LOCUS       sequence-220518-       10180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used along with I-SceI enzyme to generate transgenic zebrafish 
            expressing hepatocyte-specific Xenopus activated beta-catenin and 
            lens-specific Venus fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Evason KJ, Francisco MT, Juric V, Balakrishnan S, Lopez Pazmino Mdel
            P, Gordan JD, Kakar S, Spitsbergen J, Goga A, Stainier DY
  TITLE     Identification of Chemical Inhibitors of beta-Catenin-Driven Liver 
            Tumorigenesis in Zebrafish.
  JOURNAL   PLoS Genet. 2015 Jul 2;11(7):e1005305. doi: 
            10.1371/journal.pgen.1005305. eCollection 2015 Jul.
  PUBMED    26134322
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2015-07-2"; volume: "11(7):e1005305. doi"; pages: " 
            10.1371/journal.pgen.1005305. eCollection 2015 Jul"
COMMENT     Sequence Label: pKE4-I-SceI-fabp10a:Xpt-beta-cat, cryaa:Venus
FEATURES             Location/Qualifiers
     source          1..10180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3000..3035
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     polyA_signal    5598..5732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5635..5654)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5689..5708
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5750..5797
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    5821..6028
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6017..6034
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(6052..6765,6766..6768,6769..6771))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6097..6118)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6446..6465
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6705..6726
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7361..7381)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7361..7379)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7412..7429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7412..7428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7421..7443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7569..8024)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7706..7727)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7918..7937
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8051..8155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8156..8224,8225..9016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8374..8393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9187..9775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9676..9695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9929..9946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10063..10084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10099..10104,10105..10122,10123..10129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10137..10153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10142..10164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10161..10177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10161..10177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattacgcca agcgcgttag ggataacagg gtaatgcgcg cgtaatacga ctcactatag
       61 ggcgaattgg agctcggaat tcctgagcat cagaatgggg aaggaagagg atttcagtga
      121 ctttgaacga ggcatggttg ttgctgccag atgggctgct ctgagtattt cagaaactgc
      181 tgatcttcag ggattttcac gcacaaccat ctctagggtt tacagagaat gatctgaaaa
      241 gaggaaatat ccagtgagcg gcagttctgt gggtgcaaat gccttgttga tgccagagat
      301 cagaggagaa tggccagact ggttccagct gatagaaagg caacagtaac tcaaataagc
      361 actcgttaca accgagctct gcagaagagc atctctgaac acacaacacg tccaaccttg
      421 aggcagatgg gctacagcag cagaagacca caccgggtgc cgctcctgtc agctaagaac
      481 aggaaactga ggctacaatt cacacagact caccaaaact ggacaatata ttattagccc
      541 cccttagaat ttttcatttg ataatatttt tcttctggcg aaagcctcat ttgttttata
      601 tattatagaa taaaattagt ttttaatagt ttttatgcca ttttaaggtc aatattatta
      661 gcccctttaa gctatttttt ttcgatagtc tacagaacaa accatcggta tacaatgact
      721 tgcctaatta ccctaacctg cctagttacc ctaattaacc tagttaagcc tttaaatgtc
      781 actttaagct gtatagaagt gtcttgaaga atatctagtc taatattatt gactgtcatc
      841 atggcaaaga taaaataaat cagttattaa aactattatg attagaaatg tgctgaaaca
      901 atctgctctc cgataaacag aaattgaaca aaataaacag gggggctaat aaatttaagg
      961 ggttaaataa ttctgattgc aaaaaaaatg atgtctgcaa aactgttgca aataatttat
     1021 ttgtgttgat tttaagcaaa caaattaaat ttaataaaat acaacttaat ctgtttgttt
     1081 aaattcagcc ctaataaatt gtttacagcc acttaacgta aaaaaattga gtaaatccaa
     1141 ggaatcatct ctgaataatt tttttcagtg tatatatata tatatatatt cttacaaaac
     1201 aactcattta ctttagttta attttcaggg gcaaaaaact aaagtaatcg acgttgcttg
     1261 aataaaaagt gtaattaagg gaatgaggta acatttaacc atgtgtcaat gcagtttaaa
     1321 tatgccagtt agtggtatat gtttaaatgg taagctattc aaaactttaa actaacttaa
     1381 ccagcctttt gttgtcagac tgaacagact ttccatctgc attattagag actaatcttt
     1441 ggctggatga atgattcatc tgctgatatt tcagaataga cagattgagg ctgtttctaa
