LOCUS       sequence-220480-        8690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite (attR3/attR4) Gateway vector for expression in oomycetes. 
            Confers resistance to geneticin (G418). Contains additional cassette
            for constitutive expression of mTFP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Evangelisti E, Shenhav L, Yunusov T, Le Naour-Vernet M, Rink P, 
            Schornack S
  TITLE     Hydrodynamic Shape Changes Underpin Nuclear Rerouting in Branched 
            Hyphae of an Oomycete Pathogen.
  JOURNAL   MBio. 2019 Oct 1;10(5). pii: mBio.01516-19. doi: 
            10.1128/mBio.01516-19.
  PUBMED    31575765
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-10-1"; volume: "10(5). pii: mBio.01516-19. doi"; pages: 
            " 10.1128/mBio.01516-19"
COMMENT     Sequence Label: pTORKm43GWC
FEATURES             Location/Qualifiers
     source          1..8690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(95..100,101..118,119..125)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             179..859
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(245..264)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1179..1484
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVFTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1363..1382
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1525..1648)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2345..3055)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     primer_bind     complement(3061..3077)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3062..3078)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(3671..3689)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3710..3726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3710..3726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3723..3745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3734..3750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3758..3764,3765..3782,3783..3788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3803..3824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3941..3958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4112..4700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4192..4211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4871..5662,5663..5731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5494..5513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5732..5836)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5863..6318
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5950..5969)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6160..6181
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6444..6466
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6458..6475
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6459..6475
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6482..6501
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6482..6500
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(7229..8023)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7340..7359)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7950..7969
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     protein_bind    join(8637..8690,1..70)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
       61 agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      121 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
      181 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
      241 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
      301 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
      361 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
      421 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
      481 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
      541 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
      601 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
      661 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
      721 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
      781 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcaggcgg ggcgtaatct
      841 agaggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
      901 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
      961 agcagcgtat cacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     1021 tgtcagtatc tccggttcgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     1081 cgaacgctgg aaagcggaaa atcaagaagg gatggctgag gtcgcccggt ttattgaaat
     1141 gaacggctct tttgccgacg agaacagggg ctggtgaaat gcagtttaag gttttcacct
     1201 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     1261 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtccccc
     1321 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     1381 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagt caccgcgaaa
     1441 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
     1501 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
     1561 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     1621 tctcgttcaa cttttctata caaagttgat agcttggcat atgacgggaa tagggacgcg
     1681 ggtggatatt aggagcggca gctgacgtgg cagctcatag acaagggggt gcttgaaagg
     1741 ctgtctatgg tggtgacgcc gtcttgcggt gtggcaatga gttgtatcat ttaatcgaat
     1801 acgaggtata atgggcccgg tacctggttg gtttacgata aataaagaat gattgtcagc
     1861 tggcttaaac atagaaattt ctaccatgtt aagtacacac agttgcctca aaatagtcac
     1921 ggttttagca agaatcgaaa tattgacaaa aagggatcac tccatacgac tatagcggtc
     1981 atcaaccaaa gagaaacaat tctaagtagc gtggggtatg gttttctttg cattagaagt
     2041 cacgtagttt tgtttcaagc cgccatcagt ctactctact ttggtcttta ttctctgtta
     2101 taaaataaag ctgtcactgc gcttgttcag cgttatattt catttgtaaa gcgccatcac
