LOCUS       sequence-220535-       10401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to Pacsin2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10401)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 10401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-Pacsin2
FEATURES             Location/Qualifiers
     source          1..10401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             1235..1246
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(1815..1832)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(2225..2245)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2253..2269)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2285..2419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2309..2328)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2363..2382
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2858..3045,3046..3057)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3091..3107)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3091..3107)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3144..3164)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3144..3162)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3183..3199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3196..3218)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3207..3223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3231..3237,3238..3255,3256..3261))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3276..3297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3414..3431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3585..4173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3665..3684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4344..5135,5136..5204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4967..4986
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5205..5309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5472..5493)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5842..5853,5854..6016)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6389..6405
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6425..6445
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggcttcggat ttcaacgact ctctgattga
      781 cgtgtccagc gacagcttct gggaggtggg aaactacaag cgttcagtga agcgagtgga
      841 cgatgggaac cgtctgtgta acgacctgat gagctgcctg cacgagcgcg cacgcatcga
      901 gaagagctac agcacacagc tcaccgactg ggccaagcgc tggagacaga ccatcgagaa
      961 aggccctcag tacgggacgg tggagcgggc gtggtgcgct ctgatgaccg aggccgagaa
     1021 ggtgagcgat ctgcacatgg aggtgaaggc cgctctgatg gcggaggact tcgagaagat
     1081 caagaactgg cagaaggacg cctaccacaa gcagataatc gggggcttca aggagaccaa
     1141 agaggcggag gacggctttc gcaaggctca gaaaccctgg gccaagaagc tgaaggagat
     1201 ggagaccatg aagaaggcgt accacacggc ctgcaaagag gagaaaacag ccaccagcag
     1261 agagaactcc agcaaactgg acaacagcaa ccccgaagcc cagaagaagc tgcaggagaa
     1321 ggtggagaag tgccagcagg aggtgcagaa gacgaaggag cgctacgaga agtctctgga
     1381 agatctggac aaggtgacgc cgcagtacat ggagaacatg gagcaggtgt tcgagcagtg
     1441 gcagcagttc gagggaaaga gactgagctt cttcaaggag gtcctgctgg aggtcaagca
     1501 gcacctcgac ctctcctcca atcacaagta caccaccgtc taccacacgc tacaggacac
     1561 tatccaggga gcagatccac aagaggacct caagtggttc cgctcaaacc acggacccgg
     1621 catgaccatg aactggcccc agtttgagga atggtccatg gacctgaacc ggaccctcag
     1681 ccgccgggaa acgaagcgca gacctgctga gggagtgacc ctgacagcga tcagtcacaa
     1741 tcctgagcag agctccagag tcagcagtgt gcctgcggtc agtagttcag aagcggcatg
     1801 cttaaacccg tttgatgatg atgatgatga tgtagatgag gatgaagagc cggcggtcac
     1861 acacacccac agcagcccgg tcaaacatga ggaggctgta gcacaaactg cagcagtaga
     1921 gaaggctccc gctccagctg ccatcactgc tgctgatgat gatgatgatg aagagtcagg
     1981 aaaccccttc tcctcctcgt cagccaatgg gaatccgttt gaggaggagc cgtccacgga
     2041 gatgtgtgtg ccggtgcgcg ctgtctatga ctatgaggga caggagcagg acgagctcag
     2101 cttcaaagca ggagatgagc tggtgaagct gagtgaggag gacgagcagg gctggtgtaa
     2161 ggggcgtctg agcgacggca ctgttggtct gtatcctgcc aactatgtgg aggatctgca
     2221 gtgacaactt tattatacat agttgataat tcactggccg tcgttttacg gtaccatcga
     2281 tgatgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2341 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     2401 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     2461 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     2521 tgatcctcta gatcagatct gcgaagatac ggccacgggt gctcttgatc ctgtggctga
