LOCUS       sequence-220550-       10308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-326
FEATURES             Location/Qualifiers
     source          1..10308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     555..574
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(594..671)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     877..896
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     980..999
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        980..998
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    999..1023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1077..1094
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4642..4731
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4744..4833
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(6222..6240)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6236..6283
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6320..6775
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6407..6426)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6617..6638
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6867..7682)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6867..7682)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7590..7609
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7674..7693
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7804..8392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8293..8312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8546..8563
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8578..8717
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8643..8662)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8803..8825
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8819..9010)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9819..10308,1..593))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
       61 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cctcatggga gaaaataata
      121 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
      181 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
      241 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
      301 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
      361 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
      421 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
      481 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
      541 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
      601 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
      661 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
      721 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
      781 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
      841 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
      901 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
      961 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
     1021 tcccctctag aaataatttt gccttaactt taagaaggag atataccatg ggcagccacc
     1081 accaccacca ccacagcgga atgcagatct ttgttaagac tctcaccgga aagacaatca
     1141 ccctagaggt ggaaagctcc gacaccatcg acaacgttaa ggccaagatc caggataagg
     1201 agggcattcc tccggatcag cagaggctta ttttcgccgg caagcagcta gaggatggcc
     1261 gtacgttggc tgattacaat atccagaagg aatccaccct ccacttggtc ctcaggctcc
     1321 gtggtggtta agcttgcaat taactttaag aaggagatat acaatgcttc acaagcgagc
     1381 tagtgaagct aacgataaga acgataatac catcatcggt tccgatttag catcatctaa
     1441 gaaacgccgt atcgatttca ctgaatcttc gtctgataag tcatcttcga ttttagcttc
     1501 tggtagtagc aggggttttc acggcgatag cgtcgttcag caaatcgaca tggcttttgg
     1561 taattcgaac cgtcaggaga ttgatgaaga tctgcacagt cgccagctcg ccgtctatgg
     1621 tcgtgagact atgaggcgtc tctttgcttc caatgttctc atctcgggga tgcacggtct
     1681 tggtgccgag attgccaaga atcttatact tgctggtgtg aagtctgtga ccttgcatga
     1741 tgaaagagtt gtagagctat gggacttatc aagcaacttt gttttctcgg aggatgatgt
     1801 tggcaagaat agagcagatg cttctgttca gaagttacag gatcttaaca atgctgtggt
     1861 tgtctctagc ttgaccaaaa gcttaaacaa agaggatctt tctggcttcc aggttgttgt
     1921 attctctgac ataagcatgg aaagagcaat cgaatttgat gactattgtc acagccatca
     1981 gcctcctatc gcttttgtca aggctgatgt caggggtctt ttcggctctg tcttttgtga
     2041 ttttgggcct gaatttgcgg ttcttgatgt tgatggagag gaaccccata caggcatcat
     2101 tgcgtctatc tctaatgaga accaggcatt tatttcctgt gtcgacgatg agaggcttga
     2161 gtttgaagat ggtgacctag ttgttttctc agaagttgag ggtatgacag aattgaatga
     2221 tgggaaacca aggaagataa aaagtacacg gccatattca ttcacccttg atgaggacac
     2281 tactaactat ggaacatatg tgaagggtgg tattgtcact caagtgaaac agccaaagtt
