LOCUS       sequence-220538-        8900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized 
            AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 8900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)...
FEATURES             Location/Qualifiers
     source          1..8900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1777..5697
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5417..5426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5698..5745
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5752..5832
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5922..5941)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5987..6042
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6041..6060)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6403..6419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6403..6419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6416..6438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6427..6443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6451..6457,6458..6475,6476..6481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6496..6517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6576..6771
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6622..6757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6777..6911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6814..6833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6868..6887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6979..6996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7150..7738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7230..7249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7909..8700,8701..8769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8532..8551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8770..8874)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctgcagtcg
     1741 acggtaccgc gggcccggga tccaccggtc gccaccatga cacagttcga gggctttacc
     1801 aacctgtatc aggtgagcaa gacactgcgg tttgagctga tcccacaggg caagaccctg
     1861 aagcacatcc aggagcaggg cttcatcgag gaggacaagg cccgcaatga tcactacaag
     1921 gagctgaagc ccatcatcga tcggatctac aagacctatg ccgaccagtg cctgcagctg
     1981 gtgcagctgg attgggagaa cctgagcgcc gccatcgact cctatagaaa ggagaaaacc
     2041 gaggagacaa ggaacgccct gatcgaggag caggccacat atcgcaatgc catccacgac
     2101 tacttcatcg gccggacaga caacctgacc gatgccatca ataagagaca cgccgagatc
     2161 tacaagggcc tgttcaaggc cgagctgttt aatggcaagg tgctgaagca gctgggcacc
     2221 gtgaccacaa ccgagcacga gaacgccctg ctgcggagct tcgacaagtt tacaacctac
     2281 ttctccggct tttatagaaa caggaagaac gtgttcagcg ccgaggatat cagcacagcc
     2341 atcccacacc gcatcgtgca ggacaacttc cccaagttta aggagaattg tcacatcttc
     2401 acacgcctga tcaccgccgt gcccagcctg cgggagcact ttgagaacgt gaagaaggcc
     2461 atcggcatct tcgtgagcac ctccatcgag gaggtgtttt ccttcccttt ttataaccag
     2521 ctgctgacac agacccagat cgacctgtat aaccagctgc tgggaggaat ctctcgggag
     2581 gcaggcaccg agaagatcaa gggcctgaac gaggtgctga atctggccat ccagaagaat
     2641 gatgagacag cccacatcat cgcctccctg ccacacagat tcatccccct gtttaagcag
     2701 atcctgtccg ataggaacac cctgtctttc atcctggagg agtttaagag cgacgaggaa
     2761 gtgatccagt ccttctgcaa gtacaagaca ctgctgagaa acgagaacgt gctggagaca
     2821 gccgaggccc tgtttaacga gctgaacagc atcgacctga cacacatctt catcagccac
     2881 aagaagctgg agacaatcag cagcgccctg tgcgaccact gggatacact gaggaatgcc
     2941 ctgtatgagc ggagaatctc cgagctgaca ggcaagatca ccaagtctgc caaggagaag
     3001 gtgcagcgca gcctgaagca cgaggatatc aacctgcagg agatcatctc tgccgcaggc
     3061 aaggagctga gcgaggcctt caagcagaaa accagcgaga tcctgtccca cgcacacgcc
     3121 gccctggatc agccactgcc tacaaccctg aagaagcagg aggagaagga gatcctgaag
     3181 tctcagctgg acagcctgct gggcctgtac cacctgctgg actggtttgc cgtggatgag
     3241 tccaacgagg tggaccccga gttctctgcc cggctgaccg gcatcaagct ggagatggag
     3301 ccttctctga gcttctacaa caaggccaga aattatgcca ccaagaagcc ctactccgtg
     3361 gagaagttca agctgaactt tcagatgcct acactggcca gaggctggga cgtgaataag
     3421 gagaagaaca atggcgccat cctgtttgtg aagaacggcc tgtactatct gggcatcatg
     3481 ccaaagcaga agggcaggta taaggccctg agcttcgagc ccacagagaa aaccagcgag
     3541 ggctttgata agatgtacta tgactacttc cctgatgccg ccaagatgat cccaaagtgc
     3601 agcacccagc tgaaggccgt gacagcccac tttcagaccc acacaacccc catcctgctg
     3661 tccaacaatt tcatcgagcc tctggagatc acaaaggaga tctacgacct gaacaatcct
     3721 gagaaggagc caaagaagtt tcagacagcc tacgccaaga aaaccggcga ccagaagggc
     3781 tacagagagg ccctgtgcaa gtggatcgac ttcacaaggg attttctgtc caagtatacc
     3841 aagacaacct ctatcgatct gtctagcctg cggccatcct ctcagtataa ggacctgggc
     3901 gagtactatg ccgagctgaa tcccctgctg taccacatca gcttccagag aatcgccgag
     3961 aaggagatca tggatgccgt ggagacaggc aagctgtacc tgttccagat ctataacaag
     4021 gactttgcca agggccacca cggcaagcct aatctgcaca cactgtattg gaccggcctg
