LOCUS       sequence-220512-       11196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to dominant 
            negative Dynamin2a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 11196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-Dnm2a-DN
FEATURES             Location/Qualifiers
     source          1..11196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3020..3040)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3048..3064)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3080..3214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3104..3123)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3158..3177
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(3653..3840,3841..3852)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3886..3902)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3886..3902)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3939..3959)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3939..3957)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3978..3994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3991..4013)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4002..4018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4026..4032,4033..4050,4051..4056))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4071..4092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4209..4226)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4380..4968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4460..4479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5139..5930,5931..5999))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5762..5781
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6000..6104)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6267..6288)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6637..6648,6649..6811)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     7184..7200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7220..7240
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggctggcaac cgggggatgg aggaattgat
      781 ccccctgatc aataaactcc aagatgcttt cagctccatt ggccaaagtt gtaacctgga
      841 tctgccgcag atagcagttg tcggaggaca gagcgccgga gctagctccg tgctggaaaa
      901 tttcgtcggc agggatttcc ttccgcgagg ctcaggcatc gtcacccgca ggcctctcat
      961 tctgcagcta gtaaacaaca aagccgaata tgcagagttc ctacactgca aagggaggaa
     1021 gttcgtggac tttgatgaag tccggcagga gattgaagca gaaaccgaca ggattacagg
     1081 ctccaataag ggcatctctc ctatccccat caacctgaga gtctactctc ctaacgtatt
     1141 gaatctgaca ctcatcgatt tgcctgggat gacgaaggtg gcagtgggcg accagccgcc
     1201 tgacatcgag caccagattc gggacatgat aatgcagttt atcaccaggg agagctgtct
     1261 aatccttgcc gtcaccccag ccaacatgga cttggccaat tcggatgccc tcaaggtggc
     1321 gaaagaagtg gaccctcaag gactgcgtac cattggtgta atcactaaac tggatttaat
     1381 ggatgagggt acagatgccc gggatatcct ggaaaacaaa cttttaccac ttcgtagagg
     1441 ttatatcggt gtagtgaacc ggagtcagaa agacattgat ggccgtaagg acattcgtgc
     1501 tgcgctggca gcagaaagga agtttttcct gtcccaccct agttacagac atatggctga
     1561 aaggatggga acgccacacc tgcaaaaagc cctcaaccag caattgacca accacatccg
     1621 ggacacactt cccggcttgc ggagcaaact ccagtctcag cttctctctc tagaaaaaga
     1681 agtggaagag tataagaact tccgcccaga tgaccctact cgaaagacca aggccctgct
     1741 gcagatggtc cagcaatttg gtgtggactt tgagaagtgc attgagggat ccggagatca
     1801 ggtggacact gcagagctgt ctggaggtgc caggattaac cgaatctttc atgagcgctt
     1861 cccctttgag ctggtcaaga tcgtgtttga tgaaaaggag ctccggagag aaattagtca
     1921 cgccattaag aacgtccatg gtgtcagaac tgggctgttc actccagatc tggcgtttga
     1981 agccatcgtg aaaaagcaga tcattaagct gaaagagccc tgtctgaaat gtatcgatct
     2041 ggttattcag gagctcatca acacattcag gcagtgcacc aacaagttaa gctcgtaccc
     2101 tagactccga gaagagacgg agaggatcgt cactacttac gtcagagaga gagaagggaa
     2161 gaccaaggac caggttcttt tgctgattga cactgagctg tcatacatca acaccaacca
     2221 cgaggacttc attggatttg caaatgcaca gcaaaggaat atcgtcaaca agaagagggc
     2281 cattcccaac caggtgatcc gtaaaggctg gttaaccctg aacatcagca tcatgaaggg
     2341 aggttcaaag gagtactggt tcgtcctgac tgccgaatcg ctgtcctggt acaaagacga
     2401 ggaggaaaaa gagaagaaat acatgcttcc tctggataac ctgaagctca gagacgtgga
     2461 gaaaggcttc atgtccacta agcacatttt tgcaatcttc aacactgagc aaaggaatgt
     2521 gtataaggac ttgcgtcaga tcgagctggc ctgtgactcg caggaggaca tggatagttg
     2581 gaaagcctct tttctcagag caggcgttta ccctgagaaa gatcaagtgg agtcagaaga
     2641 ggcagcccca gccgacagct tctccatgga ccctcagttg gaaagacagg tcgagaccat
     2701 ccgtaacctg gtggactcct atattggaat cgtcaacaaa accatcagag acctcatgcc
     2761 caagactatc atgcatctca tgatcaacag cgctaaagac ttcatccact cagagctgct