     2161 cgattcgtca cactcgaata ccaaatgcta gtataaaaaa aaattgttct acaaacggcc
     2221 ttcttttgaa aacaatcgtt ctttcacaaa aaaaacaacg aaaatggaaa aaaggaaatt
     2281 gtattaaatg catagacaca aaatctgcaa cttcgcactc agtgcacaca gctcgacctt
     2341 cggcctactt gtacagctcg tccatgccgt cggtggagtt gcgggccacg gcgctctcgt
     2401 aaacggtcac cttgttgtag tccttgtcgt ggttcaggat ctcgatgcgg tggtccacaa
     2461 agtgatagtc gggcagcttc accgccttct tggccctgta gatggtcttg aagtcaacgc
     2521 ggtggtggcc gccgccctcc agcagcagct tgtgcttgac gtcgcccttc agcacgccgt
     2581 cgcgcacgta catcctctcg gtggaggcgt cccagccggt ggtcttcttc tgcatcacgg
     2641 ggccgttggg ggggaagttc tcgcccttga ggtgtatctc gtagatgaag gagtcctcct
     2701 ccatggagat gtcggacttc accttcacga tgcccttgtc ctcgaaggtc atggtgcgct
     2761 cccaagagta gccctcgggg aaggactgct tgaagtagtt ggggatgtcg tcggggtact
     2821 tggtgaaggc cctgttgccg taggcgaacg cggtggtcag aatgtcgtag gagaagggca
     2881 ggggggctcc ctccttcacc tccaggttga tggtgttggt gccgtcgtag ggcttgccct
     2941 cgccctcgcc ctcgatcacg aaggcgtggc cattcacgtt gccctccatc ttcagcttga
     3001 tcttcatgtc gggcttgatt acgcccattg tggtctcctc gcccttgctc accatggtat
     3061 cgataccgtc gacctcgagg gggggcccgt tgtcggtgag agtgaaggtc gaaaaagagt
     3121 cggttgggac ttgggcacgt gagagtgaaa aggagaatga gccttccgat cgtgggcgag
     3181 tcgggcgagg ctttttgagc gtccggttgt ggcttcacaa ccccacaaac aggtggtttt
     3241 cggatgtaaa agaggaagtg aaatggccaa tcagaaacga tccctgtgca agagatgtct
     3301 ttaatggggc gcaaaaaaga cgatcaaaaa gagcggataa gagagttccg gcgagacatg
     3361 tggtggtggt gctataatta acggatggta ggacgcaaaa gcgctctgca cgggtcaggt
     3421 tgagaaggtt ctccaatctt ctctctctct gtgacatgtg gtatttctca gaaaccttcg
     3481 tgtaaacgcg agggaatgga cacggaaatt aaggtctgaa aaagggtact tgactcgagg
     3541 tttgtggtta cgccggctga agacgcctaa cctcgcgtgc gatatgccag ctgtcgacat
     3601 tggaaaagcc gagattgtcg cgggtacgta gaggcgaccc tttgtccatc agaggtacca
     3661 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
     3721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     3901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5401 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5461 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5521 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5581 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5641 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5701 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5761 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5821 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     5881 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     5941 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     6001 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     6061 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     6121 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     6181 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     6241 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     6301 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     6361 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     6421 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     6481 gtaatacgac tcactatagg gcgaattgga gctcgggccc caattccccg gatcgtctca
     6541 tagctgctca agacgtttag cagattgagg atgcagatct tctactaatg aaactactgc
     6601 aaactaccaa caaagaggcg accacaaaat ggacagccta tgccgagatt tgtttaagat
     6661 actcgatttg ccgtccaatt ttcatcaaga cgaaacacag tgcatcgaac tgtgttgtaa
     6721 aaattcattt attcttgcaa ttgtgactct caaagattga ttaaacttga aagctggtga
     6781 aagctttcct tcttttggca tgtttgttac taaggcggcg taagcacaat aggcccagac
     6841 tcatgcaaac cagccatagt gaccttctca ctatgtttcg cttgagattt actagactct
     6901 cgggcatctt cacaaaagat gacaaccgaa tcaacaaatt aagtcaacgc taaatcaaat
     6961 atttagaaaa taaattttat tgtcgaaaaa actctgcttt gacaattttc aaactaacca
     7021 attgctaagt attctagtcg accccgggga tccggggggt gggcgaagaa ctccagcatg
     7081 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     7141 ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg
     7201 tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     7261 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     7321 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     7381 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     7441 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct
     7501 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     7561 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     7621 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     7681 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     7741 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     7801 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac
     7861 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     7921 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     7981 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac ggggcccttg
     8041 tgtttcaatt gataaaagtt gagttagatt tgatgtgtag tccagatgga aaatttcaaa
     8101 gtgagcaaac ttgagactcg agtggcgatt gaaagatacg aggggcgctg ccacccacta
     8161 cgtggggtta cgttcgagac tattccgacg ctcgttttag tagatgggaa ttggttgaaa
     8221 tgaagcgatg aagtgcggtt tcgaccaatc gttagctgga ttggacatga ctgcgttctg
     8281 atatgtcggt ccgaaagttc tcgaagcgat ctacagatag ccaatcacag cgttgtgtgc
     8341 tcaaaacctg agatattggc agccacctgt tgacgtgtcg caaggacaca aattggtatt
     8401 ttagttgtca tcccctattt gcgacgaaaa ccgatttgtt aaccaaatta aataagtcga
     8461 tatacagatt gggttgagcc atgaagaagg taagttacga gtttaataac gataaatttt
     8521 tatttaaacc tttgtcttat tttgccacga actggatttc ttttttaatg gaaataatat
     8581 taaataatag aaataatcga ggacacagtc aattgcattg agcatatgtg aattatcaac
     8641 tatgtataat aaagttgaac gagaaacgta aaatgatata aatatcaata
//
