LOCUS       sequence-220482-       15801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      13854..13863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14549..14572
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14594..14623
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14624..14647
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14737..14757)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15155..15166)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15391..15525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15428..15447)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15482..15501
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15554..15719
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca
     5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca
     5221 gcaaccgcca ccgccgccgc cgccgccgcc gcctcctcag cttcctcagc cgccgccgca
     5281 ggcacagccg ctgctgcctc agccgcagcc gcccccgccg ccgcccccgc cgccacccgg
     5341 cccggctgtg gctgaggagc cgctgcaccg accaaagaaa gaactttcag ctaccaagaa
     5401 agaccgtgtg aatcattgtc tgacaatatg tgaaaacata gtggcacagt ctgtcagaaa
     5461 ttctccagaa tttcagaaac ttctgggcat cgctatggaa ctttttctgc tgtgcagtga
     5521 tgacgcagag tcagatgtca ggatggtggc tgacgaatgc ctcaacaaag ttatcaaagc
     5581 tttgatggat tctaatcttc caaggttaca gctcgagctc tataaggaaa ttaaaaagaa
     5641 tggtgcccct cggagtttgc gtgctgccct gtggaggttt gctgagctgg ctcacctggt
     5701 tcggcctcag aaatgcaggc cttacctggt gaaccttctg ccgtgcctga ctcgaacaag
     5761 caagagaccc gaagaatcag tccaggagac cttggctgca gctgttccca aaattatggc
     5821 ttcttttggc aattttgcaa atgacaatga aattaaggtt ttgttaaagg ccttcatagc
     5881 gaacctgaag tcaagctccc ccaccattcg gcggacagcg gctggatcag cagtgagcat
     5941 ctgccagcac tcaagaagga cacaatattt ctatagttgg ctactaaatg tgctcttagg
     6001 cttactcgtt cctgtcgagg atgaacactc cactctgctg attcttggcg tgctgctcac
     6061 cctgaggtat ttggtgccct tgctgcagca gcaggtcaag gacacaagcc tgaaaggcag
     6121 cttcggagtg acaaggaaag aaatggaagt ctctccttct gcagagcagc ttgtccaggt
     6181 ttatgaactg acgttacatc atacacagca ccaagaccac aatgttgtga ccggagccct
     6241 ggagctgttg cagcagctct tcagaacgcc tccacccgag cttctgcaaa ccctgaccgc
     6301 agtcgggggc attgggcagc tcaccgctgc taaggaggag tctggtggcc gaagccgtag
     6361 tgggagtatt gtggaactta tagctggagg gggttcctca tgcagccctg tcctttcaag
     6421 aaaacaaaaa ggcaaagtgc tcttaggaga agaagaagcc ttggaggatg actctgaatc
     6481 gagatcggat gtcagcagct ctgccttaac agcctcagtg aaggatgaga tcagtggaga
     6541 gctggctgct tcttcagggg tttccactcc agggtcagca ggtcatgaca tcatcacaga
     6601 acagccacgg tcacagcaca cactgcaggc ggactcagtg gatctggcca gctgtgactt
     6661 gacaagctct gccactgatg gggatgagga ggatatcttg agccacagct ccagccaggt
     6721 cagcgccgtc ccatctgacc ctgccatgga cctgaatgat gggacccagg cctcgtcgcc
     6781 catcagcgac agctcccaga ccaccaccga agggcctgat tcagctgtta ccccttcaga
     6841 cagttctgaa attgtgttag acggtaccga caaccagtat ttgggcctgc agattggaca
     6901 gccccaggat gaagatgagg aagccacagg tattcttcct gatgaagcct cggaggcctt
     6961 caggaactct tccatggccc ttcaacaggc acatttattg aaaaacatga gtcactgcag
     7021 gcagccttct gacagcagtg ttgataaatt tgtgttgaga gatgaagcta ctgaaccggg
     7081 tgatcaagaa aacaagcctt gccgcatcaa aggtgacatt ggacagtcca ctgatgatga
     7141 ctctgcacct cttgtccatt gtgtccgcct tttatctgct tcgtttttgc taacaggggg
     7201 aaaaaatgtg ctggttccgg acagggatgt gagggtcagc gtgaaggccc tggccctcag
     7261 ctgtgtggga gcagctgtgg ccctccaccc ggaatctttc ttcagcaaac tctataaagt
