LOCUS       sequence-220516-       11490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to dominant 
            negative Dynamin2b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 11490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-Dnm2b-DN
FEATURES             Location/Qualifiers
     source          1..11490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3314..3334)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3342..3358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3374..3508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3398..3417)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3452..3471
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(3947..4134,4135..4146)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4180..4196)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4180..4196)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4233..4253)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4233..4251)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4272..4288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4285..4307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4296..4312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4320..4326,4327..4344,4345..4350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4365..4386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4503..4520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4674..5262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4754..4773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5433..6224,6225..6293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6056..6075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6294..6398)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6561..6582)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(6931..6942,6943..7105)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     7478..7494
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7514..7534
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggggaac cgcgggatgg aggacttgat
      781 tcccctcatt aacaagctgc atgacgcctt cagctccatc ggccagagct gcaacctgga
      841 gctgccgcag atcgcggtgg tcggcgtgca gagtgccggc gcgacctccg tgctcgagaa
      901 cttcgtggga agggactttc tgccgagagg accaggaatc gtcactcgca ggcctctgat
      961 tctccagctg gtgaacagta aagctgaata tgccgagttc ctgcactgta agggccgcaa
     1021 gtttgtggac tttgacgagg ttcggatgga gattgaagca gaaacagacc gaatcactga
     1081 atctaacatt ggaatctctg cagtgcccat taacctgcgc ctttactcgc ccaacgtgct
     1141 gaatctgacg ctgatcgatc ttccgggcat aacgaaggtg gcggtgggtg accagccgca
     1201 ggatatcgag taccagatcc gggatatggt gttgcagttc atctccaaag agaactgtct
     1261 gattctggcc atcactccag caaacatgga cctgaccaac tctgacgctc tcaagatcag
     1321 caaagaggtg gaccctcatg gtctgcgcac catcggtgtg atcaccaaac tggacctgat
     1381 ggatgaagga accgacgcca gagacatcct ggagaacaag ctgctgccgc tgcgcagagg
     1441 ttatatcggt gtggtgaacc gcagtcagaa ggacattgat ggcaggaagg acatccgcgc
     1501 ggctctttct gctgagagga agttcttcct gtcccatcca gcatacagac acatagcaga
     1561 gagaatgggc acaccgtacc tgcagaaaac actcaaccag caactgatga accacatccg
     1621 cgacacactg ccggcgctgc gcagtaaact gcagtctcag ctgctgtcgc tggagaaaga
     1681 ggtggaggag tacaagaact tcaaaccaga cgatcctgcg cgcaaaacca aagccctgct
     1741 gcagatggtc cagcagttcg gtgtggactt tgagaagcgt atagggggct ctggagatca
     1801 ggtggacact ctggaactct ccggcggagc tcgaataaac cgcatcttcc acgagcgctt
     1861 ccccttcgag ctggtgaaga cggagtttga tgagaaggag ttgcggaggg agatcagtta
     1921 cgccatcaag aacattcacg gagtcaggac ggggctcttc accccagatc tggccttcga
     1981 ggccatcgtg aaaaagcagc tgataaagct gaaggagccg tgcctcaagt gcatcgacct
     2041 ggtcatccag gagctgatca acacggtcag gcagagcaca ctgaagctga agttgtatcc
     2101 tcggctgaga gaagagacgg agagaatcat cacaatatat gtcagagaga gagagaccaa
     2161 aaccaaagac cagatgatgc tgctgatcga tgtccagctc tcctacatca acacaaacca
     2221 cgaggacttc atcggattcg ccaatgcgca acagagcagc agagatgtca ctaagaagca
     2281 agccgttccc aatcaggtga tccgccgtgg ttggctgacc atcaacatca gcatcatgaa
     2341 gggcggctcc aaagactact ggttcgtgct gacggcggag tctctgtcct ggtacaagga
     2401 tgaggaggag aaggagaaga agttcatgct gccgctggat aacctgaagc tgcgggatgt
     2461 ggagaagagc ttcatgagca acaaacacac cttcggcatc ttcaacaccg agcagaggaa
     2521 cgtgtataaa gacctgcgtc agatcgagct ggcctgcgcc actcaggagg atgtggacag
     2581 ctggaaggcc tctttcctca gagcgggagt ctatcctgag aaagaccagg tggagaacgg
     2641 ggacgtgtca gcagactttc tctccatgga ccctcagctg gagcgtcagg ttgagaccat
     2701 cagtaatctg gtggactcgt acatcagcat cgtcaacaag agcatccgag acctgatgcc
     2761 caaaaccatc atgcacctga tgatcaacag cacgaaggag ttcatctact cggagctgct
     2821 ggcgtacctg tactcgtcag gtgatcagaa cagtctgatg gaggagtcgg cggagcagga
     2881 gcagcggcgg gacgagatgc tccgcatgta caacgctctg aaagaggctc tgaagatcat
     2941 cggggacgtc agcacatcca ccttcaccac acctgtgccc gcgccggtgg acgacacctg
     3001 gatacacact gaccccagtc ctccgtctca gcgtaagcca gctccgccca ctgtccgccc
