LOCUS       sequence-220521-       10370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to CD44b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-CD44b-mKate2
FEATURES             Location/Qualifiers
     source          1..10370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1269..1293)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1295..1990
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     polyA_signal    2112..2193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2194..2214)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2222..2238)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2254..2388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2278..2297)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(2827..3014,3015..3026)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3060..3076)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3060..3076)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3113..3133)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3113..3131)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3152..3168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3165..3187)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3176..3192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3200..3206,3207..3224,3225..3230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3245..3266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3383..3400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3554..4142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3634..3653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4313..5104,5105..5173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4936..4955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5174..5278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5441..5462)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5811..5822,5823..5985)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6358..6374
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6394..6414
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catgggctgg tcatgggtgt gtgttcagac
       61 gctccttcag ttgacaccct tcctctcttc tctcagtcag tcggactcaa tctctctaca
      121 ctcacagatg ctcttcagga cagagaggat gatgaagatg atgatgatga tggcagcact
      181 tctgtctgca ttgctgtcct cctccactgc aaacccaatc accggtgtgc gtgagcggag
      241 ctgcagctac tctggagtgt tccatgtgca gggtaaagag cgctacagcc tgacgtttga
      301 tgacgcacag agactgtgtg agcgtttgtc cactacatta gccaatctga cacaggtgaa
      361 gacagccttc aacagtggaa tggagacctg caggtatggc tggataaaca gctcacagct
      421 ggtgattctc cgtaaaacac caaatctaaa ctgtgctcgc aaccaaacag acgtcatcat
      481 aaagacttca gacgggaaaa aatatgacgc cttatgttat aatgataaag acaaatcaaa
      541 gaagaactgt acagaggcga ttgaccctgc aaaagtggac agcagttcat cttcagaagc
      601 cgcagtttct ccagagcgca tcactccaca ttactctaca gaagcagact cctccacagc
      661 agcagtttct ccagagcgca tcactccaca ttactctaca gacgcagatt cctccacatc
      721 agcagtttct ccagcccgct tcactccagg cggctctaca ggcgcagatt cctccacatc
      781 agcagaatct gctctgaaca cgtcagcagc agcagcagca gcagacgcag agagaaacac
      841 aactgaggga aacacgacac acaccagttc cccacacagc cacgaggagc ccaacgggac
      901 acgctcgacc cctcagacct ccacagaact gcagatcagc acgtctgcag cgcatcagga
      961 caccaccggc ccgggccgca ggcagaacac agtttctcca gagcgcatca ctccacacag
     1021 ccctacagaa gcagcttcct ccacatcagc aggtgcttct tcttctttaa cagactggct
     1081 ggtcatatta ctgacaatac tggcagttct tctcatctca ctcatctgtt gtatcgtagt
     1141 atacaggaaa aggctgtttg gacagaagca gactctggtc atcaacagca ctgtcgcagc
     1201 agcagagaac ggatcagcgg ctacacacac acaggagatg gtcacgctgc tgaacacaga
     1261 cacactccac ccagctttct tgtacaaagt ggctgtgagc gagctgatta aggagaacat
     1321 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
     1381 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
     1441 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
     1501 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
     1561 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     1621 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
     1681 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
     1741 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