     2821 ggcgtacctg tactcgtctg gagatcagaa cagtctgatg gaggagtctg cagaccaggc
     2881 ccagcgcaga gacgagatgc tgaggatgta ccatgccatc aaagaggcac tcagcatcat
     2941 cggtgacatc agcaccagca ccatttccac acctgtgcca ccacccgtca acgacagctg
     3001 gatacctgaa accaggtgac aactttatta tacatagttg ataattcact ggccgtcgtt
     3061 ttacggtacc atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac
     3121 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     3181 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     3241 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     3301 tggtatggct gattatgatc ctctagatca gatctgcgaa gatacggcca cgggtgctct
     3361 tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc
     3421 tccattttct tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt
     3481 gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac
     3541 caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac
     3601 tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc
     3661 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
     3721 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
     3781 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
     3841 tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta
     3901 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     3961 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4021 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4081 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4141 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4201 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4261 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4321 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4801 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4921 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4981 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg cattttggtc
     5041 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5101 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5161 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5221 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5281 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5341 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5401 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5461 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5521 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5581 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5641 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5701 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5761 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5821 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5881 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5941 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6001 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6061 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6121 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     6181 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     6241 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     6301 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca cgctggctaa
     6361 gaactcatca gcctccccgg tccatctacc cacgtaccaa tgcaccaatt ggccacaatg
     6421 acggctacta catggtgcca ttccttcctc tttataggaa tggagactac ctcctgtcca
     6481 acaatgctct tggatacgag tacgcctacc tgttggaccc aggtcattgc acaacaccag
     6541 aaatgccctc tgatctgcaa aagacgtgaa tatctgttca gacacccata tccactctgt
     6601 tccacacagg tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa
     6661 attttactca agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag
     6721 tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg
     6781 agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt
     6841 taatctcaaa aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt
     6901 gtatttaatt atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt
     6961 gtaaaacctc agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct
     7021 agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga
     7081 tgcgggaaga ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag
     7141 agactccctg gtgtctgaaa cacaggccag atgggccctc gagcaggaaa cagctatgac
     7201 catgattacg ccaagctatc aactttgtat agaaaagttg gcacgcgcag attccgtgtg
     7261 ggcctggtaa tatcttacca ttttaagcta ttttaagatt agggaaaaaa attaaataaa
     7321 actactttaa aattactttc cttaaaaagc aacatattta tttagttttt gtttaactgg
     7381 ctcaagattg taatatttta cttttacatg tttttttaag tacctaaatg tgttcaaaag
     7441 taaagtatgt tattgttttt tttttttaat gttagctaag ctttaaattg taatatatta
