LOCUS       sequence-220541-        9592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab11ba.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab11ba
FEATURES             Location/Qualifiers
     source          1..9592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1416..1436)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1444..1460)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1476..1610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1500..1519)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1554..1573
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2049..2236,2237..2248)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2282..2298)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2282..2298)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2335..2355)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2335..2353)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2374..2390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2387..2409)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2398..2414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2422..2428,2429..2446,2447..2452))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2467..2488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2605..2622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2776..3364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2856..2875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3535..4326,4327..4395))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4158..4177
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4396..4500)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4663..4684)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5033..5044,5045..5207)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5580..5596
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5616..5636
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtggggacc cgtgacgacg aatacgatta
      781 tttatttaaa gtggttctca ttggagactc tggggtgggg aagagtaacc tgttgtcccg
      841 tttcactcgg aacgagttta acctggagag caagagcacc attggtgtgg agttcgcaac
      901 ccgcagcatc caagtggacg ggaagaccat taaagctcag atctgggata cagcaggaca
      961 ggaacgctac agagcaataa catcagcgta ctaccggggt gcagttggag ctcttctggt
     1021 ctacgacatc gcaaaacatc tgacttacga gaatgtggag cgctggctga aggagctgcg
     1081 ggatcatgcg gataacaaca tcgtcatcat gctggtgggc aacaagagcg acctgaggca
     1141 cctgagggcc gtgcccactg atgaggccag agcttttgca gagaagaaca atctctcctt
     1201 cattgaaaca tcggcgttgg actccacaaa tgtggaggag gcatttaaaa acatactaac
     1261 agaaatctat cggattgtgt cacagaaaca gatggtcgac aggcccgggc acgatgagtc
     1321 tcctggcaat aacgtagtgg acatcagcgt gccgcccact actgacgggt taaagggcaa
     1381 caaatttcag tgctgtcaga acctgtgacc caacacaact ttattataca tagttgataa
     1441 ttcactggcc gtcgttttac ggtaccatcg atgatgatcc agacatgata agatacattg
     1501 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     1561 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     1621 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     1681 aaaacctcta caaatgtggt atggctgatt atgatcctct agatcagatc tgcgaagata
     1741 cggccacggg tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga
     1801 tgaatcacat acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc
     1861 caggtcaagg tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc
     1921 aatgaacatg tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat
     1981 aacatttaaa ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa
     2041 atagaatgaa gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga
     2101 gtaaaaagta cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt
     2161 taattgtact caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt
     2221 aaaattactc aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc
     2281 agatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
