LOCUS       sequence-220504-        9974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized enAsBE1.2,
            also called RTW1348.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/
            D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)
FEATURES             Location/Qualifiers
     source          1..9974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..660
                     /label=chicken beta-actin promoter
     intron          661..1677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1685..1704
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1770..1779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1782..1802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1812..1832
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1842..2525
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2622..6539
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             6540..6587
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6624..6872
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6885..6905
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6995..7014)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7060..7115
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7114..7133)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7476..7492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7476..7492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7489..7511)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7500..7516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7524..7530,7531..7548,7549..7554))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7569..7590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7649..7844
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7695..7830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7850..7984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7887..7906)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7941..7960
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8052..8069)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8223..8811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8303..8322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8982..9773,9774..9842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9605..9624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9843..9947)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ctgcagtcga
     1741 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggg cccaaagaaa aagaggaaag
     1801 tcggcagtgg acctaaaaag aaacgaaagg ttgggtcagg tagctcagag actggcccag
     1861 tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag gtattcttcg
     1921 atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg gggggccggc
     1981 actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc aacttcatcg
     2041 agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt acctggtttc
     2101 tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg tcaaggtatc
     2161 cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac ccccgcaatc
     2221 gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg actgagcagg
     2281 agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa gcccactggc
     2341 ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc atcatactgg
     2401 gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca ttctttacca
     2461 tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg gccaccgggt
     2521 tgaaatctgg tggttcttct ggtggttcta gcggcagcga gactcccggg acctcagagt
     2581 ccgccacacc cgaaagttcc ggagggagta gcggcgggtc tacacagttc gagggcttta
     2641 ccaacctgta tcaggtgagc aagacactgc ggtttgagct gatcccacag ggcaagaccc
     2701 tgaagcacat ccaggagcag ggcttcatcg aggaggacaa ggcccgcaat gatcactaca
     2761 aggagctgaa gcccatcatc gatcggatct acaagaccta tgccgaccag tgcctgcagc
     2821 tggtgcagct ggattgggag aacctgagcg ccgccatcga ctcctataga aaggagaaaa
     2881 ccgaggagac aaggaacgcc ctgatcgagg agcaggccac atatcgcaat gccatccacg
     2941 actacttcat cggccggaca gacaacctga ccgatgccat caataagaga cacgccgaga
     3001 tctacaaggg cctgttcaag gccgagctgt ttaatggcaa ggtgctgaag cagctgggca
     3061 ccgtgaccac aaccgagcac gagaacgccc tgctgcggag cttcgacaag tttacaacct
     3121 acttctccgg cttttataga aacaggaaga acgtgttcag cgccgaggat atcagcacag
     3181 ccatcccaca ccgcatcgtg caggacaact tccccaagtt taaggagaat tgtcacatct
     3241 tcacacgcct gatcaccgcc gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg
     3301 ccatcggcat cttcgtgagc acctccatcg aggaggtgtt ttccttccct ttttataacc
     3361 agctgctgac acagacccag atcgacctgt ataaccagct gctgggagga atctctcggg
     3421 aggcaggcac cgagaagatc aagggcctga acgaggtgct gaatctggcc atccagaaga
     3481 atgatgagac agcccacatc atcgcctccc tgccacacag attcatcccc ctgtttaagc
     3541 agatcctgtc cgataggaac accctgtctt tcatcctgga ggagtttaag agcgacgagg
     3601 aagtgatcca gtccttctgc aagtacaaga cactgctgag aaacgagaac gtgctggaga
     3661 cagccgaggc cctgtttaac gagctgaaca gcatcgacct gacacacatc ttcatcagcc
     3721 acaagaagct ggagacaatc agcagcgccc tgtgcgacca ctgggataca ctgaggaatg
     3781 ccctgtatga gcggagaatc tccgagctga caggcaagat caccaagtct gccaaggaga
     3841 aggtgcagcg cagcctgaag cacgaggata tcaacctgca ggagatcatc tctgccgcag
     3901 gcaaggagct gagcgaggcc ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg
     3961 ccgccctgga tcagccactg cctacaaccc tgaagaagca ggaggagaag gagatcctga
     4021 agtctcagct ggacagcctg ctgggcctgt accacctgct ggactggttt gccgtggatg
     4081 agtccaacga ggtggacccc gagttctctg cccggctgac cggcatcaag ctggagatgg
     4141 agccttctct gagcttctac aacaaggcca gaaattatgc caccaagaag ccctactccg
     4201 tggagaagtt caagctgaac tttcagatgc ctacactggc cagaggctgg gacgtgaata
     4261 gagagaagaa caatggcgcc atcctgtttg tgaagaacgg cctgtactat ctgggcatca
     4321 tgccaaagca gaagggcagg tataaggccc tgagcttcga gcccacagag aaaaccagcg
     4381 agggctttga taagatgtac tatgactact tccctgatgc cgccaagatg atcccaaagt
     4441 gcagcaccca gctgaaggcc gtgacagccc actttcagac ccacacaacc cccatcctgc
     4501 tgtccaacaa tttcatcgag cctctggaga tcacaaagga gatctacgac ctgaacaatc