     2341 actgaatttc aagccattga gggaagcgct taaggatcca ggggattttc tatttagtga
     2401 tttctctaag tttgatcggc ctccactcct tcacttagca ttccaggcac ttgatcattt
     2461 taaagctgaa gctggcagat ttcctgttgc tggctcagaa gaggacgctc agaagcttat
     2521 atcgattgcc acagccatca ataccgggca gggtgattta aaggtggaga atgttgatca
     2581 gaagcttcta agacactttt cctttggagc caaggctgtc ctgaatccta tggctgcaat
     2641 gtttggcggt attgttggac aggaggttgt taaagcttgc tctggaaaat ttcatcctct
     2701 cttccagttt ttctactttg attcagtgga gtcactcccc tctgaacctg tggactcgag
     2761 tgactttgca ccaagaaaca gccggtatga tgcccaaata tctgtatttg gggccaagtt
     2821 ccagaagaaa cttgaagatg ctaaagtttt cacagtaggg tctggtgctc ttggctgtga
     2881 gttcttgaaa aatttggctc tgatgggggt ttcttgtgga agccaaggga agctgacagt
     2941 gacagatgat gatatcattg agaagagtaa cctcagtcgt caatttctgt tccgtgactg
     3001 gaacattgga caggctaaat ccacagttgc tgcttcggct gctgctgtta taaatcctcg
     3061 cttcaacatt gaggccctgc aaaaccgtgt tggcgcggag actgagaatg tatttgacga
     3121 tgccttctgg gagaacttaa ctgttgtcgt caatgcactg gataatgtta atgcgaggct
     3181 ctatgttgat tcgaggtgct tgtatttcca gaagcctctc cttgagtctg gaactcttgg
     3241 tacaaagtgc aacacacagt cggttatccc acatctaact gaaaattacg gtgcctcaag
     3301 ggacccacca gagaaacagg cccccatgtg tacagtgcat tccttcccgc ataacattga
     3361 tcactgttta acttgggctc gctctgagtt tgagggtctg ctggagaaaa ctcccgctga
     3421 agtgaatgca tatctttcta gcccagttga gtacactaac tcaatgatga gtgctggtga
     3481 tgctcaggcg agggacacat tggagaggat tgttgagtgc cttgaaaagg agaagtgtga
     3541 aaccttccaa gactgcttaa cctgggcacg actgaggttt gaggattact ttgtgaaccg
     3601 tgtcaagcaa ttgatataca catttcctga agatgctgcg actagtaccg gagctccatt
     3661 ctggtctgct ccaaaaagat tccctcgtcc actccagtac tcgtcgtccg acccaagtct
     3721 ccttaacttt atcacagcga ctgctatttt acgagcagag acatttggga tcccgatacc
     3781 tgagtggact aagaacccaa aggaagcagc agaagctgtt gacagagtga tagtcccaga
     3841 ctttgagcca aggcaagatg caaagattgt gactgatgag aaagctacca ctttaaccac
     3901 tgcttcggtg gacgacgctg cagtcatcga cgatctcatt gctaagatcg accagtgtag
     3961 gcataacttg tcgccagact tcaggatgaa acctattcag ttcgaaaagg atgatgatac
     4021 aaactatcac atggatgtga tagcaggact agccaacatg agggcgagaa attacagcat
     4081 ccctgaagtg gacaagctga aagcaaagtt catagcaggg agaatcatcc cagccattgc
     4141 gacttcaaca gccatggcta ctgggctggt ctgcctcgag ctgtacaagg tccttgatgg
     4201 aggacataaa gtggaagctt acagaaacac atttgccaac ctggcacttc cactattctc
     4261 catggctgaa ccacttccac caaaggtggt gaagcaccgt gacatggctt ggacagtttg
     4321 ggacagatgg gttctcaagg gaaacccaac tcttcgtgag gtgttgcagt ggctggagga
     4381 caaagggctt agcgcataca gcatctcttg tggaagctgt ctactgttca acagtatgtt
     4441 cacaaggcac aaggagagga tggacaagaa ggttgtggat ctcgctcggg atgttgcgaa
     4501 agtcgagtta ccgccttatc gcaaccatct tgatgtagtg gtggcttgtg aggatgaaga
     4561 tgacaatgac gtcgatattc ctctcgtctc tatctacttc aggtgagctt actattaact
     4621 ttaagaagga gatataccat gtacccatac gatgttcctg actatgcggg ctatccctat
     4681 gacgtcccgg actatgcagg attgtatcca tatgacgttc cagattacgc cactagagct
     4741 gcttacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca
     4801 ggattgtatc catatgacgt tccagattac gcaatggctc aagcagcgag attaagcctg
     4861 agaatgcaga aagagctaaa gcttctcctc tccgatccac ctcacggcgc ttcgtttcca
     4921 catctctctt ccgccgccaa tggctccggc gatttctcca ctttctccac catcgatgct
     4981 caaatcgaag gtcctgaaga tactgtatat gctaatggaa tcttcaattt gaagattcaa
     5041 atccctgaga gatatccatt tcagccacca attgtgtcgt tcgctacacc gatttatcac
     5101 ccaaacatcg ataacagtgg ccggatttgc cttgacattc taaatctccc tcccaaggga
     5161 gcttggcaac catcgttgaa tatctcgaca gttttgacga gtatgagatt gttgcttagt
     5221 gaaccaaatc ctgatgatgg attgatgtgt gaagtaagca gggagtataa atacaataga
     5281 caaacttttg attataaggc acgagagatg actgagaagt atgctaaggt taaagctgat
     5341 ggatgcagca ccagcttaca aattaagaat catggagatg agaagtctgg tgaaagtgga
     5401 aatagtgtta agcttaagtt gacagtggaa tcttctttaa gcatagcaca tacagtgaaa
     5461 cgtgagactg cagggagaga tgaacaagag gatggcaatg ggaaaagaaa agcagtggtt
     5521 ggttttggcg aggggaattc gtttggaaac gatgggatta aaaggagtag gaagaagtta
     5581 tctcttgcat tgccttctca atctcaaaag aaagacttgt gtggtgaaga agagctcaca
     5641 cgtggtgttt cagctgcttg caaagaaaac aaaaaaccaa atttgaatgg aaagaagctt
     5701 tctctggggc tgaagcagcc atgtaatgat actttggcga gttttcgaac ttctgctgcg
     5761 aagagtgaca acaatagact aagccgaaaa ctgtccctga gatcaccact tggagagttg
     5821 aatgaagtta gcaaacctga ggtacttgcg caaactgata tgaaactgga gatgaatcaa
     5881 aatgaagatg caagaagttt gcgaggtgag tttgagaatt cggtattgga agaaacatca