     2341 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2401 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2461 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2521 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2581 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2641 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2701 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2761 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2821 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2881 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2941 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3001 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3061 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3121 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3181 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3241 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3301 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3361 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3421 ttaaggcatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3481 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3541 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3601 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3661 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3721 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3781 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3841 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3901 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3961 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4021 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4081 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4141 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4201 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4261 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4321 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4381 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4441 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4501 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     4561 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     4621 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4681 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     4741 ggcccacgct ggctaagaac tcatcagcct ccccggtcca tctacccacg taccaatgca
     4801 ccaattggcc acaatgacgg ctactacatg gtgccattcc ttcctcttta taggaatgga
     4861 gactacctcc tgtccaacaa tgctcttgga tacgagtacg cctacctgtt ggacccaggt
     4921 cattgcacaa caccagaaat gccctctgat ctgcaaaaga cgtgaatatc tgttcagaca
     4981 cccatatcca ctctgttcca cacaggtcag aggtttgtcc aggagttctt gacagaggtg
     5041 taaaaagtac tcaaaaattt tactcaagtg aaagtacaag tacttaggga aaattttact
     5101 caattaaaag taaaagtatc tggctagaat cttacttgag taaaagtaaa aaagtactcc
     5161 attaaaattg tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga
     5221 ttcttgttta agcttttaat ctcaaaaaac attaaatgaa atgcatacaa ggttttatcc
     5281 tgctttagaa ctgtttgtat ttaattatca aactataaga cagacaatct aatgccagta
     5341 cacgctactc aaagttgtaa aacctcagat ttaacttcag tagaagctga ttctcaaaat
     5401 tgttagtgtc aagcctagct cttttggggc tgaaaagcaa tcctgcagtg ctgaaaagcc
     5461 tctcacaggc agccgatgcg ggaagaggtg tattagtctt gatagagagg ctgcaaatag
     5521 caggaaacgt gagcagagac tccctggtgt ctgaaacaca ggccagatgg gccctcgagc
     5581 aggaaacagc tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttggcac
     5641 gcgcagattc cgtgtgggcc tggtaatatc ttaccatttt aagctatttt aagattaggg
     5701 aaaaaaatta aataaaacta ctttaaaatt actttcctta aaaagcaaca tatttattta
     5761 gtttttgttt aactggctca agattgtaat attttacttt tacatgtttt tttaagtacc
     5821 taaatgtgtt caaaagtaaa gtatgttatt gttttttttt tttaatgtta gctaagcttt
     5881 aaattgtaat atattacttt taaaagtgac tttttaaaaa tgtaaataaa aaagtaacta
     5941 taaatgtgta atgcatgtta tttctgtggg tttttaattt tcatctcaac tgggcttaca
     6001 attttaacat aataaaaatc aaatcaaatc aaatcaggcc cttatttgtc acatacactc
     6061 ttgtatactg aaataaacta aatgttgaga aaaatttaaa tacaccaaaa taacgcatac
     6121 cttttttgta attacctaag taaaacattt agttcctttt ttaagtgact caagattgta
     6181 atctattaat tgtaaaagat aaaactagtg atcaatctga cgtgcttcta tgcagcctgt
     6241 gagattaatt gtgatgttta attattaaac aatttaaatg gatcaaacat ttcacatgtg
     6301 cttattgtgt ccaacaaaac cagcagaagc acttaaaaat attgcatgac aacatgagtt