     3061 tcctgcagtg cgaggctcca cccccggtgc tccagcccca ccttctctaa acccctcccc
     3121 tgccttcgga gctccgcctg ttccctccag acccaccaca ttggagccag ctgtagctcc
     3181 gcccccttgt gttccatctc gccctgcaca ggtcccgccc actgtgctgc ccgggatacg
     3241 cagtaggagg cctccagcgg ctccaaacag gcccacaatc atccgtccga cagaaccatc
     3301 tctgctggac tgacaacttt attatacata gttgataatt cactggccgt cgttttacgg
     3361 taccatcgat gatgatccag acatgataag atacattgat gagtttggac aaaccacaac
     3421 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3481 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3541 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     3601 ggctgattat gatcctctag atcagatctg cgaagatacg gccacgggtg ctcttgatcc
     3661 tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt
     3721 ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg
     3781 tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt
     3841 catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca
     3901 tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa
     3961 aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta
     4021 aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa
     4081 aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca
     4141 cctctggttc ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg
     4201 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     4261 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4321 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4381 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4441 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4501 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4561 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4621 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4681 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4741 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4801 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4861 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4921 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4981 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5041 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5101 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5161 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5221 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5281 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aaggcatttt ggtcatgaga
     5341 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5401 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5461 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5521 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5581 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5641 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5701 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5761 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5821 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5881 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5941 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6001 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6061 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6121 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6181 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6241 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6301 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6361 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6421 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     6481 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     6541 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6601 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccacgctgg ctaagaactc
     6661 atcagcctcc ccggtccatc tacccacgta ccaatgcacc aattggccac aatgacggct
     6721 actacatggt gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaatg
     6781 ctcttggata cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc
     6841 cctctgatct gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca
     6901 caggtcagag gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta
     6961 ctcaagtgaa agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg
     7021 gctagaatct tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt
     7081 aaggaagtaa aagtaaaagc aagaaagaaa actagagatt cttgtttaag cttttaatct
     7141 caaaaaacat taaatgaaat gcatacaagg ttttatcctg ctttagaact gtttgtattt
     7201 aattatcaaa ctataagaca gacaatctaa tgccagtaca cgctactcaa agttgtaaaa
     7261 cctcagattt aacttcagta gaagctgatt ctcaaaattg ttagtgtcaa gcctagctct
     7321 tttggggctg aaaagcaatc ctgcagtgct gaaaagcctc tcacaggcag ccgatgcggg
     7381 aagaggtgta ttagtcttga tagagaggct gcaaatagca ggaaacgtga gcagagactc
     7441 cctggtgtct gaaacacagg ccagatgggc cctcgagcag gaaacagcta tgaccatgat
     7501 tacgccaagc tatcaacttt gtatagaaaa gttggcacgc gcagattccg tgtgggcctg