     5941 atggctgaat ccattgttgt tctagacagt gatgatagtg gacaagaaga agaagaaaga
     6001 gtctcttctt cgaggtcaag attattactg gcaaagagga gagtcctcaa atgtagacct
     6061 tgagctttta cgcttttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     6121 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgggat gagatccggc
     6181 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     6241 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     6301 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6361 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6421 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6481 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6541 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6601 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6661 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6721 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     6781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6841 atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc
     6901 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     6961 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     7021 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     7081 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt
     7141 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     7201 accaaaccgt tattcattcg tgattgcgcc tgagcgagtc gaaatacgcg atcgctgtta
     7261 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     7321 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     7381 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     7441 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     7501 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     7561 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     7621 gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc
     7681 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgaccaaaat
     7741 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7801 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7861 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7921 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     7981 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8041 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8101 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8161 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8221 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8281 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8341 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8401 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8461 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8521 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     8581 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc
     8641 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     8701 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8761 gtctgctccc ggcatccgct tacagacaag ctgtgaccga ctccgggagc tgcatgtgtc
     8821 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     8881 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     8941 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     9001 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     9061 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     9121 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     9181 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     9241 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     9301 tttacgaaac acggaaaccc aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     9361 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     9421 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtgggg
     9481 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
     9541 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
     9601 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
     9661 ctacgagttg catgataaag aacacagtca taagtgcggc gacgatagtc atgccccgcg
     9721 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
     9781 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9901 gtattgggcg ccagggtggt ttttcttttc accagtgaca cgggcaacag ctgattgccc
     9961 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
    10021 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ctcggtatcg
    10081 tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc
    10141 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
    10201 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
    10261 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagcc
//
