LOCUS       sequence-220519-       10310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to CD44a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-CD44a-mKate2
FEATURES             Location/Qualifiers
     source          1..10310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1209..1233)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1235..1930
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     polyA_signal    2052..2133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2134..2154)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2162..2178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2194..2328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2218..2237)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(2767..2954,2955..2966)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3000..3016)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3000..3016)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3053..3073)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3053..3071)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3092..3108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3105..3127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3116..3132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3140..3146,3147..3164,3165..3170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3185..3206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3323..3340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3494..4082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3574..3593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4253..5044,5045..5113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4876..4895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5114..5218)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5381..5402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5751..5762,5763..5925)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6298..6314
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6334..6354
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catgtggact ttattatttg tagtgtttgc
       61 tactggatca ccagctgtac ttcaggcagc cccggttcag ggtaacagtg ggagatgcag
      121 ttttgctggt gttttccaca tcgagggaat cagtcgttac tctcttacct ttcagaaggc
      181 tggcgaactg tgtcagagtt taggttacag acttgcaaca caagaacagg tcactgaagc
      241 ctataaaaaa ggcttgagaa cttgcagata cggctggatt gatggtcaaa atgtcacgtt
      301 cctcccacac attaatggtc caaactgtga ctccagctct gctgagatca ccttccatgc
      361 taaagcagga gaatttcttt ctgatgtcta ctgttttgac ccatcagatt catcaccaaa
      421 ctgtgatgat gttaaatctg aggatgttat gaatggaaac gcagccagag acagttcatt
      481 ctatgcaaaa ccacatgaag acttcattac agaattcaag acagaagggt ttttggtaga
      541 tgtggaagaa atattggaaa gagtaaaaag agaaacatct cttctgagtg acatgaaacc
      601 ctcaacagtg ccctctatga aaaaaattga aggcatcaag gaccctacaa gcaagcctca
      661 tattggaaag acccccagtg ttttgctatt tgatacagag ggcagtggat cgggattggt
      721 tgatcctgag cctagccatt tcacaacaag tccggttact acagagacag cgaagagtga
      781 tttaattgaa aacgttattg aagagaatga agagatggtg gttccagtag agacttcaaa
      841 tgtgaacccc attcaagtca aggaaccaag acgagtgaat gtattttcaa caacagaaag
      901 taatgcgaag gagatggttt cagatggatc gtcttctact tggatgataa ttactgcatt
      961 ctgtgtgttt attggagtca tagtatgcat atttgtggct attggcacta gagacaagtg
     1021 gtacggaccg agtaagagtg cagacatcac aactgaaaag aacaacaaag actacagtaa
     1081 aacagaaacg ctgccgctgt cagagaaaga gcaggagatt gtggcactga tgaatgttac
     1141 gaacatgaaa aatgggcaaa cagtggacat aacagctgca gatgaacacg aaaaagaata
     1201 tttaatgcac ccagctttct tgtacaaagt ggctgtgagc gagctgatta aggagaacat
     1261 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
     1321 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
     1381 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
     1441 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
     1501 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     1561 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
     1621 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