     4561 ctgagaagga gccaaagaag tttcagacag cctacgccaa gaaaaccggc gaccagaagg
     4621 gctacagaga ggccctgtgc aagtggatcg acttcacaag ggattttctg tccaagtata
     4681 ccaagacaac ctctatcgat ctgtctagcc tgcggccatc ctctcagtat aaggacctgg
     4741 gcgagtacta tgccgagctg aatcccctgc tgtaccacat cagcttccag agaatcgccg
     4801 agaaggagat catggatgcc gtggagacag gcaagctgta cctgttccag atctataaca
     4861 aggactttgc caagggccac cacggcaagc ctaatctgca cacactgtat tggaccggcc
     4921 tgttttctcc agagaacctg gccaagacaa gcatcaagct gaatggccag gccgagctgt
     4981 tctaccgccc taagtccagg atgaagagga tggcacaccg gctgggagag aagatgctga
     5041 acaagaagct gaaggatcag aaaaccccaa tccccgacac cctgtaccag gagctgtacg
     5101 actatgtgaa tcacagactg tcccacgacc tgtctgatga ggccagggcc ctgctgccca
     5161 acgtgatcac caaggaggtg tctcacgaga tcatcaagga taggcgcttt accagcgaca
     5221 agttcttttt ccacgtgcct atcacactga actatcaggc cgccaattcc ccatctaagt
     5281 tcaaccagag ggtgaatgcc tacctgaagg agcaccccga gacacctatc atcggcatcg
     5341 cccggggcga gagaaacctg atctatatca cagtgatcga ctccaccggc aagatcctgg
     5401 agcagcggag cctgaacacc atccagcagt ttgattacca gaagaagctg gacaacaggg
     5461 agaaggagag ggtggcagca aggcaggcct ggtctgtggt gggcacaatc aaggatctga
     5521 agcagggcta tctgagccag gtcatccacg agatcgtgga cctgatgatc cactaccagg
     5581 ccgtggtggt gctggagaac ctgaatttcg gctttaagag caagaggacc ggcatcgccg
     5641 agaaggccgt gtaccagcag ttcgagaaga tgctgatcga taagctgaat tgcctggtgc
     5701 tgaaggacta tccagcagag aaagtgggag gcgtgctgaa cccataccag ctgacagacc
     5761 agttcacctc ctttgccaag atgggcaccc agtctggctt cctgttttac gtgcctgccc
     5821 catatacatc taagatcgat cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca
     5881 tcaagaatca cgagagccgc aagcacttcc tggagggctt cgactttctg cactacgacg
     5941 tgaaaaccgg cgacttcatc ctgcacttta agatgaacag aaatctgtcc ttccagaggg
     6001 gcctgcccgg ctttatgcct gcatgggata tcgtgttcga gaagaacgag acacagtttg
     6061 acgccaaggg cacccctttc atcgccggca agagaatcgt gccagtgatc gagaatcaca
     6121 gattcaccgg cagataccgg gacctgtatc ctgccaacga gctgatcgcc ctgctggagg
     6181 agaagggcat cgtgttcagg gatggctcca acatcctgcc aaagctgctg gagaatgacg
     6241 attctcacgc catcgacacg atggtggccc tgatccgcag cgtgctgcag atgcggaact
     6301 ccaatgccgc cacaggcgag gactatatca acagccccgt gcgcgatctg aatggcgtgt
     6361 gcttcgactc ccggtttcag aacccagagt ggccaatgga cgccgatgcc aatggcgcct
     6421 accacatcgc cctgaagggc cagctgctgc tgaatcacct gaaggagagc aaggatctga
     6481 agctgcagaa cggcatctcc aatcaggact ggctggccta catccaggag ctgcgcaaca
     6541 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga tcctctggtg
     6601 gttctggagg atctggtggt tctactaatc tgtcagatat tattgaaaag gagaccggta
     6661 agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa gaagtcattg
     6721 ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc accgacgaga
     6781 atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg gtcatacagg
     6841 atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag aagaagagga
     6901 aagtctaaag cggccgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     6961 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     7021 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     7081 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     7141 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     7201 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     7261 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     7321 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     7381 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     7441 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7501 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7561 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7621 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     7681 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7801 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     7861 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7921 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7981 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8041 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8101 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8161 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8221 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8281 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8341 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8401 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8461 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8521 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8581 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8641 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8701 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8761 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8821 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8881 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8941 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9001 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9061 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9121 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9181 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9241 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9301 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9361 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9421 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9481 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9541 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9601 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9661 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9721 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9781 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9841 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9901 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9961 aaagtgccac ctgg
//
