LOCUS       sequence-220486-       15867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15867)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      13920..13929
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14615..14638
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14660..14689
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14690..14713
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14803..14823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15221..15232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15457..15591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15494..15513)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15548..15567
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15620..15785
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggagtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca
     5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca
     5221 gcaacaacag caacaacagc agcaacagca acaacagcag caacagcagc aacagcagca
     5281 acagcaacaa ccgccaccgc cgccgccgcc gccgccgcct cctcagcttc ctcagccgcc
     5341 gccgcaggca cagccgctgc tgcctcagcc gcagccgccc ccgccgccgc ccccgccgcc
     5401 acccggcccg gctgtggctg aggagccgct gcaccgacca aagaaagaac tttcagctac
     5461 caagaaagac cgtgtgaatc attgtctgac aatatgtgaa aacatagtgg cacagtctgt
     5521 cagaaattct ccagaatttc agaaacttct gggcatcgct atggaacttt ttctgctgtg
     5581 cagtgatgac gcagagtcag atgtcaggat ggtggctgac gaatgcctca acaaagttat
     5641 caaagctttg atggattcta atcttccaag gttacagctc gagctctata aggaaattaa
     5701 aaagaatggt gcccctcgga gtttgcgtgc tgccctgtgg aggtttgctg agctggctca
     5761 cctggttcgg cctcagaaat gcaggcctta cctggtgaac cttctgccgt gcctgactcg
     5821 aacaagcaag agacccgaag aatcagtcca ggagaccttg gctgcagctg ttcccaaaat
     5881 tatggcttct tttggcaatt ttgcaaatga caatgaaatt aaggttttgt taaaggcctt
     5941 catagcgaac ctgaagtcaa gctcccccac cattcggcgg acagcggctg gatcagcagt
     6001 gagcatctgc cagcactcaa gaaggacaca atatttctat agttggctac taaatgtgct
     6061 cttaggctta ctcgttcctg tcgaggatga acactccact ctgctgattc ttggcgtgct
     6121 gctcaccctg aggtatttgg tgcccttgct gcagcagcag gtcaaggaca caagcctgaa
     6181 aggcagcttc ggagtgacaa ggaaagaaat ggaagtctct ccttctgcag agcagcttgt
     6241 ccaggtttat gaactgacgt tacatcatac acagcaccaa gaccacaatg ttgtgaccgg
     6301 agccctggag ctgttgcagc agctcttcag aacgcctcca cccgagcttc tgcaaaccct
     6361 gaccgcagtc gggggcattg ggcagctcac cgctgctaag gaggagtctg gtggccgaag
     6421 ccgtagtggg agtattgtgg aacttatagc tggagggggt tcctcatgca gccctgtcct
     6481 ttcaagaaaa caaaaaggca aagtgctctt aggagaagaa gaagccttgg aggatgactc
     6541 tgaatcgaga tcggatgtca gcagctctgc cttaacagcc tcagtgaagg atgagatcag
     6601 tggagagctg gctgcttctt caggggtttc cactccaggg tcagcaggtc atgacatcat
     6661 cacagaacag ccacggtcac agcacacact gcaggcggac tcagtggatc tggccagctg
     6721 tgacttgaca agctctgcca ctgatgggga tgaggaggat atcttgagcc acagctccag
     6781 ccaggtcagc gccgtcccat ctgaccctgc catggacctg aatgatggga cccaggcctc
     6841 gtcgcccatc agcgacagct cccagaccac caccgaaggg cctgattcag ctgttacccc
     6901 ttcagacagt tctgaaattg tgttagacgg taccgacaac cagtatttgg gcctgcagat
     6961 tggacagccc caggatgaag atgaggaagc cacaggtatt cttcctgatg aagcctcgga
     7021 ggccttcagg aactcttcca tggcccttca acaggcacat ttattgaaaa acatgagtca
     7081 ctgcaggcag ccttctgaca gcagtgttga taaatttgtg ttgagagatg aagctactga
     7141 accgggtgat caagaaaaca agccttgccg catcaaaggt gacattggac agtccactga
     7201 tgatgactct gcacctcttg tccattgtgt ccgcctttta tctgcttcgt ttttgctaac
     7261 agggggaaaa aatgtgctgg ttccggacag ggatgtgagg gtcagcgtga aggccctggc
