LOCUS       sequence-220481-       15819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15819)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      13872..13881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14567..14590
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14612..14641
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14642..14665
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14755..14775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15173..15184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15409..15543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15446..15465)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15500..15519
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15572..15737
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca
     5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca
     5221 gcaacaacag cagcaacagc aaccgccacc gccgccgccg ccgccgccgc ctcctcagct
     5281 tcctcagccg ccgccgcagg cacagccgct gctgcctcag ccgcagccgc ccccgccgcc
     5341 gcccccgccg ccacccggcc cggctgtggc tgaggagccg ctgcaccgac caaagaaaga
     5401 actttcagct accaagaaag accgtgtgaa tcattgtctg acaatatgtg aaaacatagt
     5461 ggcacagtct gtcagaaatt ctccagaatt tcagaaactt ctgggcatcg ctatggaact
     5521 ttttctgctg tgcagtgatg acgcagagtc agatgtcagg atggtggctg acgaatgcct
     5581 caacaaagtt atcaaagctt tgatggattc taatcttcca aggttacagc tcgagctcta
     5641 taaggaaatt aaaaagaatg gtgcccctcg gagtttgcgt gctgccctgt ggaggtttgc
     5701 tgagctggct cacctggttc ggcctcagaa atgcaggcct tacctggtga accttctgcc
     5761 gtgcctgact cgaacaagca agagacccga agaatcagtc caggagacct tggctgcagc
     5821 tgttcccaaa attatggctt cttttggcaa ttttgcaaat gacaatgaaa ttaaggtttt
     5881 gttaaaggcc ttcatagcga acctgaagtc aagctccccc accattcggc ggacagcggc
     5941 tggatcagca gtgagcatct gccagcactc aagaaggaca caatatttct atagttggct
     6001 actaaatgtg ctcttaggct tactcgttcc tgtcgaggat gaacactcca ctctgctgat
     6061 tcttggcgtg ctgctcaccc tgaggtattt ggtgcccttg ctgcagcagc aggtcaagga
     6121 cacaagcctg aaaggcagct tcggagtgac aaggaaagaa atggaagtct ctccttctgc
     6181 agagcagctt gtccaggttt atgaactgac gttacatcat acacagcacc aagaccacaa
     6241 tgttgtgacc ggagccctgg agctgttgca gcagctcttc agaacgcctc cacccgagct
     6301 tctgcaaacc ctgaccgcag tcgggggcat tgggcagctc accgctgcta aggaggagtc
     6361 tggtggccga agccgtagtg ggagtattgt ggaacttata gctggagggg gttcctcatg
     6421 cagccctgtc ctttcaagaa aacaaaaagg caaagtgctc ttaggagaag aagaagcctt
     6481 ggaggatgac tctgaatcga gatcggatgt cagcagctct gccttaacag cctcagtgaa
     6541 ggatgagatc agtggagagc tggctgcttc ttcaggggtt tccactccag ggtcagcagg
     6601 tcatgacatc atcacagaac agccacggtc acagcacaca ctgcaggcgg actcagtgga
     6661 tctggccagc tgtgacttga caagctctgc cactgatggg gatgaggagg atatcttgag
     6721 ccacagctcc agccaggtca gcgccgtccc atctgaccct gccatggacc tgaatgatgg
     6781 gacccaggcc tcgtcgccca tcagcgacag ctcccagacc accaccgaag ggcctgattc
     6841 agctgttacc ccttcagaca gttctgaaat tgtgttagac ggtaccgaca accagtattt
     6901 gggcctgcag attggacagc cccaggatga agatgaggaa gccacaggta ttcttcctga
     6961 tgaagcctcg gaggccttca ggaactcttc catggccctt caacaggcac atttattgaa
     7021 aaacatgagt cactgcaggc agccttctga cagcagtgtt gataaatttg tgttgagaga
     7081 tgaagctact gaaccgggtg atcaagaaaa caagccttgc cgcatcaaag gtgacattgg
     7141 acagtccact gatgatgact ctgcacctct tgtccattgt gtccgccttt tatctgcttc
     7201 gtttttgcta acagggggaa aaaatgtgct ggttccggac agggatgtga gggtcagcgt
     7261 gaaggccctg gccctcagct gtgtgggagc agctgtggcc ctccacccgg aatctttctt