     1801 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
     1861 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
     1921 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
     1981 gcacagatga gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     2041 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2101 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2161 caaatttcac aaataaagca tttttttcac tgccaacttt attatacata gttgataatt
     2221 cactggccgt cgttttacgg taccatcgat gatgatccag acatgataag atacattgat
     2281 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2341 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2401 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2461 aacctctaca aatgtggtat ggctgattat gatcctctag atcagatctg cgaagatacg
     2521 gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg
     2581 aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca
     2641 ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa
     2701 tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa
     2761 catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat
     2821 agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     2881 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     2941 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     3001 aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag
     3061 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     3121 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3181 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3241 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3301 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3361 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3961 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4201 aaggcatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4441 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4501 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4621 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4921 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5281 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     5341 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     5401 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     5461 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     5521 cccacgctgg ctaagaactc atcagcctcc ccggtccatc tacccacgta ccaatgcacc
     5581 aattggccac aatgacggct actacatggt gccattcctt cctctttata ggaatggaga
     5641 ctacctcctg tccaacaatg ctcttggata cgagtacgcc tacctgttgg acccaggtca
     5701 ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg tgaatatctg ttcagacacc
     5761 catatccact ctgttccaca caggtcagag gtttgtccag gagttcttga cagaggtgta
     5821 aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa attttactca
     5881 attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa agtactccat
     5941 taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa actagagatt
     6001 cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg ttttatcctg
     6061 ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa tgccagtaca
     6121 cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt ctcaaaattg
     6181 ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct gaaaagcctc
     6241 tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct gcaaatagca
     6301 ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc cctcgagcag
     6361 gaaacagcta tgaccatgat tacgccaagc tatcaacttt gtatagaaaa gttggcacgc
     6421 gcagattccg tgtgggcctg gtaatatctt accattttaa gctattttaa gattagggaa
     6481 aaaaattaaa taaaactact ttaaaattac tttccttaaa aagcaacata tttatttagt
     6541 ttttgtttaa ctggctcaag attgtaatat tttactttta catgtttttt taagtaccta
     6601 aatgtgttca aaagtaaagt atgttattgt tttttttttt taatgttagc taagctttaa
     6661 attgtaatat attactttta aaagtgactt tttaaaaatg taaataaaaa agtaactata
     6721 aatgtgtaat gcatgttatt tctgtgggtt tttaattttc atctcaactg ggcttacaat
     6781 tttaacataa taaaaatcaa atcaaatcaa atcaggccct tatttgtcac atacactctt
     6841 gtatactgaa ataaactaaa tgttgagaaa aatttaaata caccaaaata acgcatacct
     6901 tttttgtaat tacctaagta aaacatttag ttcctttttt aagtgactca agattgtaat
     6961 ctattaattg taaaagataa aactagtgat caatctgacg tgcttctatg cagcctgtga