     7321 cctcagctgt gtgggagcag ctgtggccct ccacccggaa tctttcttca gcaaactcta
     7381 taaagttcct cttgacacca cggaataccc tgaggaacag tatgtctcag acatcttgaa
     7441 ctacatcgat catggagacc cacaggttcg aggagccact gccattctct gtgggaccct
     7501 catctgctcc atcctcagca ggtcccgctt ccacgtggga gattggatgg gcaccattag
     7561 aaccctcaca ggaaatacat tttctttggc ggattgcatt cctttgctgc ggaaaacact
     7621 gaaggatgag tcttctgtta cttgcaagtt agcttgtaca gctgtgagga actgtgtcat
     7681 gagtctctgc agcagcagct acagtgagtt aggactgcag ctgatcatcg atgtgctgac
     7741 tctgaggaac agttcctatt ggctggtgag gacagagctt ctggaaaccc ttgcagagat
     7801 tgacttcagg ctggtgagct ttttggaggc aaaagcagaa aacttacaca gaggggctca
     7861 tcattataca gggcttttaa aactgcaaga acgagtgctc aataatgttg tcatccattt
     7921 gcttggagat gaagacccca gggtgcgaca tgttgccgca gcatcactaa ttaggcttgt
     7981 cccaaagctg ttttataaat gtgaccaagg acaagctgat ccagtagtgg ccgtggcaag
     8041 agatcaaagc agtgtttacc tgaaacttct catgcatgag acgcagcctc catctcattt
     8101 ctccgtcagc acaataacca gaatatatag aggctataac ctactaccaa gcataacaga
     8161 cgtcactatg gaaaataacc tttcaagagt tattgcagca gtttctcatg aactaatcac
     8221 atcaaccacc agagcactca catttggatg ctgtgaagct ttgtgtcttc tttccactgc
     8281 cttcccagtt tgcatttgga gtttaggttg gcactgtgga gtgcctccac tgagtgcctc
     8341 agatgagtct aggaagagct gtaccgttgg gatggccaca atgattctga ccctgctctc
     8401 gtcagcttgg ttcccattgg atctctcagc ccatcaagat gctttgattt tggccggaaa
     8461 cttgcttgca gccagtgctc ccaaatctct gagaagttca tgggcctctg aagaagaagc
     8521 caacccagca gccaccaagc aagaggaggt ctggccagcc ctgggggacc gggccctggt
     8581 gcccatggtg gagcagctct tctctcacct gctgaaggtg attaacattt gtgcccacgt
     8641 cctggatgac gtggctcctg gacccgcaat aaaggcagcc ttgccttctc taacaaaccc
     8701 cccttctcta agtcccatcc gacgaaaggg gaaggagaaa gaaccaggag aacaagcatc
     8761 tgtaccgttg agtcccaaga aaggcagtga ggccagtgca gcttctagac aatctgatac
     8821 ctcaggtcct gttacaacaa gtaaatcctc atcactgggg agtttctatc atcttccttc
     8881 atacctcaaa ctgcatgatg tcctgaaagc tacacacgct aactacaagg tcacgctgga
     8941 tcttcagaac agcacggaaa agtttggagg gtttcttcgc tcagccttgg atgttctttc
     9001 tcagatacta gagctggcca cactgcagga cattgggaag tgtgttgaag agatcctagg
     9061 atacctgaaa tcctgcttta gtcgagaacc aatgatggca actgtttgtg ttcaacaatt
     9121 gttgaagact ctctttggca caaacttggc ctcccagttt gatggcttat cttccaaccc
     9181 cagcaagtca caaggccgag cacagcgcct tggctcctcc agtgtgaggc caggcttgta
     9241 ccactactgc ttcatggccc cgtacaccca cttcacccag gccctcgctg acgccagcct
     9301 gaggaacatg gtgcaggcgg agcaggagaa cgacacctcg ggatggtttg atgtcctcca
     9361 gaaagtgtct acccagttga agacaaacct cacgagtgtc acaaagaacc gtgcagataa
     9421 gaatgctatt cataatcaca ttcgtttgtt tgaacctctt gttataaaag ctttaaaaca
     9481 gtacacgact acaacatgtg tgcagttaca gaagcaggtt ttagatttgc tggcgcagct
     9541 ggttcagtta cgggttaatt actgtcttct ggattcagat caggtgttta ttggctttgt
     9601 attgaaacag tttgaataca ttgaagtggg ccagttcagg gaatcagagg caatcattcc
     9661 aaacatcttt ttcttcttgg tattactatc ttatgaacgc tatcattcaa aacagatcat
     9721 tggaattcct aaaatcattc agctctgtga tggcatcatg gccagtggaa ggaaggctgt
     9781 gacacatgcc ataccggctc tgcagcccat agtccacgac ctctttgtat taagaggaac
     9841 aaataaagct gatgcaggaa aagagcttga aacccaaaaa gaggtggtgg tgtcaatgtt
     9901 actgagactc atccagtacc atcaggtgtt ggagatgttc attcttgtcc tgcagcagtg
     9961 ccacaaggag aatgaagaca agtggaagcg actgtctcga cagatagctg acatcatcct
    10021 cccaatgtta gccaaacagc agatgcacat tgactctcat gaagcccttg gagtgttaaa
    10081 tacattattt gagattttgg ccccttcctc cctccgtccg gtagacatgc ttttacggag