     7321 cagcaaactc tataaagttc ctcttgacac cacggaatac cctgaggaac agtatgtctc
     7381 agacatcttg aactacatcg atcatggaga cccacaggtt cgaggagcca ctgccattct
     7441 ctgtgggacc ctcatctgct ccatcctcag caggtcccgc ttccacgtgg gagattggat
     7501 gggcaccatt agaaccctca caggaaatac attttctttg gcggattgca ttcctttgct
     7561 gcggaaaaca ctgaaggatg agtcttctgt tacttgcaag ttagcttgta cagctgtgag
     7621 gaactgtgtc atgagtctct gcagcagcag ctacagtgag ttaggactgc agctgatcat
     7681 cgatgtgctg actctgagga acagttccta ttggctggtg aggacagagc ttctggaaac
     7741 ccttgcagag attgacttca ggctggtgag ctttttggag gcaaaagcag aaaacttaca
     7801 cagaggggct catcattata cagggctttt aaaactgcaa gaacgagtgc tcaataatgt
     7861 tgtcatccat ttgcttggag atgaagaccc cagggtgcga catgttgccg cagcatcact
     7921 aattaggctt gtcccaaagc tgttttataa atgtgaccaa ggacaagctg atccagtagt
     7981 ggccgtggca agagatcaaa gcagtgttta cctgaaactt ctcatgcatg agacgcagcc
     8041 tccatctcat ttctccgtca gcacaataac cagaatatat agaggctata acctactacc
     8101 aagcataaca gacgtcacta tggaaaataa cctttcaaga gttattgcag cagtttctca
     8161 tgaactaatc acatcaacca ccagagcact cacatttgga tgctgtgaag ctttgtgtct
     8221 tctttccact gccttcccag tttgcatttg gagtttaggt tggcactgtg gagtgcctcc
     8281 actgagtgcc tcagatgagt ctaggaagag ctgtaccgtt gggatggcca caatgattct
     8341 gaccctgctc tcgtcagctt ggttcccatt ggatctctca gcccatcaag atgctttgat
     8401 tttggccgga aacttgcttg cagccagtgc tcccaaatct ctgagaagtt catgggcctc
     8461 tgaagaagaa gccaacccag cagccaccaa gcaagaggag gtctggccag ccctggggga
     8521 ccgggccctg gtgcccatgg tggagcagct cttctctcac ctgctgaagg tgattaacat
     8581 ttgtgcccac gtcctggatg acgtggctcc tggacccgca ataaaggcag ccttgccttc
     8641 tctaacaaac cccccttctc taagtcccat ccgacgaaag gggaaggaga aagaaccagg
     8701 agaacaagca tctgtaccgt tgagtcccaa gaaaggcagt gaggccagtg cagcttctag
     8761 acaatctgat acctcaggtc ctgttacaac aagtaaatcc tcatcactgg ggagtttcta
     8821 tcatcttcct tcatacctca aactgcatga tgtcctgaaa gctacacacg ctaactacaa
     8881 ggtcacgctg gatcttcaga acagcacgga aaagtttgga gggtttcttc gctcagcctt
     8941 ggatgttctt tctcagatac tagagctggc cacactgcag gacattggga agtgtgttga
     9001 agagatccta ggatacctga aatcctgctt tagtcgagaa ccaatgatgg caactgtttg
     9061 tgttcaacaa ttgttgaaga ctctctttgg cacaaacttg gcctcccagt ttgatggctt
     9121 atcttccaac cccagcaagt cacaaggccg agcacagcgc cttggctcct ccagtgtgag
     9181 gccaggcttg taccactact gcttcatggc cccgtacacc cacttcaccc aggccctcgc
     9241 tgacgccagc ctgaggaaca tggtgcaggc ggagcaggag aacgacacct cgggatggtt
     9301 tgatgtcctc cagaaagtgt ctacccagtt gaagacaaac ctcacgagtg tcacaaagaa
     9361 ccgtgcagat aagaatgcta ttcataatca cattcgtttg tttgaacctc ttgttataaa
     9421 agctttaaaa cagtacacga ctacaacatg tgtgcagtta cagaagcagg ttttagattt
     9481 gctggcgcag ctggttcagt tacgggttaa ttactgtctt ctggattcag atcaggtgtt
     9541 tattggcttt gtattgaaac agtttgaata cattgaagtg ggccagttca gggaatcaga
     9601 ggcaatcatt ccaaacatct ttttcttctt ggtattacta tcttatgaac gctatcattc
     9661 aaaacagatc attggaattc ctaaaatcat tcagctctgt gatggcatca tggccagtgg
     9721 aaggaaggct gtgacacatg ccataccggc tctgcagccc atagtccacg acctctttgt
     9781 attaagagga acaaataaag ctgatgcagg aaaagagctt gaaacccaaa aagaggtggt
     9841 ggtgtcaatg ttactgagac tcatccagta ccatcaggtg ttggagatgt tcattcttgt
     9901 cctgcagcag tgccacaagg agaatgaaga caagtggaag cgactgtctc gacagatagc
     9961 tgacatcatc ctcccaatgt tagccaaaca gcagatgcac attgactctc atgaagccct
    10021 tggagtgtta aatacattat ttgagatttt ggccccttcc tccctccgtc cggtagacat