     7561 gtaatatctt accattttaa gctattttaa gattagggaa aaaaattaaa taaaactact
     7621 ttaaaattac tttccttaaa aagcaacata tttatttagt ttttgtttaa ctggctcaag
     7681 attgtaatat tttactttta catgtttttt taagtaccta aatgtgttca aaagtaaagt
     7741 atgttattgt tttttttttt taatgttagc taagctttaa attgtaatat attactttta
     7801 aaagtgactt tttaaaaatg taaataaaaa agtaactata aatgtgtaat gcatgttatt
     7861 tctgtgggtt tttaattttc atctcaactg ggcttacaat tttaacataa taaaaatcaa
     7921 atcaaatcaa atcaggccct tatttgtcac atacactctt gtatactgaa ataaactaaa
     7981 tgttgagaaa aatttaaata caccaaaata acgcatacct tttttgtaat tacctaagta
     8041 aaacatttag ttcctttttt aagtgactca agattgtaat ctattaattg taaaagataa
     8101 aactagtgat caatctgacg tgcttctatg cagcctgtga gattaattgt gatgtttaat
     8161 tattaaacaa tttaaatgga tcaaacattt cacatgtgct tattgtgtcc aacaaaacca
     8221 gcagaagcac ttaaaaatat tgcatgacaa catgagtttt taatgtaaat tatatatact
     8281 tcctaaacga agttaatcat atttctaaaa taataatgtc attgaaatat ttaaataaat
     8341 gataagcttt agttgattca acataaaatt gaaagcaata aggacttttt ttttttacag
     8401 cggtgaagca aaagcatgta ataatcatat aatataataa ataaataaat aaataatggc
     8461 tcttggtgca aatatgaaca ctttaaataa atatagaata aatatgatca ataagtcata
     8521 acgatgtgtt ttatgttgat tataatttaa acatgtttct aacgcaagtt ttaaaaactg
     8581 ttttaaagta ttattttaag ttataagatc aatttgactc aacattttgt agtgaaaata
     8641 tgttgtgtaa aattaatata atatacacta taaaaatgca gggttccaca caatacatta
     8701 atgttgtccc aacacaaatc gattaagtta acaaatatat gtggattgaa cataaaaaat
     8761 taagttgtcc caatgaaatc tcaagaattg tgttgtttca gctcatttta aataagtagt
     8821 tttaacaagt aaaaaataac atatttttga gtgtgtatat atatatatta caaaatataa
     8881 taatataaaa tgtaaaagat ataaaccatg ctacatatat ttaagagcta ttttgcacat
     8941 tacttttttg caagccattt tagtgttttt tttacaaata tatattagct aaatatacaa
     9001 tatcttaaat atacaattaa tcagacatgc aattaatgca tttaacctac ataaaaatac
     9061 atgatcaaca tatgttttat gctgattaca actaaatcaa acgtaatgaa attaatttat
     9121 aaaagcgatt taaatatgtc aatattttaa gttaaaatca cttgaaacct actataaatg
     9181 aacttaaatt aaaaaaatct cctttaattc caatgcaaca gtgttgtttt ttcagctgag
     9241 gacccccggt gcagatggtt ggcccccggc ccgctattct aaggaagcct ttcttaattt
     9301 tagggcggcg ctgcatgcgc tcagatcaga ttcacgggtg gagagggtta ttttctcttt
     9361 tttatttttt cttgcctgct catatttggg cctgtgtgaa ccccaggctc cgcctcggcc
     9421 cccaggagcc gctcttattt aaaggggatc ctcgccttca gtcttcgtcc cagtggaagc
     9481 atcagtcttg tgataacaac ttggtgagtc ctacagacca gcgctcgact ctaggcttta
     9541 aatgcattga tttctctgga ttagctaccg ggagagtttt aactttgctt attagtcttg
     9601 tgctcgaact gctaggttta aagagtgttt tctctaatgc atgcatgaag cgacagctga
     9661 aaaccagaaa tagagcgttt gtcctcctgt taaactctag cctttctttg gcatttgtca
     9721 gtccgagtct tcagctgccg gcaggagatt gggtttcagt aacacttgct gagcacaaac
     9781 tacacgtgct ttcttcagat ttaattcatt attaatcatt ctagatttaa atgatgttct
     9841 tactcattgc tgattatttt agccttgcgg agcagattaa atgagcacgt gtttcattaa
     9901 gattgattta aatataatta atatttcacc actgtagttt taaaattgcc ttctaagtca
     9961 tggttgattt ttaataggta tcggctctaa ttagattact attttggttt ttaaatgtta
    10021 tttctatttc atttgtaggc tcgtttttaa tgtgatttta ttagattcta gttttaaaat
    10081 catctcttag ccttaatgag cagactgaat gaacatgttt cattaagatt aacttcatta
    10141 taatgaatta tttaatcatt ctagtgggtt ataaatcagc tctatttaaa ttaattgtcg
    10201 tgtttatttc tatttaattt ttgattattt tttcagggtc gtttttatgt gattttttcc
    10261 cgtttctaat tttaaactaa ttgtttgatt tttatatgat tcgactcttt ttaaatgaat
    10321 tgttttgctt tttttattga gcatatatat attatacaat ttcaatcaac aattgttgtg
    10381 ctttttaaat gttgtttcta tttcatttct agggtcgatt ttattcgatt ctagttttaa
    10441 aatcgtgatt tttaacttct aaccttactg agcagactga atgaacatgt ttcatcaaga
    10501 ttaacttcat tatagttaat tattgaatcg tgctagtggt ttataaatca gctctattta
    10561 aattaatcat catcattatt tctatttaat ttttattatt tctttaggat tatttttatg
    10621 tgattttttt cacgttctag ttttaaactc aatgctcatt actgctcacc cttgctgagc
    10681 agtctttcgt taagattaaa ttaattaata accattctag ttttaaaaca gtgtcttaag
    10741 gcatagctga tattttacaa gtttactatt taatatatct cctttctttt tttagggtcg
    10801 atttcatctg atttgattaa tttttattat cttcactaag ctttttgttc taggttttat
    10861 tctcagtatt attactgtta tatttgaact gaatttctcc tcatagttta ttcattttat
    10921 ttatttattt tttgtttatt cgctattttg tattttagtt tttaattaga aattttatta
    10981 gaatcgtttt gaactagttt ttatgcactc taacattaat attaaatcat aactattatt
    11041 tttattataa tttatttgtg tttatctttt tgtgtagctt tcatgtgttt ttattttagt
    11101 tttattatgt ttgcattgaa ttttttggac tagtttatat aacgatatac agaaaagcca
    11161 tgacaacaaa tgtttttata atacctatta agtataagct aatcagattt ccactattct
    11221 gttcaaagtt aacattatta gccattctga tggatcattt gacatttgtc atatgtcttt
    11281 atgaacactg ctctttcaga tgccactttt aaagctgttt tttacagtaa atgaacgttt
    11341 attattatga cattatttcc taaggcctct agagtacagc aggatgtggc gtgtgcatta
    11401 ccgtgtaatt atgctgactg taaatgtgca tttatagact gtcagcatgt ctgactgtgc
    11461 gctgttgtga ttggtcctgc acagaccaag
//