     7021 gattaattgt gatgtttaat tattaaacaa tttaaatgga tcaaacattt cacatgtgct
     7081 tattgtgtcc aacaaaacca gcagaagcac ttaaaaatat tgcatgacaa catgagtttt
     7141 taatgtaaat tatatatact tcctaaacga agttaatcat atttctaaaa taataatgtc
     7201 attgaaatat ttaaataaat gataagcttt agttgattca acataaaatt gaaagcaata
     7261 aggacttttt ttttttacag cggtgaagca aaagcatgta ataatcatat aatataataa
     7321 ataaataaat aaataatggc tcttggtgca aatatgaaca ctttaaataa atatagaata
     7381 aatatgatca ataagtcata acgatgtgtt ttatgttgat tataatttaa acatgtttct
     7441 aacgcaagtt ttaaaaactg ttttaaagta ttattttaag ttataagatc aatttgactc
     7501 aacattttgt agtgaaaata tgttgtgtaa aattaatata atatacacta taaaaatgca
     7561 gggttccaca caatacatta atgttgtccc aacacaaatc gattaagtta acaaatatat
     7621 gtggattgaa cataaaaaat taagttgtcc caatgaaatc tcaagaattg tgttgtttca
     7681 gctcatttta aataagtagt tttaacaagt aaaaaataac atatttttga gtgtgtatat
     7741 atatatatta caaaatataa taatataaaa tgtaaaagat ataaaccatg ctacatatat
     7801 ttaagagcta ttttgcacat tacttttttg caagccattt tagtgttttt tttacaaata
     7861 tatattagct aaatatacaa tatcttaaat atacaattaa tcagacatgc aattaatgca
     7921 tttaacctac ataaaaatac atgatcaaca tatgttttat gctgattaca actaaatcaa
     7981 acgtaatgaa attaatttat aaaagcgatt taaatatgtc aatattttaa gttaaaatca
     8041 cttgaaacct actataaatg aacttaaatt aaaaaaatct cctttaattc caatgcaaca
     8101 gtgttgtttt ttcagctgag gacccccggt gcagatggtt ggcccccggc ccgctattct
     8161 aaggaagcct ttcttaattt tagggcggcg ctgcatgcgc tcagatcaga ttcacgggtg
     8221 gagagggtta ttttctcttt tttatttttt cttgcctgct catatttggg cctgtgtgaa
     8281 ccccaggctc cgcctcggcc cccaggagcc gctcttattt aaaggggatc ctcgccttca
     8341 gtcttcgtcc cagtggaagc atcagtcttg tgataacaac ttggtgagtc ctacagacca
     8401 gcgctcgact ctaggcttta aatgcattga tttctctgga ttagctaccg ggagagtttt
     8461 aactttgctt attagtcttg tgctcgaact gctaggttta aagagtgttt tctctaatgc
     8521 atgcatgaag cgacagctga aaaccagaaa tagagcgttt gtcctcctgt taaactctag
     8581 cctttctttg gcatttgtca gtccgagtct tcagctgccg gcaggagatt gggtttcagt
     8641 aacacttgct gagcacaaac tacacgtgct ttcttcagat ttaattcatt attaatcatt
     8701 ctagatttaa atgatgttct tactcattgc tgattatttt agccttgcgg agcagattaa
     8761 atgagcacgt gtttcattaa gattgattta aatataatta atatttcacc actgtagttt
     8821 taaaattgcc ttctaagtca tggttgattt ttaataggta tcggctctaa ttagattact
     8881 attttggttt ttaaatgtta tttctatttc atttgtaggc tcgtttttaa tgtgatttta
     8941 ttagattcta gttttaaaat catctcttag ccttaatgag cagactgaat gaacatgttt
     9001 cattaagatt aacttcatta taatgaatta tttaatcatt ctagtgggtt ataaatcagc
     9061 tctatttaaa ttaattgtcg tgtttatttc tatttaattt ttgattattt tttcagggtc
     9121 gtttttatgt gattttttcc cgtttctaat tttaaactaa ttgtttgatt tttatatgat
     9181 tcgactcttt ttaaatgaat tgttttgctt tttttattga gcatatatat attatacaat
     9241 ttcaatcaac aattgttgtg ctttttaaat gttgtttcta tttcatttct agggtcgatt
     9301 ttattcgatt ctagttttaa aatcgtgatt tttaacttct aaccttactg agcagactga
     9361 atgaacatgt ttcatcaaga ttaacttcat tatagttaat tattgaatcg tgctagtggt
     9421 ttataaatca gctctattta aattaatcat catcattatt tctatttaat ttttattatt
     9481 tctttaggat tatttttatg tgattttttt cacgttctag ttttaaactc aatgctcatt
     9541 actgctcacc cttgctgagc agtctttcgt taagattaaa ttaattaata accattctag
     9601 ttttaaaaca gtgtcttaag gcatagctga tattttacaa gtttactatt taatatatct
     9661 cctttctttt tttagggtcg atttcatctg atttgattaa tttttattat cttcactaag
     9721 ctttttgttc taggttttat tctcagtatt attactgtta tatttgaact gaatttctcc
     9781 tcatagttta ttcattttat ttatttattt tttgtttatt cgctattttg tattttagtt
     9841 tttaattaga aattttatta gaatcgtttt gaactagttt ttatgcactc taacattaat
     9901 attaaatcat aactattatt tttattataa tttatttgtg tttatctttt tgtgtagctt
     9961 tcatgtgttt ttattttagt tttattatgt ttgcattgaa ttttttggac tagtttatat
    10021 aacgatatac agaaaagcca tgacaacaaa tgtttttata atacctatta agtataagct
    10081 aatcagattt ccactattct gttcaaagtt aacattatta gccattctga tggatcattt
    10141 gacatttgtc atatgtcttt atgaacactg ctctttcaga tgccactttt aaagctgttt
    10201 tttacagtaa atgaacgttt attattatga cattatttcc taaggcctct agagtacagc
    10261 aggatgtggc gtgtgcatta ccgtgtaatt atgctgactg taaatgtgca tttatagact
    10321 gtcagcatgt ctgactgtgc gctgttgtga ttggtcctgc acagaccaag
//
