LOCUS       sequence-220503-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of gRNA targeting GALNT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen YH, Lorenzetti F, 
            Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall 
            HH
  TITLE     A validated gRNA library for CRISPR/Cas9 targeting of the human 
            glycosyltransferase genome.
  JOURNAL   Glycobiology. 2018 Jan 5. pii: 4791732. doi: 10.1093/glycob/cwx101.
  PUBMED    29315387
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Glycobiology. 2018 Jan 5. pii: 4791732. doi: 
            10.1093/glycob/cwx101."
COMMENT     Sequence Label: gh1
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     104..123
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        203..278
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(417..434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(607..626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
       61 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
      121 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
      181 cgtcccactg tacactcaca atgttttaga gctagaaata gcaagttaaa ataaggctag
      241 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttggtac ctggagcaca
      301 agactggcct catgggcctt ccgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      361 cagctgcatt aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg
      421 ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag
      481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      721 atagctcacg ctgtaggtat ctcagttcgg tgta
//