     7501 cttttaaaag tgacttttta aaaatgtaaa taaaaaagta actataaatg tgtaatgcat
     7561 gttatttctg tgggttttta attttcatct caactgggct tacaatttta acataataaa
     7621 aatcaaatca aatcaaatca ggcccttatt tgtcacatac actcttgtat actgaaataa
     7681 actaaatgtt gagaaaaatt taaatacacc aaaataacgc ataccttttt tgtaattacc
     7741 taagtaaaac atttagttcc ttttttaagt gactcaagat tgtaatctat taattgtaaa
     7801 agataaaact agtgatcaat ctgacgtgct tctatgcagc ctgtgagatt aattgtgatg
     7861 tttaattatt aaacaattta aatggatcaa acatttcaca tgtgcttatt gtgtccaaca
     7921 aaaccagcag aagcacttaa aaatattgca tgacaacatg agtttttaat gtaaattata
     7981 tatacttcct aaacgaagtt aatcatattt ctaaaataat aatgtcattg aaatatttaa
     8041 ataaatgata agctttagtt gattcaacat aaaattgaaa gcaataagga cttttttttt
     8101 ttacagcggt gaagcaaaag catgtaataa tcatataata taataaataa ataaataaat
     8161 aatggctctt ggtgcaaata tgaacacttt aaataaatat agaataaata tgatcaataa
     8221 gtcataacga tgtgttttat gttgattata atttaaacat gtttctaacg caagttttaa
     8281 aaactgtttt aaagtattat tttaagttat aagatcaatt tgactcaaca ttttgtagtg
     8341 aaaatatgtt gtgtaaaatt aatataatat acactataaa aatgcagggt tccacacaat
     8401 acattaatgt tgtcccaaca caaatcgatt aagttaacaa atatatgtgg attgaacata
     8461 aaaaattaag ttgtcccaat gaaatctcaa gaattgtgtt gtttcagctc attttaaata
     8521 agtagtttta acaagtaaaa aataacatat ttttgagtgt gtatatatat atattacaaa
     8581 atataataat ataaaatgta aaagatataa accatgctac atatatttaa gagctatttt
     8641 gcacattact tttttgcaag ccattttagt gtttttttta caaatatata ttagctaaat
     8701 atacaatatc ttaaatatac aattaatcag acatgcaatt aatgcattta acctacataa
     8761 aaatacatga tcaacatatg ttttatgctg attacaacta aatcaaacgt aatgaaatta
     8821 atttataaaa gcgatttaaa tatgtcaata ttttaagtta aaatcacttg aaacctacta
     8881 taaatgaact taaattaaaa aaatctcctt taattccaat gcaacagtgt tgttttttca
     8941 gctgaggacc cccggtgcag atggttggcc cccggcccgc tattctaagg aagcctttct
     9001 taattttagg gcggcgctgc atgcgctcag atcagattca cgggtggaga gggttatttt
     9061 ctctttttta ttttttcttg cctgctcata tttgggcctg tgtgaacccc aggctccgcc
     9121 tcggccccca ggagccgctc ttatttaaag gggatcctcg ccttcagtct tcgtcccagt
     9181 ggaagcatca gtcttgtgat aacaacttgg tgagtcctac agaccagcgc tcgactctag
     9241 gctttaaatg cattgatttc tctggattag ctaccgggag agttttaact ttgcttatta
     9301 gtcttgtgct cgaactgcta ggtttaaaga gtgttttctc taatgcatgc atgaagcgac
     9361 agctgaaaac cagaaataga gcgtttgtcc tcctgttaaa ctctagcctt tctttggcat
     9421 ttgtcagtcc gagtcttcag ctgccggcag gagattgggt ttcagtaaca cttgctgagc
     9481 acaaactaca cgtgctttct tcagatttaa ttcattatta atcattctag atttaaatga
     9541 tgttcttact cattgctgat tattttagcc ttgcggagca gattaaatga gcacgtgttt
     9601 cattaagatt gatttaaata taattaatat ttcaccactg tagttttaaa attgccttct
     9661 aagtcatggt tgatttttaa taggtatcgg ctctaattag attactattt tggtttttaa
     9721 atgttatttc tatttcattt gtaggctcgt ttttaatgtg attttattag attctagttt
     9781 taaaatcatc tcttagcctt aatgagcaga ctgaatgaac atgtttcatt aagattaact
     9841 tcattataat gaattattta atcattctag tgggttataa atcagctcta tttaaattaa
     9901 ttgtcgtgtt tatttctatt taatttttga ttattttttc agggtcgttt ttatgtgatt
     9961 ttttcccgtt tctaatttta aactaattgt ttgattttta tatgattcga ctctttttaa
    10021 atgaattgtt ttgctttttt tattgagcat atatatatta tacaatttca atcaacaatt
    10081 gttgtgcttt ttaaatgttg tttctatttc atttctaggg tcgattttat tcgattctag
    10141 ttttaaaatc gtgattttta acttctaacc ttactgagca gactgaatga acatgtttca
    10201 tcaagattaa cttcattata gttaattatt gaatcgtgct agtggtttat aaatcagctc
    10261 tatttaaatt aatcatcatc attatttcta tttaattttt attatttctt taggattatt
    10321 tttatgtgat ttttttcacg ttctagtttt aaactcaatg ctcattactg ctcacccttg
    10381 ctgagcagtc tttcgttaag attaaattaa ttaataacca ttctagtttt aaaacagtgt
    10441 cttaaggcat agctgatatt ttacaagttt actatttaat atatctcctt tcttttttta
    10501 gggtcgattt catctgattt gattaatttt tattatcttc actaagcttt ttgttctagg
    10561 ttttattctc agtattatta ctgttatatt tgaactgaat ttctcctcat agtttattca
    10621 ttttatttat ttattttttg tttattcgct attttgtatt ttagttttta attagaaatt
    10681 ttattagaat cgttttgaac tagtttttat gcactctaac attaatatta aatcataact
    10741 attattttta ttataattta tttgtgttta tctttttgtg tagctttcat gtgtttttat
    10801 tttagtttta ttatgtttgc attgaatttt ttggactagt ttatataacg atatacagaa
    10861 aagccatgac aacaaatgtt tttataatac ctattaagta taagctaatc agatttccac
    10921 tattctgttc aaagttaaca ttattagcca ttctgatgga tcatttgaca tttgtcatat
    10981 gtctttatga acactgctct ttcagatgcc acttttaaag ctgtttttta cagtaaatga
    11041 acgtttatta ttatgacatt atttcctaag gcctctagag tacagcagga tgtggcgtgt
    11101 gcattaccgt gtaattatgc tgactgtaaa tgtgcattta tagactgtca gcatgtctga
    11161 ctgtgcgctg ttgtgattgg tcctgcacag accaag
//