    10081 gcttttacgg agtatgttcg tcactccaaa cacaatggcg tccgtgagca ctgttcaact
    10141 gtggatatcg ggaattctgg ccattttgag ggttctgatt tcccagtcaa ctgaagatat
    10201 tgttctttct cgtattcagg agctctcctt ctctccgtat ttaatctcct gtacagtaat
    10261 taataggtta agagatgggg acagtacttc aacgctagaa gaacacagtg aagggaaaca
    10321 aataaagaat ttgccagaag aaacattttc aaggtttcta ttacaactgg ttggtattct
    10381 tttagaagac attgttacaa aacagctgaa ggtggaaatg agtgagcagc aacatacttt
    10441 ctattgccag gaactaggca cactgctaat gtgtctgatc cacatcttca agtctggaat
    10501 gttccggaga atcacagcag ctgccactag gctgttccgc agtgatggct gtggcggcag
    10561 tttctacacc ctggacagct tgaacttgcg ggctcgttcc atgatcacca cccacccggc
    10621 cctggtgctg ctctggtgtc agatactgct gcttgtcaac cacaccgact accgctggtg
    10681 ggcagaagtg cagcagaccc cgaaaagaca cagtctgtcc agcacaaagt tacttagtcc
    10741 ccagatgtct ggagaagagg aggattctga cttggcagcc aaacttggaa tgtgcaatag
    10801 agaaatagta cgaagagggg ctctcattct cttctgtgat tatgtctgtc agaacctcca
    10861 tgactccgag cacttaacgt ggctcattgt aaatcacatt caagatctga tcagcctttc
    10921 ccacgagcct ccagtacagg acttcatcag tgccgttcat cggaactctg ctgccagcgg
    10981 cctgttcatc caggcaattc agtctcgttg tgaaaacctt tcaactccaa ccatgctgaa
    11041 gaaaactctt cagtgcttgg aggggatcca tctcagccag tcgggagctg tgctcacgct
    11101 gtatgtggac aggcttctgt gcaccccttt ccgtgtgctg gctcgcatgg tcgacatcct
    11161 tgcttgtcgc cgggtagaaa tgcttctggc tgcaaattta cagagcagca tggcccagtt
    11221 gccaatggaa gaactcaaca gaatccagga ataccttcag agcagcgggc tcgctcagag
    11281 acaccaaagg ctctattccc tgctggacag gtttcgtctc tccaccatgc aagactcact
    11341 tagtccctct cctccagtct cttcccaccc gctggacggg gatgggcacg tgtcactgga
    11401 aacagtgagt ccggacaaag actggtacgt tcatcttgtc aaatcccagt gttggaccag
    11461 gtcagattct gcactgctgg aaggtgcaga gctggtgaat cggattcctg ctgaagatat
    11521 gaatgccttc atgatgaact cggagttcaa cctaagcctg ctagctccat gcttaagcct
    11581 agggatgagt gaaatttctg gtggccagaa gagtgccctt tttgaagcag cccgtgaggt
    11641 gactctggcc cgtgtgagcg gcaccgtgca gcagctccct gctgtccatc atgtcttcca
    11701 gcccgagctg cctgcagaac cggcggccta ctggagcaag ttgaatgatc tgtttgggga
    11761 tgctgcactg tatcagtccc tgcccactct ggcccgggcc ctggcacagt acctggtggt
    11821 ggtctccaaa ctgcccagtc atttgcacct tcctcctgag aaagagaagg acattgtgaa
    11881 attcgtggtg gcaacccttg aggccctgtc ctggcatttg atccatgagc agatcccgct
    11941 gagtctggat ctccaggcag ggctggactg ctgctgcctg gccctgcagc tgcctggcct
    12001 ctggagcgtg gtctcctcca cagagtttgt gacccacgcc tgctccctca tccactgtgt
    12061 gcacttcatc ctggaggccg ttgcagtgca gcctggagag cagcttctta gtccagaaag
    12121 aaggacaaat accccaaaag ccatcagcga ggaggaggag gaagtagatc caaacacaca
    12181 gaatcctaag tatatcactg cagcctgtga gatggtggca gaaatggtgg agtctctgca
    12241 gtcggtgttg gccttgggtc ataaaaggaa tagcggcgtg ccggcgtttc tcacgccatt
    12301 gctcaggaac atcatcatca gcctggcccg cctgcccctt gtcaacagct acacacgtgt
    12361 gcccccactg gtgtggaagc ttggatggtc acccaaaccg ggaggggatt ttggcacagc
    12421 attccctgag atccccgtgg agttcctcca ggaaaaggaa gtctttaagg agttcatcta
    12481 ccgcatcaac acactaggct ggaccagtcg tactcagttt gaagaaactt gggccaccct
    12541 ccttggtgtc ctggtgacgc agcccctcgt gatggagcag gaggagagcc caccagaaga
    12601 agacacagag aggacccaga tcaacgtcct ggccgtgcag gccatcacct cactggtgct
    12661 cagtgcaatg actgtgcctg tggccggcaa cccagctgta agctgcttgg agcagcagcc
    12721 ccggaacaag cctctgaaag ctctcgacac caggtttggg aggaagctga gcattatcag
    12781 agggattgtg gagcaagaga ttcaagcaat ggtttcaaag agagagaata ttgccaccca
    12841 tcatttatat caggcatggg atcctgtccc ttctctgtct ccggctacta caggtgccct