     1501 tatgattatg caacctgagg gtgattattt gaagcaaact ccacagacca gcaggtcatt
     1561 gaccgtcgtg tgttcaaaca gagcagaaac atttgcaaaa ctggtctgac aggagaatcc
     1621 agtccagcac aacacatatg ctgagcaaac tgaatcaatc ctgcaggtca actctcgtgc
     1681 tttaagttta ttaagattat tttatttatt tattatttta tttatctatt tatttattta
     1741 gttgtttatt tagcaattat ttgtttattt atttgtttat ttattcctgc agatcatgcc
     1801 ttgtgccttt ttacatttaa tttaattttt aatttaattt ccttttattt ttttttattt
     1861 ttttatttta ttttatttta cagtctgaca aatactgaac taaaaaccct cagatcatgt
     1921 cttatgcatt tcattttatt ttatttcatt ttatattatt aattttaata tttttatttt
     1981 acagtctgac aaatactgaa ttaaaaacca tcagatcatg tctcatgcat ttaacttaac
     2041 tttatttaat tcaattaaat tgtttgtttg tttgtttcct tgcatttgtt tgtttgtttt
     2101 ttacaatctg acatactgga ccgaaaaaac tcagatcatg tcttatgcat tttattttat
     2161 tttatttttt attttattgt ctattttatt ttatttcatc ttattttatt ttacagtctg
     2221 acatatggac caataaatct ctcagatcat gtctcatgca tttaatttaa tttttttatt
     2281 tattcatttt tatttattta ttttattatt tattttattt taatatttat tttattttat
     2341 tttattttat tttacagtct gacatactgg accaataaat ctctcagatc atgtctcatg
     2401 cattttactt ttattttatt agaattagaa agatcaaagg aacaactttt aaaatattaa
     2461 ttctgtatca aaatctcttt tgatacattt aattgattta aaaaagcagt tcacccaaga
     2521 aacatttcct cacagtcgaa tggttgtaaa cttttatgaa ttactttcac agaaaaagat
     2581 ttttggaaga atattggaaa aaaagcagcc attgacttcc atagtaacaa caaaaaatac
     2641 tatggaagtc aatggctgtt ttttcaccat tcggtatctt cattctggag cagaattttt
     2701 tgggtgatct gtccctttaa gtcgtcaaat cctggcgcaa tattccacat gcactgacga
     2761 gtctttattt ttggtctgct actgctgtgt gtgtgagggc atttacctca tcttctgctg
     2821 gagttgatga acggtgggtt gttcaaacag cagcaggtca ttgactgaac tcctctcgat
     2881 ataaaagctg cagatctgaa gctgaccttc actttgtgtt gagcggatct aattcgatat
     2941 caagcttatc gatgatggca actcaagcag atctaatgga gctggacatg gccatggagc
     3001 cagaccgaaa agcagcagtc agtcactggc agcaacagtc ttacctggat gctgggattc
     3061 atgctggagc aaccgccaca gcaccagctt tgagtggcaa aggaaaccca gaggatgaag
     3121 atgtggatac caaccaagtt ttgtatgagt gggagcaggg cttctctcag tccttcactc
     3181 aagatcaagt ggctgatatt gatggccagt acgccatgac aagagcccag cgagttcgtg
     3241 ctgcaatgtt tccagaaacc cttgatgaag gcatgcagat tccatccaca caatttgact
     3301 ctgcacaccc aacaaatgtg caacgtttag cagagccttc ccagatgctc aaacacgctg
     3361 tggtcaactt gatcaattac caggatgatg ctgaattggc cactcgagca atccccgagc
     3421 tgacgaaact gcttaatgac gaggaccagg ttgtagttaa caaggctgct gtaatggttc
     3481 accagctgtc aaagaaggaa gcctcacgcc atgctataat gcgctcacca cagatggtgt
     3541 ctgctatagt tcgtacaatg cagaacacaa atgatgtaga gactgcacga tgcactgcgg
     3601 gaacacttca caacctttct caccatcgtg aaggcttgtt ggccatattt aaatctggag
     3661 gcattcctgc tctagttaaa atgcttggct caccagttga ctctgtacta ttctatgcca
     3721 tcacaacact gcacaatcta cttctccatc aagaaggagc aaaaatggct gtacgtctgg
     3781 ctggtggctt gcagaaaatg gttgcccttc tcaataaaac taatgtcaag tttttggcaa
     3841 ttacaacaga ctgtcttcaa atattggcct atggaaatca agaaagcaag ttgataattc
     3901 ttgcaagtgg gggaccacaa gcgttggtaa atatcatgag gacttacagt tatgagaaac
     3961 ttctgtggac cacaagtcgg gtgcttaagg tgctatccgt gtgctctagc aacaagcctg
     4021 ctatagttga agctggtgga atgcaagctt taggactcca tctcacagac tcaagccaac
     4081 gtttggttca gaattgtctt tggacactaa gaaacctttc agatgcagca actaaacagg
     4141 agggaatgga aggtcttctt ggaaccctgg ttcagcttct tggatcagat gacattaatg
     4201 ttgtaacttg tgcagctggt atattgtcca acctcacctg caacaactac aaaaacaaaa
     4261 tgatggtttg tcaagttgga ggcattgaag cacttgttcg cacagttcta cgtgctgggg
     4321 atagagaaga cattactgaa cctgcaattt gtgctctgcg tcaccttacc agcaggcacc
     4381 aggaagcaga gatggctcaa aatgcagtac gtctccatta tggacttcca gtggtgatga
     4441 aactcttgca cccgccttca cactggcctt tgataaaggc tactgttggc ttgatccgca
     4501 atcttgccct ttgtcccgcc aatcatgctc cactgcgtga acagggtgcc attccccgac
     4561 ttgttcagtt gcttgttcgt gcacaccagg acacgcagag gcgcacttca atgggtggaa
     4621 cacaacagca gtttgtggag ggtgtccgca tggaggagat tgttgaaggc tgcacaggtg
     4681 ctcttcacat tttggctcgt gacatccaca accgaattgt aatcagaggt cttaacacca
     4741 ttccattatt tgtgcagttg ctgtactctc ctattgaaaa tatccaaaga gtggcagcag
     4801 gtgtgctgtg tgagctggcg caagacaaag aggctgctga agctattgaa gctgaaggtg
     4861 caactgctcc tcttactgag ctgcttcact ctagaaatga aggtgtcgca acttatgcag
     4921 ctgctgttct gttccgtatg tctgaggaca aaccccagga ctacaagaag cgtctgtcag
     4981 ttgagttgac cagctctctc ttcaggaccg agccaatgcc atggaatgag gctgcagacc
     5041 ttggtcttga tattggtgcc caaggcgaac ctcttggcta cagacaggat gattcaagct
     5101 accgatcttt ccatgctgct ggctatggtc aggatgcaat gggtatggac tccatgatgg
     5161 atcatgacat gggaggtcac cacccagggg cagactatcc agttgatgga cttcctgatt
     5221 tgagtcatgc ccaagatctt atggatggac tacctccagg tgatagcaac caactggcct
     5281 ggtttgacac tgacttgtaa gcggccgccg aaatgaccga ccaagcgacg cccaacctgc
     5341 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5401 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5461 accccccgga tctaagctct agataagtaa tgatcataat cagccatatc acatctgtag