     2581 ttttggactg tgctgctcgc agctgctgat gaatcacata cttcctccat tttcttccac
     2641 tgattgactg ttataatttc cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag
     2701 atgtgacatg acgtcacttc caaaggacca atgaacatgt ctgaccaatt tcatataatg
     2761 tgaaaacgat tttcataggc agaataaata acatttaaat taaactgggc atcagcgcaa
     2821 ttcaattggt ttggtaatag caagggaaaa tagaatgaag tgatctccaa aaaataagta
     2881 ctttttgact gtaaataaaa ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt
     2941 aattaagtaa aagtaaaagt attgattttt aattgtactc aagtaaagta aaaatcccca
     3001 aaaataatac ttaagtacag taatcaagta aaattactca agtactttac acctctggtt
     3061 cttgaccccc taccttcagc aagcccagca gatccactag ttctagagcg gccgccaccg
     3121 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     3181 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     3241 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     3301 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     3361 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3421 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3481 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3601 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3661 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3721 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3781 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3841 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3901 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3961 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4021 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4081 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4201 tctgacgctc agtggaacga aaactcacgt taaggcattt tggtcatgag attatcaaaa
     4261 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4321 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4381 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4441 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4501 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4561 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4621 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4681 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4741 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4801 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4861 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4921 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4981 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5041 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5101 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5161 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5221 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5281 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     5341 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     5401 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     5461 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     5521 aagggcgaaa aaccgtctat cagggcgatg gcccacgctg gctaagaact catcagcctc
     5581 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
     5641 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaat gctcttggat
     5701 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
     5761 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
     5821 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
     5881 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
     5941 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
     6001 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
     6061 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
     6121 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
     6181 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     6241 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     6301 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     6361 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     6421 ctatcaactt tgtatagaaa agttggcacg cgcagattcc gtgtgggcct ggtaatatct
     6481 taccatttta agctatttta agattaggga aaaaaattaa ataaaactac tttaaaatta
     6541 ctttccttaa aaagcaacat atttatttag tttttgttta actggctcaa gattgtaata
     6601 ttttactttt acatgttttt ttaagtacct aaatgtgttc aaaagtaaag tatgttattg
     6661 tttttttttt ttaatgttag ctaagcttta aattgtaata tattactttt aaaagtgact
     6721 ttttaaaaat gtaaataaaa aagtaactat aaatgtgtaa tgcatgttat ttctgtgggt
     6781 ttttaatttt catctcaact gggcttacaa ttttaacata ataaaaatca aatcaaatca