    12901 catcagccac gagaagctgc tgctgcagat caaccccgag cgggagctgg ggagcatgag
    12961 ctacaaactc ggccaggtgt ccatacactc cgtgtggcta gggaacagca tcacacccct
    13021 gagggaggag gaatgggacg aggaagagga ggaggaggcc gacgcccctg caccttcgtc
    13081 accacccacg tctccagtca actccaggaa acaccgggct ggagttgaca tccactcctg
    13141 ttcgcagttt ttgcttgagt tgtacagccg ctggatcctg ccgtccagct cagccaggag
    13201 gaccccggcc atcctgatca gtgaggtggt cagatccctt ctagtggtct cagacttgtt
    13261 caccgagcgc aaccagtttg agctgatgta tgtgacgctg acagaactgc gaagggtgca
    13321 cccttcagaa gacgagatcc tcgctcagta cctggtgcct gccacctgca aggcagctgc
    13381 cgtccttggg atggacaagg ccgtggcgga gcctgtcagc cgcctgctgg agagcacgct
    13441 caggagcagc cacctgccca gcagggttgg agccctgcac ggcatcctct atgtgctgga
    13501 gtgcgacctg ctggacgaca ctgccaagca gctcatcccg gtcatcagcg actatctcct
    13561 ctccaacctg aaagggatcg cccactgcgt gaacattcac agccagcagc acgtactggt
    13621 catgtgtgcc actgcgtttt acctcattga gaactatcct ctggacgtag ggccggaatt
    13681 ttcagcatca ataatacaga tgtgtggggt gatgctgtct ggaagtgagg agtccacccc
    13741 ctccatcatt taccactgtg ccctcagagg cctggagcgc ctcctgctct ctgagcagct
    13801 ctcccgcctg gatgcagaat cgctggtcaa gctgagtgtg gacagagtga acgtgcacag
    13861 cccgcaccgg gccatggcgg ctctgggcct gatgctcacc tgcatgtaca caggaaagga
    13921 gaaagtcagt ccgggtagaa cttcagaccc taatcctgca gcccccgaca gcgagtcagt
    13981 gattgttgct atggagcggg tatctgttct ttttgatagg atcaggaaag gctttccttg
    14041 tgaagccaga gtggtggcca ggatcctgcc ccagtttcta gacgacttct tcccacccca
    14101 ggacatcatg aacaaagtca tcggagagtt tctgtccaac cagcagccat acccccagtt
    14161 catggccacc gtggtgtata aggtgtttca gactctgcac agcaccgggc agtcgtccat
    14221 ggtccgggac tgggtcatgc tgtccctctc caacttcacg cagagggccc cggtcgccat
    14281 ggccacgtgg agcctctcct gcttctttgt cagcgcgtcc accagcccgt gggtcgcggc
    14341 gatcctccca catgtcatca gcaggatggg caagctggag caggtggacg tgaacctttt
    14401 ctgcctggtc gccacagact tctacagaca ccagatagag gaggagctcg accgcagggc
    14461 cttccagtct gtgcttgagg tggttgcagc cccaggaagc ccatatcacc ggctgctgac
    14521 ttgtttacga aatgtccaca aggtcaccac ctgcggtgga agcggtgact acaaagacga
    14581 tgacgacaag taaaacctct acttccaatc gcaccatcat caccatcacc atcaccacca
    14641 tgattacaag gatgacgacg ataagtgagg cggccgccaa cttgatatca agcttatcga
    14701 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
    14761 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
    14821 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
    14881 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
    14941 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
    15001 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
    15061 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
    15121 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
    15181 caatccagcg gaccttcctt cccgcgggcg cccccctcct cacggcgagc gaagcttgtc
    15241 gagaagtact agaggatctc tagagcctgc agtctcgaca agcttgtcga gaagtactag
    15301 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
    15361 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    15421 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    15481 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    15541 atctgatcac tgcttgagcc taggagatcc gaaccagata agtgaaatct agttccaaac
    15601 tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt
    15661 ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac
    15721 tattttgtcc gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct
    15781 gccaactcca tgtgacaaac cgtcatcttc ggctacttt
//