     5521 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5581 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5641 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5701 aactcatcaa tgtatcttat catgtctgga tccgggggta ccgcgtcgag aagttcctat
     5761 tccgaagttc ctattctcta gaaagtatag gaacttcggg cccgcggtgg cggcgatctc
     5821 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
     5881 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
     5941 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
     6001 acagatggct ggcaactaga aggcacagtc gaggctgatc agcgggccgc tttacttgta
     6061 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     6121 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc
     6181 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     6241 cacgccgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
     6301 cttgtcggcg gtgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     6361 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     6421 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     6481 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     6541 gtcggggtag cgggcgaagc actgcaggcc gtagcccagg gtggtcacga gggtgggcca
     6601 gggcacgggc agcttgccgg tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc
     6661 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     6721 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgttgtcaga
     6781 cctggtaact ccttactgta acttattgca cctgatgaac aaaagatgtg aactgaacgc
     6841 aagtaggagg ctaatagagg ctagtacagt acaaagatcc ccaaatgatg tgtgccggcc
     6901 ttagtggagg cagacccctg gttatatacc tgtgctgcag aaagaggggc ttacaaatgg
     6961 atctctctgg aatggcatta tctcatggtg ggaacagtgt tgtcagcaga atcagcgggg
     7021 tggactctga cctgagaatg acagtctagc actgaatggc tcagacaata ctcttgtggc
     7081 caactatcac cggaaaataa gtgcctgcca aaacaaacca gaccagatga agctacatgg
     7141 ctgtagaacg tcagtattta atgtgttgtg gccacttggt tcaccagatt taagagctaa
     7201 tttttttaaa tatcacagat aatcaatcgc tttttttttt tcttgactga aaagcttaga
     7261 ctggaaaatt ctttgaaaaa gtttgcacta cagaaaataa atacataaca tcctgcactg
     7321 aatgcacact attaatgggc ccggtaccca gcttttgttc cctttagtga gggttaattg
     7381 cgcgctaggg ataacagggt aatacgcgct cactggccgt cgttttacaa cgtcgtgact
     7441 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     7501 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     7561 gcgaatggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     7621 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     7681 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     7741 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     7801 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     7861 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     7921 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     7981 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     8041 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8101 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8161 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8221 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8281 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8341 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8401 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8461 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8521 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8581 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8641 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8701 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8761 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8821 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8881 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8941 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9001 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9061 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9121 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9181 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9241 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9301 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9361 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9421 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9481 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9541 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9601 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9661 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9721 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9781 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9841 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9901 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9961 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    10021 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    10081 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    10141 gagcggataa caatttcaca caggaaacag ctatgaccat
//