    10141 tatgttcgtc actccaaaca caatggcgtc cgtgagcact gttcaactgt ggatatcggg
    10201 aattctggcc attttgaggg ttctgatttc ccagtcaact gaagatattg ttctttctcg
    10261 tattcaggag ctctccttct ctccgtattt aatctcctgt acagtaatta ataggttaag
    10321 agatggggac agtacttcaa cgctagaaga acacagtgaa gggaaacaaa taaagaattt
    10381 gccagaagaa acattttcaa ggtttctatt acaactggtt ggtattcttt tagaagacat
    10441 tgttacaaaa cagctgaagg tggaaatgag tgagcagcaa catactttct attgccagga
    10501 actaggcaca ctgctaatgt gtctgatcca catcttcaag tctggaatgt tccggagaat
    10561 cacagcagct gccactaggc tgttccgcag tgatggctgt ggcggcagtt tctacaccct
    10621 ggacagcttg aacttgcggg ctcgttccat gatcaccacc cacccggccc tggtgctgct
    10681 ctggtgtcag atactgctgc ttgtcaacca caccgactac cgctggtggg cagaagtgca
    10741 gcagaccccg aaaagacaca gtctgtccag cacaaagtta cttagtcccc agatgtctgg
    10801 agaagaggag gattctgact tggcagccaa acttggaatg tgcaatagag aaatagtacg
    10861 aagaggggct ctcattctct tctgtgatta tgtctgtcag aacctccatg actccgagca
    10921 cttaacgtgg ctcattgtaa atcacattca agatctgatc agcctttccc acgagcctcc
    10981 agtacaggac ttcatcagtg ccgttcatcg gaactctgct gccagcggcc tgttcatcca
    11041 ggcaattcag tctcgttgtg aaaacctttc aactccaacc atgctgaaga aaactcttca
    11101 gtgcttggag gggatccatc tcagccagtc gggagctgtg ctcacgctgt atgtggacag
    11161 gcttctgtgc acccctttcc gtgtgctggc tcgcatggtc gacatccttg cttgtcgccg
    11221 ggtagaaatg cttctggctg caaatttaca gagcagcatg gcccagttgc caatggaaga
    11281 actcaacaga atccaggaat accttcagag cagcgggctc gctcagagac accaaaggct
    11341 ctattccctg ctggacaggt ttcgtctctc caccatgcaa gactcactta gtccctctcc
    11401 tccagtctct tcccacccgc tggacgggga tgggcacgtg tcactggaaa cagtgagtcc
    11461 ggacaaagac tggtacgttc atcttgtcaa atcccagtgt tggaccaggt cagattctgc
    11521 actgctggaa ggtgcagagc tggtgaatcg gattcctgct gaagatatga atgccttcat
    11581 gatgaactcg gagttcaacc taagcctgct agctccatgc ttaagcctag ggatgagtga
    11641 aatttctggt ggccagaaga gtgccctttt tgaagcagcc cgtgaggtga ctctggcccg
    11701 tgtgagcggc accgtgcagc agctccctgc tgtccatcat gtcttccagc ccgagctgcc
    11761 tgcagaaccg gcggcctact ggagcaagtt gaatgatctg tttggggatg ctgcactgta
    11821 tcagtccctg cccactctgg cccgggccct ggcacagtac ctggtggtgg tctccaaact
    11881 gcccagtcat ttgcaccttc ctcctgagaa agagaaggac attgtgaaat tcgtggtggc
    11941 aacccttgag gccctgtcct ggcatttgat ccatgagcag atcccgctga gtctggatct
    12001 ccaggcaggg ctggactgct gctgcctggc cctgcagctg cctggcctct ggagcgtggt
    12061 ctcctccaca gagtttgtga cccacgcctg ctccctcatc cactgtgtgc acttcatcct
    12121 ggaggccgtt gcagtgcagc ctggagagca gcttcttagt ccagaaagaa ggacaaatac
    12181 cccaaaagcc atcagcgagg aggaggagga agtagatcca aacacacaga atcctaagta
    12241 tatcactgca gcctgtgaga tggtggcaga aatggtggag tctctgcagt cggtgttggc
    12301 cttgggtcat aaaaggaata gcggcgtgcc ggcgtttctc acgccattgc tcaggaacat
    12361 catcatcagc ctggcccgcc tgccccttgt caacagctac acacgtgtgc ccccactggt
    12421 gtggaagctt ggatggtcac ccaaaccggg aggggatttt ggcacagcat tccctgagat
    12481 ccccgtggag ttcctccagg aaaaggaagt ctttaaggag ttcatctacc gcatcaacac
    12541 actaggctgg accagtcgta ctcagtttga agaaacttgg gccaccctcc ttggtgtcct
    12601 ggtgacgcag cccctcgtga tggagcagga ggagagccca ccagaagaag acacagagag
    12661 gacccagatc aacgtcctgg ccgtgcaggc catcacctca ctggtgctca gtgcaatgac
    12721 tgtgcctgtg gccggcaacc cagctgtaag ctgcttggag cagcagcccc ggaacaagcc
    12781 tctgaaagct ctcgacacca ggtttgggag gaagctgagc attatcagag ggattgtgga
    12841 gcaagagatt caagcaatgg tttcaaagag agagaatatt gccacccatc atttatatca