     6841 aatcaggccc ttatttgtca catacactct tgtatactga aataaactaa atgttgagaa
     6901 aaatttaaat acaccaaaat aacgcatacc ttttttgtaa ttacctaagt aaaacattta
     6961 gttccttttt taagtgactc aagattgtaa tctattaatt gtaaaagata aaactagtga
     7021 tcaatctgac gtgcttctat gcagcctgtg agattaattg tgatgtttaa ttattaaaca
     7081 atttaaatgg atcaaacatt tcacatgtgc ttattgtgtc caacaaaacc agcagaagca
     7141 cttaaaaata ttgcatgaca acatgagttt ttaatgtaaa ttatatatac ttcctaaacg
     7201 aagttaatca tatttctaaa ataataatgt cattgaaata tttaaataaa tgataagctt
     7261 tagttgattc aacataaaat tgaaagcaat aaggactttt tttttttaca gcggtgaagc
     7321 aaaagcatgt aataatcata taatataata aataaataaa taaataatgg ctcttggtgc
     7381 aaatatgaac actttaaata aatatagaat aaatatgatc aataagtcat aacgatgtgt
     7441 tttatgttga ttataattta aacatgtttc taacgcaagt tttaaaaact gttttaaagt
     7501 attattttaa gttataagat caatttgact caacattttg tagtgaaaat atgttgtgta
     7561 aaattaatat aatatacact ataaaaatgc agggttccac acaatacatt aatgttgtcc
     7621 caacacaaat cgattaagtt aacaaatata tgtggattga acataaaaaa ttaagttgtc
     7681 ccaatgaaat ctcaagaatt gtgttgtttc agctcatttt aaataagtag ttttaacaag
     7741 taaaaaataa catatttttg agtgtgtata tatatatatt acaaaatata ataatataaa
     7801 atgtaaaaga tataaaccat gctacatata tttaagagct attttgcaca ttactttttt
     7861 gcaagccatt ttagtgtttt ttttacaaat atatattagc taaatataca atatcttaaa
     7921 tatacaatta atcagacatg caattaatgc atttaaccta cataaaaata catgatcaac
     7981 atatgtttta tgctgattac aactaaatca aacgtaatga aattaattta taaaagcgat
     8041 ttaaatatgt caatatttta agttaaaatc acttgaaacc tactataaat gaacttaaat
     8101 taaaaaaatc tcctttaatt ccaatgcaac agtgttgttt tttcagctga ggacccccgg
     8161 tgcagatggt tggcccccgg cccgctattc taaggaagcc tttcttaatt ttagggcggc
     8221 gctgcatgcg ctcagatcag attcacgggt ggagagggtt attttctctt ttttattttt
     8281 tcttgcctgc tcatatttgg gcctgtgtga accccaggct ccgcctcggc ccccaggagc
     8341 cgctcttatt taaaggggat cctcgccttc agtcttcgtc ccagtggaag catcagtctt
     8401 gtgataacaa cttggtgagt cctacagacc agcgctcgac tctaggcttt aaatgcattg
     8461 atttctctgg attagctacc gggagagttt taactttgct tattagtctt gtgctcgaac
     8521 tgctaggttt aaagagtgtt ttctctaatg catgcatgaa gcgacagctg aaaaccagaa
     8581 atagagcgtt tgtcctcctg ttaaactcta gcctttcttt ggcatttgtc agtccgagtc
     8641 ttcagctgcc ggcaggagat tgggtttcag taacacttgc tgagcacaaa ctacacgtgc
     8701 tttcttcaga tttaattcat tattaatcat tctagattta aatgatgttc ttactcattg
     8761 ctgattattt tagccttgcg gagcagatta aatgagcacg tgtttcatta agattgattt
     8821 aaatataatt aatatttcac cactgtagtt ttaaaattgc cttctaagtc atggttgatt
     8881 tttaataggt atcggctcta attagattac tattttggtt tttaaatgtt atttctattt
     8941 catttgtagg ctcgttttta atgtgatttt attagattct agttttaaaa tcatctctta
     9001 gccttaatga gcagactgaa tgaacatgtt tcattaagat taacttcatt ataatgaatt
     9061 atttaatcat tctagtgggt tataaatcag ctctatttaa attaattgtc gtgtttattt
     9121 ctatttaatt tttgattatt ttttcagggt cgtttttatg tgattttttc ccgtttctaa
     9181 ttttaaacta attgtttgat ttttatatga ttcgactctt tttaaatgaa ttgttttgct
     9241 ttttttattg agcatatata tattatacaa tttcaatcaa caattgttgt gctttttaaa
     9301 tgttgtttct atttcatttc tagggtcgat tttattcgat tctagtttta aaatcgtgat
     9361 ttttaacttc taaccttact gagcagactg aatgaacatg tttcatcaag attaacttca
     9421 ttatagttaa ttattgaatc gtgctagtgg tttataaatc agctctattt aaattaatca
     9481 tcatcattat ttctatttaa tttttattat ttctttagga ttatttttat gtgatttttt
     9541 tcacgttcta gttttaaact caatgctcat tactgctcac ccttgctgag cagtctttcg
     9601 ttaagattaa attaattaat aaccattcta gttttaaaac agtgtcttaa ggcatagctg
     9661 atattttaca agtttactat ttaatatatc tcctttcttt ttttagggtc gatttcatct
     9721 gatttgatta atttttatta tcttcactaa gctttttgtt ctaggtttta ttctcagtat
     9781 tattactgtt atatttgaac tgaatttctc ctcatagttt attcatttta tttatttatt
     9841 ttttgtttat tcgctatttt gtattttagt ttttaattag aaattttatt agaatcgttt
     9901 tgaactagtt tttatgcact ctaacattaa tattaaatca taactattat ttttattata
     9961 atttatttgt gtttatcttt ttgtgtagct ttcatgtgtt tttattttag ttttattatg
    10021 tttgcattga attttttgga ctagtttata taacgatata cagaaaagcc atgacaacaa
    10081 atgtttttat aatacctatt aagtataagc taatcagatt tccactattc tgttcaaagt
    10141 taacattatt agccattctg atggatcatt tgacatttgt catatgtctt tatgaacact
    10201 gctctttcag atgccacttt taaagctgtt ttttacagta aatgaacgtt tattattatg
    10261 acattatttc ctaaggcctc tagagtacag caggatgtgg cgtgtgcatt accgtgtaat
    10321 tatgctgact gtaaatgtgc atttatagac tgtcagcatg tctgactgtg cgctgttgtg
    10381 attggtcctg cacagaccaa g
//