     7321 tcctcttgac accacggaat accctgagga acagtatgtc tcagacatct tgaactacat
     7381 cgatcatgga gacccacagg ttcgaggagc cactgccatt ctctgtggga ccctcatctg
     7441 ctccatcctc agcaggtccc gcttccacgt gggagattgg atgggcacca ttagaaccct
     7501 cacaggaaat acattttctt tggcggattg cattcctttg ctgcggaaaa cactgaagga
     7561 tgagtcttct gttacttgca agttagcttg tacagctgtg aggaactgtg tcatgagtct
     7621 ctgcagcagc agctacagtg agttaggact gcagctgatc atcgatgtgc tgactctgag
     7681 gaacagttcc tattggctgg tgaggacaga gcttctggaa acccttgcag agattgactt
     7741 caggctggtg agctttttgg aggcaaaagc agaaaactta cacagagggg ctcatcatta
     7801 tacagggctt ttaaaactgc aagaacgagt gctcaataat gttgtcatcc atttgcttgg
     7861 agatgaagac cccagggtgc gacatgttgc cgcagcatca ctaattaggc ttgtcccaaa
     7921 gctgttttat aaatgtgacc aaggacaagc tgatccagta gtggccgtgg caagagatca
     7981 aagcagtgtt tacctgaaac ttctcatgca tgagacgcag cctccatctc atttctccgt
     8041 cagcacaata accagaatat atagaggcta taacctacta ccaagcataa cagacgtcac
     8101 tatggaaaat aacctttcaa gagttattgc agcagtttct catgaactaa tcacatcaac
     8161 caccagagca ctcacatttg gatgctgtga agctttgtgt cttctttcca ctgccttccc
     8221 agtttgcatt tggagtttag gttggcactg tggagtgcct ccactgagtg cctcagatga
     8281 gtctaggaag agctgtaccg ttgggatggc cacaatgatt ctgaccctgc tctcgtcagc
     8341 ttggttccca ttggatctct cagcccatca agatgctttg attttggccg gaaacttgct
     8401 tgcagccagt gctcccaaat ctctgagaag ttcatgggcc tctgaagaag aagccaaccc
     8461 agcagccacc aagcaagagg aggtctggcc agccctgggg gaccgggccc tggtgcccat
     8521 ggtggagcag ctcttctctc acctgctgaa ggtgattaac atttgtgccc acgtcctgga
     8581 tgacgtggct cctggacccg caataaaggc agccttgcct tctctaacaa accccccttc
     8641 tctaagtccc atccgacgaa aggggaagga gaaagaacca ggagaacaag catctgtacc
     8701 gttgagtccc aagaaaggca gtgaggccag tgcagcttct agacaatctg atacctcagg
     8761 tcctgttaca acaagtaaat cctcatcact ggggagtttc tatcatcttc cttcatacct
     8821 caaactgcat gatgtcctga aagctacaca cgctaactac aaggtcacgc tggatcttca
     8881 gaacagcacg gaaaagtttg gagggtttct tcgctcagcc ttggatgttc tttctcagat
     8941 actagagctg gccacactgc aggacattgg gaagtgtgtt gaagagatcc taggatacct
     9001 gaaatcctgc tttagtcgag aaccaatgat ggcaactgtt tgtgttcaac aattgttgaa
     9061 gactctcttt ggcacaaact tggcctccca gtttgatggc ttatcttcca accccagcaa
     9121 gtcacaaggc cgagcacagc gccttggctc ctccagtgtg aggccaggct tgtaccacta
     9181 ctgcttcatg gccccgtaca cccacttcac ccaggccctc gctgacgcca gcctgaggaa
     9241 catggtgcag gcggagcagg agaacgacac ctcgggatgg tttgatgtcc tccagaaagt
     9301 gtctacccag ttgaagacaa acctcacgag tgtcacaaag aaccgtgcag ataagaatgc
     9361 tattcataat cacattcgtt tgtttgaacc tcttgttata aaagctttaa aacagtacac
     9421 gactacaaca tgtgtgcagt tacagaagca ggttttagat ttgctggcgc agctggttca
     9481 gttacgggtt aattactgtc ttctggattc agatcaggtg tttattggct ttgtattgaa
     9541 acagtttgaa tacattgaag tgggccagtt cagggaatca gaggcaatca ttccaaacat
     9601 ctttttcttc ttggtattac tatcttatga acgctatcat tcaaaacaga tcattggaat
     9661 tcctaaaatc attcagctct gtgatggcat catggccagt ggaaggaagg ctgtgacaca
     9721 tgccataccg gctctgcagc ccatagtcca cgacctcttt gtattaagag gaacaaataa
     9781 agctgatgca ggaaaagagc ttgaaaccca aaaagaggtg gtggtgtcaa tgttactgag
     9841 actcatccag taccatcagg tgttggagat gttcattctt gtcctgcagc agtgccacaa
     9901 ggagaatgaa gacaagtgga agcgactgtc tcgacagata gctgacatca tcctcccaat
     9961 gttagccaaa cagcagatgc acattgactc tcatgaagcc cttggagtgt taaatacatt
    10021 atttgagatt ttggcccctt cctccctccg tccggtagac atgcttttac ggagtatgtt
    10081 cgtcactcca aacacaatgg cgtccgtgag cactgttcaa ctgtggatat cgggaattct