     4081 ttttctccag agaacctggc caagacaagc atcaagctga atggccaggc cgagctgttc
     4141 taccgcccta agtccaggat gaagaggatg gcacaccggc tgggagagaa gatgctgaac
     4201 aagaagctga aggatcagaa aaccccaatc cccgacaccc tgtaccagga gctgtacgac
     4261 tatgtgaatc acagactgtc ccacgacctg tctgatgagg ccagggccct gctgcccaac
     4321 gtgatcacca aggaggtgtc tcacgagatc atcaaggata ggcgctttac cagcgacaag
     4381 ttctttttcc acgtgcctat cacactgaac tatcaggccg ccaattcccc atctaagttc
     4441 aaccagaggg tgaatgccta cctgaaggag caccccgaga cacctatcat cggcatcgat
     4501 cggggcgaga gaaacctgat ctatatcaca gtgatcgact ccaccggcaa gatcctggag
     4561 cagcggagcc tgaacaccat ccagcagttt gattaccaga agaagctgga caacagggag
     4621 aaggagaggg tggcagcaag gcaggcctgg tctgtggtgg gcacaatcaa ggatctgaag
     4681 cagggctatc tgagccaggt catccacgag atcgtggacc tgatgatcca ctaccaggcc
     4741 gtggtggtgc tggagaacct gaatttcggc tttaagagca agaggaccgg catcgccgag
     4801 aaggccgtgt accagcagtt cgagaagatg ctgatcgata agctgaattg cctggtgctg
     4861 aaggactatc cagcagagaa agtgggaggc gtgctgaacc cataccagct gacagaccag
     4921 ttcacctcct ttgccaagat gggcacccag tctggcttcc tgttttacgt gcctgcccca
     4981 tatacatcta agatcgatcc cctgaccggc ttcgtggacc ccttcgtgtg gaaaaccatc
     5041 aagaatcacg agagccgcaa gcacttcctg gagggcttcg actttctgca ctacgacgtg
     5101 aaaaccggcg acttcatcct gcactttaag atgaacagaa atctgtcctt ccagaggggc
     5161 ctgcccggct ttatgcctgc atgggatatc gtgttcgaga agaacgagac acagtttgac
     5221 gccaagggca cccctttcat cgccggcaag agaatcgtgc cagtgatcga gaatcacaga
     5281 ttcaccggca gataccggga cctgtatcct gccaacgagc tgatcgccct gctggaggag
     5341 aagggcatcg tgttcaggga tggctccaac atcctgccaa agctgctgga gaatgacgat
     5401 tctcacgcca tcgacaccat ggtggccctg atccgcagcg tgctgcagat gcggaactcc
     5461 aatgccgcca caggcgagga ctatatcaac agccccgtgc gcgatctgaa tggcgtgtgc
     5521 ttcgactccc ggtttcagaa cccagagtgg cccatggacg ccgatgccaa tggcgcctac
     5581 cacatcgccc tgaagggcca gctgctgctg aatcacctga aggagagcaa ggatctgaag
     5641 ctgcagaacg gcatctccaa tcaggactgg ctggcctaca tccaggagct gcgcaacaaa
     5701 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc ctacccatac
     5761 gatgttccag attacgctta tccctacgac gtgcctgatt atgcataccc atatgatgtc
     5821 cccgactatg cctaaagcgg ccgcactcct caggtgcagg ctgcctatca gaaggtggtg
     5881 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     5941 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     6001 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     6061 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     6121 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     6181 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     6241 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     6301 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     6361 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     6421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6541 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     6601 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6661 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6721 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     6781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6841 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6901 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6961 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7021 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7081 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7321 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7441 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7501 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7561 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7621 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7681 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7741 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7801 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7861 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7921 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7981 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8041 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8101 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8161 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8221 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8281 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8341 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8401 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8461 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8521 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8581 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8641 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8701 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8761 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8881 tccccgaaaa gtgccacctg
//