     1681 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
     1741 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
     1801 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
     1861 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
     1921 gcacagatga gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     1981 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2041 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2101 caaatttcac aaataaagca tttttttcac tgccaacttt attatacata gttgataatt
     2161 cactggccgt cgttttacgg taccatcgat gatgatccag acatgataag atacattgat
     2221 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2281 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2341 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2401 aacctctaca aatgtggtat ggctgattat gatcctctag atcagatctg cgaagatacg
     2461 gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg
     2521 aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca
     2581 ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa
     2641 tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa
     2701 catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat
     2761 agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     2821 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     2881 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     2941 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag
     3001 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     3061 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3301 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3901 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4141 aaggcatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5221 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     5281 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     5341 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     5401 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     5461 cccacgctgg ctaagaactc atcagcctcc ccggtccatc tacccacgta ccaatgcacc
     5521 aattggccac aatgacggct actacatggt gccattcctt cctctttata ggaatggaga
     5581 ctacctcctg tccaacaatg ctcttggata cgagtacgcc tacctgttgg acccaggtca
     5641 ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg tgaatatctg ttcagacacc
     5701 catatccact ctgttccaca caggtcagag gtttgtccag gagttcttga cagaggtgta
     5761 aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa attttactca
     5821 attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa agtactccat
     5881 taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa actagagatt
     5941 cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg ttttatcctg
     6001 ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa tgccagtaca
     6061 cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt ctcaaaattg
     6121 ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct gaaaagcctc
     6181 tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct gcaaatagca
     6241 ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc cctcgagcag
     6301 gaaacagcta tgaccatgat tacgccaagc tatcaacttt gtatagaaaa gttggcacgc
     6361 gcagattccg tgtgggcctg gtaatatctt accattttaa gctattttaa gattagggaa
     6421 aaaaattaaa taaaactact ttaaaattac tttccttaaa aagcaacata tttatttagt
     6481 ttttgtttaa ctggctcaag attgtaatat tttactttta catgtttttt taagtaccta
     6541 aatgtgttca aaagtaaagt atgttattgt tttttttttt taatgttagc taagctttaa
     6601 attgtaatat attactttta aaagtgactt tttaaaaatg taaataaaaa agtaactata
     6661 aatgtgtaat gcatgttatt tctgtgggtt tttaattttc atctcaactg ggcttacaat
     6721 tttaacataa taaaaatcaa atcaaatcaa atcaggccct tatttgtcac atacactctt
     6781 gtatactgaa ataaactaaa tgttgagaaa aatttaaata caccaaaata acgcatacct
     6841 tttttgtaat tacctaagta aaacatttag ttcctttttt aagtgactca agattgtaat
     6901 ctattaattg taaaagataa aactagtgat caatctgacg tgcttctatg cagcctgtga
     6961 gattaattgt gatgtttaat tattaaacaa tttaaatgga tcaaacattt cacatgtgct
     7021 tattgtgtcc aacaaaacca gcagaagcac ttaaaaatat tgcatgacaa catgagtttt