    10141 ggccattttg agggttctga tttcccagtc aactgaagat attgttcttt ctcgtattca
    10201 ggagctctcc ttctctccgt atttaatctc ctgtacagta attaataggt taagagatgg
    10261 ggacagtact tcaacgctag aagaacacag tgaagggaaa caaataaaga atttgccaga
    10321 agaaacattt tcaaggtttc tattacaact ggttggtatt cttttagaag acattgttac
    10381 aaaacagctg aaggtggaaa tgagtgagca gcaacatact ttctattgcc aggaactagg
    10441 cacactgcta atgtgtctga tccacatctt caagtctgga atgttccgga gaatcacagc
    10501 agctgccact aggctgttcc gcagtgatgg ctgtggcggc agtttctaca ccctggacag
    10561 cttgaacttg cgggctcgtt ccatgatcac cacccacccg gccctggtgc tgctctggtg
    10621 tcagatactg ctgcttgtca accacaccga ctaccgctgg tgggcagaag tgcagcagac
    10681 cccgaaaaga cacagtctgt ccagcacaaa gttacttagt ccccagatgt ctggagaaga
    10741 ggaggattct gacttggcag ccaaacttgg aatgtgcaat agagaaatag tacgaagagg
    10801 ggctctcatt ctcttctgtg attatgtctg tcagaacctc catgactccg agcacttaac
    10861 gtggctcatt gtaaatcaca ttcaagatct gatcagcctt tcccacgagc ctccagtaca
    10921 ggacttcatc agtgccgttc atcggaactc tgctgccagc ggcctgttca tccaggcaat
    10981 tcagtctcgt tgtgaaaacc tttcaactcc aaccatgctg aagaaaactc ttcagtgctt
    11041 ggaggggatc catctcagcc agtcgggagc tgtgctcacg ctgtatgtgg acaggcttct
    11101 gtgcacccct ttccgtgtgc tggctcgcat ggtcgacatc cttgcttgtc gccgggtaga
    11161 aatgcttctg gctgcaaatt tacagagcag catggcccag ttgccaatgg aagaactcaa
    11221 cagaatccag gaataccttc agagcagcgg gctcgctcag agacaccaaa ggctctattc
    11281 cctgctggac aggtttcgtc tctccaccat gcaagactca cttagtccct ctcctccagt
    11341 ctcttcccac ccgctggacg gggatgggca cgtgtcactg gaaacagtga gtccggacaa
    11401 agactggtac gttcatcttg tcaaatccca gtgttggacc aggtcagatt ctgcactgct
    11461 ggaaggtgca gagctggtga atcggattcc tgctgaagat atgaatgcct tcatgatgaa
    11521 ctcggagttc aacctaagcc tgctagctcc atgcttaagc ctagggatga gtgaaatttc
    11581 tggtggccag aagagtgccc tttttgaagc agcccgtgag gtgactctgg cccgtgtgag
    11641 cggcaccgtg cagcagctcc ctgctgtcca tcatgtcttc cagcccgagc tgcctgcaga
    11701 accggcggcc tactggagca agttgaatga tctgtttggg gatgctgcac tgtatcagtc
    11761 cctgcccact ctggcccggg ccctggcaca gtacctggtg gtggtctcca aactgcccag
    11821 tcatttgcac cttcctcctg agaaagagaa ggacattgtg aaattcgtgg tggcaaccct
    11881 tgaggccctg tcctggcatt tgatccatga gcagatcccg ctgagtctgg atctccaggc
    11941 agggctggac tgctgctgcc tggccctgca gctgcctggc ctctggagcg tggtctcctc
    12001 cacagagttt gtgacccacg cctgctccct catccactgt gtgcacttca tcctggaggc
    12061 cgttgcagtg cagcctggag agcagcttct tagtccagaa agaaggacaa ataccccaaa
    12121 agccatcagc gaggaggagg aggaagtaga tccaaacaca cagaatccta agtatatcac
    12181 tgcagcctgt gagatggtgg cagaaatggt ggagtctctg cagtcggtgt tggccttggg
    12241 tcataaaagg aatagcggcg tgccggcgtt tctcacgcca ttgctcagga acatcatcat
    12301 cagcctggcc cgcctgcccc ttgtcaacag ctacacacgt gtgcccccac tggtgtggaa
    12361 gcttggatgg tcacccaaac cgggagggga ttttggcaca gcattccctg agatccccgt
    12421 ggagttcctc caggaaaagg aagtctttaa ggagttcatc taccgcatca acacactagg
    12481 ctggaccagt cgtactcagt ttgaagaaac ttgggccacc ctccttggtg tcctggtgac
    12541 gcagcccctc gtgatggagc aggaggagag cccaccagaa gaagacacag agaggaccca
    12601 gatcaacgtc ctggccgtgc aggccatcac ctcactggtg ctcagtgcaa tgactgtgcc
    12661 tgtggccggc aacccagctg taagctgctt ggagcagcag ccccggaaca agcctctgaa
    12721 agctctcgac accaggtttg ggaggaagct gagcattatc agagggattg tggagcaaga
    12781 gattcaagca atggtttcaa agagagagaa tattgccacc catcatttat atcaggcatg
    12841 ggatcctgtc ccttctctgt ctccggctac tacaggtgcc ctcatcagcc acgagaagct