     6361 tttaatgtaa attatatata cttcctaaac gaagttaatc atatttctaa aataataatg
     6421 tcattgaaat atttaaataa atgataagct ttagttgatt caacataaaa ttgaaagcaa
     6481 taaggacttt ttttttttac agcggtgaag caaaagcatg taataatcat ataatataat
     6541 aaataaataa ataaataatg gctcttggtg caaatatgaa cactttaaat aaatatagaa
     6601 taaatatgat caataagtca taacgatgtg ttttatgttg attataattt aaacatgttt
     6661 ctaacgcaag ttttaaaaac tgttttaaag tattatttta agttataaga tcaatttgac
     6721 tcaacatttt gtagtgaaaa tatgttgtgt aaaattaata taatatacac tataaaaatg
     6781 cagggttcca cacaatacat taatgttgtc ccaacacaaa tcgattaagt taacaaatat
     6841 atgtggattg aacataaaaa attaagttgt cccaatgaaa tctcaagaat tgtgttgttt
     6901 cagctcattt taaataagta gttttaacaa gtaaaaaata acatattttt gagtgtgtat
     6961 atatatatat tacaaaatat aataatataa aatgtaaaag atataaacca tgctacatat
     7021 atttaagagc tattttgcac attacttttt tgcaagccat tttagtgttt tttttacaaa
     7081 tatatattag ctaaatatac aatatcttaa atatacaatt aatcagacat gcaattaatg
     7141 catttaacct acataaaaat acatgatcaa catatgtttt atgctgatta caactaaatc
     7201 aaacgtaatg aaattaattt ataaaagcga tttaaatatg tcaatatttt aagttaaaat
     7261 cacttgaaac ctactataaa tgaacttaaa ttaaaaaaat ctcctttaat tccaatgcaa
     7321 cagtgttgtt ttttcagctg aggacccccg gtgcagatgg ttggcccccg gcccgctatt
     7381 ctaaggaagc ctttcttaat tttagggcgg cgctgcatgc gctcagatca gattcacggg
     7441 tggagagggt tattttctct tttttatttt ttcttgcctg ctcatatttg ggcctgtgtg
     7501 aaccccaggc tccgcctcgg cccccaggag ccgctcttat ttaaagggga tcctcgcctt
     7561 cagtcttcgt cccagtggaa gcatcagtct tgtgataaca acttggtgag tcctacagac
     7621 cagcgctcga ctctaggctt taaatgcatt gatttctctg gattagctac cgggagagtt
     7681 ttaactttgc ttattagtct tgtgctcgaa ctgctaggtt taaagagtgt tttctctaat
     7741 gcatgcatga agcgacagct gaaaaccaga aatagagcgt ttgtcctcct gttaaactct
     7801 agcctttctt tggcatttgt cagtccgagt cttcagctgc cggcaggaga ttgggtttca
     7861 gtaacacttg ctgagcacaa actacacgtg ctttcttcag atttaattca ttattaatca
     7921 ttctagattt aaatgatgtt cttactcatt gctgattatt ttagccttgc ggagcagatt
     7981 aaatgagcac gtgtttcatt aagattgatt taaatataat taatatttca ccactgtagt
     8041 tttaaaattg ccttctaagt catggttgat ttttaatagg tatcggctct aattagatta
     8101 ctattttggt ttttaaatgt tatttctatt tcatttgtag gctcgttttt aatgtgattt
     8161 tattagattc tagttttaaa atcatctctt agccttaatg agcagactga atgaacatgt
     8221 ttcattaaga ttaacttcat tataatgaat tatttaatca ttctagtggg ttataaatca
     8281 gctctattta aattaattgt cgtgtttatt tctatttaat ttttgattat tttttcaggg
     8341 tcgtttttat gtgatttttt cccgtttcta attttaaact aattgtttga tttttatatg
     8401 attcgactct ttttaaatga attgttttgc tttttttatt gagcatatat atattataca
     8461 atttcaatca acaattgttg tgctttttaa atgttgtttc tatttcattt ctagggtcga
     8521 ttttattcga ttctagtttt aaaatcgtga tttttaactt ctaaccttac tgagcagact
     8581 gaatgaacat gtttcatcaa gattaacttc attatagtta attattgaat cgtgctagtg
     8641 gtttataaat cagctctatt taaattaatc atcatcatta tttctattta atttttatta
     8701 tttctttagg attattttta tgtgattttt ttcacgttct agttttaaac tcaatgctca
     8761 ttactgctca cccttgctga gcagtctttc gttaagatta aattaattaa taaccattct
     8821 agttttaaaa cagtgtctta aggcatagct gatattttac aagtttacta tttaatatat
     8881 ctcctttctt tttttagggt cgatttcatc tgatttgatt aatttttatt atcttcacta
     8941 agctttttgt tctaggtttt attctcagta ttattactgt tatatttgaa ctgaatttct
     9001 cctcatagtt tattcatttt atttatttat tttttgttta ttcgctattt tgtattttag
     9061 tttttaatta gaaattttat tagaatcgtt ttgaactagt ttttatgcac tctaacatta
     9121 atattaaatc ataactatta tttttattat aatttatttg tgtttatctt tttgtgtagc
     9181 tttcatgtgt ttttatttta gttttattat gtttgcattg aattttttgg actagtttat
     9241 ataacgatat acagaaaagc catgacaaca aatgttttta taatacctat taagtataag
     9301 ctaatcagat ttccactatt ctgttcaaag ttaacattat tagccattct gatggatcat
     9361 ttgacatttg tcatatgtct ttatgaacac tgctctttca gatgccactt ttaaagctgt
     9421 tttttacagt aaatgaacgt ttattattat gacattattt cctaaggcct ctagagtaca
     9481 gcaggatgtg gcgtgtgcat taccgtgtaa ttatgctgac tgtaaatgtg catttataga
     9541 ctgtcagcat gtctgactgt gcgctgttgt gattggtcct gcacagacca ag
//