     7081 taatgtaaat tatatatact tcctaaacga agttaatcat atttctaaaa taataatgtc
     7141 attgaaatat ttaaataaat gataagcttt agttgattca acataaaatt gaaagcaata
     7201 aggacttttt ttttttacag cggtgaagca aaagcatgta ataatcatat aatataataa
     7261 ataaataaat aaataatggc tcttggtgca aatatgaaca ctttaaataa atatagaata
     7321 aatatgatca ataagtcata acgatgtgtt ttatgttgat tataatttaa acatgtttct
     7381 aacgcaagtt ttaaaaactg ttttaaagta ttattttaag ttataagatc aatttgactc
     7441 aacattttgt agtgaaaata tgttgtgtaa aattaatata atatacacta taaaaatgca
     7501 gggttccaca caatacatta atgttgtccc aacacaaatc gattaagtta acaaatatat
     7561 gtggattgaa cataaaaaat taagttgtcc caatgaaatc tcaagaattg tgttgtttca
     7621 gctcatttta aataagtagt tttaacaagt aaaaaataac atatttttga gtgtgtatat
     7681 atatatatta caaaatataa taatataaaa tgtaaaagat ataaaccatg ctacatatat
     7741 ttaagagcta ttttgcacat tacttttttg caagccattt tagtgttttt tttacaaata
     7801 tatattagct aaatatacaa tatcttaaat atacaattaa tcagacatgc aattaatgca
     7861 tttaacctac ataaaaatac atgatcaaca tatgttttat gctgattaca actaaatcaa
     7921 acgtaatgaa attaatttat aaaagcgatt taaatatgtc aatattttaa gttaaaatca
     7981 cttgaaacct actataaatg aacttaaatt aaaaaaatct cctttaattc caatgcaaca
     8041 gtgttgtttt ttcagctgag gacccccggt gcagatggtt ggcccccggc ccgctattct
     8101 aaggaagcct ttcttaattt tagggcggcg ctgcatgcgc tcagatcaga ttcacgggtg
     8161 gagagggtta ttttctcttt tttatttttt cttgcctgct catatttggg cctgtgtgaa
     8221 ccccaggctc cgcctcggcc cccaggagcc gctcttattt aaaggggatc ctcgccttca
     8281 gtcttcgtcc cagtggaagc atcagtcttg tgataacaac ttggtgagtc ctacagacca
     8341 gcgctcgact ctaggcttta aatgcattga tttctctgga ttagctaccg ggagagtttt
     8401 aactttgctt attagtcttg tgctcgaact gctaggttta aagagtgttt tctctaatgc
     8461 atgcatgaag cgacagctga aaaccagaaa tagagcgttt gtcctcctgt taaactctag
     8521 cctttctttg gcatttgtca gtccgagtct tcagctgccg gcaggagatt gggtttcagt
     8581 aacacttgct gagcacaaac tacacgtgct ttcttcagat ttaattcatt attaatcatt
     8641 ctagatttaa atgatgttct tactcattgc tgattatttt agccttgcgg agcagattaa
     8701 atgagcacgt gtttcattaa gattgattta aatataatta atatttcacc actgtagttt
     8761 taaaattgcc ttctaagtca tggttgattt ttaataggta tcggctctaa ttagattact
     8821 attttggttt ttaaatgtta tttctatttc atttgtaggc tcgtttttaa tgtgatttta
     8881 ttagattcta gttttaaaat catctcttag ccttaatgag cagactgaat gaacatgttt
     8941 cattaagatt aacttcatta taatgaatta tttaatcatt ctagtgggtt ataaatcagc
     9001 tctatttaaa ttaattgtcg tgtttatttc tatttaattt ttgattattt tttcagggtc
     9061 gtttttatgt gattttttcc cgtttctaat tttaaactaa ttgtttgatt tttatatgat
     9121 tcgactcttt ttaaatgaat tgttttgctt tttttattga gcatatatat attatacaat
     9181 ttcaatcaac aattgttgtg ctttttaaat gttgtttcta tttcatttct agggtcgatt
     9241 ttattcgatt ctagttttaa aatcgtgatt tttaacttct aaccttactg agcagactga
     9301 atgaacatgt ttcatcaaga ttaacttcat tatagttaat tattgaatcg tgctagtggt
     9361 ttataaatca gctctattta aattaatcat catcattatt tctatttaat ttttattatt
     9421 tctttaggat tatttttatg tgattttttt cacgttctag ttttaaactc aatgctcatt
     9481 actgctcacc cttgctgagc agtctttcgt taagattaaa ttaattaata accattctag
     9541 ttttaaaaca gtgtcttaag gcatagctga tattttacaa gtttactatt taatatatct
     9601 cctttctttt tttagggtcg atttcatctg atttgattaa tttttattat cttcactaag
     9661 ctttttgttc taggttttat tctcagtatt attactgtta tatttgaact gaatttctcc
     9721 tcatagttta ttcattttat ttatttattt tttgtttatt cgctattttg tattttagtt
     9781 tttaattaga aattttatta gaatcgtttt gaactagttt ttatgcactc taacattaat
     9841 attaaatcat aactattatt tttattataa tttatttgtg tttatctttt tgtgtagctt
     9901 tcatgtgttt ttattttagt tttattatgt ttgcattgaa ttttttggac tagtttatat
     9961 aacgatatac agaaaagcca tgacaacaaa tgtttttata atacctatta agtataagct
    10021 aatcagattt ccactattct gttcaaagtt aacattatta gccattctga tggatcattt
    10081 gacatttgtc atatgtcttt atgaacactg ctctttcaga tgccactttt aaagctgttt
    10141 tttacagtaa atgaacgttt attattatga cattatttcc taaggcctct agagtacagc
    10201 aggatgtggc gtgtgcatta ccgtgtaatt atgctgactg taaatgtgca tttatagact
    10261 gtcagcatgt ctgactgtgc gctgttgtga ttggtcctgc acagaccaag
//