    12901 gctgctgcag atcaaccccg agcgggagct ggggagcatg agctacaaac tcggccaggt
    12961 gtccatacac tccgtgtggc tagggaacag catcacaccc ctgagggagg aggaatggga
    13021 cgaggaagag gaggaggagg ccgacgcccc tgcaccttcg tcaccaccca cgtctccagt
    13081 caactccagg aaacaccggg ctggagttga catccactcc tgttcgcagt ttttgcttga
    13141 gttgtacagc cgctggatcc tgccgtccag ctcagccagg aggaccccgg ccatcctgat
    13201 cagtgaggtg gtcagatccc ttctagtggt ctcagacttg ttcaccgagc gcaaccagtt
    13261 tgagctgatg tatgtgacgc tgacagaact gcgaagggtg cacccttcag aagacgagat
    13321 cctcgctcag tacctggtgc ctgccacctg caaggcagct gccgtccttg ggatggacaa
    13381 ggccgtggcg gagcctgtca gccgcctgct ggagagcacg ctcaggagca gccacctgcc
    13441 cagcagggtt ggagccctgc acggcatcct ctatgtgctg gagtgcgacc tgctggacga
    13501 cactgccaag cagctcatcc cggtcatcag cgactatctc ctctccaacc tgaaagggat
    13561 cgcccactgc gtgaacattc acagccagca gcacgtactg gtcatgtgtg ccactgcgtt
    13621 ttacctcatt gagaactatc ctctggacgt agggccggaa ttttcagcat caataataca
    13681 gatgtgtggg gtgatgctgt ctggaagtga ggagtccacc ccctccatca tttaccactg
    13741 tgccctcaga ggcctggagc gcctcctgct ctctgagcag ctctcccgcc tggatgcaga
    13801 atcgctggtc aagctgagtg tggacagagt gaacgtgcac agcccgcacc gggccatggc
    13861 ggctctgggc ctgatgctca cctgcatgta cacaggaaag gagaaagtca gtccgggtag
    13921 aacttcagac cctaatcctg cagcccccga cagcgagtca gtgattgttg ctatggagcg
    13981 ggtatctgtt ctttttgata ggatcaggaa aggctttcct tgtgaagcca gagtggtggc
    14041 caggatcctg ccccagtttc tagacgactt cttcccaccc caggacatca tgaacaaagt
    14101 catcggagag tttctgtcca accagcagcc atacccccag ttcatggcca ccgtggtgta
    14161 taaggtgttt cagactctgc acagcaccgg gcagtcgtcc atggtccggg actgggtcat
    14221 gctgtccctc tccaacttca cgcagagggc cccggtcgcc atggccacgt ggagcctctc
    14281 ctgcttcttt gtcagcgcgt ccaccagccc gtgggtcgcg gcgatcctcc cacatgtcat
    14341 cagcaggatg ggcaagctgg agcaggtgga cgtgaacctt ttctgcctgg tcgccacaga
    14401 cttctacaga caccagatag aggaggagct cgaccgcagg gccttccagt ctgtgcttga
    14461 ggtggttgca gccccaggaa gcccatatca ccggctgctg acttgtttac gaaatgtcca
    14521 caaggtcacc acctgcggtg gaagcggtga ctacaaagac gatgacgaca agtaaaacct
    14581 ctacttccaa tcgcaccatc atcaccatca ccatcaccac catgattaca aggatgacga
    14641 cgataagtga ggcggccgcc aacttgatat caagcttatc gataatcaac ctctggatta
    14701 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    14761 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    14821 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    14881 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    14941 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    15001 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    15061 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
    15121 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    15181 ttcccgcggg cgcccccctc ctcacggcga gcgaagcttg tcgagaagta ctagaggatc
    15241 tctagagcct gcagtctcga caagcttgtc gagaagtact agaggatcat aatcagccat
    15301 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
    15361 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
    15421 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    15481 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag
    15541 cctaggagat ccgaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
    15601 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
    15661 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag
    15721 cggggcattt ttcttcctgt tatgttttta atcaaacatc ctgccaactc catgtgacaa
    15781 accgtcatct tcggctactt t
//
