagtctcgt tgtgaaaacc tttcaactcc
    11101 aaccatgctg aagaaaactc ttcagtgctt ggaggggatc catctcagcc agtcgggagc
    11161 tgtgctcacg ctgtatgtgg acaggcttct gtgcacccct ttccgtgtgc tggctcgcat
    11221 ggtcgacatc cttgcttgtc gccgggtaga aatgcttctg gctgcaaatt tacagagcag
    11281 catggcccag ttgccaatgg aagaactcaa cagaatccag gaataccttc agagcagcgg
    11341 gctcgctcag agacaccaaa ggctctattc cctgctggac aggtttcgtc tctccaccat
    11401 gcaagactca cttagtccct ctcctccagt ctcttcccac ccgctggacg gggatgggca
    11461 cgtgtcactg gaaacagtga gtccggacaa agactggtac gttcatcttg tcaaatccca
    11521 gtgttggacc aggtcagatt ctgcactgct ggaaggtgca gagctggtga atcggattcc
    11581 tgctgaagat atgaatgcct tcatgatgaa ctcggagttc aacctaagcc tgctagctcc
    11641 atgcttaagc ctagggatga gtgaaatttc tggtggccag aagagtgccc tttttgaagc
    11701 agcccgtgag gtgactctgg cccgtgtgag cggcaccgtg cagcagctcc ctgctgtcca
    11761 tcatgtcttc cagcccgagc tgcctgcaga accggcggcc tactggagca agttgaatga
    11821 tctgtttggg gatgctgcac tgtatcagtc cctgcccact ctggcccggg ccctggcaca
    11881 gtacctggtg gtggtctcca aactgcccag tcatttgcac cttcctcctg agaaagagaa
    11941 ggacattgtg aaattcgtgg tggcaaccct tgaggccctg tcctggcatt tgatccatga
    12001 gcagatcccg ctgagtctgg atctccaggc agggctggac tgctgctgcc tggccctgca
    12061 gctgcctggc ctctggagcg tggtctcctc cacagagttt gtgacccacg cctgctccct
    12121 catccactgt gtgcacttca tcctggaggc cgttgcagtg cagcctggag agcagcttct
    12181 tagtccagaa agaaggacaa ataccccaaa agccatcagc gaggaggagg aggaagtaga
    12241 tccaaacaca cagaatccta agtatatcac tgcagcctgt gagatggtgg cagaaatggt
    12301 ggagtctctg cagtcggtgt tggccttggg tcataaaagg aatagcggcg tgccggcgtt
    12361 tctcacgcca ttgctcagga acatcatcat cagcctggcc cgcctgcccc ttgtcaacag
    12421 ctacacacgt gtgcccccac tggtgtggaa gcttggatgg tcacccaaac cgggagggga
    12481 ttttggcaca gcattccctg agatccccgt ggagttcctc caggaaaagg aagtctttaa
    12541 ggagttcatc taccgcatca acacactagg ctggaccagt cgtactcagt ttgaagaaac
    12601 ttgggccacc ctccttggtg tcctggtgac gcagcccctc gtgatggagc aggaggagag
    12661 cccaccagaa gaagacacag agaggaccca gatcaacgtc ctggccgtgc aggccatcac
    12721 ctcactggtg ctcagtgcaa tgactgtgcc tgtggccggc aacccagctg taagctgctt
    12781 ggagcagcag ccccggaaca agcctctgaa agctctcgac accaggtttg ggaggaagct
    12841 gagcattatc agagggattg tggagcaaga gattcaagca atggtttcaa agagagagaa
    12901 tattgccacc catcatttat atcaggcatg ggatcctgtc ccttctctgt ctccggctac
    12961 tacaggtgcc ctcatcagcc acgagaagct gctgctgcag atcaaccccg agcgggagct
    13021 ggggagcatg agctacaaac tcggccaggt gtccatacac tccgtgtggc tagggaacag
    13081 catcacaccc ctgagggagg aggaatggga cgaggaagag gaggaggagg ccgacgcccc
    13141 tgcaccttcg tcaccaccca cgtctccagt caactccagg aaacaccggg ctggagttga
    13201 catccactcc tgttcgcagt ttttgcttga gttgtacagc cgctggatcc tgccgtccag
    13261 ctcagccagg aggaccccgg ccatcctgat cagtgaggtg gtcagatccc ttctagtggt
    13321 ctcagacttg ttcaccgagc gcaaccagtt tgagctgatg tatgtgacgc tgacagaact
    13381 gcgaagggtg cacccttcag aagacgagat cctcgctcag tacctggtgc ctgccacctg
    13441 caaggcagct gccgtccttg ggatggacaa ggccgtggcg gagcctgtca gccgcctgct
    13501 ggagagcacg ctcaggagca gccacctgcc cagcagggtt ggagccctgc acggcatcct
    13561 ctatgtgctg gagtgcgacc tgctggacga cactgccaag cagctcatcc cggtcatcag
    13621 cgactatctc ctctccaacc tgaaagggat cgcccactgc gtgaacattc acagccagca
    13681 gcacgtactg gtcatgtgtg ccactgcgtt ttacctcatt gagaactatc ctctggacgt
    13741 agggccggaa ttttcagcat caataataca gatgtgtggg gtgatgctgt ctggaagtga
    13801 ggagtccacc ccctccatca tttaccactg tgccctcaga ggcctggagc gcctcctgct
    13861 ctctgagcag ctctcccgcc tggatgcaga atcgctggtc aagctgagtg tggacagagt
    13921 gaacgtgcac agcccgcacc gggccatggc ggctctgggc ctgatgctca cctgcatgta
    13981 cacaggaaag gagaaagtca gtccgggtag aacttcagac cctaatcctg cagcccccga
    14041 cagcgagtca gtgattgttg ctatggagcg ggtatctgtt ctttttgata ggatcaggaa
    14101 aggctttcct tgtgaagcca gagtggtggc caggatcctg ccccagtttc tagacgactt
    14161 cttcccaccc caggacatca tgaacaaagt catcggagag tttctgtcca accagcagcc
    14221 atacccccag ttcatggcca ccgtggtgta taaggtgttt cagactctgc acagcaccgg
    14281 gcagtcgtcc atggtccggg actgggtcat gctgtccctc tccaacttca cgcagagggc
    14341 cccggtcgcc atggccacgt ggagcctctc ctgcttcttt gtcagcgcgt ccaccagccc
    14401 gtgggtcgcg gcgatcctcc cacatgtcat cagcaggatg ggcaagctgg agcaggtgga
    14461 cgtgaacctt ttctgcctgg tcgccacaga cttctacaga caccagatag aggaggagct
    14521 cgaccgcagg gccttccagt ctgtgcttga ggtggttgca gccccaggaa gcccatatca
    14581 ccggctgctg acttgtttac gaaatgtcca caaggtcacc acctgctaaa acctctactt
    14641 ccaatcgcac catcatcacc atcaccatca ccaccatgat tacaaggatg acgacgataa
    14701 gtgaggcggc cgccaacttg atatcaagct tatcgataat caacctctgg attacaaaat
    14761 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
    14821 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
    14881 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
    14941 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
    15001 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
    15061 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
    15121 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
    15181 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
    15241 cgggcgcccc cctcctcacg gcgagcgaag cttgtcgaga agtactagag gatctctaga
    15301 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag ccataccaca
    15361 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    15421 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    15481 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    15541 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctagg
    15601 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
    15661 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
    15721 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
    15781 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
    15841 atcttcggct acttt
//