    12901 ggcatgggat cctgtccctt ctctgtctcc ggctactaca ggtgccctca tcagccacga
    12961 gaagctgctg ctgcagatca accccgagcg ggagctgggg agcatgagct acaaactcgg
    13021 ccaggtgtcc atacactccg tgtggctagg gaacagcatc acacccctga gggaggagga
    13081 atgggacgag gaagaggagg aggaggccga cgcccctgca ccttcgtcac cacccacgtc
    13141 tccagtcaac tccaggaaac accgggctgg agttgacatc cactcctgtt cgcagttttt
    13201 gcttgagttg tacagccgct ggatcctgcc gtccagctca gccaggagga ccccggccat
    13261 cctgatcagt gaggtggtca gatcccttct agtggtctca gacttgttca ccgagcgcaa
    13321 ccagtttgag ctgatgtatg tgacgctgac agaactgcga agggtgcacc cttcagaaga
    13381 cgagatcctc gctcagtacc tggtgcctgc cacctgcaag gcagctgccg tccttgggat
    13441 ggacaaggcc gtggcggagc ctgtcagccg cctgctggag agcacgctca ggagcagcca
    13501 cctgcccagc agggttggag ccctgcacgg catcctctat gtgctggagt gcgacctgct
    13561 ggacgacact gccaagcagc tcatcccggt catcagcgac tatctcctct ccaacctgaa
    13621 agggatcgcc cactgcgtga acattcacag ccagcagcac gtactggtca tgtgtgccac
    13681 tgcgttttac ctcattgaga actatcctct ggacgtaggg ccggaatttt cagcatcaat
    13741 aatacagatg tgtggggtga tgctgtctgg aagtgaggag tccaccccct ccatcattta
    13801 ccactgtgcc ctcagaggcc tggagcgcct cctgctctct gagcagctct cccgcctgga
    13861 tgcagaatcg ctggtcaagc tgagtgtgga cagagtgaac gtgcacagcc cgcaccgggc
    13921 catggcggct ctgggcctga tgctcacctg catgtacaca ggaaaggaga aagtcagtcc
    13981 gggtagaact tcagacccta atcctgcagc ccccgacagc gagtcagtga ttgttgctat
    14041 ggagcgggta tctgttcttt ttgataggat caggaaaggc tttccttgtg aagccagagt
    14101 ggtggccagg atcctgcccc agtttctaga cgacttcttc ccaccccagg acatcatgaa
    14161 caaagtcatc ggagagtttc tgtccaacca gcagccatac ccccagttca tggccaccgt
    14221 ggtgtataag gtgtttcaga ctctgcacag caccgggcag tcgtccatgg tccgggactg
    14281 ggtcatgctg tccctctcca acttcacgca gagggccccg gtcgccatgg ccacgtggag
    14341 cctctcctgc ttctttgtca gcgcgtccac cagcccgtgg gtcgcggcga tcctcccaca
    14401 tgtcatcagc aggatgggca agctggagca ggtggacgtg aaccttttct gcctggtcgc
    14461 cacagacttc tacagacacc agatagagga ggagctcgac cgcagggcct tccagtctgt
    14521 gcttgaggtg gttgcagccc caggaagccc atatcaccgg ctgctgactt gtttacgaaa
    14581 tgtccacaag gtcaccacct gcggtggaag cggtgactac aaagacgatg acgacaagta
    14641 aaacctctac ttccaatcgc accatcatca ccatcaccat caccaccatg attacaagga
    14701 tgacgacgat aagtgaggcg gccgccaact tgatatcaag cttatcgata atcaacctct
    14761 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
    14821 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
    14881 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
    14941 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
    15001 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
    15061 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
    15121 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
    15181 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
    15241 ccttccttcc cgcgggcgcc cccctcctca cggcgagcga agcttgtcga gaagtactag
    15301 aggatctcta gagcctgcag tctcgacaag cttgtcgaga agtactagag gatcataatc
    15361 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    15421 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    15481 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    15541 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg
    15601 cttgagccta ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt
    15661 tttaattttc gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc
    15721 cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc
    15781 ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg
    15841 tgacaaaccg tcatcttcgg ctacttt
//
